Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,220,0090GT35.3% 47.8 / 27.1 34V472F (GTT→TTT) aceKisocitrate dehydrogenase kinase/phosphatase
Reads supporting (aligned to +/- strand):  ref base G (13/9);  new base T (5/7);  total (18/16)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCACCCGTCACGGGCGTGTGGTTTTTTATGATTACGATGAAATTTGCTACATGACGGAAGTGAATTTCCGCGACATCCCGCCGCCGCGCTATCCGGAAGACGAACTTGCCAGCGAACCGTGGTACAGCGTCTCGCCGGGCGATGTTTTCCCGGAAGAGTTTCGCCACTG  >  NC_000913/4219862‑4220157
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aaTATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCACCCGTCACGGGCGTGTGGt                                                                                                                                                     >  8:126667/1‑149 (MQ=255)
   atCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCACCCGTCACGGGCGTGTGGtttt                                                                                                                                                  >  2:85595/1‑149 (MQ=255)
        gctggAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCACCCGTCACGGGCGTGTGTTTTTTTa                                                                                                                                               <  5:68268/147‑1 (MQ=255)
        gctggAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCACCCGTCACGGGCGTGTGTTTTTTTATg                                                                                                                                             <  5:128065/149‑1 (MQ=255)
         ctggAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCACCCGTCACGGGCGTGTGGTTTTTTATGa                                                                                                                                            <  4:81372/149‑1 (MQ=255)
                         gTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCACCCGTCACGGGCGTGTGGTTTTTTATGATTAGGATGAAATTTGc                                                                                                                            <  1:85595/149‑1 (MQ=255)
                                                    aaTACGGTAACGCTATTCGCCAGCGTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCACCCGTCACGGGCGTGTGTTTTTTTATGATTACGATGAAATTTGCTACATGACGGAAGTGAATTTCCGCGAc                                                                                                 <  4:119608/149‑1 (MQ=255)
                                                      tACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCACCCGTCACGGGCGTGTGGTTTTTTATGATTACGATGAAATTTGCTACATGACGGAAGTGAATTTCCGCGACAt                                                                                               >  1:165790/1‑149 (MQ=255)
                                                             aCGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCACCCGTCACGGGCGTGTGGTTTTTTATGATTACGATGAAATTTGCTACATGACGGAAGTGAATTTCCGCGACATCccgccg                                                                                        <  2:165790/149‑1 (MQ=255)
                                                              cGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCACCCGTCACGGGCGTGTGGTTTTTTATGATTACGATGAAATTTGCTACATGACGGAAGTGAATTTCCGCGACATCccgccgc                                                                                       >  1:57954/1‑149 (MQ=255)
                                                                      gCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCACCCGTCACGGGCGTGTGTTTTTTTATGATTACGATGAAATTTGCTACATGACGGAAGTGAATTTCCGCGACATCCCGCCGCCGCGCTAt                                                                               >  2:102830/1‑149 (MQ=255)
                                                                                cGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCACCCGTCACGGGCGTATGGTTTTTTATGATTACGATGAAATTTGCTACATGACGGAAGTGAATTTCCGCGACATCCCGCCGCCGCGCTATCCGGAAGAc                                                                      >  6:88978/1‑148 (MQ=255)
                                                                                       aaCATTTTCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCACCCGTCACGGGCGTGTGGTTTTTTATGATTACGa                                                                                                                                      >  6:113266/1‑77 (MQ=255)
                                                                                       aaCATTTTCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCACCCGTCACGGGCGTGTGGTTTTTTATGATTACGa                                                                                                                                      <  5:113266/77‑1 (MQ=255)
                                                                                           ttttCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCACCCGTCACGGGCGTGTGTTTTTTTATGATTACGATGAAATTTGCTACATGACGGAAGTGAATTTCCGCGACATCCCGCCGCCGCGCTATCCGGAAGACGAACTTGCCAGc                                                          >  5:170141/1‑149 (MQ=255)
                                                                                                   ggCGACATGCTGTTTAAAAACTTCGGTGTCACCCGTCACGGGCGTGTGGTTTTTTATGATTACGATGAAATTTGCTACATGACGGAAGTGAATTTCCGCGACATCCCGCCGCCGCGCTATCCGGAAGACGAACTTGCCAGCGAACCGTg                                                  >  5:153164/1‑149 (MQ=255)
                                                                                                    gCGACATGCTGTTTAAAAACTTCGGTGTCACCCGTCACGGGCGTGTGTTTTTTTATGATTACGATGAAATTTGCTACATGACGGAAGTGAATTTCCGCGACATCCCGCCGCCGCGCTATCCGGAAGACGAACTAGCCAGCGAACCGAgg                                                 >  8:7869/1‑149 (MQ=255)
                                                                                                    gCGACATGCTGTTTAAAAACTTCGGTGTCACCCGTCACGGGCGTGTGGTTTTTTATGATTACGATGAAATTTGCTACATGACGGAAGTGAATTTCCGCGACATCCCGCCGCCGCGCTATCCGGAAGACGAACTTGCCAGCGAACCGTg                                                  <  6:153164/148‑1 (MQ=255)
                                                                                                      gACATGCTGTTTAAAAACTTCGGTGTCACCCGTCACGGGCGTGTGGTTTTTTATGATTACGATGAAATTTGCTACATGACGGAAGTGAATTTCCGCGACATCCCGCCGCCGCGCTATCCGGAAGACGAACTTGCCAGCGAACCGTGGTa                                               >  2:174346/1‑149 (MQ=255)
                                                                                                      gACATGCTGTTTAAAAACTTCGGTGTCACCCGTCACGGGCGTGTGGTTTTTTATGATTACGATGAAATTTGCTACATGACGGAAGTGAATTTCCGCGACATCCCGCCGCCGCGCTATCCGGAAGACGAACTTGCCAGCGAACCGTGGTa                                               >  5:162706/1‑149 (MQ=255)
                                                                                                         aTGCTGTTTAAAAACTTCGGTGTCACCCGTCACGGGCGTGTGGTTTTTTATGATTACGATGAAATTTGCTACATGACGGAAGTGAATTTCCGCGACATCCCGCCGCCGCGCTATCCGGAAGACGAACTTGCCAGCGAACCGt                                                   >  5:123557/1‑142 (MQ=255)
                                                                                                          tGCTGTTTAAAAACTTCGGTGTCACCCGTCACGGGCGTGTGGTTTTTTATGATTACGATGAAATTTGCTACATGACGGAAGTGAATTTCCGCGACATCCCGCCGCCGCGCTATCCGGAAGACGAACTTGCCAGCGAACCGTGGTACAGc                                           <  7:126667/149‑1 (MQ=255)
                                                                                                              gTTTAAAAACTTCGGTGTCACCCGTCACGGGCGTGTGTTTTTTTATGATTACGATGAAATTTGCTACAt                                                                                                                       <  2:133974/69‑1 (MQ=255)
                                                                                                              gTTTAAAAACTTCGGTGTCACCCGTCACGGGCGTGTGTTTTTTTATGATTACGATGAAATTTGCTACAt                                                                                                                       >  1:133974/1‑69 (MQ=255)
                                                                                                               tttAAAAACTTCGGTGTCACCCGTCACGGGCGTGTGTTTTTTTATGATTACGATGAAATTTGCTACATGACGGAAGTGAATTTCCGCGACATCCCGCCGCCGCGCTATCCGGAAGACGAACTTGCCAGCGAACCGTGGTACAACGtc                                        <  6:170141/147‑1 (MQ=255)
                                                                                                                 tAAAAACTTCGGTGTCACCCGTCACGGGCGTGTGGTTTTTTATGATTACGATGAAATTTGCTACATGACGGAAGTGAATTTCCGCGACATCCCGCCGCCGCGCTATCCGGAAGACGAAc                                                                  <  2:31175/119‑1 (MQ=255)
                                                                                                                 tAAAAACTTCGGTGTCACCCGTCACGGGCGTGTGGTTTTTTATGATTAAGATGAAATTTGCTACATGACGGAAGTGAATTTCCGCGACATCCCGCCGCCGCGCTATCCGGAAGACGAAc                                                                  >  1:31175/1‑119 (MQ=255)
                                                                                                                  aaaaaCTTCGGTGTCACCCGTCACGGGCGTGTGTTTTTTTATGATTACGATGAAATTTGCTACATGACGGAAGTGAATTTCCGCGACATCCCGCCGCCGCGCTATc                                                                              <  2:152529/106‑1 (MQ=255)
                                                                                                                  aaaaaCTTCGGTGTCACCCGTCACGGGCGTGTGTTTTTTTATGATTACGATGAAATTTGCTACATGACGGAAGTGAATTTCCGCGACATCCCGCCGCCGCGCTATc                                                                              >  1:152529/1‑106 (MQ=255)
                                                                                                                                  cccGTCACGGGCGTGTGGTTTTTTATGATTACGATGAAATTTGCTACATGACGGAAGTGAATTTCCGCGACATCCCGCCGCCGCGCTATCCGGAAGACGAACTTGCCAGCGAACCGTgg                                                 >  6:168297/1‑119 (MQ=255)
                                                                                                                                           ggCGTGTGGTTTTTTATGATTACGATGAAATTTGCTACATGACGGAAGTGAATTTCCGCGACATCCCGCCGCCGCGCTATCCGGAAGACGAACTTGCCAGCGAACCGTGGTACAGCGTCTCGCCGGGCGATGTTTTCCCGGAAGAGttt          <  6:162706/149‑1 (MQ=255)
                                                                                                                                            gCGTGTGTTTTTTTATGATTACGATGAAATTTGCTACATGACGGAAGTGAATTTCCGCGACATCCCGCCGCCGCGCTATCCGGAAGACGAACTTGCCAGCGAACCGTGGTACAGCGTCTCGCCGGGCGATGTTTTCCCGGAAGAGTTTc         <  1:102830/149‑1 (MQ=255)
                                                                                                                                              gtgtGGTTTTTTATGATTACGATGAAATTTGCTACATGACGGAAGTGAATTTCCGCGACATCCCGCCGCCGCGCTATCCGGAAGACGAACTTGCCAGCGAACCGTGGTACAGCGTCTCGCCGGGCGATGTTTTCCCGGAAGAGTTTCGc       <  1:174346/149‑1 (MQ=255)
                                                                                                                                                gtgGTTTTTTATGATTACGATGAAATTTGCTACATGACGGAAGTGAATTTCCGCGACATCCCGCCGCCGCGCTATCCGGAAGACGAACTTGCCAGCGAACCGTGGTACAGCGTCTCGCCGGGCGATGTTTTCCCGGAAGAGTTTCGCCa     >  2:43800/1‑149 (MQ=255)
                                                                                                                                                   gTTTTTTATGATTACGATGAAATTTGCTACATGACGGAAGTGAATTTCCGCGACATCCCGCCGCCGCGCTATCCGGAAGACGAACTTGCCAGCGAACCGTGGTACAGCGTCTCGCCGGGCGATGTTTTCCCGGAAGAGTTTCGCCACTg  >  6:1225/1‑149 (MQ=255)
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AATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCACCCGTCACGGGCGTGTGGTTTTTTATGATTACGATGAAATTTGCTACATGACGGAAGTGAATTTCCGCGACATCCCGCCGCCGCGCTATCCGGAAGACGAACTTGCCAGCGAACCGTGGTACAGCGTCTCGCCGGGCGATGTTTTCCCGGAAGAGTTTCGCCACTG  >  NC_000913/4219862‑4220157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑