Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,261,481 G→C A132G (GCT→GGT)  prs ← phosphoribosylpyrophosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,261,4810GC99.2% 459.4 / ‑4.7 127A132G (GCT→GGT) prsphosphoribosylpyrophosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (65/61);  minor base A (1/0);  total (66/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01

Only 100 of 127 total aligned reads displayed.

ATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGATAACAGCGGTGATA  >  NC_000913/1261337‑1261624
                                                                                                                                                |                                                                                                                                               
aTAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTg                                                                                                                                             <  8:61672/149‑1 (MQ=255)
        ggCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCa                                                                                                                                     <  6:52437/149‑1 (MQ=255)
         gcacgcacAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCAc                                                                                                                                    >  7:114831/1‑149 (MQ=255)
          cacgcacAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACt                                                                                                                                   <  6:216486/149‑1 (MQ=255)
          cacgcacAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACt                                                                                                                                   <  8:288667/149‑1 (MQ=255)
             gcacAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGt                                                                                                                                 >  2:295343/1‑148 (MQ=255)
             gaacaACGCCGCCGATCTCCGGAGAAACCACAATTGGGTTATACAGATTCAGCTGCAGCATGTATTCAAGCACGATCGTGCTACCAAATACGTTATCAACCGGAACGTCGAAGAATCCAAGAATCTGTTCACCGAGCAGATCCACTGt                                                                                                                                 >  8:436513/3‑148 (MQ=255)
              cacaACGCCTCCGATGTCCGTATAAACCACAATTGGGTTATCCAGATTCAGCTGAAGCATGTCTTCCAGCAGGATCGGGCTACCAACTACGTTATCAACCGGAACGTCGATGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCa                                                                                                                               <  6:241634/149‑1 (MQ=255)
                caACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAg                                                                                                                              >  1:107003/1‑148 (MQ=255)
                 aaCGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGca                                                                                                                            >  4:500025/1‑149 (MQ=255)
                   cgccgcCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGcaca                                                                                                                          <  1:299488/149‑1 (MQ=255)
                   cgccgcCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGcaca                                                                                                                          >  4:270940/1‑149 (MQ=255)
                     ccgccgATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACg                                                                                                                        >  2:176925/1‑149 (MQ=255)
                      cgccgATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACgg                                                                                                                       >  5:345216/1‑149 (MQ=255)
                        ccgATGTCCGGAGAAACCACAATTGGGTTATCCAGATACAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCACCCGGAACGTCGAAGCAACCCTGAATCTGTTCACCGTGCAGATCCACGGTAAGCACACGGt                                                                                                                      >  6:44791/1‑148 (MQ=255)
                         cgATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTc                                                                                                                     >  2:285684/1‑148 (MQ=255)
                              gccGGAGAAACCACACTTGGGTTATCCAGATTCAGCTGCCGCATGTCTTCCAGCAGGCTCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAacac                                                                                                                <  8:104622/147‑1 (MQ=255)
                                 ggAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGAc                                                                                                            >  3:185005/1‑149 (MQ=255)
                                 ggAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGAc                                                                                                            >  1:152328/1‑149 (MQ=255)
                                       aCCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTg                                                                                                        >  3:480716/1‑147 (MQ=255)
                                       aCCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTg                                                                                                        >  7:200812/1‑147 (MQ=255)
                                       aCCACAATTGGGTTATCCAGATTCAGCTGCAGCATGGCTTCCAGCAGTATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGga                                                                                                      <  5:450947/149‑1 (MQ=255)
                                         cacaATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGgaga                                                                                                    <  1:285684/149‑1 (MQ=255)
                                         cacaATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGga                                                                                                      >  8:133971/1‑147 (MQ=255)
                                         cacaATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGga                                                                                                      >  1:501310/1‑147 (MQ=255)
                                         cacaATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTAGCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGgaga                                                                                                    <  2:485218/149‑1 (MQ=255)
                                          acaATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGagag                                                                                                   <  7:249650/149‑1 (MQ=255)
                                           caATTGGGATATCCAGATTCAGCTGCAGCATGGCATCCAGCAGGATCGGGCTACCAACTACGTTATCACCCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAgg                                                                                                  <  5:291789/149‑1 (MQ=255)
                                            aaTTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAgg                                                                                                  >  1:90810/1‑148 (MQ=255)
                                                gggTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGt                                                                                              <  1:295343/148‑1 (MQ=255)
                                                       ccAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCa                                                                                                                                     <  6:404026/102‑1 (MQ=255)
                                                       ccAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAAc                                                                                       >  3:170230/1‑148 (MQ=255)
                                                        cAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTg                                                                                           <  7:501406/143‑1 (MQ=255)
                                                        cAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTg                                                                                           >  8:501406/1‑143 (MQ=255)
                                                          gATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCCAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCAca                                                                                   >  2:72315/1‑148 (MQ=255)
                                                             tCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTc                                                                                >  4:368982/1‑149 (MQ=255)
                                                                tgtGCAGCATGTCTTCCTGCAGGATCGGGCTACCAAATTCGTTATCAACCGGATGGTCGAAGAAAGCCTGAATCTGTTCACCGTGCAGATCCCCTGTCAGCACACGGTCAACACCGCCGCTGGAGAGGAAGTCTGCAACCACTTTCGCa                                                                             <  2:138022/147‑1 (MQ=255)
                                                                gCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCa                                                                             <  4:170230/149‑1 (MQ=255)
                                                                  tGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAAAACGTTATCAACCGCAACGACGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACAACGACGCTGGAGAGGAATTCTGCAACCACTTTCCCact                                                                           >  4:58823/1‑147 (MQ=255)
                                                                   gcagcaTGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGt                                                                           >  1:479292/1‑148 (MQ=255)
                                                                    cagcaTGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGa                                                                         <  7:436513/149‑1 (MQ=255)
                                                                    cagcaTGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGa                                                                         >  7:94160/1‑149 (MQ=255)
                                                                    cagcaTGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGa                                                                         <  2:120356/149‑1 (MQ=255)
                                                                       caTGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTg                                                                      <  2:501310/149‑1 (MQ=255)
                                                                       caTGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTg                                                                      >  7:158205/1‑149 (MQ=255)
                                                                         tGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTgg                                                                     >  5:354641/1‑148 (MQ=255)
                                                                           tCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTa                                                                   >  5:200981/1‑148 (MQ=255)
                                                                           tCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTa                                                                   <  3:368982/148‑1 (MQ=255)
                                                                           tCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTa                                                                   <  4:185005/148‑1 (MQ=255)
                                                                             ttCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGAtt                                                                       <  3:343800/142‑1 (MQ=255)
                                                                             ttCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGAtt                                                                       >  4:343800/1‑142 (MQ=255)
                                                                             ttCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTacac                                                                >  8:142746/1‑149 (MQ=255)
                                                                             ttCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTacac                                                                >  5:24672/1‑149 (MQ=255)
                                                                                cagcagGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATc                                                                                                                                       >  3:115158/1‑75 (MQ=255)
                                                                                cagcagGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGa                                                              <  5:44791/148‑1 (MQ=255)
                                                                                cagcagGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAg                                                             >  5:393749/1‑149 (MQ=255)
                                                                                 agcagGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGc                                                            <  7:316246/149‑1 (MQ=255)
                                                                                 agcagGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCAACGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGc                                                            >  5:236310/1‑149 (MQ=255)
                                                                                  gcagGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCg                                                           >  6:386002/1‑149 (MQ=255)
                                                                                      gATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAAc                                                       >  7:42882/1‑149 (MQ=255)
                                                                                       aTCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACg                                                      <  3:270940/149‑1 (MQ=255)
                                                                                       aTCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACg                                                      >  1:280937/1‑149 (MQ=255)
                                                                                       aTCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGACTGCAACCACTTTCGCAGTGATTGGGACACGAGCGGAACg                                                      >  8:245066/1‑149 (MQ=255)
                                                                                           ggCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACAGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGAcg                                                  >  1:447957/1‑149 (MQ=255)
                                                                                            gCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGAcgc                                                 >  7:277345/1‑149 (MQ=255)
                                                                                                ccAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTg                                                                      <  1:133010/124‑1 (MQ=255)
                                                                                                ccAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTg                                                                      >  2:133010/1‑124 (MQ=255)
                                                                                                  aaaTACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGt                                           >  4:222080/1‑149 (MQ=255)
                                                                                                       cGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTgg                                      <  8:277345/149‑1 (MQ=255)
                                                                                                        gTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCGACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGgc                                     >  7:546267/1‑149 (MQ=255)
                                                                                                        gTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGgc                                     >  3:215142/1‑149 (MQ=255)
                                                                                                        gTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGgc                                     >  3:315574/1‑149 (MQ=255)
                                                                                                        gTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGgc                                     >  2:378954/1‑149 (MQ=255)
                                                                                                             cAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCa                                >  6:513363/1‑149 (MQ=255)
                                                                                                               aCCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCa                                >  7:313517/1‑147 (MQ=255)
                                                                                                               aCCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCa                                >  3:507549/1‑147 (MQ=255)
                                                                                                               aCCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATa                              <  2:280937/149‑1 (MQ=255)
                                                                                                                ccGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAg                             <  3:222080/149‑1 (MQ=255)
                                                                                                                      cGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAg                       <  6:393749/149‑1 (MQ=255)
                                                                                                                        tCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTa                     <  5:432530/149‑1 (MQ=255)
                                                                                                                          gaagaaACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAgg                   >  2:366923/1‑149 (MQ=255)
                                                                                                                          gaagaaACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAgg                   <  6:354641/149‑1 (MQ=255)
                                                                                                                          gaagaaACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAgg                   <  8:200812/149‑1 (MQ=255)
                                                                                                                          gaagaaACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAgg                   <  8:313517/149‑1 (MQ=255)
                                                                                                                          gaagaaACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAgg                   >  8:415420/1‑149 (MQ=255)
                                                                                                                          gaagaaACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAgg                   <  4:315574/149‑1 (MQ=255)
                                                                                                                          gaagaaACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGCAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAgg                   >  4:164068/1‑149 (MQ=255)
                                                                                                                           aagcaACCCTGAATATGATCACCCTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGATAGGACGTCTGCAACCACTTTCGCAGTGATTGGGACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAggg                  <  6:141505/149‑1 (MQ=255)
                                                                                                                           aagaaACCCTGAATCTGTGCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAggg                  <  1:176925/149‑1 (MQ=255)
                                                                                                                            agaaACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAgggg                 <  5:386002/149‑1 (MQ=255)
                                                                                                                             gaaACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGTGa                <  2:152328/149‑1 (MQ=255)
                                                                                                                             gaaACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGa                <  7:142746/149‑1 (MQ=255)
                                                                                                                                  ccTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGATAACa           >  7:487799/1‑149 (MQ=255)
                                                                                                                                    tGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAg                    >  2:381945/1‑138 (MQ=255)
                                                                                                                                    tGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAg                    <  1:381945/138‑1 (MQ=255)
                                                                                                                                       aTCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGATAACAGCGGt      <  6:200981/149‑1 (MQ=255)
                                                                                                                                           gTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACAAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGATAACAGCGGTGATa  >  3:60681/1‑149 (MQ=255)
                                                                                                                                            ttCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGGAGTGATTGGTACACGAGCGGAACGGAGTCGACGGTCCTGGCGCGCATTGCCAAAGTAGGGGATAACAGCGGTg     <  1:72315/145‑1 (MQ=255)
                                                                                                                                            ttCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGATAACAGCGGTGATa  <  4:507549/148‑1 (MQ=255)
                                                                                                                                            ttCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACACGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGATAACAGCGGTGa    <  2:107003/146‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
ATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGATAACAGCGGTGATA  >  NC_000913/1261337‑1261624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑