Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913755,3350T.64.0% 72.6 / 185.5 100coding (159/390 nt)sdhCsuccinate dehydrogenase, membrane subunit, binds cytochrome b556
Reads supporting (aligned to +/- strand):  ref base T (19/17);  new base . (33/31);  total (52/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 105 total aligned reads displayed.

TGAAAAAACAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGA  >  NC_000913/755190‑755475
                                                                                                                                                 |                                                                                                                                            
tgAAAAAACAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTccc                                                                                                                                           <  4:441731/149‑1 (MQ=255)
  aaaaaaCAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTcccc                                                                                                                                          <  2:343923/148‑1 (MQ=255)
  aaaaaaCAAAGACCTGTTAATCTGGACCTACAGACAATCAGGTTACCCATCACGGCGATAGCGTCCATTATCAATAGCGTTTAAGTTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTAATGGGTACCAGCCTCTCTTccac                                                                                                                                         >  6:339068/1‑147 (MQ=255)
     aaaCAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGaa                                                                                                                                     <  6:97804/149‑1 (MQ=255)
      aaCAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTg                                                                                                                                       >  8:90646/1‑147 (MQ=255)
      aaCAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGaa                                                                                                                                     <  5:339068/149‑1 (MQ=255)
           aGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTc                                                                                                                               <  4:393955/149‑1 (MQ=255)
             aCCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGa                                                                                                                             <  8:182950/149‑1 (MQ=255)
             aCCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGa                                                                                                                             <  4:29109/149‑1 (MQ=255)
                     aTCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCtt                                                                                                                     >  7:90668/1‑149 (MQ=255)
                      tCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTc                                                                                                                    >  4:272662/1‑149 (MQ=255)
                         ggACCTACAGACCATCCGGTTCCCCAACACGGCGATAGCGTCCATTATCCATAGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTGTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCcgc                                                                                                                 >  1:401532/1‑149 (MQ=255)
                             cTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCGGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGAt                                                                                                              >  5:80447/1‑149 (MQ=255)
                             cTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGAt                                                                                                              >  1:260328/1‑149 (MQ=255)
                                cAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGAtt                                                                                                             <  5:246450/147‑1 (MQ=255)
                                 aGACCATACTGGTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATgg                                                                                                         <  6:6935/149‑1 (MQ=255)
                                     cATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGAtt                                                                                                             >  6:1830/1‑141 (MQ=255)
                                     cATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGAtt                                                                                                             <  5:1830/141‑1 (MQ=255)
                                     cATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAg                                                                                                     >  5:194334/1‑149 (MQ=255)
                                          ggTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGcttc                                                                                                 >  5:369998/1‑149 (MQ=255)
                                               cccATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGt                                                                                            >  1:414664/1‑148 (MQ=255)
                                                ccATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGt                                                                                            >  5:94385/1‑148 (MQ=255)
                                                ccATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGt                                                                                            >  2:279534/1‑148 (MQ=255)
                                                ccATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTc                                                                                           >  3:225906/1‑148 (MQ=255)
                                                 cATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTc                                                                                           >  3:390767/1‑148 (MQ=255)
                                                     aCGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAAt                                                                                       <  8:349148/148‑1 (MQ=255)
                                                      cGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGTCTTCTGGGTAGCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTa                                                                                    <  5:139309/149‑1 (MQ=255)
                                                         cGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTCTTGCAGTGGGCATCCTTCAGTGGCTTCTGGGTACCAGCCTCTCTTACCCTGAAGGTTTCGAGCAAGCTTCCGCGATTAAGGGCAGCTTCTTCGTCAAAATTAt                                                                                   >  2:52235/1‑148 (MQ=255)
                                                             aGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGc                                                                                                       >  8:167729/1‑123 (MQ=255)
                                                             aGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGc                                                                                                       <  7:167729/123‑1 (MQ=255)
                                                                gTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAgg                                                                                                                                   <  8:185062/92‑1 (MQ=255)
                                                                gTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAgg                                                                                                                                   >  7:185062/1‑92 (MQ=255)
                                                                    aTTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCctctctcc                                                                                                                                            <  5:205997/80‑3 (MQ=255)
                                                                    aTTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCctctctcc                                                                                                                                            >  6:205997/1‑78 (MQ=255)
                                                                     ttCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGCGGGGCATcc                                                                     >  1:300832/1‑149 (MQ=255)
                                                                        tcCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTa                                                                  >  7:82066/1‑149 (MQ=255)
                                                                         ccATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCttcttc                                                                                              <  1:104925/121‑1 (MQ=255)
                                                                         ccATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCttcttc                                                                                              >  2:104925/1‑121 (MQ=255)
                                                                          cATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAAttt                                                                                     <  2:129554/128‑1 (MQ=255)
                                                                          cATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAAttt                                                                                     >  1:129554/1‑128 (MQ=255)
                                                                          cATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTa                                                                  <  5:218510/147‑1 (MQ=255)
                                                                          cATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTAcc                                                                >  3:233858/1‑149 (MQ=255)
                                                                           aTCGCGTTTGCGGTGGGATCACCTTTGTTGCAGTGTGCATCCTGCTGTGGCTTCTGTGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCTATTTTATCATGTGGGGCATCCTTAcc                                                                <  1:52235/149‑1 (MQ=255)
                                                                           aTCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATc                                                                                  <  3:290947/131‑1 (MQ=255)
                                                                           aTCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATc                                                                                  >  4:290947/1‑131 (MQ=255)
                                                                           aTCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGc                                                                                                    <  3:265320/112‑1 (MQ=255)
                                                                           aTCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGc                                                                                                    >  4:265320/1‑112 (MQ=255)
                                                                           aTCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCttcttc                                                                                              >  8:375100/1‑118 (MQ=255)
                                                                           aTCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCttcttc                                                                                              <  7:375100/118‑1 (MQ=255)
                                                                            tCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAg                                                                                                                        <  8:120527/91‑1 (MQ=255)
                                                                            tCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAg                                                                                                                        >  7:120527/1‑91 (MQ=255)
                                                                               cgTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGctct                                                           <  2:414664/149‑1 (MQ=255)
                                                                                            aTCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCAcgtc                                              >  5:2275/1‑149 (MQ=255)
                                                                                            aTCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCAcgtc                                              <  3:149149/149‑1 (MQ=255)
                                                                                            aTCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCAcgtc                                              <  6:194334/149‑1 (MQ=255)
                                                                                              cACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTCCCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCAcgtcgt                                            >  6:250006/1‑149 (MQ=255)
                                                                                               aCCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATc                                                   <  3:260174/141‑1 (MQ=255)
                                                                                               aCCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATc                                                   >  4:260174/1‑141 (MQ=255)
                                                                                               aCCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCAcgtcgtc                                           >  4:67984/1‑149 (MQ=255)
                                                                                                ccTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCAcgtcgtcg                                          <  8:82066/149‑1 (MQ=255)
                                                                                                 cTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCAcgtcgtcg                                          >  2:429939/1‑148 (MQ=255)
                                                                                                 cTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCAcgtcgtcg                                          >  4:65932/1‑148 (MQ=255)
                                                                                                  tttGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTAt                                                                                   >  4:234833/1‑107 (MQ=255)
                                                                                                  tttGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTAt                                                                                   <  3:234833/107‑1 (MQ=255)
                                                                                                  tttGTTGCAGTGGGCATCCTGCTGTGGCTTCTGCGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATTGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTCGCGTATCACGTCgtagta                                        >  7:368804/1‑149 (MQ=255)
                                                                                                    tgttgCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCAcgtcgtcgt                                         <  2:260328/147‑1 (MQ=255)
                                                                                                    tgttgCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAg                                       <  4:225906/148‑1 (MQ=255)
                                                                                                     gttgCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGaa                                                                                                                                     <  1:250446/54‑1 (MQ=255)
                                                                                                     gttgCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGaa                                                                                                                                     >  2:250446/1‑54 (MQ=255)
                                                                                                     gttgCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAg                                       <  1:279534/148‑1 (MQ=255)
                                                                                                      ttgCAGTGGGCATACTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCGTGCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAgg                                      >  5:205148/1‑148 (MQ=255)
                                                                                                        gCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTAtt                                  >  1:259587/1‑149 (MQ=255)
                                                                                                         cAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTc                                 >  3:2600/1‑149 (MQ=255)
                                                                                                         cAGTGGGCAGCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCAGCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTc                                 <  8:127089/149‑1 (MQ=255)
                                                                                                              ggCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCca                             >  5:375724/1‑149 (MQ=255)
                                                                                                              ggCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGGATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCcac                            <  8:376724/149‑1 (MQ=255)
                                                                                                              ggCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTGTGGCGTATCACGTCGTCGTAGGTATTCGCca                             <  2:401532/148‑1 (MQ=255)
                                                                                                                cATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACat                          >  8:348176/1‑149 (MQ=255)
                                                                                                                  tCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGAGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATACTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTAGCCACatga                        >  1:5294/1‑149 (MQ=255)
                                                                                                                    ctgctgTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACatgat                       <  7:90646/149‑1 (MQ=255)
                                                                                                                    ctgctgTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACatgatg                      <  4:2600/149‑1 (MQ=255)
                                                                                                                    ctgctgTGGCTTCTGGGTAACAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACatgat                       >  8:471213/1‑149 (MQ=255)
                                                                                                                     tgctgTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGc                               <  7:129811/139‑1 (MQ=255)
                                                                                                                     tgctgTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGc                               >  8:129811/1‑139 (MQ=255)
                                                                                                                       ctgTGGCGTCTGGGTACCGGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACatgatgat                    <  6:80447/149‑1 (MQ=255)
                                                                                                                          tgGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGCGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGa                 <  2:300832/148‑1 (MQ=255)
                                                                                                                            gCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAg                                       <  8:86033/124‑1 (MQ=255)
                                                                                                                            gCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAg                                       >  7:86033/1‑124 (MQ=255)
                                                                                                                            gCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAg                                       >  7:320734/1‑124 (MQ=255)
                                                                                                                            gCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAg                                       <  8:320734/124‑1 (MQ=255)
                                                                                                                              ttCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCTGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGAtttt             >  1:468969/1‑149 (MQ=255)
                                                                                                                              ttCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTg            >  6:314982/1‑149 (MQ=255)
                                                                                                                              ttCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTg            >  5:178153/1‑149 (MQ=255)
                                                                                                                                cTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCTGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGAtttt             <  2:468969/147‑1 (MQ=255)
                                                                                                                                  gggTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGAtttt             >  6:109321/1‑145 (MQ=255)
                                                                                                                                  gggTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGAtttt             <  5:109321/145‑1 (MQ=255)
                                                                                                                                   ggTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTAt       <  5:314982/149‑1 (MQ=255)
                                                                                                                                      aCCAGCCTCTCTTCCCATGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCt     >  8:305985/1‑149 (MQ=255)
                                                                                                                                        cAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGga  <  4:216874/149‑1 (MQ=255)
                                                                                                                                            ctctctTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCt     <  6:439794/143‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                            
TGAAAAAACAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTGGA  >  NC_000913/755190‑755475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑