Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,683,207 A→G 6.6% A115A (GCA→GCG)  yphH → putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,683,2070AG 6.6% 177.9 / 2.7 63A115A (GCA→GCG) yphHputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (33/23);  new base G (2/2);  total (37/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

GCATAGTAGCGGTACATGGCTGATTGCGCCGGAAGGTGACTGGACGCTGTGCCTGAACGTGACGCCCACCAGTATTGAGTGTCAGGTTGCTAATGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAACCTGGCGTTGGCAATCCACGGTCAGGTTGATCCTGTGACCGGCGTGTCGCAAACCATG  >  NC_000913/2683066‑2683345
                                                                                                                                             |                                                                                                                                          
gCATAGTAGCGGTACATGGCTGATTGCGCCGGAAGGTGACTGGACGCTGTGCCTGAACGTGACGCCCACCAGTATTGAGTGTCAGGTTGCTAATGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGAcgc                                                                                                                                     >  1:291478/1‑149 (MQ=255)
   tagtagCGGTACATGGCTGATTGCGCCGGAAGGTGACTGGACGCTGTGCCTGAACGTGACGCCCACCAGTATTGAGTGTCAGGTTGCTAATGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGAcgccg                                                                                                                                   >  2:221869/1‑148 (MQ=255)
     gtagCGGTACATGGCTGATTGCGCCGGAAGGTGACTGGACGCTGTGCCTGAACGTGACGCCCACCAGTATTGAGTGTCAGGTTGCTAATGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAg                                                                                                                                >  3:139686/1‑149 (MQ=255)
                 ggCTGATTGCGCCGGAAGGTGACTGGACGCTGTGCCTGAACGTGACGCCCACCAGTATTGAGTGTCAGGTTGCTAATGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTcc                                                                                                                    >  1:198223/1‑149 (MQ=255)
                  gCTGAGTGCGGCGGACGGGGCCGGGCCGCGGTGCCGGACCGGGACGCCCCCCCGTCGGGCGGGGCAGGGTGCTACTGCTTGTGTAAGGCCGACAGGTGACTGTGAGTAGTTACAGATTGCTGCCCCGACGCCGCAGGCGCTGCTGGcc                                                                                                                    <  1:142382/148‑1 (MQ=255)
                           gcCGGAAGGTGACTGGACGCTGTGCCTGAACGTGACGCCCACCAGTATTGAGTGTCAGGTTGCTAATGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGaaa                                                                                                          <  8:240706/149‑1 (MQ=255)
                            ccGGAAGGTGACTGGACGCTGTGCCTGAACGTGACGCCCACCAGTATTGAGTGTCAGGTTGCTAATGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGaaa                                                                                                          >  8:332216/1‑148 (MQ=255)
                              ggAAGGTGACTGGACGCTGTGCCTGAACGTGACGCCCACCAGTATTGAGTGTCAGGTTGCTAATGCTTGTTTAAGTCCGATAGGTGAATTTGAGTATTTACAGATTGATGCTCCGACGCCGCAGGGGCTGCTGTCCGAAATCGAAAAAt                                                                                                       >  4:201307/1‑149 (MQ=255)
                               gAAGGTGACTGGACGCTGTGCCTGAACGTGACGCCCACCAGTATTGAGTGTCAGGTTGCTAATGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGAACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATg                                                                                                      >  3:47106/1‑149 (MQ=255)
                                  ggTGACTGGACGCTGTGCCTGAACGTGACGCCCACCAGTATTGAGTGTCAGGTTGCTAATGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTg                                                                                                   <  2:198223/149‑1 (MQ=255)
                                        tGGACGCTGTGCCTGAACGTGACGCCCACCAGTATTGAGTGTCAGGTTGCTAATGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCg                                                                                             >  1:50747/1‑149 (MQ=255)
                                          gccGCAGTGCCTGCACGTGACGCCCACCAGAATTGAGTGTCAGGTTGCTAATGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATATACAGATTGATGCACCGACGCCGCAGGCGCTCCTGTCCGAAATCGAACAATTCTGGCATCTcc                                                                                           >  4:95296/3‑149 (MQ=255)
                                          gACGCTGTGCCTGAACGTGACGCCCACCAGTATTGAGTGTCAGGTTGCTAATGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGcc                                                                                           <  7:332216/149‑1 (MQ=255)
                                           aCGCTGTGCCTGAACGTGACGCCCACCAGTATTGAGTTTCAGGTTGCTAATGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCa                                                                                          <  5:304079/149‑1 (MQ=255)
                                           aCGCTGTGCCTGAACGTGACGCCCACCAGTATTGAGTGTCAGGTTTCTAATGCTTGTTTAAGTCCGAAAGGGGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCa                                                                                          <  3:123068/149‑1 (MQ=255)
                                                    cTGAACGTGACGCCCACCAGTATTGAGTGTCAGGTTGCTAATGCTTGTTTAAGTCCGATAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAAtt                                                                                 >  2:265817/1‑149 (MQ=255)
                                                     tGAACGTGACGCCCACCAGTATTGAGTGTCAGGTTGCTAATGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAAtt                                                                                 >  1:97303/1‑148 (MQ=255)
                                                      gAACGTGACGCCCACCAGTATTGAGTGTCAGGTTGCTAATGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCGCCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATtgt                                                                               >  8:293645/1‑149 (MQ=255)
                                                       aaCGTGACGCCCACCAGTATTGAGTGTCAGGTTGCTAATGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAAtt                                                                                 <  2:97303/146‑1 (MQ=255)
                                                       aaCGTGACGCCCACCAGTATTGAGTGTCAGGTTGCTAATGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATtgt                                                                               <  7:268219/148‑1 (MQ=255)
                                                              cGCCCACCAGTATTGAGTGTCAGGTTGCTAATGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCGCCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGac                                                                       <  7:293645/149‑1 (MQ=255)
                                                              cGCCCACCAGTATTGAGTGTCAGGTTGCTAATGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGac                                                                       <  4:47106/149‑1 (MQ=255)
                                                                 ccaccaGTATTGAGTGTCAGGTTGCTAATGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGacca                                                                     >  1:119076/1‑148 (MQ=255)
                                                                  caccaGTATTGAGTGTCAGGTTGCTAATGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATtgtg                                                                              <  2:186965/138‑1 (MQ=255)
                                                                  caccaGTATTGAGTGTCAGGTTGCTAATGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATtgtg                                                                              >  1:186965/1‑138 (MQ=255)
                                                                  caccaGTATTGAGTGTCAGGTTGCTAATGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGaccata                                                                   >  2:336410/1‑149 (MQ=255)
                                                                   accaGTATTGAGTGTCAGGTTGCTAATGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGaccatac                                                                  >  7:101132/1‑149 (MQ=255)
                                                                     caGTATTGAGTGTCAGGTTGCTAATGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAg                                                                                                                                >  7:318375/1‑85 (MQ=255)
                                                                     caGTATTGAGTGTCAGGTTGCTAATGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAg                                                                                                                                <  8:318375/85‑1 (MQ=255)
                                                                             aGTGTCAGGTTGCTAATGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAACCTg                                                        >  6:753/1‑149 (MQ=255)
                                                                                 tCAGGTTGCTAATGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAACCTGGCGt                                                    <  7:42723/149‑1 (MQ=255)
                                                                                 tCAGGTTGCTAATGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAACCTGGCGt                                                    >  6:28017/1‑149 (MQ=255)
                                                                                  cAGGTTGCTAATGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAACCTGGCGtt                                                   >  6:125278/1‑149 (MQ=255)
                                                                                   aGGTTGCTAATGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAACCTGGCGTTg                                                  <  7:183481/149‑1 (MQ=255)
                                                                                         cTAATGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAACCTGGCGTTGGCAATc                                            >  8:86774/1‑149 (MQ=255)
                                                                                          tAATGCTTGTTTAAGTCCGAAAGCTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCTGACCATACCATCAACCTGGCGTTGGCAATcc                                           >  1:248293/1‑149 (MQ=255)
                                                                                            aTGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAACCTGGCGTTGGCAATCCAc                                         >  8:319745/1‑149 (MQ=255)
                                                                                            aTGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAACCTGGCGTTGGCAATCCAc                                         >  7:95346/1‑149 (MQ=255)
                                                                                             tGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAACCTGGCGTTGGCAATCCACg                                        >  3:147262/1‑149 (MQ=255)
                                                                                              gCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAACCTGGCGTTGGCAATCCACgg                                       >  3:250786/1‑149 (MQ=255)
                                                                                                   tttAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCGCCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAACCTGGCGTTGGCAATCCACGGTCAg                                   >  5:182772/1‑148 (MQ=255)
                                                                                                   tttAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCGCCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAACCTGGCGTTGGCAATCCACGGTCAg                                   <  6:182772/148‑1 (MQ=255)
                                                                                                   tttAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCCCCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAACCTGGCGTTGGCAAGCCACGGTCAgg                                  >  3:48708/1‑149 (MQ=255)
                                                                                                   tttAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAACCTGGCGTTGGCAATCCACGGTCAgg                                  >  7:225821/1‑149 (MQ=255)
                                                                                                     tAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAACCTGGCGTTGGCAATCCACGGTCAGGt                                 <  2:50747/148‑1 (MQ=255)
                                                                                                     tAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAACCTGGCGTTGGCAATCCACGGTCAGGt                                 <  7:86774/148‑1 (MQ=255)
                                                                                                     tAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAACCTGGCGTTGGCAATCCACGGTCAGGt                                 <  4:250786/148‑1 (MQ=255)
                                                                                                       aGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAACCTGGCGTTGGCAATCCACGGTCAGGTTg                               <  1:336410/148‑1 (MQ=255)
                                                                                                        gTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAACCTGGCGTTGGCAATCCACGGTCAGGTTGAt                             <  5:125278/149‑1 (MQ=255)
                                                                                                         tCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAACCTGGCGTTGGCAATCCACGGTCAGGTTGATc                            >  2:188907/1‑149 (MQ=255)
                                                                                                         tCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAACCTGGCGTTGGCAATCCACGGTCAGGTTGATc                            <  2:119076/149‑1 (MQ=255)
                                                                                                           cGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAAcc                                                          <  8:268673/117‑1 (MQ=255)
                                                                                                           cGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAAcc                                                          >  7:268673/1‑117 (MQ=255)
                                                                                                            gAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAACCTGGCGTTGGCAATCCACGGTCAGGTTGATCCtg                         >  8:19361/1‑149 (MQ=255)
                                                                                                             aaaGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAACCTGGCGTTGGCAATCCACGGTCAGGTTGATCCtgt                        <  5:28017/149‑1 (MQ=255)
                                                                                                                    aaTTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGACAGCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAACCTGGCGTTGGCAATCCACGGTCAGGTTGATCCTGTGACCGGc                 <  2:291478/149‑1 (MQ=255)
                                                                                                                    aaTTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAACCTGGCGTTGGCAATCCACGGTCAGGTTGATCCTGTGACCGGc                 <  1:265817/149‑1 (MQ=255)
                                                                                                                               ttACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAACCTGGCGTTGGCAATCCACGGTCAGGTTGATCCTGTGACCGGCGTGTCGCAAAc      >  3:102118/1‑149 (MQ=255)
                                                                                                                               ttACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAACCTGGCGTTGGCAATCCACGGTCAGGTTGATCCTGTGACCGGCGTGTCGCAAAc      >  7:230827/1‑149 (MQ=255)
                                                                                                                                tACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAACCTGGCGTTGGCAATCCACGGTCAGGTTGATCCTGTGACCGGCGTGTCGCAAAc      >  7:172977/1‑148 (MQ=255)
                                                                                                                                 aCAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAACCTGGCGTTGGCAATCCACGGTCAGGTTGATCCTGTGACCGGCGTGTCg           <  6:3673/142‑1 (MQ=255)
                                                                                                                                 aCAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAACCTGGCGTTGGCAATCCACGGTCAGGTTGATCCTGTGACCGGCGTGTCg           >  5:3673/1‑142 (MQ=255)
                                                                                                                                   aGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAACCTGGCGTTGGCAATCCACGGTCAGGTTGATCCTGTGACCGGCGTGTCGCAAACCATg  >  4:317227/1‑149 (MQ=255)
                                                                                                                                             aCCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAACCTGGCGTTGGCAATCCACGGTCAgg                                  >  6:276204/1‑107 (MQ=255)
                                                                                                                                             aCCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAACCTGGCGTTGGCAATCCACGGTCAgg                                  <  5:276204/107‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                          
GCATAGTAGCGGTACATGGCTGATTGCGCCGGAAGGTGACTGGACGCTGTGCCTGAACGTGACGCCCACCAGTATTGAGTGTCAGGTTGCTAATGCTTGTTTAAGTCCGAAAGGTGAATTTGAGTATTTACAGATTGATGCACCGACGCCGCAGGCGCTGCTGTCCGAAATCGAAAAATGCTGGCATCGCCACCGTAAATTGTGGCCGGACCATACCATCAACCTGGCGTTGGCAATCCACGGTCAGGTTGATCCTGTGACCGGCGTGTCGCAAACCATG  >  NC_000913/2683066‑2683345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑