Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 262,978 A→T 6.1% intergenic (+32/+172) thrW → / ← ykfN tRNA‑Thr/pseudogene, YdiA family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913262,9780AT 6.1% 202.7 / 2.3 75intergenic (+32/+172)thrW/ykfNtRNA‑Thr/pseudogene, YdiA family
Reads supporting (aligned to +/- strand):  ref base A (32/38);  new base T (2/3);  total (34/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

TTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCC  >  NC_000913/262830‑263125
                                                                                                                                                    |                                                                                                                                                   
ttGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCa                                                                                                                                                     >  1:120791/1‑149 (MQ=255)
ttGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCa                                                                                                                                                     >  5:18255/1‑149 (MQ=255)
  gTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCa                                                                                                                                                     <  1:165370/147‑1 (MQ=255)
  gTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCa                                                                                                                                                     <  7:181307/147‑1 (MQ=255)
  gTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCa                                                                                                                                                     <  6:80594/147‑1 (MQ=255)
      aCCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTcc                                                                                                                                               >  5:308077/1‑149 (MQ=255)
      aCCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTcc                                                                                                                                               >  1:333187/1‑149 (MQ=255)
        cGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCcca                                                                                                                                             <  6:254413/149‑1 (MQ=255)
         gTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCccac                                                                                                                                            <  8:135909/149‑1 (MQ=255)
             ttCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCa                                                                                                                                                     <  2:186840/136‑1 (MQ=255)
             ttCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCa                                                                                                                                                     >  1:186840/1‑136 (MQ=255)
              tCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCaaa                                                                                                                                        >  8:171980/1‑148 (MQ=255)
                  gTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAAtt                                                                                                                                   <  1:98108/149‑1 (MQ=255)
                  gTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCACCaaaaaaat                                                                                                                                   >  1:334112/1‑146 (MQ=255)
                  gTTCGTCAACATGTCCTTTCTGGGCCTATATACCTCAGTTGGTCGAGCTGGTCATTCTTAATGCGAAGGTCGTTGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTCCGTAAGATCTTATCTTTCTCCCACCAAAAAAtt                                                                                                                                   <  4:331159/149‑1 (MQ=25)
                   ttCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTa                                                                                                                                  <  3:261138/149‑1 (MQ=255)
                      gTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATc                                                                                                                                <  2:217334/148‑1 (MQ=255)
                      gTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATc                                                                                                                                <  3:269161/148‑1 (MQ=255)
                      gTGAACATGGCCTTTCAGGTCCGATATAGCTCCGTTGGTAGAGCAGCGCAATAGTAATGCGAAGGTCGTAGGGTCGACTCCTATTATCGGAACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAAttcgc                                                                                                                                <  8:188803/148‑4 (MQ=37)
                               tCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCTTTCTCCCACCAAAAAATTATc                                                                                                                                <  4:139314/139‑1 (MQ=255)
                               tCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCTTTCTCCCACCAAAAAATTATc                                                                                                                                <  4:133841/139‑1 (MQ=255)
                               tCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCTTTCTCCCACCAAAAAATTATc                                                                                                                                >  3:133841/1‑139 (MQ=255)
                               tCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCTTTCTCCCACCAAAAAATTATc                                                                                                                                >  3:139314/1‑139 (MQ=255)
                               tCCTTTCAGGGCCGATATAGCTCAGTAGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTaa                                                                                                                       <  1:328680/148‑1 (MQ=255)
                                 cTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACa                                                                                                                     <  7:103755/148‑1 (MQ=255)
                                 cTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAg                                                                                                                    >  3:361574/1‑149 (MQ=255)
                                 cTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAg                                                                                                                    >  8:257764/1‑149 (MQ=255)
                                 cTTTCAGGGCCGATATAGCTCAGTTGGTAGACCAGCGCATTCGTATAGCGAAGCTCGTAGGTTCGAGACCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTAATCATTCTCGCACCAAAAAAATATCTTAATGTAACAg                                                                                                                    >  5:291828/1‑149 (MQ=37)
                                        ggCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTCTCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGgtgt                                                                                                             <  6:326323/149‑1 (MQ=255)
                                             atatAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTAGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTgtaagt                                                                                                         >  1:344957/1‑148 (MQ=255)
                                              tataGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTgtaagta                                                                                                        >  7:136484/1‑148 (MQ=255)
                                              tataGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTgtaagta                                                                                                        >  5:97654/1‑148 (MQ=255)
                                                taGCTCAGTTGGTAGAGCATCGCATTCGTAATGCGAAGGTATTAGTTTAGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACTTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTaaa                                                                                                      <  5:165978/148‑1 (MQ=37)
                                                 aGCACAGATGCTACAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTACGACACCAAAAAACCCCACCATTAAAATCAAATTGTTACGTAAGATCATATAATTCTACCAACAAAAAATTAACTAAATGTAACAGCAGGTGTAAGTAAAtt                                                                                                    >  8:110831/1‑149 (MQ=21)
                                                  gCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAAtt                                                                                                    <  2:261252/148‑1 (MQ=255)
                                                    tCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTa                                                                                                 >  3:308751/1‑149 (MQ=255)
                                                      atttGGTAGATCATGGCATTCGTTATTCGTATGTAGTTCGGTCGTATCCTATTATCGGCACCATTTAAATTAAATTTGTTCGTAATTTCTTATTATTCACCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTAt                                                                                                <  2:334112/146‑1 (MQ=255)
                                                      aGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTAt                                                                                                <  7:110831/148‑1 (MQ=255)
                                                        ttGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCaa                                                                                             >  4:141777/1‑149 (MQ=255)
                                                              gagCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAg                                                                                        <  8:263785/148‑1 (MQ=255)
                                                               agcagcGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGAt                                                                                      <  8:136484/149‑1 (MQ=255)
                                                                       aTTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAATATCAATCTTa                                                                              <  2:344957/149‑1 (MQ=255)
                                                                       aTTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTa                                                                              >  6:261367/1‑149 (MQ=255)
                                                                       aTTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTa                                                                              <  1:381001/149‑1 (MQ=255)
                                                                           gTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAGTTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTa                                                                          >  3:44893/1‑149 (MQ=255)
                                                                              aTGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACt                                                                        <  1:381218/148‑1 (MQ=255)
                                                                                  gAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGAcc                                                                    >  5:147477/1‑148 (MQ=255)
                                                                                      gTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAACTAAATTCTATCAAGGAAGATCAATCTTATCTACTGACCaaaaa                                                               <  6:97654/149‑1 (MQ=255)
                                                                                          tAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGcc                                                           <  3:237001/149‑1 (MQ=255)
                                                                                           aGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCAACAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAGGCCt                                                          <  7:257764/148‑1 (MQ=255)
                                                                                            ggTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCt                                                          <  7:171980/148‑1 (MQ=255)
                                                                                             gTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGa                                                        <  3:141777/149‑1 (MQ=255)
                                                                                              ttCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGa                                                        >  8:309061/1‑148 (MQ=255)
                                                                                              ttCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGa                                                        >  1:84057/1‑148 (MQ=255)
                                                                                               tCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCaaaaa                                                               <  2:381114/140‑1 (MQ=255)
                                                                                               tCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCaaaaa                                                               >  1:381114/1‑140 (MQ=255)
                                                                                                      cTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTc                                               >  1:66842/1‑149 (MQ=255)
                                                                                                      cTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATATTATCATTCTCACACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTATATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTc                                               >  3:273417/1‑149 (MQ=255)
                                                                                                       tattatCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCg                                              >  8:38484/1‑149 (MQ=255)
                                                                                                         ttatCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGaa                                                                                         >  4:179953/1‑104 (MQ=255)
                                                                                                         ttatCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGaa                                                                                         <  3:179953/104‑1 (MQ=255)
                                                                                                            gcGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCa                                          <  1:272948/147‑1 (MQ=255)
                                                                                                              ggCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACta                                       >  2:309249/1‑149 (MQ=255)
                                                                                                              ggCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACt                                        <  2:333187/148‑1 (MQ=255)
                                                                                                              ggCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATATTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACt                                        <  6:308077/148‑1 (MQ=255)
                                                                                                               gCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAg                                                     <  2:318778/134‑1 (MQ=255)
                                                                                                               gCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAg                                                     >  1:318778/1‑134 (MQ=255)
                                                                                                               gCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACta                                       >  7:79294/1‑148 (MQ=255)
                                                                                                                 aCCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACtata                                     >  4:348830/1‑148 (MQ=255)
                                                                                                                   cATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGAAACAGCTGGTGTAAGTAAATTCTATCAACGAAGGTCAATCTTATCTACTGACAAAAAAGGCCTGATAGGGCTTCGATCACTATACa                                   >  8:56983/1‑148 (MQ=255)
                                                                                                                    aTTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTTTCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATc                                 >  5:156472/1‑149 (MQ=255)
                                                                                                                        aaaTCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCtt                              <  5:261367/148‑1 (MQ=255)
                                                                                                                              aaTTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTg                         >  6:105411/1‑147 (MQ=255)
                                                                                                                              aaTTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCa                       <  7:38484/149‑1 (MQ=255)
                                                                                                                                   ttACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGttt                  <  6:187837/149‑1 (MQ=255)
                                                                                                                                   ttACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGtt                   <  5:105411/148‑1 (MQ=255)
                                                                                                                                      cGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAg                <  6:18255/148‑1 (MQ=255)
                                                                                                                                      cGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAg                <  2:120791/148‑1 (MQ=255)
                                                                                                                                           gATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTac          <  2:84057/149‑1 (MQ=255)
                                                                                                                                              cTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGt               >  7:356714/1‑141 (MQ=255)
                                                                                                                                              cTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGt               <  8:356714/141‑1 (MQ=255)
                                                                                                                                               ttATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGACTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTAcacca      >  3:259096/1‑149 (MQ=255)
                                                                                                                                                    aTTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGCTCAATCTTATCTACTGACAAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCATAGGCTGCAGGTTTAGTTGTACACCACTcc  >  2:327323/1‑148 (MQ=255)
                                                                                                                                                    aTTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTcc  >  4:161229/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCC  >  NC_000913/262830‑263125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: