Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	756,119	C→A	D71E (GAC→GAA)	sdhA →	succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	756,119	0	C	A	100.0%	78.7 / NA	24	D71E (GAC→GAA)	sdhA	succinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (12/12);  total (12/12)

CGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAAC  >  NC_000913/755974‑756255                                                                                                                                                  |                                                                                                                                         cgcTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGAAAcc                                                                                                                                       <  8:79768/149‑1 (MQ=255)   cTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGAAACCGt                                                                                                                                     >  2:57371/1‑149 (MQ=255)                      gCCAGACCTGTGCGCTGCTCTCTAAAGTATTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGAAACCGTGAAAGGGTCGGACTATATc                                                                                                                  >  5:143/1‑149 (MQ=255)                           aCCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGAAACCGTGAAAGGGTCGGACTATATCGGTGa                                                                                                             >  7:33009/1‑149 (MQ=255)                             cTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGAAACCGTGAAAGGGTCGGACTATATCGGTGAcc                                                                                                           >  2:144674/1‑149 (MQ=255)                               gtgCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGAAACCGTGAAAGGGTCGGACTATATCGGTGACCAg                                                                                                         >  1:23943/1‑149 (MQ=255)                                       ctctctAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGAAACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGa                                                                                                  <  6:57282/148‑1 (MQ=255)                                          tctAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGAAACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGAtt                                                                                                <  2:23943/147‑1 (MQ=255)                                            tAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGAAACCGTGAAAGGGTCGGACTATATCGGTGACCAGGAcgc                                                                                                    <  4:21368/141‑1 (MQ=255)                                            tAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGAAACCGTGAAAGGGTCGGACTATATCGGTGACCAGGAcgc                                                                                                    >  3:21368/1‑141 (MQ=255)                                                           ccGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGAAACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCg                                                                             >  5:100360/1‑149 (MQ=255)                                                            cGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGAAACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCgg                                                                            <  1:57371/149‑1 (MQ=255)                                                                 ccATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGAAACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCg                                                                        >  6:149066/1‑148 (MQ=255)                                                                         tttCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGAAACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGAtt                                                               <  8:33009/149‑1 (MQ=255)                                                                               cgcAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGTCATATGTACGAAACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCTGAAGCGATTCTGGaa                                                         <  6:143/149‑1 (MQ=255)                                                                               cgcAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGAAACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTg                                                            <  5:149066/146‑1 (MQ=255)                                                                                gcAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGAAACCGTGAGAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGAAAAACCGGGCCGGAAGCGATTCTGGAAc                                                        >  1:106064/1‑149 (MQ=255)                                                                                           ttACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGAAACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATg                                             <  1:144674/149‑1 (MQ=255)                                                                                             aCCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGAAACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGAAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATgg                                            <  2:106064/148‑1 (MQ=255)                                                                                              ccGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGAAACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGc                                          >  1:55004/1‑149 (MQ=255)                                                                                                           tAATACCCATGAAGATAACTGGGAATGGCATATGTACGAAACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTcgc                             >  6:75578/1‑149 (MQ=255)                                                                                                                         aTAACTGGGAATGGCATATGTACGAAACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGt               <  5:75578/149‑1 (MQ=255)                                                                                                                             cTGGGAATGGCATATGTACGAAACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGta           <  2:55004/149‑1 (MQ=255)                                                                                                                                      gCATATGTACGAAACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAAc  >  6:142206/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                         CGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAAC  >  NC_000913/755974‑756255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑