Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,601,805 A→G 6.6% Q202R (CAG→CGG)  hyfA → hydrogenase 4, 4Fe‑4S subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,601,8050AG 6.6% 229.1 / 5.1 77Q202R (CAG→CGG) hyfAhydrogenase 4, 4Fe‑4S subunit
Reads supporting (aligned to +/- strand):  ref base A (31/39);  new base G (2/3);  total (34/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

CCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATT  >  NC_000913/2601657‑2601952
                                                                                                                                                    |                                                                                                                                                   
ccAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCctcactca                                                                                                                                                     <  8:33185/149‑1 (MQ=255)
 cAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAg                                                                                                                                                    <  7:7592/149‑1 (MQ=255)
   gAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAg                                                                                                                                                    >  8:51747/1‑147 (MQ=255)
   gAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGa                                                                                                                                                  <  8:94667/149‑1 (MQ=255)
   gAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCATGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGa                                                                                                                                                  <  3:117160/149‑1 (MQ=255)
       gTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCaa                                                                                                                                              >  2:142738/1‑149 (MQ=255)
          cGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGc                                                                                                                                           <  8:42032/149‑1 (MQ=255)
          cGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGc                                                                                                                                           <  7:51747/149‑1 (MQ=255)
            gCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCGGGAGCAACGCt                                                                                                                                          >  6:69732/1‑148 (MQ=255)
              cTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAAt                                                                                                                                       <  7:193398/149‑1 (MQ=255)
                  gTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGAt                                                                                                                                   >  6:290848/1‑149 (MQ=255)
                             cacGAATCAGGCGTTAAGGCTGATCACCGGTGATATCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAatt                                                                                                                        <  1:332625/147‑1 (MQ=255)
                               cGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCGGGAGCAACGCTAATGGATGCCCTGCAattat                                                                                                                      >  8:155358/1‑149 (MQ=255)
                               cGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAattat                                                                                                                      >  7:265230/1‑149 (MQ=255)
                               cGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACCTCAGATGAAAGAAAAACACCGCCTTGCCGCAAGCTGGTTTGCCATTGGCGGGGAGGATCCCCTTTCCCTCCCTCCGGAGCAACGCTAATGGATGCCCTGCAattat                                                                                                                      >  8:160162/1‑149 (MQ=255)
                                gAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAACACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAagtat                                                                                                                      >  5:137945/1‑148 (MQ=255)
                                  aTCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTaa                                                                                                                   <  4:15082/149‑1 (MQ=255)
                                      ggCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTg                                                                                                               <  3:147642/149‑1 (MQ=255)
                                      ggCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTg                                                                                                               <  1:143028/149‑1 (MQ=255)
                                        cGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAAt                                                                                                                                       <  2:84868/123‑1 (MQ=255)
                                        cGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAAt                                                                                                                                       >  1:84868/1‑123 (MQ=255)
                                            aCGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGgcgc                                                                                                          <  8:265230/148‑1 (MQ=255)
                                                cTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGAtt                                                                                                     <  4:128788/149‑1 (MQ=255)
                                                   aTCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATtctc                                                                                                  >  5:221483/1‑149 (MQ=255)
                                                     cACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCCCTCAGGAGCAACGCTAATGGATGCCCTGAAATTATTAACCTGGTCGCTGATTCTCTa                                                                                                >  7:306438/1‑149 (MQ=255)
                                                      aCCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTAt                                                                                               <  5:73248/149‑1 (MQ=255)
                                                       ccGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATc                                                                                              >  1:282333/1‑149 (MQ=255)
                                                        cGGTGATAGCCTGCAACGTCAGATGACAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGAGCCCCTTTCCCTCACTCGGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCt                                                                                             <  7:160162/149‑1 (MQ=255)
                                                        cGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCGGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCt                                                                                             <  7:155358/149‑1 (MQ=255)
                                                          gTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCCGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGt                                                                                           >  7:313404/1‑149 (MQ=255)
                                                            gATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGttt                                                                                         <  6:45044/149‑1 (MQ=255)
                                                            gATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGttt                                                                                         <  4:140958/149‑1 (MQ=255)
                                                             aTAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGttt                                                                                         <  2:181817/148‑1 (MQ=255)
                                                              tAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAgg                                                                                                                                                   >  3:302907/1‑89 (MQ=255)
                                                              tAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAgg                                                                                                                                                   <  4:302907/89‑1 (MQ=255)
                                                                   tGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTGGCTGATTCTCTATCTGTTTGCTAGTc                                                                                  >  7:250653/1‑149 (MQ=255)
                                                                   tGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTc                                                                                  >  6:123094/1‑149 (MQ=255)
                                                                     cAACGTCAGATGTAAGATAAACAGCGCCTTGCCTCAAGCTGGTTTGCCAATGGCGGGTAGGATACCCTTTCTCTCACTCAGGAGCACCTCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCt                                                                                 <  8:313404/148‑1 (MQ=255)
                                                                       aCGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGc                                                                              >  4:9427/1‑149 (MQ=255)
                                                                           cAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATGAACCTGGTCGCGGATTCTCGATCTGTTTGCTAGTCTGGCTTCg                                                                          >  6:172811/1‑149 (MQ=255)
                                                                            aGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCg                                                                          <  5:290848/148‑1 (MQ=255)
                                                                                 aaagaaaAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCACCGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGATGttt                                                                    >  7:297009/1‑149 (MQ=255)
                                                                                 aaagaaaAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGttt                                                                    <  8:28114/149‑1 (MQ=255)
                                                                                 aaagaaaAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGttt                                                                    <  2:283670/149‑1 (MQ=255)
                                                                                 aaagaaaAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGttt                                                                    <  4:292983/149‑1 (MQ=255)
                                                                                 aaagaaaAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGtt                                                                     <  6:131158/148‑1 (MQ=255)
                                                                                  aagaaaAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGtttt                                                                   >  3:70163/1‑149 (MQ=255)
                                                                                   agaaaAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGtttt                                                                   >  7:176950/1‑148 (MQ=255)
                                                                                    gaaaAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTa                                                                 >  6:224163/1‑149 (MQ=255)
                                                                                     aaaaaCAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTAc                                                                >  8:194550/1‑149 (MQ=255)
                                                                                     aaaaaCAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTAc                                                                >  8:6380/1‑149 (MQ=255)
                                                                                       aaaCAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTc                                                              <  4:70163/149‑1 (MQ=255)
                                                                                        aaCAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCg                                                             <  4:269650/149‑1 (MQ=255)
                                                                                             cgcCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTg                                                        <  3:9427/149‑1 (MQ=255)
                                                                                             cgcCGTGCCGCAAGCTGGTTTGCCAAGGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGGCTGGCTTCGCTGTTTTTACTCGGTCTg                                                        <  4:87157/149‑1 (MQ=255)
                                                                                                      gCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTgg                                                                               >  3:209004/1‑117 (MQ=255)
                                                                                                      gCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTgg                                                                               <  4:209004/117‑1 (MQ=255)
                                                                                                      gCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTg                                               <  7:6380/149‑1 (MQ=255)
                                                                                                         aGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCGGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCt                                            <  5:69732/149‑1 (MQ=255)
                                                                                                            tGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCCCTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTTGTCTGGCTTCGCTGTTTTTACTCGGGCTGGACAGACTGGCTAt                                          >  7:143349/1‑148 (MQ=255)
                                                                                                             ggTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTAtt                                         >  7:263759/1‑148 (MQ=255)
                                                                                                              gTTTGCCAATGGCGGGGAGGATCCCCGTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTaa                                       <  3:205576/149‑1 (MQ=255)
                                                                                                                  gCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCtt                                   >  1:226247/1‑149 (MQ=255)
                                                                                                                   ccAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCttt                                  <  1:174030/149‑1 (MQ=255)
                                                                                                                    cAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGTAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTc                                 >  1:198448/1‑149 (MQ=255)
                                                                                                                    cAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTc                                 >  2:344464/1‑149 (MQ=255)
                                                                                                                     aaTGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGACTCTCTATCTGTTTGCTTGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTcc                                >  1:318812/1‑149 (MQ=255)
                                                                                                                          cGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCAt                           <  8:337775/149‑1 (MQ=255)
                                                                                                                           ggggAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATc                          <  1:142738/149‑1 (MQ=255)
                                                                                                                           ggggAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATc                          <  1:344464/149‑1 (MQ=255)
                                                                                                                             ggaggaTCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTaa                                       <  3:266405/134‑1 (MQ=255)
                                                                                                                             ggaggaTCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTaa                                       >  4:266405/1‑134 (MQ=255)
                                                                                                                                      ccgttCCCTCACTCAGGCGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCTCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGGCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCtggt               <  8:297009/146‑1 (MQ=255)
                                                                                                                                      ccTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATAAAGCTTTCCGGCATCACATCGCtggt               >  3:108280/1‑149 (MQ=255)
                                                                                                                                       cTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTaa                                       <  1:324469/124‑1 (MQ=255)
                                                                                                                                       cTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTaa                                       >  2:324469/1‑124 (MQ=255)
                                                                                                                                       cTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCtggtg              <  4:108280/149‑1 (MQ=255)
                                                                                                                                            cctccctcCGGAGCAAGGCTACTGGACGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGGCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGggcg          <  1:209193/148‑1 (MQ=255)
                                                                                                                                              tcactcaGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTAc                                                                >  2:285093/1‑92 (MQ=255)
                                                                                                                                                  tcaGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGTCATCACATCGCTGGTGGGCGGCGTGAt   >  8:92155/1‑149 (MQ=255)
                                                                                                                                                  tcaGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGAt   >  8:183007/1‑149 (MQ=255)
                                                                                                                                                    aGGCGCAACGCTAATGGAGGCCCTGCAATTATTACCCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGAtt  <  6:137945/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGATCCCCTTTCCCTCACTCAGGAGCAACGCTAATGGATGCCCTGCAATTATTAACCTGGTCGCTGATTCTCTATCTGTTTGCTAGTCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATT  >  NC_000913/2601657‑2601952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑