Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	759,249	C→T	R182C (CGC→TGC)	sucA →	2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	759,249	0	C	T	100.0%	58.0 / NA	18	R182C (CGC→TGC)	sucA	2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/9);  total (9/9)

CTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTC  >  NC_000913/759107‑759386                                                                                                                                               |                                                                                                                                          cTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGa                                                                                                                                     <  2:184313/149‑1 (MQ=255)    ccAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATc                                                                                                                                   <  8:157888/148‑1 (MQ=255)          gaCCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGc                                                                                                                            <  4:95493/149‑1 (MQ=255)                   cGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGt                                                                                                                   >  1:3703/1‑149 (MQ=255)                                                 cATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGc                                                                                            >  2:1138/1‑142 (MQ=255)                                                 cATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGc                                                                                            <  1:1138/142‑1 (MQ=255)                                                     aaaCTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGaaaa                                                                                  <  2:3703/148‑1 (MQ=255)                                                                                        cTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTc                                              >  5:129605/1‑149 (MQ=255)                                                                                             tcGGCCCCAATCGGGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGaa                                                  >  7:54457/2‑140 (MQ=255)                                                                                                        ggTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCg                               <  6:129605/148‑1 (MQ=255)                                                                                                              gAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGaaa                                                                                   <  2:15399/90‑1 (MQ=255)                                                                                                              gAGTATATGCACATTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGaaa                                                                                   >  1:15399/1‑90 (MQ=255)                                                                                                                        acaTTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATATCGAAGAGAAAAACCGCGTCTTAAGCGCACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCGGgc              >  3:98595/1‑149 (MQ=255)                                                                                                                          aTTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAag                                                                                        <  5:69242/73‑1 (MQ=255)                                                                                                                          aTTACCAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAag                                                                                        >  6:69242/1‑73 (MQ=255)                                                                                                                               cAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTa                                    >  5:124608/1‑120 (MQ=255)                                                                                                                               cAGCACCGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTa                                    <  6:124608/120‑1 (MQ=255)                                                                                                                                    ccGAAGAAAAATGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTtc  >  3:65502/1‑149 (MQ=255)                                                                                                                                               |                                                                                                                                          CTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTC  >  NC_000913/759107‑759386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑