Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,918,497 G→A 5.1% Q297* (CAA→TAA)  gudD ← D‑glucarate dehydratase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,918,4970GA 5.1% 258.0 / 2.4 78Q297* (CAA→TAA) gudDD‑glucarate dehydratase 1
Reads supporting (aligned to +/- strand):  ref base G (42/32);  new base A (2/2);  total (44/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.01e-01

ATCGAAGTGGTTGTTAGAGTGTGAACCCCAGGTCAGGCCAAATTCATGGCACATTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCTTGCTCCGCACCACACGGATCTTCTGCATAAGCCAGCGAACCTTTCAGGTATTTACC  >  NC_000913/2918351‑2918641
                                                                                                                                                  |                                                                                                                                                
atCGAAGTGGTTGTTAGAGTGTGAACCCCAGGTCAGGCCAAATTCATGGCACATTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTgc                                                                                                                                                 >  2:24425/1‑148 (MQ=255)
 tCGAAGTGGTTGTTAGAGTGTGAACCCCAGGTCAGGCCAAATTCATGGCACATTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTgc                                                                                                                                                 <  2:202775/147‑1 (MQ=255)
  cGAAGTGGTTGTTAGAGTGTGAACCCCAGGTCAGGCCAAATTCATGGCACATTTGCGCCCCACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTgccg                                                                                                                                               >  5:365086/1‑148 (MQ=255)
   gAAGTGGTTGTTAGAGTGTGAACCCCAGGTCAGGCCAAATTCATGGCACATTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCAAGCGGGATAACAACGGATTGCAGGGAGCGCGTATGGCCCATTTgccgc                                                                                                                                              >  6:404694/1‑148 (MQ=255)
                gagTGTGAACCCCAGGTCAGGCCAACTTCATGGCACATTTGCGCCACCCGTACCGAACCTTGCATTGTCCAGAACTGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGa                                                                                                                                 <  7:175781/148‑1 (MQ=255)
                 agTGTGAACCCCAGGTCAGGCCAAATTCATGGCACATTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGAt                                                                                                                                <  4:275504/148‑1 (MQ=255)
                    gtgAACCCCAGGTCAGGCCAAATTCATGGCACATTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATCGCCCATTTGCCGCCAGTCGGTGGCGATCat                                                                                                                             <  1:52874/148‑1 (MQ=255)
                      gAACCCCAGGTCAGGCCAAATTCATGGCACATTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGTCGATCatat                                                                                                                           >  3:298667/1‑148 (MQ=255)
                          cccAGGTCAGGCCAAATTCATGGCACATTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGt                                                                                                                       <  5:142099/148‑1 (MQ=255)
                              ggTCAGGCCAAATTCATGGCACATTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTg                                                                                                                     >  8:1155/1‑146 (MQ=255)
                                tCAGGCCAAATTCATGGCACATTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTACCGCCAGTCGGTGGCGa                                                                                                                                 <  5:372981/132‑1 (MQ=255)
                                tCAGGCCAAATTCATGGCACATTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTACCGCCAGTCGGTGGCGa                                                                                                                                 >  8:40207/1‑132 (MQ=255)
                                tCAGGCCAAATTCATGGCACATTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTACCGCCAGTCGGTGGCGa                                                                                                                                 <  7:40207/132‑1 (MQ=255)
                                tCAGGCCAAATTCATGGCACATTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTACCGCCAGTCGGTGGCGa                                                                                                                                 >  6:372981/1‑132 (MQ=255)
                                     ccAAATTCATGGCACATTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCgg                                                                                                              >  5:403513/1‑146 (MQ=255)
                                     ccAAATTCATGGCACATTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGACAGTCGGTGGCGATCATATTGGTTGCAGTCgg                                                                                                              >  3:81401/1‑146 (MQ=255)
                                      cAAATTCATGGCACATTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAg                                                                                                           <  7:54633/148‑1 (MQ=255)
                                       aacatCATGGCACATTTGCGCCACACGGACCGAACCTTGCATTGGCCAGAAAGGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGa                                                                                                          <  2:217469/144‑1 (MQ=255)
                                          ttCATGGCACATTTGCGCCACACGTACCGCACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCt                                                                                                       >  6:367893/1‑148 (MQ=255)
                                            cATGGCACATTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTg                                                                                                      <  2:223873/147‑1 (MQ=255)
                                              tGGCACATTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGt                                                                                                                 >  4:147862/1‑134 (MQ=255)
                                              tGGCACATTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGt                                                                                                                 <  3:147862/134‑1 (MQ=255)
                                                gCACATTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTcgcg                                                                                                 <  7:201796/148‑1 (MQ=255)
                                                gCACATTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGGCGGTAGACCTGTcgcg                                                                                                 <  3:163423/148‑1 (MQ=255)
                                                   caTTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGa                                                                                                          >  3:27967/1‑136 (MQ=255)
                                                   caTTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGa                                                                                                          <  4:27967/136‑1 (MQ=255)
                                                   caTTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGCGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGa                                                                                              <  5:242639/148‑1 (MQ=255)
                                                    aTTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTgccgcc                                                                                                                                             <  8:137719/100‑1 (MQ=255)
                                                    aTTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTgccgcc                                                                                                                                             >  7:137719/1‑100 (MQ=255)
                                                    aTTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTg                                                                                                                         <  3:93174/120‑1 (MQ=255)
                                                    aTTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTg                                                                                                                         >  4:93174/1‑120 (MQ=255)
                                                         cgcCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGGCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAAc                                                                                        <  4:51363/148‑1 (MQ=255)
                                                               acGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCa                                                                                                                              >  6:161116/1‑104 (MQ=255)
                                                               acGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCa                                                                                                                              <  5:161116/104‑1 (MQ=255)
                                                               acGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGt                                                                                                             >  8:34885/1‑121 (MQ=255)
                                                               acGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGt                                                                                                             <  7:34885/121‑1 (MQ=255)
                                                                cGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCa                                                                                 >  7:357577/1‑148 (MQ=255)
                                                                      gAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACtt                                                                           >  4:136168/1‑148 (MQ=255)
                                                                        aCCTTGCATTGTCCAGAAATGCGTATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCa                                                                         >  5:199666/1‑148 (MQ=255)
                                                                        aCCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTgccgcc                                                                                                                                             <  1:108842/80‑1 (MQ=255)
                                                                        aCCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTgccgcc                                                                                                                                             >  2:108842/1‑80 (MQ=255)
                                                                        aCCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCata                                                                                                                            <  3:270611/97‑1 (MQ=255)
                                                                        aCCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCata                                                                                                                            >  4:270611/1‑97 (MQ=255)
                                                                               aTTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCgg                                                                  <  5:391374/148‑1 (MQ=255)
                                                                                    ccAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTgccg                                                                                                                                               >  7:308893/1‑66 (MQ=255)
                                                                                    ccAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTgccg                                                                                                                                               <  8:308893/66‑1 (MQ=255)
                                                                                     cAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAAc                                                            >  8:215282/1‑148 (MQ=255)
                                                                                       gAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCt                                                          >  3:235357/1‑148 (MQ=255)
                                                                                       gAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCt                                                          >  8:15992/1‑148 (MQ=255)
                                                                                          aTGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGCACTCTGCCATCACTTCACGCCCGGAGCAACCTTGc                                                       >  3:29843/1‑148 (MQ=255)
                                                                                              ggATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCTTGCTCCg                                                   >  7:37808/1‑148 (MQ=255)
                                                                                               gATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCTTGCTCCGc                                                  <  7:1155/148‑1 (MQ=255)
                                                                                                 tCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGCACTCTGCCATCACTTCACGCCCGGAGAAACCTTGCTCCGcac                                                >  7:402981/1‑148 (MQ=255)
                                                                                                  ccGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCTTGCTCCGcac                                                >  3:137889/1‑147 (MQ=255)
                                                                                                    gCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCTTGCTCCGcaccac                                             <  4:298667/148‑1 (MQ=255)
                                                                                                     ccAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCTTGCTCCGCACcaca                                            >  6:5942/1‑148 (MQ=255)
                                                                                                       aGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCAGTGGCGATCATATTGGTTGCAGTCGGTAGCCCTGTCGCGCGACGGAACTCTGCCATCACTTCCCGCCCGGAGAACCCTTGCTCCGcaccccccg                                          >  4:3853/1‑143 (MQ=255)
                                                                                                           ggATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCTTGCTCCGCACCACACGGAtc                                      >  1:352447/1‑148 (MQ=255)
                                                                                                            gATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCTTGCTCCGCACCACACGGAtct                                     <  4:235357/148‑1 (MQ=255)
                                                                                                             atatCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCTTGCTCCGCACCACACGGAtctt                                    <  3:230104/148‑1 (MQ=255)
                                                                                                                tCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCTTGCTCCGCACCACACGGATCTTCTg                                 <  8:402981/148‑1 (MQ=255)
                                                                                                                  aaCGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCCTCACTTCACGCCCGGAGAAACCTTGCTCCGCACCACACGGATCTTCTGCa                               >  2:171518/1‑148 (MQ=255)
                                                                                                                  aaCGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCTTGCTCCGCACCACACGGATCTTCTGCa                               <  5:367893/148‑1 (MQ=255)
                                                                                                                   aCGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCTTGCTCCGCACCACACGGATCTTCTGCa                               >  2:179669/1‑147 (MQ=255)
                                                                                                                    cGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCTTGCTCCGCACCACACGGATCTTCTGCAt                              >  1:433590/1‑147 (MQ=255)
                                                                                                                       aTTGAAGGGAGAGCGTATGGCCCATTTGCCGCAAGTCGGTGGCGATCATATTGGATGCAGTCGGGAGACCTGTAGCGCGACGGAACTATGCAATCACTTCACGCCCGGAGAAACCTTTCTCCGCACAACACGGATCATCTGCAGAAGc                          >  6:122899/1‑148 (MQ=255)
                                                                                                                        ttGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCTTGCTCCGCACCACACGGATCTTCTGCATAAGcc                         <  1:179669/148‑1 (MQ=255)
                                                                                                                          gCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAACCCTTGCTCCGCACCACACGGATCTTCTGCATAAGCCAg                       >  5:235981/1‑148 (MQ=255)
                                                                                                                              ggAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCTTGCTCCGCACCACACGGATCTTCTGCATAAGCCAGCGaa                   <  5:346556/148‑1 (MQ=255)
                                                                                                                               gagaGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCTTGCTCCGCACCACACGGATCTTCTGCATAAGCCAGCGAAc                  >  3:204679/1‑148 (MQ=255)
                                                                                                                               gagaGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCTTGCTCCGCACCACACGGATCTTCTGCATAAGCCAGCGAAc                  >  3:356547/1‑148 (MQ=255)
                                                                                                                               gagaGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCTTGCTCCGCACCACACGGATCTTCTGCATAAGCCAGCGAAc                  <  3:136168/148‑1 (MQ=255)
                                                                                                                                 gagCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCTTGCTCCGCACCACACGGATCTTCTGCATAAGCCAGCGAACCt                >  7:251439/1‑148 (MQ=255)
                                                                                                                                   gCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCTTGCTCCGCACCACACGGATCTTCTGCATAAGCCAGCGAACCttt              >  5:280763/1‑148 (MQ=255)
                                                                                                                                   gCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCTTGCTCCGCACCACACGGATCTTCTGCATAAGCCAGCGAACCttt              <  7:15992/148‑1 (MQ=255)
                                                                                                                                       aTGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCTTGCTCCGCACCACACGGATCTTCTGCATAAGCCAGCGAACCTTTCAgg          >  6:378190/1‑148 (MQ=255)
                                                                                                                                        tGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGGCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCTTGCTCCGCACCACACGGATCTTCTGCATAAGCCAGCGAACCTTTCAGGt         <  5:277485/148‑1 (MQ=255)
                                                                                                                                         ggCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCTTGCTCCGCACCACACGGATCTTCTGCATAAGcc                         <  6:3090/131‑1 (MQ=255)
                                                                                                                                         ggCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCTTGCTCCGCACCACACGGATCTTCTGCATAAGcc                         >  5:3090/1‑131 (MQ=255)
                                                                                                                                          gCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCTTGCTCCGCACCACACGGATCTTCTGCATAAGCCAGCGAACCTTTCAGGTAt       >  8:164773/1‑148 (MQ=255)
                                                                                                                                          gCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCTTGCTCCGCACCACACGGATCTTCTGCATAAGCCAGCGAACCTTTCAGGTAt       <  8:251439/148‑1 (MQ=255)
                                                                                                                                           cccATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCTTGCTCCGCACCACACGGCTCTTCTGCATAAGCCAGCGAACCTTTCAGGAAtt      >  7:397675/1‑148 (MQ=255)
                                                                                                                                             cATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCGGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCTTGCTCCGCACCACACGGATCTTCTGCATAAGCCAGCGAACCTTTCAGGTATTTa    >  3:131778/1‑148 (MQ=255)
                                                                                                                                             cATTTGCCGCCAGTCGGGGGCGATCATTTTGGTTGCAGTCTGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCTTGCTCCGCACCACACGGATCTTCTGCATAAGCCAGCCAACCTTTCAGGTATTTa    <  8:397675/148‑1 (MQ=255)
                                                                                                                                              aTTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCTTCACTTCACGCCCGGAGAACCCTTGCTCCGCACCCCCCGGATCTTCTGCATAAGCCAGCGAACCTTTCAGGTATTTAc   >  7:398160/1‑148 (MQ=255)
                                                                                                                                               tttGCCGCCAGTCTGAGGCGATCATATTGGTTGCAGTCGGTAGACCTGACGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCTTGCTCCGCACCACACGGATCATCTGCATAAGCCAGCGAACCTTTCAGGTATTTAcc  >  2:254938/1‑148 (MQ=255)
                                                                                                                                                ttGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCTTGCTCCGCACCACACGGATCTTCTGCATAAGCCAGCGAACCTTTCAGGTATTTAcc  >  3:48569/1‑147 (MQ=255)
                                                                                                                                                ttGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCTTGCTCCGCACCACACGGATCTTCTGCATAAGCCAGCGAACCTTTCAGGTATTTAcc  >  5:194865/1‑147 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
ATCGAAGTGGTTGTTAGAGTGTGAACCCCAGGTCAGGCCAAATTCATGGCACATTTGCGCCACACGTACCGAACCTTGCATTGTCCAGAAATGCGGATCCGCCAGCGGGATATCAACGGATTGCAGGGAGAGCGTATGGCCCATTTGCCGCCAGTCGGTGGCGATCATATTGGTTGCAGTCGGTAGACCTGTCGCGCGACGGAACTCTGCCATCACTTCACGCCCGGAGAAACCTTGCTCCGCACCACACGGATCTTCTGCATAAGCCAGCGAACCTTTCAGGTATTTACC  >  NC_000913/2918351‑2918641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑