Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,218,506 T→A 5.3% I191F (ATC→TTC)  aer ← fused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,218,5060TA 5.3% 238.3 / 3.6 76I191F (ATC→TTC) aerfused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component
Reads supporting (aligned to +/- strand):  ref base T (35/36);  new base A (2/2);  total (37/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01

CCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATA  >  NC_000913/3218358‑3218654
                                                                                                                                                    |                                                                                                                                                    
cccAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGat                                                                                                                                                      <  6:193621/149‑1 (MQ=255)
  cAGCTCATCGCTGCGATTCTGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTACAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCACAACCGGCGCTTGCCAACAATACCCCTAACGCACAGAGGata                                                                                                                                                     >  5:287967/1‑148 (MQ=255)
   aGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAACCAGGCGCTTGCCAACAATACCACTAACGCACAGAGGAtata                                                                                                                                                   >  6:233603/1‑149 (MQ=255)
        aTCGCTGCGATTCTGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTc                                                                                                                                               <  2:51861/148‑1 (MQ=255)
        aTCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGt                                                                                                                                                <  4:117781/147‑1 (MQ=255)
                gATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCATTGCCTTATGTGCAACATTTTCTATCTGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCgg                                                                                                                                       <  8:165721/148‑1 (MQ=255)
                 aTTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCgggg                                                                                                                                     >  2:192418/1‑149 (MQ=255)
                       aTGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACCATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGcaa                                                                                                                               <  1:207790/149‑1 (MQ=255)
                           tCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACaaa                                                                                                                           >  2:298147/1‑149 (MQ=255)
                               cacTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCa                                                                                                                        >  1:132943/1‑148 (MQ=255)
                                    ttACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCa                                                                                                                   <  8:15384/148‑1 (MQ=255)
                                    ttACGTTCTCCGGTCGCCACCTCCAGTGCCTGATGGTCAACATTTTCTATCGGGCTCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCATAGCAt                                                                                                                  <  2:329186/149‑1 (MQ=255)
                                     tACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCCACAATACCACGAACGCACAGAGGATATACGTCCCCGCCGGGGCAGCAACAAACCAAAGCATg                                                                                                                 >  2:166239/1‑149 (MQ=255)
                                      aCGTTCTCCGGTCGCCACCTTCAGTGGCTGATGTGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATg                                                                                                                 <  2:164404/148‑1 (MQ=255)
                                      aCGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATgg                                                                                                                >  4:257319/1‑149 (MQ=255)
                                      aCGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATg                                                                                                                 <  3:62616/148‑1 (MQ=255)
                                          tctcCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGgccgc                                                                                                            >  3:38906/1‑149 (MQ=255)
                                              cGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCa                                                                                                          <  1:192418/147‑1 (MQ=255)
                                              cGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCa                                                                                                          <  5:338363/147‑1 (MQ=255)
                                               ggTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGc                                                                                                        >  3:182045/1‑148 (MQ=255)
                                                gTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACCGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGGCCCCACCGGGGCAGCAGCAACCCAAAGCATGGCCGCCAGc                                                                                                        >  2:45330/1‑147 (MQ=255)
                                                    ccACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCTATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATa                                                                                                  <  7:267439/149‑1 (MQ=255)
                                                     cACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATaa                                                                                                 <  2:132943/149‑1 (MQ=255)
                                                     cACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATaa                                                                                                 <  7:173245/149‑1 (MQ=255)
                                                     cACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATa                                                                                                  >  1:214976/1‑148 (MQ=255)
                                                           cAGTGCCTGATGGTCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACAt                                                                                             <  7:307414/147‑1 (MQ=255)
                                                            aGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCa                                                                                           <  4:38906/148‑1 (MQ=255)
                                                             gTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAgg                                                                                         <  3:257319/149‑1 (MQ=255)
                                                             gTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCACTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAgg                                                                                         >  3:215159/1‑149 (MQ=255)
                                                                ccTGATGGGCAACATTTTCTATCGGGCGCACCATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCCCCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGt                                                                                       >  1:72099/1‑148 (MQ=255)
                                                                ccTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAGTACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGt                                                                                       <  2:211465/148‑1 (MQ=255)
                                                                  tGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAAc                                                                                               >  1:334049/1‑138 (MQ=255)
                                                                  tGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAAc                                                                                               <  2:334049/138‑1 (MQ=255)
                                                                  tGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGAAATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGtcat                                                                                    >  8:216009/1‑149 (MQ=255)
                                                                  tGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGAAATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGtcat                                                                                    >  8:211064/1‑149 (MQ=255)
                                                                        gCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTc                                                                               >  3:170805/1‑148 (MQ=255)
                                                                         cAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCa                                                                             >  1:180137/1‑149 (MQ=255)
                                                                         cAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCa                                                                             >  3:181897/1‑149 (MQ=255)
                                                                           aCATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCAcg                                                                           >  7:342850/1‑149 (MQ=255)
                                                                            cATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCAcgc                                                                          >  5:290886/1‑149 (MQ=255)
                                                                             aTTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCAcgcg                                                                         >  2:108446/1‑149 (MQ=255)
                                                                                  cTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGCGGATATACGTCACCCCCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGGCATCCCTCCCCGcgcccgc                                                                    >  6:234720/1‑149 (MQ=255)
                                                                                  cTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGcgcccgc                                                                    >  4:131616/1‑149 (MQ=255)
                                                                                   tATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGcgcccgc                                                                    >  3:219435/1‑148 (MQ=255)
                                                                                        ggCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATCCCACTAACGCACAGCGGATATACGACCCCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGAAAGATAAACATCAGGGTCAACCCTCCACGCGCCCGCCAGcc                                                               >  2:245763/1‑147 (MQ=255)
                                                                                        ggCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCg                                                               >  4:125945/1‑148 (MQ=255)
                                                                                        ggCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGac                                                             >  4:310790/1‑148 (MQ=255)
                                                                                          cgcACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAg                                                            <  7:179257/149‑1 (MQ=255)
                                                                                           ggacaATCTGCCATTCAAAACATGCGCTTTCCAACAATACCACTAACGCACAGAGGATATACGTCACCACAGGGGAAGCAACAAACCCAAGCATGGCCGCCAGCAAGATAAACATCATGGTCATCACTCCACGCGACCGCCAGAGAAGc                                                           <  4:241550/147‑1 (MQ=255)
                                                                                            cacaATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCg                                                          >  4:232165/1‑149 (MQ=255)
                                                                                             acaATCTGCCATTCAAAACAGGCACTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCgg                                                         <  4:215159/149‑1 (MQ=255)
                                                                                                     ccATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAg                                                 >  1:339882/1‑149 (MQ=255)
                                                                                                       aTTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCCCCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAgg                                                >  1:205777/1‑148 (MQ=255)
                                                                                                          cAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAg                                             <  8:342850/148‑1 (MQ=255)
                                                                                                           aaaaCAGGCGCTTGCCAACAATACCACTAACGCACAGAGTATATACGTAACCACCGGGGAAGCAACAAACCAAAGCATGGCCTCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGt                                            <  1:107949/148‑1 (MQ=255)
                                                                                                               cAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCTAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTAcc                                       <  4:182045/149‑1 (MQ=255)
                                                                                                               cAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAAAAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTAcc                                       <  2:180137/149‑1 (MQ=255)
                                                                                                                     cTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACccagcca                                 <  6:290886/149‑1 (MQ=255)
                                                                                                                         ccAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCagccagcc                              <  4:181897/148‑1 (MQ=255)
                                                                                                                         ccAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCt                             <  2:214976/149‑1 (MQ=255)
                                                                                                                           aacaaTACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCttt                           <  1:108446/149‑1 (MQ=255)
                                                                                                                           aacaaTACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCtt                            >  8:106917/1‑148 (MQ=255)
                                                                                                                           aacaaTACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCtt                            >  4:281902/1‑148 (MQ=255)
                                                                                                                             caaTACCACTAACGCTCAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTAc                         <  2:225017/149‑1 (MQ=255)
                                                                                                                                  ccACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGcac                     <  5:254976/148‑1 (MQ=255)
                                                                                                                                   cACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGcacca                   >  1:17943/1‑149 (MQ=255)
                                                                                                                                     cTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCg                                                          >  2:66280/1‑108 (MQ=255)
                                                                                                                                     cTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCg                                                          <  1:66280/108‑1 (MQ=255)
                                                                                                                                      tAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGcaccac                  >  2:340939/1‑147 (MQ=255)
                                                                                                                                        aCGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAgg              >  7:37853/1‑149 (MQ=255)
                                                                                                                                        aCGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAgg              >  1:77870/1‑149 (MQ=255)
                                                                                                                                        aCGCACAGAGGATATACGGCACCCCCGGGGCAGCAACAAACCACAGCATGGCCGCCAGCAAGCTAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAgg              <  6:287967/149‑1 (MQ=255)
                                                                                                                                             caGAGGATATACGTCACCACCGGTGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCttt         <  7:262545/149‑1 (MQ=255)
                                                                                                                                              agagGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCTCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTa        <  6:206015/149‑1 (MQ=255)
                                                                                                                                              agagGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGGTTACCCAGCCAGCCTTTACGCACCACCAGGCCTGTa        >  4:246355/1‑149 (MQ=255)
                                                                                                                                              agagGAGATACGTCACCACCGGGGCAGCAACAAACCAAAGCAGGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTa        <  2:72099/149‑1 (MQ=255)
                                                                                                                                               gagGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTAt       <  1:340939/149‑1 (MQ=255)
                                                                                                                                                 ggAAATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGa     <  7:216009/149‑1 (MQ=255)
                                                                                                                                                 ggAAATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGa     <  7:211064/149‑1 (MQ=255)
                                                                                                                                                   atatACGTCACCACCGGGGCAGCAACAAACCACAGCATGGCCGCCAGCAAGAGAAACAGCAGGGTCATCACTCCACGCGCCCGCCAGCGCAGCGGTAATGTAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAAt   <  6:243800/149‑1 (MQ=255)
                                                                                                                                                    tataCGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATa  <  3:232165/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACAGAGGATATACGTCACCACCGGGGCAGCAACAAACCAAAGCATGGCCGCCAGCAAGATAAACATCAGGGTCATCACTCCACGCGCCCGCCAGCGAAGCGGTAATGAAGGCAGTTTACCCAGCCAGCCTTTACGCACCACCAGGCCTTTATGAATA  >  NC_000913/3218358‑3218654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: