Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,792,765 C→T 5.6% intergenic (‑214/+61) kbl ← / ← yibB glycine C‑acetyltransferase/YibB family protein, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,792,7650CT 5.6% 340.1 / 5.9 106intergenic (‑214/+61)kbl/yibBglycine C‑acetyltransferase/YibB family protein, function unknown
Reads supporting (aligned to +/- strand):  ref base C (49/51);  new base T (3/3);  total (52/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.26e-01

Only 100 of 111 total aligned reads displayed.

GCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTT  >  NC_000913/3792619‑3792913
                                                                                                                                                   |                                                                                                                                                    
gcgTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCa                                                                                                                                                     >  3:161843/1‑148 (MQ=255)
gcgTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCa                                                                                                                                                     >  2:195431/1‑148 (MQ=255)
 cgTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCaaa                                                                                                                                                   <  1:124238/149‑1 (MQ=255)
 cgTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCaaa                                                                                                                                                   <  8:27058/149‑1 (MQ=255)
     aaCACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGca                                                                                                                                                <  3:118360/148‑1 (MQ=255)
      acacTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGcat                                                                                                                                               >  8:316789/1‑148 (MQ=255)
          ttCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCTTTGGCATCAttt                                                                                                                                          >  1:134356/1‑149 (MQ=255)
            cTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCAtttt                                                                                                                                         <  3:29763/148‑1 (MQ=255)
             tGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTc                                                                                                                                       >  6:53728/1‑149 (MQ=255)
               cAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCTTTGGCATCAttttttc                                                                                                                                      <  4:25790/148‑3 (MQ=255)
               cAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCTTTGGCATCATTTTTTCt                                                                                                                                     <  2:134356/149‑1 (MQ=255)
               cAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTca                                                                                                                                      >  4:246691/1‑147 (MQ=255)
               cAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCt                                                                                                                                     <  4:205900/149‑1 (MQ=255)
               cAAAATTCAGCATAAATAACGTCTAACTAGATCGCTCTAAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATCATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTc                                                                                                                                       <  2:147392/147‑1 (MQ=255)
                aaaaTTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTa                                                                                                                                    >  3:249392/1‑149 (MQ=255)
                  aaTTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGCTTTGGCTGCGCCTAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTAtc                                                                                                                                  <  7:238679/149‑1 (MQ=255)
                   aTTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTAtct                                                                                                                                 >  7:189269/1‑149 (MQ=255)
                         gATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTg                                                                                                                           >  7:34292/1‑149 (MQ=255)
                           taaataaCGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGt                                                                                                                         <  1:195431/149‑1 (MQ=255)
                             aataaCGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTa                                                                                                                        <  7:287005/148‑1 (MQ=255)
                               taaCGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATc                                                                                                                      >  7:290784/1‑148 (MQ=255)
                                  agtgtAACTTGATGTCTCTCAACAACTACAGATAGTAGAAAAACTGTACAGATTTGGCTGCGCATCTAGTTGATTGATTTTACTGCATTGATATAAAAAGAAATATTGATCCCAAAGCATCATTTTTCCTATCTCAGTTTGAATCaaat                                                                                                                  <  2:147333/148‑2 (MQ=255)
                                   gtgtAACTTGATGTCTCTCAACACCTACATTTATTATAAAAACTGTACAGTTTTTGCTGGGCATAATGATTATTGATTTTTCTGCATTGATATAAAAATAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAAc                                                                                                                 <  5:187311/149‑1 (MQ=255)
                                   gtgtAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAAc                                                                                                                 <  5:333862/149‑1 (MQ=255)
                                   gtgtAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAAc                                                                                                                 <  8:58701/149‑1 (MQ=255)
                                     gtAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGc                                                                                                               >  2:328748/1‑149 (MQ=255)
                                        acaagATGTCTCTCAACACCTACAGATACTAGAAAAACTGAACACTTTTGGCTCGGCATAAAGATGATAGATTTTACTGCATTGATATAAAAAGAAAAAAAGAAGCCAAAGCATGATTTATCCTATCTCAGTTGGTATCAAAACGCAAc                                                                                                            >  4:209257/5‑149 (MQ=255)
                                        aCTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTa                                                                                                             <  4:301319/148‑1 (MQ=255)
                                        aCTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTa                                                                                                             <  5:236459/148‑1 (MQ=255)
                                        aCTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTa                                                                                                             <  2:64745/148‑1 (MQ=255)
                                          ttGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTAcc                                                                                                           >  8:208683/1‑148 (MQ=255)
                                             aTGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCAc                                                                                                        <  7:187324/148‑1 (MQ=255)
                                             aTGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATCATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCATATCTCAGTTGGTATCAAAACGCTACCCAc                                                                                                        >  6:305867/1‑148 (MQ=255)
                                                 ctctcAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAAt                                                                                                    <  8:189269/148‑1 (MQ=255)
                                                  tctcAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGATGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAATAAAAAATGATGCCAACGCATCATTTTTCCTATCTCAGTTGTGATCAAAACGCTACCCACTAATg                                                                                                   >  5:208825/1‑148 (MQ=255)
                                                    tcAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAACAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATg                                                                                                   <  1:223378/146‑1 (MQ=255)
                                                    tcAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCTTTGGCATCAttttttc                                                                                                                                      >  1:160192/1‑109 (MQ=39)
                                                    tcAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCTTTGGCATCAttttttc                                                                                                                                      <  2:160192/111‑3 (MQ=39)
                                                    tcAACAACTACATATATTATAAAAACTTTACAGTTTTGGATGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATg                                                                                                   <  1:160696/146‑1 (MQ=255)
                                                      aaCACCTACAGATAGTATAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTa                                                                                                             <  5:173226/134‑1 (MQ=255)
                                                      aaCACCTACAGATAGTAGAATAACTGTACAGTTTTGGCTGGGAATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAg                                                                                                <  6:64594/147‑1 (MQ=255)
                                                      aaCACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTa                                                                                                             >  6:173226/1‑134 (MQ=255)
                                                      aaCACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAg                                                                                                <  5:309194/147‑1 (MQ=255)
                                                           cTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAAc                                                                                                                 >  7:123984/1‑125 (MQ=255)
                                                               aGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTAtt                                                                                     >  3:136201/1‑149 (MQ=255)
                                                                 aTAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAAc                                                                                          <  2:145551/142‑1 (MQ=255)
                                                                 aTAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAAc                                                                                          >  1:145551/1‑142 (MQ=255)
                                                                 aTAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTAtttt                                                                                   <  7:208683/149‑1 (MQ=255)
                                                                  tagtagAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTc                                                                                  <  8:34292/149‑1 (MQ=255)
                                                                    gtagAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCtt                                                                                <  7:316789/149‑1 (MQ=255)
                                                                    gtagAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCtt                                                                                <  8:290784/149‑1 (MQ=255)
                                                                        aaaaaCTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGa                                                                              >  4:236807/1‑147 (MQ=255)
                                                                         aaaaCTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAg                                                                           >  2:26147/1‑149 (MQ=255)
                                                                             cTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGa                                                                         >  2:79451/1‑148 (MQ=255)
                                                                             cTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGaa                                                                        >  6:69214/1‑148 (MQ=255)
                                                                                 aCAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGTAAACCTATTTTCTTGAAAGGAAAATtc                                                                   >  7:288461/1‑149 (MQ=255)
                                                                                 aCAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATtc                                                                   >  7:45102/1‑149 (MQ=255)
                                                                                   aGTTTTGGCTTGGCATAATGAAGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATAATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATtctc                                                                 <  1:75177/149‑1 (MQ=255)
                                                                                   aGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATtctc                                                                 <  1:79799/149‑1 (MQ=255)
                                                                                    gTTTTGGCTGGTCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAACTGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCa                                                                <  7:340448/149‑1 (MQ=255)
                                                                                          gCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCt                                                          >  2:159536/1‑149 (MQ=255)
                                                                                          gCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCt                                                          <  5:78049/149‑1 (MQ=255)
                                                                                             gggCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCa                                                       >  2:75230/1‑149 (MQ=255)
                                                                                                  tacggatgatTGATGTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAATCATCATTTTTCCTATCTCAGTTGGTAGCAAAACGCTACACACTAATGGAGGGAAACCTATTTTCTTGCAAGGAAAATTCTCAGCGCCTGCAttttt                                                  <  6:208825/145‑1 (MQ=255)
                                                                                                  tAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCAttttt                                                  <  4:179613/149‑1 (MQ=255)
                                                                                                    atgatgATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGc                                                <  5:69214/149‑1 (MQ=255)
                                                                                                       atgatTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGccc                                              <  5:12633/148‑1 (MQ=255)
                                                                                                           ttgattTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTaaa                                         <  5:53728/149‑1 (MQ=255)
                                                                                                           ttgattTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTaaa                                         >  3:187708/1‑149 (MQ=255)
                                                                                                              attTTACTGCATTGATATAAAAAGAAAAACTGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGAATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAgtg                                      <  3:216844/149‑1 (MQ=255)
                                                                                                              attTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAgtg                                      >  7:331320/1‑149 (MQ=255)
                                                                                                               ttttACTGCATTGATATAAAAAGAAAAAATGATGCCTAAGCATCATTTTTCCTATCTCATTTTGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTATTGAAAGGAAAATTCTCAGCTCCTGCATTTTTGCCCCTAAAgtg                                      <  6:105167/148‑1 (MQ=255)
                                                                                                               ttttACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAgtgt                                     >  3:207807/1‑149 (MQ=255)
                                                                                                               ttttACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAgtgt                                     >  5:339661/1‑149 (MQ=255)
                                                                                                               ttttACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAgtgt                                     <  4:136201/149‑1 (MQ=255)
                                                                                                                  tACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAATGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTa                                   <  4:249392/148‑1 (MQ=255)
                                                                                                                   aCTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAACCCTATTTTCTTGAAAGGAAAATTCTCCGCGCCTGCCTTTTTGCCCCTAAAGTGTTACt                                 >  6:162702/1‑149 (MQ=255)
                                                                                                                        aTTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTg                             >  2:12424/1‑148 (MQ=255)
                                                                                                                                aaaaaGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTCCCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCg                     >  8:265018/1‑148 (MQ=255)
                                                                                                                                aaaaaGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTa                                   >  3:148032/1‑134 (MQ=255)
                                                                                                                                aaaaaGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTa                                   <  4:148032/134‑1 (MQ=255)
                                                                                                                                 aaaaGAAAAAATGATGCCAAAGCCTCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGa                    >  5:53341/1‑148 (MQ=255)
                                                                                                                                 aaaaGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGa                    >  3:118109/1‑148 (MQ=255)
                                                                                                                                   ataggaaaaaTGATGCTTTGGCATCATTTTTTCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGaaa                  >  5:75893/6‑149 (MQ=255)
                                                                                                                                   aaGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTTTCAAACCGCTACCCACGAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTACAGTGTTAAGCCTGAAGCAGCGcaa                  >  4:222958/1‑148 (MQ=255)
                                                                                                                                   aaGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAAc                 <  1:328748/149‑1 (MQ=255)
                                                                                                                                   aaGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAAc                 <  6:252313/149‑1 (MQ=255)
                                                                                                                                       aaaaaaTGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGGATCAAAACGCTACCCACTAATGGAGGGAACCCTAGTTTCTTGACAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCAAAAGTGTTACTCCTCAAGCAGAGAAACaa               >  5:215274/1‑148 (MQ=255)
                                                                                                                                        aaaaaTGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAAt             <  5:239740/149‑1 (MQ=255)
                                                                                                                                         aaaaTGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATc            <  3:236807/149‑1 (MQ=255)
                                                                                                                                          aaaTGATGCCAACGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACACACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAATTGTTACTCCTGAAGCAGCGAAACaaagc            >  5:274908/1‑146 (MQ=255)
                                                                                                                                          aaaTGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATc            >  3:32579/1‑148 (MQ=255)
                                                                                                                                           aaTGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCg           >  8:137245/1‑148 (MQ=255)
                                                                                                                                            atgatgCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGaa         >  5:291309/1‑149 (MQ=255)
                                                                                                                                            atgatgCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGaa         <  6:339661/149‑1 (MQ=255)
                                                                                                                                            atgatgCCAAAGCATCATTTTTCCGATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCGTGAAAGGAAATTTCTCAGCGCCTTCATTTTTGCCCCTAAAGTGTTACTCATGAAGCAGCGAAACAAATctac         >  5:260239/1‑146 (MQ=255)
                                                                                                                                                   cAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAgcgc                                                            >  7:97053/1‑91 (MQ=255)
                                                                                                                                                   cAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTc                                >  5:234835/1‑119 (MQ=255)
                                                                                                                                                   cAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTc                                <  6:234835/119‑1 (MQ=255)
                                                                                                                                                   cAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCAtttt  >  5:170298/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTT  >  NC_000913/3792619‑3792913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: