Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	753,309	G→T	5.4%	A387E (GCA→GAA)	gltA ←	citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	753,309	0	G	T	5.4%	272.3 / 3.6	84	A387E (GCA→GAA)	gltA	citrate synthase
Reads supporting (aligned to +/- strand):  ref base G (34/44);  new base T (2/3);  total (36/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01

TATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGCGCGATGTTTTCCAGCTCCATAGCCACTTC  >  NC_000913/753161‑753457                                                                                                                                                     |                                                                                                                                                     tatTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTg                                                                                                                                                      >  5:367451/1‑149 (MQ=255)        aaCTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGc                                                                                                                                               <  6:376269/149‑1 (MQ=255)              gCAATCAACCATTCACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGaaaa                                                                                                                                          <  5:273638/148‑1 (MQ=255)              gCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGaaaa                                                                                                                                          <  5:115855/148‑1 (MQ=255)              gCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAAt                                                                                                                                         <  8:67242/149‑1 (MQ=255)                  tCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACg                                                                                                                                     <  4:173878/149‑1 (MQ=255)                  tCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTAATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACg                                                                                                                                     >  1:57320/1‑149 (MQ=255)                    aaCCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACgg                                                                                                                                    <  5:212182/148‑1 (MQ=255)                      ccATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTg                                                                                                                                  >  4:292213/1‑148 (MQ=255)                        aTTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAAc                                                                                                                               <  2:354785/149‑1 (MQ=255)                            aCGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCGAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATgg                                                                                                                           <  8:316882/149‑1 (MQ=255)                            aCGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATACTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATg                                                                                                                            >  4:321591/1‑148 (MQ=255)                              gCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATgg                                                                                                                           >  4:130532/1‑147 (MQ=255)                              gCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGaa                                                                                                                         <  4:100740/149‑1 (MQ=255)                                ttGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTTATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGa                                                                                                                       <  2:57320/149‑1 (MQ=255)                                ttGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACAt                                                                                                                             <  5:269193/143‑1 (MQ=255)                                ttGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACAt                                                                                                                             >  6:269193/1‑143 (MQ=255)                                  tatatCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTACCATTGCGAAAATGACGGTGAACATGGAAGACg                                                                                                                     <  1:92347/148‑1 (MQ=255)                                    tatCGCTTTTAAAGTCGTGTTTTTCATTTCCTGTATACAGCTGACGCGGACGGGGAAAATACATACCGTCACACTGAATTTAGCTACAGTGGGCGATGCCGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGCCgg                                                                                                                    >  6:15604/1‑148 (MQ=255)                                    tatCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGa                                                                                                                   <  7:279074/149‑1 (MQ=255)                                      tCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGCCGGaa                                                                                                                  >  3:104637/1‑148 (MQ=255)                                      tCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCAATTGCGAAAATGACGGTGAACATGGAAGACGGaa                                                                                                                  >  5:202704/1‑148 (MQ=255)                                         cTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATAcc                                                                                                              >  6:352116/1‑149 (MQ=255)                                             tAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCAt                                                                                                           <  3:65368/148‑1 (MQ=255)                                               aaGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCg                                                                                                         <  1:341396/148‑1 (MQ=255)                                                  tCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGGGATGCAGCGAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCttt                                                                                                     <  2:368552/149‑1 (MQ=255)                                                    gcgTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCa                                                                                                   <  5:124949/149‑1 (MQ=255)                                                    gcgTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCa                                                                                                   <  5:352116/149‑1 (MQ=255)                                                     cgTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAg                                                                                                  <  7:232801/149‑1 (MQ=255)                                                         tttCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGgat                                                                                               >  8:11665/1‑148 (MQ=255)                                                         tttAATCTCCTGTTTACAGCTGACGCGGACGGGCAATATTCATACCGTCACTTTGCATTTCGCTCCAGTGGGCTATCCAGCCTACGGTACGTGCCATTGCGAAAATGACGGTGAACATTGAAGACGGAATACCCATCTCTTTCAGgat                                                                                               <  4:104637/148‑1 (MQ=255)                                                          ttCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGgatg                                                                                              <  8:191970/148‑1 (MQ=255)                                                              tatCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATa                                                                                           >  3:316444/1‑147 (MQ=255)                                                               atCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAAGGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGGTTTCAGGATGATAc                                                                                          >  4:287963/1‑147 (MQ=255)                                                               atCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATAc                                                                                          >  1:259695/1‑147 (MQ=255)                                                               atCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATAc                                                                                          >  3:276946/1‑147 (MQ=255)                                                                tCCTTTATACAGCTGACTCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCTGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCa                                                                                        <  6:315120/148‑1 (MQ=255)                                                                tCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCa                                                                                        <  1:131640/148‑1 (MQ=255)                                                                    gTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGa                                                                                                                       <  7:18066/113‑1 (MQ=255)                                                                    gTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGa                                                                                                                       >  8:18066/1‑113 (MQ=255)                                                                     tataCAGCTGACGCGGAAGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTa                                                                                   <  6:186238/148‑1 (MQ=255)                                                                     gataCCGCTGACGCGTACGGTCAATCTTCATACCGTCACTGGTCCTTTCGCTCCAGGGGGCGATCCAGCCATCGGTACGGTCCATTGCGAAAATGACGGTGAACATGGAAGACGTAATACCCATCGCTGTGAGGATGATACCAGAGTa                                                                                   <  6:260232/147‑1 (MQ=255)                                                                       taCAGCTGACGCGGATGGGCAATCTTCATACCTGCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGCAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGaa                                                                                <  4:124776/149‑1 (MQ=255)                                                                          aGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGTCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATc                                                                             >  8:294180/1‑149 (MQ=255)                                                                          aGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATc                                                                             >  5:19385/1‑149 (MQ=255)                                                                               aCGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGAc                                                                          <  3:292213/147‑1 (MQ=255)                                                                                 gcgGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTc                                                                      >  2:73021/1‑149 (MQ=255)                                                                                 gcgGACGGGCAATCTTCATACAGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTc                                                                      >  1:211/1‑149 (MQ=255)                                                                                   ggACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCgg                                                                    <  4:57399/149‑1 (MQ=255)                                                                                      cGGGCAATGTTCATACCGTCTCTGTGCATTTCGCTCCTGTGGGCGATCGAGCAAACGGTACGTGCCATTGCGATAATGACGGTGAACATGGTTGACGGATTACCCATCGCTTTCAGGATGATTCCAGAGTAGAAATCGACGTTCGGGTa                                                                 <  4:298963/149‑1 (MQ=255)                                                                                       gggCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTCCGGGTa                                                                 >  3:344223/1‑148 (MQ=255)                                                                                         gCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAg                                                              >  7:229765/1‑149 (MQ=255)                                                                                                 cATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTttc                                                          <  6:19385/145‑1 (MQ=255)                                                                                                   tACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGTCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCg                                                    <  7:294180/149‑1 (MQ=255)                                                                                                      cGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTTCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGAt                                                  >  3:325845/1‑148 (MQ=255)                                                                                                      cGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTTCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGAt                                                  >  3:207127/1‑148 (MQ=255)                                                                                                      cGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATa                                                 <  3:321591/149‑1 (MQ=255)                                                                                                       gTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATaa                                                <  7:53661/149‑1 (MQ=255)                                                                                                        tCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATaaa                                               <  4:276946/149‑1 (MQ=255)                                                                                                          aCTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAg                                              >  4:278735/1‑148 (MQ=255)                                                                                                            tgtgCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGCCGGAATACCCCTCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTAc                                           >  1:45793/1‑149 (MQ=255)                                                                                                            tgtgCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTAc                                           >  8:149107/1‑149 (MQ=255)                                                                                                             gtgCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACg                                          >  2:32691/1‑149 (MQ=255)                                                                                                              tgCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTAc                                           <  2:3294/147‑1 (MQ=255)                                                                                                              tgCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGACACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTAc                                           <  5:377709/147‑1 (MQ=255)                                                                                                              tgCATTTCGCTCCAGTGGGCGATCCAGCCAACGGGACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACgg                                         <  4:271639/149‑1 (MQ=255)                                                                                                                cATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTTCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGt                                       <  4:325845/149‑1 (MQ=255)                                                                                                                cATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTTCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGt                                       <  4:207127/149‑1 (MQ=255)                                                                                                                  tttCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCg                                     >  2:97471/1‑149 (MQ=255)                                                                                                                     cGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGtt                                   <  1:97471/148‑1 (MQ=255)                                                                                                                     cGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGtt                                   <  6:202704/148‑1 (MQ=255)                                                                                                                      gCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGtt                                   >  2:357248/1‑147 (MQ=255)                                                                                                                      gCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCa                                 <  1:357248/149‑1 (MQ=255)                                                                                                                          cAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTa                                                                                   <  8:394126/95‑1 (MQ=255)                                                                                                                          cAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTa                                                                                   >  7:394126/1‑95 (MQ=255)                                                                                                                          cAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGTGCGttacccgc                             >  6:131501/1‑143 (MQ=255)                                                                                                                          cAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAgcgc                             >  1:354605/1‑149 (MQ=255)                                                                                                                            gTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGCGCGa                           <  2:211/149‑1 (MQ=255)                                                                                                                             tGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGATCATGGAAGACGGAATACCCATCGCTTTCAGGATGATTCCAGAGTAGATATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGCGCGAt                          <  5:15604/149‑1 (MQ=255)                                                                                                                             tGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGCTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCAATAAAGTACGTGTCGTTCAGCGCGAt                          >  8:371957/1‑149 (MQ=255)                                                                                                                               ggCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGCGCGATGt                        >  3:205135/1‑149 (MQ=255)                                                                                                                                 cGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGCGCGATGttt                      <  8:149881/149‑1 (MQ=255)                                                                                                                                   aTCCAGCCAACGGTACGTGCCATTGCGAAACTGACGGTGATCATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGTGTACCGTTTCTTGTCGATAAAGTACGGGTCGTTAAGCGCTATGTTTTc                    >  8:160824/1‑149 (MQ=255)                                                                                                                                          cAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGCGCGATGTTTTCCAGCTcc             <  3:130532/149‑1 (MQ=255)                                                                                                                                                   ttGCCATTTCGAAAATTACGTTGAACATCGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGATTAGAAATCGAATTTCGTGTACACTTTCTTCTCGATAAATTAAGCGTCGTTAAGCTCGATGTTATCCAGATCCATAGCCaat    >  8:171777/2‑147 (MQ=255)                                                                                                                                                     gCCCTTGCGAAAATGACGGTGAACATGGAAGCCGGAAGACCCCTCGCGTTCAGGATGATACCAGAGTAGCAATCGACGGTCGGGTACAGTTGCTTCTCGATAAAGTACGGGTCGTTCAGCGCGATGTTTTCCAGCTCCATAGCCGCTTc  >  4:292872/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                     TATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGCGCGATGTTTTCCAGCTCCATAGCCACTTC  >  NC_000913/753161‑753457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑