Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,054,509 G→A 5.0% A257V (GCA→GTA)  glnG ← fused DNA‑binding response regulator in two‑component regulatory system with GlnL: response regulator/sigma54 interaction protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,054,5090GA 5.0% 251.8 / 2.4 80A257V (GCA→GTA) glnGfused DNA‑binding response regulator in two‑component regulatory system with GlnL: response regulator/sigma54 interaction protein
Reads supporting (aligned to +/- strand):  ref base G (44/31);  new base A (2/2);  total (47/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01

CGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGAATAATGTACCGCCATCGGCCTGTTCAAAACGCCCCTGACGAATGGTATTCGCGCCAGTAAACGCGCCTTTCTCGTGGCCAAACA  >  NC_000913/4054363‑4054653
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cgcgGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGcc                                                                                                                                                <  8:111695/149‑1 (MQ=255)
   ggATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAg                                                                                                                                              >  8:345239/1‑148 (MQ=255)
       aaCGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGAGAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCATCTGCCAGCAcgc                                                                                                                                         >  6:169153/1‑149 (MQ=255)
        aCGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCAcgcg                                                                                                                                        <  6:185123/149‑1 (MQ=255)
           ttCAGGCGGTGGAACAGATCCTCACGGAGCTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCgcag                                                                                                                                     >  2:204119/1‑149 (MQ=255)
           ttCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCgcag                                                                                                                                     >  8:8/1‑149 (MQ=255)
              aGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTACCAGCACGCgcagca                                                                                                                                   >  6:120968/1‑148 (MQ=255)
              aGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTACCAGCACGCgcagca                                                                                                                                   >  6:115732/1‑148 (MQ=255)
                  ggTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAAcgc                                                                                                                              <  2:174558/149‑1 (MQ=255)
                   gTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAAcgcg                                                                                                                             >  2:7369/1‑149 (MQ=255)
                     ggAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTc                                                                                                                           >  3:77908/1‑149 (MQ=255)
                      gAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCAAGCACGCGCAGCAAACGCGTCt                                                                                                                          >  6:189300/1‑149 (MQ=255)
                        aCAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTg                                                                                                                         >  5:105365/1‑148 (MQ=255)
                        aCAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGc                                                                                                                        <  7:8/149‑1 (MQ=255)
                            aTCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACAt                                                                                                                    >  1:268246/1‑149 (MQ=255)
                            aTCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATACACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGCCCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACAt                                                                                                                    >  2:134273/1‑149 (MQ=255)
                                tCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAg                                                                                                                >  1:154931/1‑149 (MQ=255)
                                  aCGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGc                                                                                                               <  6:215182/148‑1 (MQ=255)
                                  aCGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGc                                                                                                               <  5:210101/148‑1 (MQ=255)
                                  aCGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCg                                                                                                              >  1:198913/1‑149 (MQ=255)
                                      aaCTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCat                                                                                                          <  2:154931/149‑1 (MQ=255)
                                         ttACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATc                                                                                                       >  3:38682/1‑149 (MQ=255)
                                           aCCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCa                                                                                                      <  2:301929/148‑1 (MQ=255)
                                           aCCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCAc                                                                                                     >  6:50386/1‑149 (MQ=255)
                                            ccTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCAcc                                                                                                    <  4:77908/149‑1 (MQ=255)
                                             cTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCgcagc                                                                                                                                    >  4:278915/1‑116 (MQ=255)
                                             cTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCgcagc                                                                                                                                    <  3:278915/116‑1 (MQ=255)
                                             cTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCAcc                                                                                                    >  1:175677/1‑148 (MQ=255)
                                              ttCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCaa                                                                                                  >  1:40749/1‑149 (MQ=255)
                                              ttCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACATTCACCGGCGCATAGCCGCCAACCCGGTAAAACAGACCGTCTTCCAGCACGCGCACCAAAAGCGACTGCACAACCAGCCGCAAATCACCa                                                                                                   >  8:145737/1‑148 (MQ=255)
                                                ccTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGt                                                                                                                            <  3:282722/121‑1 (MQ=255)
                                                ccTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGt                                                                                                                            >  4:282722/1‑121 (MQ=255)
                                                    cACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCTCCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACTCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTtcg                                                                                             <  7:145737/148‑1 (MQ=255)
                                                    cACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTtcgt                                                                                            >  8:343/1‑149 (MQ=255)
                                                     aCTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTtcgtc                                                                                           <  1:7369/149‑1 (MQ=255)
                                                     aCTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTtcgtc                                                                                           >  1:234604/1‑149 (MQ=255)
                                                       tCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTtcgtc                                                                                           >  2:82639/1‑147 (MQ=255)
                                                         gCTGTTCGAGATTCTGGTGCGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAgg                                                                                       <  3:249309/149‑1 (MQ=255)
                                                         gCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGa                                                                                         <  2:175677/147‑1 (MQ=255)
                                                         gCTGTTCGAGATTCTGGGGAGTGGCAGAGATAATCCGCACATCCTCTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCGAGCACGCGCAGCAAACGCGTCTGCAAATCCAGCGGCATATCACCAATTTCGTCGAgg                                                                                       <  5:218204/149‑1 (MQ=255)
                                                             ttCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGaat                                                                                    >  1:6199/1‑148 (MQ=255)
                                                               cGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGaataat                                                                                 >  7:55357/1‑149 (MQ=255)
                                                               cGAGATTCTGGTGAGTGGCAACGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGaataa                                                                                  <  8:244229/148‑1 (MQ=255)
                                                               cGAGATTCTGGTGAGTGGCAACGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGaataa                                                                                  >  7:244229/1‑148 (MQ=255)
                                                                gagaTTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGaataat                                                                                 <  3:121230/148‑1 (MQ=255)
                                                                gagaTTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGaataat                                                                                 <  1:274008/148‑1 (MQ=255)
                                                                gagaTTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGAATAATg                                                                                >  3:169329/1‑149 (MQ=255)
                                                                 agaTTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGAATAATg                                                                                >  8:211567/1‑148 (MQ=255)
                                                                 agaTTCTGGGGAGTGGCATCGATAATGCGCACATCCACTTTCACCGGCGCATAGCCGCCAACTCGTTAAAACTGACCGTCTGCCAGCACGCGCCGCAAACGCGGCTGCCCATCCAGCGGCCTAACACCAATTTCGTCGAGGAAGAATg                                                                                >  5:39942/1‑148 (MQ=255)
                                                                      cTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATc                                                                                                       >  7:252086/1‑120 (MQ=255)
                                                                      cTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATc                                                                                                       <  8:252086/120‑1 (MQ=255)
                                                                            aGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGAATAATGTACCGCCATCg                                                                     <  3:173313/148‑1 (MQ=255)
                                                                                  aGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGAATAATGTACCGCCATCGGCCTGtt                                                              >  3:140205/1‑149 (MQ=255)
                                                                                       tAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGAATAATGTACCGCCATCGGCCTGTTCaaaa                                                         <  2:268246/149‑1 (MQ=255)
                                                                                        aaTCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGAATAATGTACCGCCATCGGCCTGTTCAAAAc                                                        >  4:291221/1‑149 (MQ=255)
                                                                                             gCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGAATAATGTCCCGCCATCGGCCTGTTCAAAACGcccc                                                   >  6:64867/1‑149 (MQ=255)
                                                                                             gCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGAATAATGTACCGCCATCGGCCTGTTCAAAACGcccc                                                   >  2:263664/1‑149 (MQ=255)
                                                                                             gCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGAATAATGTACCGCCATCGGCCTGTTCAAAACGcccc                                                   >  4:252604/1‑149 (MQ=255)
                                                                                                caTCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGAATAATGTACCGCCATCGGCCTGTTCAAAACGCCCCTGa                                                <  1:204119/149‑1 (MQ=255)
                                                                                                caTCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTACCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGAATAATGTACCGCCATCGGCCTGTTCAAAACGCCCCTGa                                                <  5:115732/149‑1 (MQ=255)
                                                                                                caTCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTACCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGAATAATGTACCGCCATCGGCCTGTTCAAAACGCCCCTGa                                                <  5:120968/149‑1 (MQ=255)
                                                                                                     aCTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGAATAATGTACCGCCATCGGCCTGTTCAAAACGCCCCTGACGAAt                                           >  2:252059/1‑149 (MQ=255)
                                                                                                       tttCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGaata                                                                                   >  8:334096/1‑107 (MQ=255)
                                                                                                       tttCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGaata                                                                                   <  7:334096/107‑1 (MQ=255)
                                                                                                            ccGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGAGGCATAACAAAAATTTCGTCGAGGAATAATGTACCTCCATCGGCCTGATCAAAACGCCCCTGACGAATGGTAtac                                    >  1:254838/1‑147 (MQ=255)
                                                                                                             cGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGAATAATGTACCGCCATCGGCCTGTTCAAAACGCCCCTGACGAATGGTATTcg                                   >  4:37739/1‑149 (MQ=255)
                                                                                                              ggCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGAATAATGTACCGCCATCGGCCTGTTCAAAACGCCCCTGACGAATGGTATTcgc                                  >  4:68681/1‑149 (MQ=255)
                                                                                                                cgcATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGAATAATGTACCGCCATCGGCCTGTTCAAAACGCCCCTGACGAATGGTATTCgcgc                                <  1:82639/149‑1 (MQ=255)
                                                                                                                  cATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGAATAATGTACCGCCATCGGCCTGTTCAAAACGCCCCTGACGAATGGTATTCGCGCCa                              <  5:50386/149‑1 (MQ=255)
                                                                                                                        cgccAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGAATAATGTACCGCCATCGGCCTGTTCAAAACGCCCCTGACGAATGGTATTCGCGCCAGTAAAc                        <  3:328190/149‑1 (MQ=255)
                                                                                                                          ccAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCACACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGAATAATGTACCGCCATCGGCCTGTTCAAAACGCCCCTGACGAATGGTATTCGCGCCAGTAAAcgc                      >  4:295612/1‑149 (MQ=255)
                                                                                                                           cAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGAATAATGTACCGCCATCGGCCTGTTCAAAACGCCCCTGACGAATGGTATTCGCGCCAGTAAAcgcg                     >  8:311166/1‑149 (MQ=255)
                                                                                                                            aaCGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGAATAATGTACCGCCATCGGCCTGTTCAAAACGCCCCTGACGAATGGTATTCGCGCCAGTAAACgcgc                    >  6:190492/1‑149 (MQ=255)
                                                                                                                                   tAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGAATAATGTACCGCCATCGGCCTGTTCAAAACGCCCCTGACGAATGGTATTCGCGCCAGTAAACGCGCCTTtctc             >  7:85942/1‑149 (MQ=255)
                                                                                                                                     aaaCTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGAATAATGTACCGCCATCGGCCTGTTCAAAACGCCCCTGACGAATGGTATTCGCGCCAGTAAACGCGCCTTTCTCGt           <  2:234604/149‑1 (MQ=255)
                                                                                                                                      aaCTGACCGGATGCCAGCACGCGCATCAAACGCGTCTGCACATCCAGCTGCATATCACCAATTTCGTCGAGGAATAATGTACCGCCATCGGCGTGTTCAAATCGCCCCTGACTAATGGTATTCGCGCCAGTAAACGCGCCTTTCTCGTg          <  6:105365/149‑1 (MQ=255)
                                                                                                                                       aCTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGAATAATGTACCGCCATCGGCCTGTTCAAAACGCCCCTGACGAATGGTATTCGCGCCAGTAAACGCGCCTTTCTCGTgg         <  8:55357/149‑1 (MQ=255)
                                                                                                                                           aCCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATAACCAATTTCGTCGAGGAATAATGTACCGCCATCGGCCTGTTCAAAACGCCCCTGACGAATGGTATTCGCGCCAGTAAACGCGCCTTTCTCGTGGCCa      >  8:317076/1‑148 (MQ=255)
                                                                                                                                             cGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGAATAATGTACCGCCATCGGCCTGTTCAAAACGCCCCTGACGAATGGTATTCGCGCCAGTAAACGCGCCTTTCTCGTGGCCaaa    >  3:51554/1‑148 (MQ=255)
                                                                                                                                              gTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGAATAATGTACCGCCATCGGCCTGTTCAAAACGCCCCTGACGAATGGTATTCGCGCCAGTAAACGCGCCTTTCTCGTGGCCAAACa  <  7:211567/149‑1 (MQ=255)
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CGCGGATAACGTTCAGGCGGTGGAACAGATCCTCACGGAACTTACCTTCCTGCACTCGCTGTTCGAGATTCTGGTGAGTGGCAGCGATAATCCGCACATCCACTTTCACCGGCGCATAGCCGCCAACGCGGTAAAACTGACCGTCTGCCAGCACGCGCAGCAAACGCGTCTGCACATCCAGCGGCATATCACCAATTTCGTCGAGGAATAATGTACCGCCATCGGCCTGTTCAAAACGCCCCTGACGAATGGTATTCGCGCCAGTAAACGCGCCTTTCTCGTGGCCAAACA  >  NC_000913/4054363‑4054653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: