Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	564,107	IS2 (+) +5 bp	coding (370‑374/633 nt)	sfmZ ←	response regulator family protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	381260 =	NA (NA)	51 (1.300)	41/268	0.1	100%	noncoding (1/1331 nt)	IS2	repeat region
	?	NC_000913	= 564111	0 (0.000)					coding (370/633 nt)	sfmZ	response regulator family protein
*	?	NC_000913	= 382590	NA (NA)	38 (0.960)	36/270	0.2	100%	noncoding (1331/1331 nt)	IS2	repeat region
	?	NC_000913	564107 =	0 (0.000)					coding (374/633 nt)	sfmZ	response regulator family protein

CGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/381408‑381260 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aTACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATGTAGTACGAATAGTTCAACGGTCACTGTGCTATCTAATCGTGAAGTGACCATATTACGTTATCTGGTTAGCGGATTATCTAATAAAGAAATTG  <  NC_000913/564111‑563970                                                                                                                                                                                                                                                                                                     CGATCTTTTCCTGTGTGGTACGCCGTCTGCTTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTTGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTA                                                                                                                                                <  4:96204/149‑1  GATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTA                                                                                                                                                >  4:148784/1‑148              TGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTC                                                                                                                                   >  8:137073/1‑149                      GCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGA                                                                                                                            >  5:86394/1‑148                        CGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAAC                                                                                                                         >  2:199174/1‑149                            TGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCT                                                                                                                      >  1:13828/1‑148                            TGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCA                                                                                                                                <  1:97086/138‑1                            TGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCA                                                                                                                                >  2:97086/1‑138                                         CCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAA                                                                                                        <  5:7168/149‑1                                         CCCTAAGACATCAACCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAA                                                                                                        <  5:78660/149‑1                                                     AATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATG                                                                                              >  8:14257/1‑147                                                      ATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATGTAG                                                                                           <  1:199174/149‑1                                                      ATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATGTAG                                                                                           <  1:14795/149‑1                                                           CTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTA                                                                                                                                                >  3:57351/1‑91                                                           CTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTA                                                                                                                                                <  4:57351/91‑1                                                                            GTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATGTAGTACGAATAGTTCAACGGTCACT                                                                     >  6:170683/1‑149                                                                            GTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATGTAGTACGAATAGTTCAACGGTCACT                                                                     >  3:93074/1‑149                                                                            GTCTAAAAACTACTATTAAGACTATCACTTATTTAAGTCATAATGGTTGTCTGGACAATCAGCGGGCCACTCAATACACGTTTTTTCACAGCGAAACGCTTAACTATATAAAAAGCAATAAATCTAGTACGAAAAGTTCAACGGTCACT                                                                     >  3:59299/1‑149                                                                               TAAAAACTAGTATTTAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATGTAGTACGAATAGTTCAACGGTCACTGTG                                                                  >  8:8353/1‑149                                                                               TAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATGTAGTACGAATAGTTCAACGGTCACTGTG                                                                  >  7:69159/1‑149                                                                               TAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATGTAGTACGAATAGTTCAACGGTCACTGTG                                                                  >  3:24792/1‑149                                                                                      TAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATGTAGTACGAATAGTTCAACGGTCACTGTGCTATCT                                                            >  6:176283/1‑148                                                                                        gttTTAAGTCTTTCAGTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGGAATAAATGTAGTACGAATAGTTCAACGGTGACTGTGCTATCTAA                                                          <  4:59299/145‑1                                                                                         TATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTA                                                                                                                    <  6:101714/89‑1                                                                                         TATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTA                                                                                                                    >  5:101714/1‑89                                                                                           TTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATGTAGTACGAATAGTTCAACGGTCACTGTGCTATCTAATCGT                                                      >  7:45881/1‑149                                                                                           TTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATGTAGTACGAATAGTTCAACGGTCACTGTGCTATCTAATCGT                                                      <  5:176283/149‑1                                                                                           TTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATGTAGTA                                                                                         >  1:94088/1‑114                                                                                           TTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATGTAGTA                                                                                         <  2:94088/114‑1                                                                                            TAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATGTAGTACGAATAGTTCAACGGTCACTGTGCTATCTAATCGT                                                      >  5:99338/1‑148                                                                                             AAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATGTAGTACGAATAGTTCAACGGTCACTGTGCTATCTAATCGTG                                                     >  7:136066/1‑148                                                                                              AGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATGTAGTACGAATAGTTCAACGGTCACTGTGCTATCTAATCGTGAA                                                   <  8:45881/149‑1                                                                                                  TATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCAAGCGAAACGCTTAACTATATAAAAAGCAATAAAGGTAGTACGAATAGTTCAACGGTCACTGTGCTATCTAATCGTGAAGAGA                                               >  2:89028/1‑149                                                                                                      ACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATGTAGTACGAATAGTTCAACGGTCACTGTGCTATCTAATCGTGAAGTGACCA                                            <  4:93074/148‑1                                                                                                        TTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATGTAGTACGAATAGTTCAACGGTCACTGTGCTATCTAATCGTGAAGTGACCATAT                                         >  4:73567/1‑149                                                                                                          ATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATGTAGTACGAATAGTTCAACGGTCACTGTGCTATCTAATCGTGAAGTGACCATATT                                        <  7:14257/148‑1                                                                                                           TTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATGTAGTACGAATAGTTCAACGGTCACTGTGCTATC                                                             >  7:21943/1‑126                                                                                                           TTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATGTAGTACGAATAGTTCAACGGTCACTGTGCTATC                                                             <  8:21943/126‑1                                                                                                               AGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATGTAGTACGAATAGTTCAACGGTCACTGTGCTATCTAATCGTGAAGTGACCATATTACGTTA                                  >  4:50001/1‑149                                                                                                               AGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATGTAGTACGAATAGTTCAACGGTCACTGTGCTATCTAATCGTGAAGTGACCATATTACGTTA                                  >  5:143687/1‑149                                                                                                                 TGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATGAAGTACGAATAGTTCAACGGTCACTGTGCTATCTAATCGTGAAGTGACCATATTACGTTATC                                <  6:86394/149‑1                                                                                                                   ATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAAC‑‑TATAAAAAGCAATAAATGTAGTAAGAATAGTTCAACGGTCACTGTGCTATCTAATCGTGAAGTGACCCAATTAGGGTATATGGT                            >  8:103017/1‑149                                                                                                                    TATTGGTTGTCTGGAGATTCAGGGTGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATGTAGTACGAATAGTTCAACGGTCACTGTGCTATCTAATCGTGAAGTGACCATATTACGTTATCTGG                             <  4:24792/149‑1                                                                                                                    TATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATGTAGTACGAATAGTTCAACGGTCACTGTGCTATCTAATCGTGAAGTGACCATATTACGTTATCTGG                             <  7:8353/149‑1                                                                                                                      TTGGTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATGTAGTACGAATAGTTCAACGGTCACTGTGCTATCTAATCGTGAAGTGACCATATTACGTTATCTGGTT                           <  2:13828/149‑1                                                                                                                         GTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATGTAGTACGAATAGTTCAACGGTCACTGTGCTATCTAATCGTGAAGTGACCATATTACGTTATCTGGTTAGC                        >  3:9144/1‑149                                                                                                                         GTTGTCTGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATGTAGTACGAATAGTTCAACGGTCACTGTGCTATCTAATCGTGAAGTGACCATATTACGTTATCTGGTTAGC                        <  8:69159/149‑1                                                                                                                               TGGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATGTAGTACGAATAGTTCAACGGTCACTGTGCTATCTAATCGTGAAGTGACCATATTACGTTATCTGGTTAGCGGATTA                  >  1:198423/1‑149                                                                                                                                GGAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCCGCGAAACGCTTAACTATATAAAAAGCAATAAATGTAGTCCGAATAGGTCAACGGTCACTGTGCTAGCTAATAGTGAAGTGACCATATTAAGTTATCTGGTT                           >  8:49662/1‑139                                                                                                                                 GAGATTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATGTAGTACGAATAGTTCAACGGTCACTGTGCTATCTAATCGTGAAGTGACCATATTACGTTATCTGGTTAGCGGATT                   <  7:137073/146‑1                                                                                                                                     TTCAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATGTAGTACGAATAGTTCAACGGTCACTGTGCTATCTAATCGTGAAGTGACCATATTACGTTATCTGGTTAGCGGATTATCTAA             <  5:170683/148‑1                                                                                                                                       CAGGGGGCCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATGTAGTACGAATAGTTCAACGGTCACTGTGCTATCTAATCGTGAAGTGACCATATTACGTTATCTGGTTAGCGGATTATCTAATA           <  3:73567/148‑1                                                                                                                                            ggaCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAAC‑‑TATAAAAAGCAATAAATGTAGTACGAATAGTTCAACGGTCACTGTGCTATCTAATCGTGAAGTGACCATATTACGTTATCTGGTTAGCGGATTATCTAATAAAGAAATT   <  7:103017/146‑1                                                                                                                                              CCAGTCTATACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATGTAGTACGAATAGTTCAACGGTCACTGTGCTATCTAATCGTGAAGTGACCATATTACGTTATCTGGTTAGCGGATTATCTAATAAAGAAATT   >  3:66584/1‑149                                                                                                                                               CAGTCTATACACGTTTTTTCCCAGCGAAAGGCTTAACTATATAAAAAGCAATAAATGTAGTACGAATAGTTCAACGGTCAGTGTGCTATCTAATCGTGAAGTGCACATATTACGTTATCTGGTTAGCGGATTATCGAATAAAGAAATTG  >  4:62029/1‑149                                                                                                                                                                                                                                                                                                     CGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/381408‑381260 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aTACACGTTTTTTCCCAGCGAAACGCTTAACTATATAAAAAGCAATAAATGTAGTACGAATAGTTCAACGGTCACTGTGCTATCTAATCGTGAAGTGACCATATTACGTTATCTGGTTAGCGGATTATCTAATAAAGAAATTG  <  NC_000913/564111‑563970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.