Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,227,560 T→C 6.1% intergenic (+49/‑171) metH → / → yjbB homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent/putative Na+/Pi‑cotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,227,5600TC 6.1% 303.7 / 7.0 100intergenic (+49/‑171)metH/yjbBhomocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent/putative Na+/Pi‑cotransporter
Reads supporting (aligned to +/- strand):  ref base T (48/45);  new base C (3/3);  total (52/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATAT  >  NC_000913/4227414‑4227707
                                                                                                                                                  |                                                                                                                                                    
aGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGCATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGCGTGc                                                                                                                                                    >  2:370814/1‑149 (MQ=255)
  cgcgATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGccc                                                                                                                                                  <  1:319308/149‑1 (MQ=255)
   gcgATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCt                                                                                                                                                 >  8:48185/1‑149 (MQ=255)
     gATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGTGCGCTCAATGAGTGCCCTGt                                                                                                                                               <  5:23239/149‑1 (MQ=255)
         aGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGtctc                                                                                                                                            <  3:153604/148‑1 (MQ=255)
         aGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCACTCATTGAGCGCCCTGtttg                                                                                                                                            <  1:223724/148‑4 (MQ=255)
          ggTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCtt                                                                                                                                          <  2:75649/149‑1 (MQ=255)
           gTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCtt                                                                                                                                          >  4:63540/1‑148 (MQ=255)
            ttGTAGATTATGCGCGCAGTTAAGGTATGAGCGTTACCGAAGTTGAGCGGTGGCTGGCAGCGTATCTGGGGTTTGACGCCGACTGATTCAATAAGCTGTAACTTTTCCTTTCATCTAACAGGTCGCTCAATGAGTGCCCTGTCTCTtta                                                                                                                                        <  1:137081/149‑1 (MQ=255)
             tGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTGTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTttat                                                                                                                                       >  6:42771/1‑149 (MQ=255)
                aGATTATGCCCGCCGTAATGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGAAACGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTaa                                                                                                                                    <  1:118954/149‑1 (MQ=255)
                aGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTaa                                                                                                                                    <  7:196021/149‑1 (MQ=255)
                 gATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAat                                                                                                                                   <  2:203108/149‑1 (MQ=255)
                   ttATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAatat                                                                                                                                 <  4:72586/149‑1 (MQ=255)
                      tGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGAAGAGGACTGATTCACAAATCTCTCACTTTTCCTTACACCAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGa                                                                                                                               <  1:265502/148‑1 (MQ=255)
                      agCCCGCCGTAAAGGTATGAGCGTTACCGAAGTGGAGCGCTGGCTGGCACCGAATCTGGGGGATGACGCGGACTGATTCACAAATCCGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGaa                                                                                                                              <  6:297662/148‑1 (MQ=255)
                       gCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGAATGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGaaa                                                                                                                             >  6:120214/1‑149 (MQ=255)
                        cccGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGaaa                                                                                                                             <  8:66223/148‑1 (MQ=255)
                         ccgccgTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAaca                                                                                                                           >  5:306658/1‑149 (MQ=255)
                         ccgccgTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAaca                                                                                                                           >  4:165748/1‑149 (MQ=255)
                          cgccgTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCTCCGACTCTGGGGTATGACCCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATCTGAAacac                                                                                                                          <  8:389173/149‑1 (MQ=255)
                           gccgTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACt                                                                                                                         <  2:212410/149‑1 (MQ=255)
                             cgTAAAGGTATGAGCGTTACCGAAGTTGCGCGCTGGCTGCCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACCAACAGGGCGCTCAATGAGTGACCTGTCTCTTTATTAATATGAAACACTta                                                                                                                       <  2:118995/149‑1 (MQ=255)
                             cgTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTta                                                                                                                       >  3:245197/1‑149 (MQ=255)
                               tAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCGGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTTTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTtata                                                                                                                     <  5:209623/149‑1 (MQ=255)
                                 aaGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATAc                                                                                                                    <  4:245197/148‑1 (MQ=255)
                                   ggTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTgg                                                                                                                 <  8:73832/149‑1 (MQ=255)
                                     taggAGCGTTACCGAAGGTGAGCGCTGGCTGGCACCGAAGCTGGGGTATGACGCGGACTGATTCACAAATCTGTAACTTTTCCTTACAACAAACAGCGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGaa                                                                                                               <  8:366564/146‑1 (MQ=255)
                                       tGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGaaa                                                                                                              >  8:44836/1‑148 (MQ=255)
                                       tGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAAc                                                                                                             <  3:156785/149‑1 (MQ=255)
                                            gTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCGTACAACAAACAGGGCGCTCAATGAGTGCCCGGTCTCTTTATTAATATGAAACACTGATACTGGAAACAGGCt                                                                                                        >  6:219115/1‑149 (MQ=255)
                                                ccGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGaa                                                                                                   <  8:47834/149‑1 (MQ=255)
                                                ccGAAGTTGAGCGCGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGaa                                                                                                   >  1:222830/1‑148 (MQ=255)
                                                 cGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGaa                                                                                                   >  1:277283/1‑148 (MQ=255)
                                                 cGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGaa                                                                                                   >  7:288819/1‑148 (MQ=255)
                                                 cGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGAGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGaa                                                                                                   >  2:96455/1‑148 (MQ=255)
                                                              tggctggcACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCgg                                                                                      >  7:238356/1‑148 (MQ=255)
                                                                gctggcACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGaa                                                                                    >  8:351606/1‑148 (MQ=255)
                                                                gctggcACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGa                                                                                     <  7:395349/147‑1 (MQ=255)
                                                                  tggcACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGACACAGGCTGGAATAATCTTAGCCGGaaaaa                                                                                 >  1:274462/1‑149 (MQ=255)
                                                                     cACCGAATCTTGGGTATGACGCGGACTGATTCACAAATCTGGCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACATATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGa                                                                              <  2:224537/149‑1 (MQ=255)
                                                                      aCCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCACTCATTGAGCGcc                                                                                                                                                   >  8:129049/1‑80 (MQ=255)
                                                                      aCCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCACTCATTGAGCGcc                                                                                                                                                   <  7:129049/80‑1 (MQ=255)
                                                                       ccGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGTAATAATCTTAGCCGGAAAAACGAAt                                                                            <  4:263885/149‑1 (MQ=255)
                                                                        cGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATg                                                                           <  7:48185/149‑1 (MQ=255)
                                                                         gAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGt                                                                          >  5:328166/1‑149 (MQ=255)
                                                                          aaTCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTc                                                                         <  6:306658/149‑1 (MQ=255)
                                                                              tGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCACTCATTGAGCGCCCt                                                                                                                                                 >  1:229735/1‑74 (MQ=38)
                                                                              tGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCACTCATTGAGCGCCCt                                                                                                                                                 <  2:229735/74‑1 (MQ=38)
                                                                                  gTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGca                                                                 >  6:355637/1‑149 (MQ=255)
                                                                                     tGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGcaca                                                               <  8:43028/148‑1 (MQ=255)
                                                                                     tGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGcaca                                                               <  1:271561/148‑1 (MQ=255)
                                                                                     tGACGCGGAATGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGcaca                                                               <  5:120214/148‑1 (MQ=255)
                                                                                              cTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGc                                                     <  8:238356/149‑1 (MQ=255)
                                                                                                 aTTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGGAAAGATGCCa                                                   <  7:54675/148‑1 (MQ=255)
                                                                                                  ttCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGt                                                 <  8:276190/149‑1 (MQ=255)
                                                                                                    cacaAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGt                                                 >  2:279790/1‑147 (MQ=255)
                                                                                                    cacaAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGt                                                 >  3:318529/1‑147 (MQ=255)
                                                                                                     acaAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAg                                                                    >  5:48750/1‑127 (MQ=255)
                                                                                                     acaAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAg                                                                    <  6:48750/127‑1 (MQ=255)
                                                                                                     acaAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAg                                              >  2:153649/1‑149 (MQ=255)
                                                                                                     acaAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTAGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAg                                              >  1:320883/1‑149 (MQ=255)
                                                                                                            tGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGATTAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGttt                                       >  1:130417/1‑149 (MQ=255)
                                                                                                             gTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTc                                      >  7:73943/1‑149 (MQ=255)
                                                                                                              tCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTcc                                     >  6:156567/1‑149 (MQ=255)
                                                                                                              tCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTcc                                     >  4:272029/1‑149 (MQ=255)
                                                                                                              tCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTcc                                     >  7:11318/1‑149 (MQ=255)
                                                                                                                aCTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCg                                    <  8:73943/148‑1 (MQ=255)
                                                                                                                  ttttCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTc                                 >  2:294672/1‑149 (MQ=255)
                                                                                                                  ttttCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTc                                 >  6:103184/1‑149 (MQ=255)
                                                                                                                      ccTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCa                                               >  1:344875/1‑131 (MQ=255)
                                                                                                                      ccTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCa                                               <  2:344875/131‑1 (MQ=255)
                                                                                                                       cTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCttt                             <  5:42771/148‑1 (MQ=255)
                                                                                                                       cTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTg                            <  3:63540/149‑1 (MQ=255)
                                                                                                                       cTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTg                            >  1:57942/1‑149 (MQ=255)
                                                                                                                        ttACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTgg                           >  7:303673/1‑149 (MQ=255)
                                                                                                                        ttACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTgg                           >  3:248674/1‑149 (MQ=255)
                                                                                                                         tACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGATAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTATCCGGTCCTTTGGt                          >  7:336085/1‑149 (MQ=255)
                                                                                                                          acaacaAACAGGGCGATCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACATATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGTTGCCAGTCAGCGTGTTTCCGGTCCTTTGGt                          <  4:46045/148‑1 (MQ=255)
                                                                                                                           caacaaACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCa                        >  6:252782/1‑149 (MQ=255)
                                                                                                                            aacaaacaGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAaca                                                                                                                           >  3:171/1‑50 (MQ=255)
                                                                                                                            aacaaacaGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAaca                                                                                                                           <  4:171/50‑1 (MQ=255)
                                                                                                                            aacaaacaGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCAt                       >  6:7858/1‑149 (MQ=255)
                                                                                                                            aacaaacaGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCAt                       >  3:230159/1‑149 (MQ=255)
                                                                                                                            aacaaacaGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCAt                       >  1:131146/1‑149 (MQ=255)
                                                                                                                             acaaacaGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCAtt                      >  6:341736/1‑149 (MQ=255)
                                                                                                                              caaacaGGGCGCTCAATGAGTGCCCTGTCTCTTTTTAAATTTAAAACCTTATTATGGAAACAGGGAGGAAATATATTTACCCGAAAAAACTAATTAAAAAAGCAAATGAAAAATTGCAGGAAACGTTGTTCCGGTTCGTTGGTGATTc                     >  8:130014/1‑148 (MQ=255)
                                                                                                                              caaacaGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTc                     <  1:279790/149‑1 (MQ=255)
                                                                                                                               aaacaGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTc                     >  5:28613/1‑148 (MQ=255)
                                                                                                                                gacaGGGCACTCATTGAGCGCCCTGTCTATTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGACGTTTGTTCATTaaa                   >  5:16798/2‑149 (MQ=255)
                                                                                                                                 acaGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAAt                  <  7:351606/149‑1 (MQ=255)
                                                                                                                                   aGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTg                >  1:20048/1‑149 (MQ=255)
                                                                                                                                     ggCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCt              <  6:230151/149‑1 (MQ=255)
                                                                                                                                      gcgcTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCtt             <  6:328166/149‑1 (MQ=255)
                                                                                                                                      gcgcTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCtt             >  1:208402/1‑149 (MQ=255)
                                                                                                                                          tCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCTTGTTTCCTGTCCTTTGGTCATTCAATTGCTTCat          >  6:376733/1‑147 (MQ=255)
                                                                                                                                            aaTGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGc               <  8:179439/141‑1 (MQ=255)
                                                                                                                                            aaTGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGc               >  7:179439/1‑141 (MQ=255)
                                                                                                                                                 gTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACatat  <  8:2466/149‑1 (MQ=255)
                                                                                                                                                 gTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCAAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCTATTGCTTCAGGAACata   >  8:139490/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
AGCGCGATCAGGTTGAAGATTATGCCCGCCGTAAAGGTATGAGCGTTACCGAAGTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATAT  >  NC_000913/4227414‑4227707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: