Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 578,809 T→C 100% T29A (ACA→GCA)  borD ← DLP12 prophage; putative lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913578,8090TC98.6% 10691.6 / 37.8 3778T29A (ACA→GCA) borDDLP12 prophage; putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (29/27);  new base C (1676/2012);  total (1728/2050)
Fisher's exact test for biased strand distribution p-value = 3.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.53e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 3814 total aligned reads displayed.

ATCCATTTACGAATGTTTGCTGGGTTTCTGTTTTAACAACATTTTCTGCGCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAAC  >  NC_000913/578665‑578954
                                                                                                                                                |                                                                                                                                                 
   cATTTACGAATGTTTGCTGGGTTTCTGTTTTAACAACATTTTCTGCGCCGCCACAAATTTTAGATGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCggtttg                                                                                                                                            >  1:36413/1‑149 (MQ=255)
    aTTTACGAATGTTTGCTGGGTTTCTGTTTTAACAACATTTTCTGCGCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCggtttgt                                                                                                                                           >  6:302981/1‑149 (MQ=255)
    aTTTACGAATGTTTGCTGGGTTTCTGTTTTAACAACATTTTCTGCGCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCAAGAAACGAATAAATGATGGTTGATGGTTTCCTTTGGTGCTACTGCTTCCggtttgt                                                                                                                                           >  4:251029/1‑149 (MQ=255)
     tttACGAATGTTTGCTGGGTTTCTGTTTTAACAACATTTTCTGCGCCTCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCggtttgtt                                                                                                                                          <  3:308337/149‑1 (MQ=255)
     tttACGAATGTTTGCTGGGTTTCTGTTTTAACAACATTTTCTGCGCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCggtttgtt                                                                                                                                          >  7:114855/1‑149 (MQ=255)
     tttACGAATGTTTGCTGGGTTTCTGTTTTAACAACATTTTCTGCGCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCggtttgtt                                                                                                                                          <  1:312354/149‑1 (MQ=255)
        aCGAATGTTTGCTGGGTTTCTGTTTTAACAACATTTTCTGCGCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGtttt                                                                                                                                        <  7:334649/148‑1 (MQ=255)
         cGAATGTTTGCTGGGTTTCTGTTTTAACAACATTTTCTGCGCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGa                                                                                                                                      <  4:52960/149‑1 (MQ=255)
            aTGTTTGCTGGGTTTCTGTTTTAACAACATTTTCTGCGCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACa                                                                                                                                   <  2:67073/149‑1 (MQ=255)
             tGTTTGCTGGGTTTCTGTTTTAACAACATTTCCTGCGCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAg                                                                                                                                  <  1:250013/149‑1 (MQ=255)
               tttGCTGGGTTTCTGTTTTAACAACATTTTCTGCGCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGt                                                                                                                                 >  5:281986/1‑148 (MQ=255)
                   cTGGGTTTCTGTTTTAACAACATTTTCTGCGCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTaaac                                                                                                                             <  7:82199/148‑2 (MQ=255)
                   cTGGGTTTCTGTTTTAACAACATTTTCTGCGCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTGTTGAACAGTacacg                                                                                                                            >  2:309056/1‑145 (MQ=255)
                    tGGGTTTCTGTTTTAACAACATTTTCTGCGCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTaa                                                                                                                               <  6:161295/145‑1 (MQ=255)
                    tGGGTTTCTGTTTTAACAACATTTTCTGCGCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGt                                                                                                                           >  7:284476/1‑149 (MQ=255)
                    tGGGTTTCTGTTTTAACAACATTTTCTGCGCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGACCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGATGCCGGTTTGTTTTGAACAGTAAACGt                                                                                                                           >  7:2195/1‑149 (MQ=255)
                       ttttCTGGTTTATCAACATTTTCTGCGCATACACAAATTTTAGCTGCTTCGACAGTTTTCTTGTGCCCATTTCCATATACGTAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACa                                                                                                                                   <  5:341867/137‑1 (MQ=255)
                       gTTTCTGTTTTAACAACATTTTCTGCGCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTg                                                                                                                        <  7:141562/149‑1 (MQ=255)
                          tCTGTTTTAACAACATTTTCTGCGCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGtt                                                                                                                      <  5:307134/148‑1 (MQ=255)
                              ttttAACAACATTTTCTGCGCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGAGATGGTTTCCTTTGGTGCTACTGCTGACGGTTTGTTTTGAACAGTAAACGTCTGTTGAGca                                                                                                                 >  1:170362/1‑149 (MQ=255)
                                ttAACAACATTTTCTGCGCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGcac                                                                                                                <  3:15284/148‑1 (MQ=255)
                                ttAACAACATTTTCTGCGCCGCCACAAATTTTAGCTGCATCGACAGTTTTCATCTGCCCAATTCCAGAAAAGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGcaca                                                                                                               >  1:144259/1‑149 (MQ=255)
                                  aacaacATTTTCTGCGCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCggttt                                                                                                                                             >  6:130047/1‑117 (MQ=255)
                                  aacaacATTTTCTGCGCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATc                                                                                                             >  2:22960/1‑149 (MQ=255)
                                  aacaacATTTTCTGCGCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAAGGCTGGGTGATGGTTTCCTTTGGTGCTAGTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCCCATc                                                                                                             >  8:103635/1‑149 (MQ=255)
                                   acaacaTTTTCTGCGCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCggtttgtt                                                                                                                                          >  8:136880/1‑119 (MQ=255)
                                    caacaTTTTCTGCGCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCt                                                                                                           <  4:142498/149‑1 (MQ=255)
                                    caacaTTTTCTGCGCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCt                                                                                                           >  2:97602/1‑149 (MQ=255)
                                     aacaTTTTCTGCGCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGgtttgttt                                                                                                                                         >  5:254973/1‑118 (MQ=255)
                                     aacaTTTTCTGCGCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTg                                                                                                          >  7:248016/1‑149 (MQ=255)
                                     aacaTTTTCTGCGCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTg                                                                                                          >  7:47020/1‑149 (MQ=255)
                                     aacaTTTTCTGCGCCGCCACAAATTTTAGCTGCATCGAAAGTAATCTACTGCCCAAATCCAGAAACGAAGAAAAGATGGTTGAAGGTGACCTTAGGGGCTACTTCTGCCCGATTGTTTAGAACAGTAAACGTCTGTTGAGcacagatcc                                                                                                          >  5:226996/1‑144 (MQ=255)
                                      acaTTTTCTGCGCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAATCGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTATACGTCTGTTGAGCACATCCCGt                                                                                                         <  1:28035/149‑1 (MQ=255)
                                      acaTTTTCTGCGCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGt                                                                                                         <  3:126361/149‑1 (MQ=255)
                                      acaTTTTCTGCGCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGt                                                                                                         <  1:188580/149‑1 (MQ=255)
                                         ttttCTGCGCCGCCACAAATTTTAGCTGCATCGCCAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGGTTGTTTTGAACAGTAAACGTCTGTTGAGCGCATCCTGtaat                                                                                                      <  2:365206/149‑1 (MQ=255)
                                            tCTGCGCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCACAAACGAAGAAATGATGGGTGATGGGTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGtaataa                                                                                                    <  8:166745/148‑1 (MQ=255)
                                             cTGCGCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAg                                                                                                   <  4:112861/148‑1 (MQ=255)
                                               gcgcCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAg                                                                                                >  7:162634/1‑149 (MQ=255)
                                                cgccgcCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAg                                                                                                <  7:311620/148‑1 (MQ=255)
                                                cgccgcCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAg                                                                                                <  5:202209/148‑1 (MQ=255)
                                                  ccgccACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGc                                                                                             <  2:337885/149‑1 (MQ=255)
                                                    gccACAAATTTTAGTTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGTGCCa                                                                                           >  6:103593/1‑149 (MQ=255)
                                                       acaAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAgcg                                                                                        >  4:168973/1‑149 (MQ=255)
                                                         aaaTTTTAGCTGCATCGACAGTTTTCTTCTGCCCTATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAgag                                                                                        >  2:25522/1‑145 (MQ=255)
                                                          aaTTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAg                                                                                     <  1:223847/149‑1 (MQ=255)
                                                            ttttAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTa                                                                                   <  3:85524/149‑1 (MQ=255)
                                                               tAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGc                                                                                 <  4:19893/148‑1 (MQ=255)
                                                               tAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGc                                                                                 <  6:249471/148‑1 (MQ=255)
                                                                 gCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGa                                                                               <  2:14826/148‑1 (MQ=255)
                                                                   tGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCa                                                                                                 >  3:54578/1‑128 (MQ=255)
                                                                    gCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTg                                                                                                                                       >  7:190357/1‑89 (MQ=255)
                                                                     cATCGACATTTTTATTCTGCCAAATTCCAGAAACGAAGAAATGATTGGTGATTGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTa                                                                            <  4:361833/147‑1 (MQ=255)
                                                                     cATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGa                                                                                                                    >  4:89310/1‑107 (MQ=255)
                                                                         gACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAg                                                                                     >  1:339450/1‑134 (MQ=255)
                                                                          aCAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTTTTTTGAACAGTAAACGTCTTTTGAGAACATCCTGTAATAAGAAGGGACAGCGCAGTAGCGAGTAGCAtttt                                                                     >  5:5325/1‑149 (MQ=255)
                                                                          aCAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCAtttt                                                                     >  8:7535/1‑149 (MQ=255)
                                                                          aCAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCAtttt                                                                     >  4:278073/1‑149 (MQ=255)
                                                                           cAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCa                                                                         >  1:74576/1‑144 (MQ=255)
                                                                            aGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGtttt                                                                                                                                        >  1:100248/1‑80 (MQ=255)
                                                                            aGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGc                                                                                 >  8:1938/1‑135 (MQ=255)
                                                                            aGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCAtttttt                                                                   <  8:34884/149‑1 (MQ=255)
                                                                              ttttCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTc                                                                 <  3:182663/149‑1 (MQ=255)
                                                                                 tctgctGCCCAATTCCAGAACCGAAGAAATGATGTGTGAGGGTTTCGTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCTCATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCAt                                                               <  3:203382/148‑1 (MQ=255)
                                                                                  cttctGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCATg                                                              <  8:25312/148‑1 (MQ=255)
                                                                                  cttctGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCATg                                                              <  5:175487/148‑1 (MQ=255)
                                                                                   ttctGCCCATTTGCAGATACGAAGATATGATGGGTTATGGTTTCCTTTGGTTCTACTGCTGCCGGTTTGTTTTGAACAGTATAGGTCTGTTGAGCACATCCTGTAATAAGCATTGCCAGCGCAGTAGCGAGTAGCATTTTTTTCATGgt                                                            <  1:121107/149‑1 (MQ=255)
                                                                                    tctGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCATGgtg                                                           >  2:397594/1‑149 (MQ=255)
                                                                                     ctGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCATGgt                                                            <  2:104605/147‑1 (MQ=255)
                                                                                      tGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCATGgtgt                                                          >  6:217854/1‑148 (MQ=255)
                                                                                           aaatCCAGAACCGAAGAAATGAGGGGTGATGGGTTCCTTTGGTGCTACTGCTGCCGGGTGGTTTTGAACAGTAAACGGCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGAAGCGAGTAGCATTTTTTTCATGGTGTTATTcc                                                    <  2:173045/146‑1 (MQ=255)
                                                                                           aaTTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCATGGTGTTATTcc                                                    >  2:199328/1‑149 (MQ=255)
                                                                                           aaTTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCATGGTGTTATTcc                                                    >  5:142503/1‑149 (MQ=255)
                                                                                               ccAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCATGGTGTTATTCCCGa                                                 <  8:377204/148‑1 (MQ=255)
                                                                                                 aGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCATGGTGTTATTCCCGATGc                                              >  7:382791/1‑149 (MQ=255)
                                                                                                      cGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCATGGTGTTATTCCCGATGCttttt                                         <  3:252227/149‑1 (MQ=255)
                                                                                                       gaagaaATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTGGCATTTTTTTCATGGTGTTATTCCCGATGCttttt                                         >  4:305486/1‑148 (MQ=255)
                                                                                                        aagaaATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCATGGTGTTATTCCCGATGCTTTTTGa                                       <  8:44700/149‑1 (MQ=255)
                                                                                                         agaaATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCATGGTGTTATTCCCGATGCTTTTTGaa                                      <  6:188801/149‑1 (MQ=255)
                                                                                                          gaaATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTAATGGTGTTATTCCCGATGCTTTTTGAAg                                     >  1:40969/1‑149 (MQ=255)
                                                                                                           aaaTGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCATGGTGTTATTCCCGATGCTTTTTGAAGt                                    <  8:315194/149‑1 (MQ=255)
                                                                                                             atgatgGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTc                                  <  6:367748/149‑1 (MQ=255)
                                                                                                                atgGGTCATGGGTTCCTTTGGTGCTACTGCTGCCGGTTTGTTATGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCTCAGTAGCGAGTAGCATTTTTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGc                                <  2:123301/148‑1 (MQ=255)
                                                                                                                   agTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCAtttttt                                                                   <  7:355802/109‑1 (MQ=255)
                                                                                                                     tGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATc                          <  5:370103/149‑1 (MQ=255)
                                                                                                                          gTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTAt                      <  5:111043/148‑1 (MQ=255)
                                                                                                                          gTTGCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACTTCTGTTGAGCACATCCTGTAATAAGCAGGGCCATCGCAGTAGCGAGTAGCATTTTTTTCTTGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTAt                      <  6:337479/148‑1 (MQ=255)
                                                                                                                            ttCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTAtgtg                   >  3:335754/1‑149 (MQ=255)
                                                                                                                             tCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTAtgtg                   <  6:275601/148‑1 (MQ=255)
                                                                                                                             tCCTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTAtgtg                   <  8:289556/148‑1 (MQ=255)
                                                                                                                               cTTTGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAg                <  8:218987/149‑1 (MQ=255)
                                                                                                                                tttGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGAGCAGTAGCGAGTAGCATTTTTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAg                >  4:335632/1‑148 (MQ=255)
                                                                                                                                 ttGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTa                       >  3:234300/1‑140 (MQ=255)
                                                                                                                                 ttGGTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGa               >  5:78141/1‑148 (MQ=255)
                                                                                                                                   ggTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTACCGAGTAGCATTTTTTTCATGGTGTTATTCCCGAGGCTGTTTGAAGTTCGCAGAATCGTATGTGTAGaaaa            >  2:280830/1‑149 (MQ=255)
                                                                                                                                    gTGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCATCGCAGTAGCGAGTAGCATTTTTTTCATGGTCTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAAt           >  3:359406/1‑149 (MQ=255)
                                                                                                                                     tGCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCATGGTGTTATTCCCGATGCTTTTTCAAGTTCGCAGAATCGTATGTGTAGAAAAt           <  6:86993/148‑1 (MQ=255)
                                                                                                                                      gCTACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTa         <  6:53518/149‑1 (MQ=255)
                                                                                                                                        tACTGCTGCCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTaaa       >  3:282204/1‑149 (MQ=255)
                                                                                                                                           tgctgcCGGTTTGTTTTGAACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCATGGTGTTATTCCCGAAGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTtaaga     >  4:226174/1‑146 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
ATCCATTTACGAATGTTTGCTGGGTTTCTGTTTTAACAACATTTTCTGCGCCGCCACAAATTTTAGCTGCATCGACAGTTTTCTTCTGCCCAATTCCAGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGTCTGTTTGTTTTGAACAGTAAATGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAAC  >  NC_000913/578665‑578954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑