Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,892,368 A→G 6.4% M252V (ATG→GTG)  mdtL → multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,892,3680AG 6.4% 285.7 / 7.6 94M252V (ATG→GTG) mdtLmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base A (47/41);  new base G (3/3);  total (50/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

TTTTTTCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGC  >  NC_000913/3892220‑3892512
                                                                                                                                                    |                                                                                                                                                
ttttttCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCa                                                                                                                                                  >  5:125357/1‑149 (MQ=255)
      ccTCAGCCGTGTTGTTATCACAACCCTCAGCGTTTCGGTGATCCACACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCg                                                                                                                                            >  6:293759/1‑149 (MQ=255)
          aGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGtt                                                                                                                                          <  7:313174/147‑1 (MQ=255)
          aGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTAAACACGTCACCTGTATTCCTGATGGAAATCATGGGGTTTGAGCGCTGAGAATAAGCCACCATTATGGCGCTGACAGCTGGCGTCAGCATGACCGttt                                                                                                                                         >  1:201367/1‑148 (MQ=255)
           gCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAAAACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCa                                                                                                                                       >  6:153522/1‑149 (MQ=255)
             cGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCAtt                                                                                                                                     <  4:266372/149‑1 (MQ=255)
             cGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCAtt                                                                                                                                     <  5:73419/149‑1 (MQ=255)
                 ttgttATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTcc                                                                                                                                 <  2:130452/149‑1 (MQ=255)
                   gttATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCa                                                                                                                                <  7:317722/148‑1 (MQ=255)
                      aTCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGc                                                                                                                             >  1:146139/1‑148 (MQ=255)
                       tCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCGTGACCGtt                                                                                                                                          <  2:120696/134‑1 (MQ=255)
                       tCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCGTGACCGtt                                                                                                                                          >  1:120696/1‑134 (MQ=255)
                       tCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCa                                                                                                                           >  4:155670/1‑149 (MQ=255)
                       tCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCa                                                                                                                           >  1:572/1‑149 (MQ=255)
                         accaccCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCAtt                                                                                                                         <  2:137380/149‑1 (MQ=255)
                         accaccCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCAtt                                                                                                                         >  5:92726/1‑149 (MQ=255)
                           caccCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTg                                                                                                                       >  4:282383/1‑149 (MQ=255)
                              ccTCAGCGTTTCGGTGATCGTCACTTTGGTCAACACGTCTCCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCATCATGACCGTTTCATTCTCCACGCCATTTgcgc                                                                                                                    <  2:201367/149‑1 (MQ=255)
                              ccTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTgcgc                                                                                                                    >  6:316105/1‑149 (MQ=255)
                              ccTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTgcgc                                                                                                                    <  7:337401/149‑1 (MQ=255)
                               cTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCGTGACCGTTTCATTCTCCACGCCATTTGCGCt                                                                                                                   >  8:223163/1‑149 (MQ=255)
                               cTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCGTGACCGTTTCATTCTCCACGCCATTTGCGCt                                                                                                                   >  4:291532/1‑149 (MQ=255)
                               cTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGttt                                                                                                                                         >  5:182226/1‑127 (MQ=255)
                               cTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGttt                                                                                                                                         <  6:182226/127‑1 (MQ=255)
                               cTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCt                                                                                                                   >  5:46778/1‑149 (MQ=255)
                                tCAGCTTTTCGGAGATCCTCACTTTCGTCAACACGTCACCGGTAATGCTGATGGAAATCATTGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTg                                                                                                                  >  6:322632/1‑149 (MQ=255)
                                  atgggttCGGTGATCCTCACGTTCGGCAACACGTCACCGGTTTTGCTGATGGAAAGCTTGGGGTTTCAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTCCGCTgg                                                                                                                 <  8:325298/143‑1 (MQ=255)
                                  aGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTg                                                                                                                  <  6:125357/147‑1 (MQ=255)
                                  aGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTg                                                                                                                  <  3:282383/147‑1 (MQ=255)
                                      tttCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAAtt                                                                                                            >  4:5394/1‑149 (MQ=255)
                                         cGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAAtttt                                                                                                          <  6:92726/148‑1 (MQ=255)
                                          ggTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAAttttt                                                                                                         >  7:262109/1‑148 (MQ=255)
                                          ggTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCAGATGGAAATCATGGGGTTGAGCGCGGTGAATACGCCACCATTATTGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAAttttt                                                                                                         >  1:43359/1‑147 (MQ=255)
                                           gTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCGTGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTaa                                                                                                       <  7:223163/149‑1 (MQ=255)
                                           gTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCGTGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTaa                                                                                                       <  3:291532/149‑1 (MQ=255)
                                           gTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGCTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTaa                                                                                                       <  8:215869/149‑1 (MQ=255)
                                            tGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAg                                                                                                      >  8:115050/1‑149 (MQ=255)
                                              aTCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGcc                                                                                                    <  8:262109/149‑1 (MQ=255)
                                               tCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCa                                                                                                   <  7:31121/149‑1 (MQ=255)
                                               tCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCa                                                                                                   >  7:157688/1‑149 (MQ=255)
                                                  tCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCacg                                                                                                 >  4:228589/1‑148 (MQ=255)
                                                          gTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTtga                                                                                         <  2:572/148‑1 (MQ=255)
                                                             aaCACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTgatgat                                                                                     <  7:285442/149‑1 (MQ=255)
                                                             aaCACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTgatgat                                                                                     <  8:157688/149‑1 (MQ=255)
                                                              acacGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATc                                                                                    <  2:23824/149‑1 (MQ=255)
                                                                acGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCAc                                                                                  <  5:316105/149‑1 (MQ=255)
                                                                   tCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTc                                                                               >  7:34715/1‑149 (MQ=255)
                                                                      ccGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCTCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCg                                                                              >  6:239999/1‑147 (MQ=255)
                                                                       cGGTATTGCTGATGGAAATGATGGGGTTTGTGCGCGGTGAATACGCCACCTTTATGGCGCTGACCGCTGGCGTCAGCATGTGCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTtgat                                                                                        <  6:172918/136‑1 (MQ=255)
                                                                       cGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACTCCCCCATTATGGCGCAGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCAACCAATTTGCGCTGGGAATTTTTAAGCCCCGTACGTtgat                                                                                        >  5:172918/1‑136 (MQ=255)
                                                                       cGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCAt                                                                                                                                      <  6:210770/90‑1 (MQ=255)
                                                                       cGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCAt                                                                                                                                      >  5:210770/1‑90 (MQ=255)
                                                                       cGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAg                                                                           <  7:115050/149‑1 (MQ=255)
                                                                       cGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAg                                                                           >  8:41563/1‑149 (MQ=255)
                                                                       cGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAg                                                                           >  4:114310/1‑149 (MQ=255)
                                                                              acTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTa                                                                     <  1:218627/147‑1 (MQ=255)
                                                                                tGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACAATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTAtt                                                                   >  5:6320/1‑148 (MQ=255)
                                                                                 gATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGttt                                                                                                                                         >  6:22656/1‑77 (MQ=255)
                                                                                 gATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCCCGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTcc                                                                 >  7:258977/1‑149 (MQ=255)
                                                                                  aTGGAAATCATGGGGTTTGATCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCGCGCCCTTTGAGGTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCt                                                                >  2:158012/1‑149 (MQ=255)
                                                                                  aTGGAAAGCATGGGGGTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTcc                                                                 <  6:248306/148‑1 (MQ=255)
                                                                                   tGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTAACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTg                                                               >  6:78215/1‑149 (MQ=255)
                                                                                    ggAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTgg                                                              >  3:313395/1‑149 (MQ=255)
                                                                                     gAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTggc                                                             <  3:114310/149‑1 (MQ=255)
                                                                                          cATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGcggcgg                                                        >  6:292783/1‑149 (MQ=255)
                                                                                           aTGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCtgg                                                       >  4:175942/1‑149 (MQ=255)
                                                                                               ggTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGAt                                                    >  5:230190/1‑148 (MQ=255)
                                                                                                   tGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCAct                                                <  5:153522/148‑1 (MQ=255)
                                                                                                    gAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCActct                                              >  7:346583/1‑149 (MQ=255)
                                                                                                    gAGCGCGGTGAATACGCCACCATTATGGCGCAGACCGCTGGCGTCAGCATGACCGTTTCATTCTCACCGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTCTTATTCCTGGCGGCGGGGATCAcact                                              >  4:88701/1‑149 (MQ=255)
                                                                                                     aGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCtt                                             <  3:228589/149‑1 (MQ=255)
                                                                                                      gcgcGGTGCATACGCCACCATTATGGCGCTGACCGCTGGCGACAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGATGATGATCACCTCGCAGTTTATATTCCTGGCGGCGGGGATCACTCTTg                                            >  7:258728/1‑149 (MQ=255)
                                                                                                                tACGCCACCATTATGGCGCTGACCGCTGGCGGCAGCAGGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGGACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCAcc                                  <  5:293759/149‑1 (MQ=255)
                                                                                                                 aCGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTGTTGCCGTTTCACCt                                 <  5:322632/149‑1 (MQ=255)
                                                                                                                  cGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCtt                                <  3:155670/149‑1 (MQ=255)
                                                                                                                   gCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTc                               >  6:154718/1‑149 (MQ=255)
                                                                                                                    ccaccaTTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCAACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCTGGGATCCCTCTTGACGTTTCACATTcc                              >  8:323651/1‑149 (MQ=255)
                                                                                                                      accaTTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCa                            >  3:37509/1‑149 (MQ=255)
                                                                                                                        caTTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATg                          >  8:335276/1‑149 (MQ=255)
                                                                                                                        caTTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATg                          <  5:154718/149‑1 (MQ=255)
                                                                                                                        caTTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATg                          <  5:313223/149‑1 (MQ=255)
                                                                                                                             tGGCTCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGt                      <  5:239999/148‑1 (MQ=255)
                                                                                                                             tGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGt                      <  5:292783/148‑1 (MQ=255)
                                                                                                                               gcgcTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGttt                    <  7:41563/148‑1 (MQ=255)
                                                                                                                                 gcTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTtctc                 >  3:189474/1‑149 (MQ=255)
                                                                                                                                 gcTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTtctc                 >  3:152246/1‑149 (MQ=255)
                                                                                                                                  cTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTACCCATGCGGTTTctct                >  4:252367/1‑149 (MQ=255)
                                                                                                                                      ccGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGttt            >  7:305933/1‑149 (MQ=255)
                                                                                                                                      ccGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTAGCAGGTGTTATTCCTGGAGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGttt            <  6:6320/149‑1 (MQ=255)
                                                                                                                                       cGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTg           >  5:279435/1‑149 (MQ=255)
                                                                                                                                         cTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTTGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGGTTgg          >  1:322119/1‑148 (MQ=255)
                                                                                                                                         cTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGt         <  8:346583/149‑1 (MQ=255)
                                                                                                                                             cGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCa     <  3:5394/149‑1 (MQ=255)
                                                                                                                                               tCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTACGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACg   >  5:7267/1‑149 (MQ=255)
                                                                                                                                                 aGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGc  <  8:305933/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
TTTTTTCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGC  >  NC_000913/3892220‑3892512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: