Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 564,107 IS2 (+) +5 bp coding (370‑374/633 nt) sfmZ ← response regulator family protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 381260 =NA (NA)116 (1.060) 92/284 0.1 100% noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 564111 0 (0.000)coding (370/633 nt) sfmZ response regulator family protein
* ? NC_000913 = 382590NA (NA)111 (1.010) 72/286 0.4 100% noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 564107 = 0 (0.000)coding (374/633 nt) sfmZ response regulator family protein

Only 100 of 231 total aligned reads displayed.

TAACTTATCGGCAATTTCTTTATTAGATAATCCGCTAACCAGATAACGTAATATGGTCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCT  >  NC_000913/563959‑564255
                                                                                                                                                    |                                                                                                                                                    
caaagaaccttgcagaggcgttcgagcattataacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccag                                                                                                                                                      <  5:24480‑M2/1‑1 (MQ=255)
 aaagaaccttgcagaggcgttcgagcattataacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccagt                                                                                                                                                     <  6:99020‑M2/2‑1 (MQ=255)
 aaagaaccttgcagaggcgttcgagcattataacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccagt                                                                                                                                                     <  4:375533‑M2/2‑1 (MQ=255)
 aaagaaccttgcagaggcgttcgagcattataacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccagt                                                                                                                                                     >  1:381268‑M2/148‑149 (MQ=255)
   cTTATCGGCAATTTCTTTATTAGATACTCCGCTAACCAGATAACGTAATATGGTCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACgtgt                                                                                                                                                   <  8:441988/149‑1 (MQ=255)
    ttATCGGCAATTTCTTTATTAGATAATCCGCTAACCAGATAACGTAATATGGTCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTa                                                                                                                                                  >  3:188935/1‑149 (MQ=255)
      aTCGGCAATTTCTTTATTAGATAATCCGCTAACCAGATAACGTAATATGGTCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTa                                                                                                                                                  <  6:161035/147‑1 (MQ=255)
        cttgcagaggcgttcgagcattataacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCg                                                                                                                                              >  5:1573‑M2/141‑149 (MQ=255)
        cttgcagaggcggtcgagcattataacgactggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCg                                                                                                                                              <  2:430117‑M2/9‑1 (MQ=255)
         ggCAATTTCTTTATTAGATAATCCGCTAACCAGATAACGTAATATGGTCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtaga                                                                                                                                              >  6:364450‑M2/1‑144 (MQ=255)
          gCAATTTCTTTATTAGATAATCCGCTAACCAGATAACGTAATATGGTCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagact                                                                                                                                            <  6:207557‑M2/149‑7 (MQ=255)
          gCAATTTCTTTATTAGATAATCCGCTAACCAGATAACGTAATATGGTCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagact                                                                                                                                            <  8:359388‑M2/149‑7 (MQ=255)
             aTTTCTTTATTAGATAATCCGCTAACCAGATAACGTAATATGGTCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggc                                                                                                                                         <  3:3644‑M2/149‑10 (MQ=255)
              gaggcgttcgagcattataacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAgg                                                                                                                                        <  5:174472‑M2/15‑1 (MQ=255)
                    ttcgagcattataacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGatcatc                                                                                                                                  <  1:220958‑M2/21‑1 (MQ=255)
                    tattaGATAATCCGCTAACCAGATAACGTAATATGGTCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccc                                                                                                                                     >  8:200047‑M2/1‑133 (MQ=255)
                     attaGATAATCCGCTAACCAGATAACGTAATATGGTCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaa                                                                                                                                 <  5:50852‑M2/149‑18 (MQ=255)
                      ttaGATAATCCGCTAACCAGATAACGTAATATGGTCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCTTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaat                                                                                                                                <  2:410228‑M2/149‑19 (MQ=255)
                      ttaGATAATCCGCTAACCAGATAACGTAATATGGTCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaat                                                                                                                                <  2:351349‑M2/149‑19 (MQ=255)
                      ttaGATAATCCGCTAACCAGATAACGTAATATGGTCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaa                                                                                                                                 >  3:211521‑M2/1‑131 (MQ=255)
                       taGATAATCCGCTAACCAGATAACGTAATATGGTCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatc                                                                                                                               <  6:277025‑M2/149‑20 (MQ=255)
                            ttataacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGc                                                                                                                          <  6:109245‑M2/29‑1 (MQ=255)
                              tCCGCTAACCAGATAACGTAATATGGTCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccaga                                                                                                                         <  6:341004‑M2/148‑26 (MQ=255)
                              tCCGCTAACCAGATAACGTAATATGGTCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccaga                                                                                                                         <  4:341272‑M2/148‑26 (MQ=255)
                               taacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCAt                                                                                                                        <  8:264734‑M2/31‑1 (MQ=255)
                                cGCTAACGAGATACCGTAATATCGTCACTTCTCGATTCGATAGCACTGGGAGCGTTGAACTATTCGTACTACATTTATTGCTTTTTATTTAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctcctgtcaa                                                                                                                      >  4:418745‑M2/1‑121 (MQ=255)
                                  cTAACCAGATAACGTAATATGGTCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaacc                                                                                                                    >  2:330216‑M2/1‑119 (MQ=255)
                                   gaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGaaa                                                                                                                    >  7:2239‑M2/114‑148 (MQ=255)
                                          aTAACGTAATATGGTCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagtcaaccaatatcac                                                                                                            <  5:32560‑M2/149‑39 (MQ=255)
                                            aaCGTAATATGGTCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcact                                                                                                           <  4:241702‑M2/148‑40 (MQ=255)
                                            aaCGTAATATGGTCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcact                                                                                                           <  7:169120‑M2/148‑40 (MQ=255)
                                            aaCGTAATATGGTCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccct                                                                                                                                    <  8:142939‑M2/123‑15 (MQ=255)
                                            aaCGTAATATGGTCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccct                                                                                                                                    >  7:142939‑M2/1‑109 (MQ=255)
                                                   tatGGTCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctacagacaaccaatataacttaaataag                                                                                                   <  1:452752‑M2/149‑48 (MQ=255)
                                                        ctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCAt                                                                                              <  6:218566‑M2/57‑1 (MQ=255)
                                                            ttCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaa                                                                                                       >  5:427166‑M2/1‑93 (MQ=255)
                                                            ttCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaa                                                                                                       <  6:427166‑M2/136‑44 (MQ=255)
                                                              cACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtctt                                                                                         <  2:60640‑M2/148‑58 (MQ=255)
                                                                 gATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctacagacaaacaatatcacttaaataagtgatagtcttaata                                                                                     <  7:462728‑M2/149‑62 (MQ=255)
                                                                  gctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACt                                                                                         <  7:3647‑M2/62‑1 (MQ=255)
                                                                  gctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACaa                                                                                    <  4:360554‑M2/67‑1 (MQ=255)
                                                                     aGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactag                                                                                 <  5:342989‑M2/149‑66 (MQ=255)
                                                                     aGATAGCACAGTGACCGTTGAACTATTCGTACTACATTAATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatacta                                                                                  >  8:218333‑M2/1‑84 (MQ=255)
                                                                      gcaacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAAc                                                                                 <  3:155333‑M2/70‑1 (MQ=255)
                                                                        cacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGt                                                                              <  3:337584‑M2/73‑1 (MQ=255)
                                                                         acgggaatatctgcggcagcgggcttgtaattggttaagtgataacagatgtctggaaatatagtggcaaatacaGTGTATCCGGAGAGGATCATCTGAACTGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGtt                                                                             <  4:123448‑M2/74‑1 (MQ=255)
                                                                         acgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGtt                                                                             <  7:180625‑M2/74‑1 (MQ=255)
                                                                         acgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGtt                                                                             <  2:213832‑M2/74‑1 (MQ=255)
                                                                          cgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGtt                                                                             <  8:30383‑M2/74‑1 (MQ=255)
                                                                          cgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGtt                                                                             >  7:30383‑M2/75‑148 (MQ=255)
                                                                          cgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGt                                                                              >  1:112389‑M2/75‑147 (MQ=255)
                                                                           cacaGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagttttta                                                                           >  7:359388‑M2/1‑78 (MQ=255)
                                                                           cacaGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggcccccagaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagttttta                                                                           >  2:101091‑M2/1‑78 (MQ=255)
                                                                             gaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggaaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCGGCTCATTGCATTTACTGACAAAACCGTTAgcc                                                                         >  2:453111‑M2/72‑148 (MQ=255)
                                                                             caGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttaga                                                                         <  5:25115‑M2/149‑74 (MQ=255)
                                                                              aGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagac                                                                        >  7:388413‑M2/1‑75 (MQ=255)
                                                                                tGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagacta                                                                      >  1:460564‑M2/1‑73 (MQ=255)
                                                                                  aCCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagt                                                                    >  7:447867‑M2/1‑71 (MQ=255)
                                                                                   ctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATAATTGCATTTACTGACAAAACCGTTAGCACCCGCt                                                                   >  2:388531‑M2/66‑149 (MQ=255)
                                                                                     gTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtaga                                                                                                                                              <  4:358128‑M2/72‑5 (MQ=255)
                                                                                     gTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtaga                                                                                                                                              >  3:352199‑M2/1‑68 (MQ=255)
                                                                                        gAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattg                                                               >  1:246299‑M2/1‑65 (MQ=255)
                                                                                        gAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattg                                                               <  5:78877‑M2/148‑84 (MQ=255)
                                                                                         cagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATc                                                             <  1:339197‑M2/90‑1 (MQ=255)
                                                                                         cagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATACTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATc                                                             >  1:323284‑M2/60‑149 (MQ=255)
                                                                                         aaCTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattgg                                                              >  4:201016‑M2/1‑64 (MQ=255)
                                                                                             ggccttgtaatgggttaagtgataacagatgtctggaaatatagtggcaaatccaTTGTATCCGTAGAGTATCATCTGAACCGCATGAAAAATATAAATCTCATCATTGCATTAACTGACAAAACCGTTAGCAAAAGCTTGTATCgcta                                                         >  5:402219‑M2/56‑148 (MQ=255)
                                                                                                 ttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGAc                                                                                      <  6:242455‑M2/65‑1 (MQ=255)
                                                                                                 gTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtt                                                                               <  1:257919‑M2/123‑68 (MQ=255)
                                                                                                 gTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtt                                                                               >  2:257919‑M2/1‑56 (MQ=255)
                                                                                                 gTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaa                                                                                       <  3:50139‑M2/115‑60 (MQ=255)
                                                                                                 gTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaa                                                                                       >  4:50139‑M2/1‑56 (MQ=255)
                                                                                                    ctacATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgat                                                  <  3:81924‑M2/149‑97 (MQ=255)
                                                                                                         tttATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaac                                                         >  6:186633‑M2/1‑48 (MQ=255)
                                                                                                         tttATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaac                                                         <  5:186633‑M2/137‑90 (MQ=255)
                                                                                                          gttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATaaaa                                            <  6:377509‑M2/107‑1 (MQ=255)
                                                                                                           ttaagtgataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAg                                           >  8:432070‑M2/42‑149 (MQ=255)
                                                                                                                 tttttATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttaggg                                     <  1:17400‑M2/149‑110 (MQ=255)
                                                                                                                 gataacagatgtctggaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGa                                                                                       >  3:427655‑M2/36‑99 (MQ=255)
                                                                                                                   tttATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttaggg                                     >  3:241702‑M2/1‑38 (MQ=255)
                                                                                                                       tataGTTAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttagggccg                                  <  1:405305‑M2/146‑113 (MQ=255)
                                                                                                                            ttAAGCGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagagtagtcattggagaacagatgattgatgtcttagggccggagaaacg                          <  4:85406‑M2/149‑121 (MQ=255)
                                                                                                                                gaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCAtt                                                                                                  >  1:125083‑M2/21‑73 (MQ=255)
                                                                                                                                gaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCAtt                                                                                                  <  2:125083‑M2/53‑1 (MQ=255)
                                                                                                                                gaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATaaa                      <  2:195168‑M2/129‑1 (MQ=255)
                                                                                                                                gaaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATaa                       >  4:227307‑M2/21‑148 (MQ=255)
                                                                                                                                 cGTTTCGCTGGGAAAAAACGTGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtct                                                                                          >  2:408866‑M2/1‑24 (MQ=255)
                                                                                                                                 cGTATCGCTGGGAAAAAACGTGTAtagactggccccatgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattgtagaacagatgattgatgtcttagggccggagaaacgcagac                     >  4:258529‑M2/1‑24 (MQ=255)
                                                                                                                                 aaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGc                                                      <  1:409439‑M2/97‑1 (MQ=255)
                                                                                                                                 aaatataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATaaa                      >  6:32380‑M2/20‑148 (MQ=255)
                                                                                                                                  aatataggggcaaatccaGTGTATCCGGAGAGGATCATATGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATaaaaa                    >  2:291298‑M2/19‑149 (MQ=255)
                                                                                                                                     ataggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATaa                 >  3:299989‑M2/16‑149 (MQ=255)
                                                                                                                                      tcggggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGTCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCCGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATaa                 <  8:454647‑M2/134‑1 (MQ=255)
                                                                                                                                      ctctgTGAAATAACGTGTAtagtctggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgatttatgtcttagggccggagaaacgcagacggcg                 >  5:218462‑M2/3‑19 (MQ=255)
                                                                                                                                      cGCTGGGAAAAAACGTGTAtagactcgccccctgaatctccagacaaccaatatcacttaaataagtgatagtctaaatactagttttaagactagtcattggagaacagatgaatgatgtcttagggacggagaaacgcagacggcg                 <  4:97804‑M2/148‑130 (MQ=255)
                                                                                                                                         gggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCtt                                                                  >  8:40542‑M2/12‑96 (MQ=255)
                                                                                                                                         gggcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACt             >  4:325503‑M2/12‑149 (MQ=255)
                                                                                                                                           gcaaatccaGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAGCATTGGGCTTTAGGTGAtaaaaataaaccgttg          >  1:14883‑M2/10‑142 (MQ=255)
                                                                                                                                                  aCATGTAtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttagggccggagaaacgcagacggcgtaccacacagga     <  1:351794‑M2/148‑142 (MQ=255)
                                                                                                                                                    gtgtAtagactggcaccctgaatctccagacaaccaatatcactttaataagtgatagtcttaatactagtttttagactaggcattggagaacagatgattgatgtcttagggccggagaaacgcagacggcgtaccacacaggaaaa  >  3:97388‑M2/1‑5 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TAACTTATCGGCAATTTCTTTATTAGATAATCCGCTAACCAGATAACGTAATATGGTCACTTCACGATTAGATAGCACAGTGACCGTTGAACTATTCGTACTACATTTATTGCTTTTTATATAGTTAAGCGTTTCGCTGGGAAAAAACGTGTATCCGGAGAGGATCATCTGAACGGCATGAAAAATATCATTCTGATCATTGCATTTACTGACAAAACCGTTAGCACCAGCTTGTATCGCTCTGCCAGCATAAAAGCATTCTGATTTCGATGATAAAAATAACACTTTCACTGTGCT  >  NC_000913/563959‑564255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.