Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,781,138 A→C 6.2% intergenic (+121/‑77) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,781,1380AC 6.2% 282.7 / 6.9 104intergenic (+121/‑77)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base A (62/33);  new base C (2/5);  total (64/40)
Fisher's exact test for biased strand distribution p-value = 9.87e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

CCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCT  >  NC_000913/3780992‑3781283
                                                                                                                                                  |                                                                                                                                                 
ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                                                                                                                 <  1:394694/149‑1 (MQ=255)
ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGga                                                                                                                                                   >  2:211305/1‑147 (MQ=255)
 cATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGagag                                                                                                                                                <  2:235910/149‑1 (MQ=255)
  aTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTAACCCTAACCCTCTCCCTCAGGCAGAGGGGAACGTTCGGCGCTGTATGTACACCCACACTCTGAAACGACACCGCACACGTTTTTTCTCCCTCGCCACTCCGGGGAGAgg                                                                                                                                               >  1:202258/1‑149 (MQ=255)
   tGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAggg                                                                                                                                              <  5:21693/149‑1 (MQ=255)
    ggCGAACGGGACGGCGGCATAGTCGTGTGCGCCCCCTCACCCTCACCCTCTCCCTCAGGGAGAGGGGACCGGGCGGCGCTGTCTGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAggga                                                                                                                                             <  5:200563/149‑2 (MQ=255)
        aaaGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACGCTAAACCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCCCTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCACCGGGGAGAGGGCCggg                                                                                                                                         >  6:163295/1‑149 (MQ=255)
        aaaGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTACCCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCggg                                                                                                                                         <  6:3909/149‑1 (MQ=255)
         aaGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCgggg                                                                                                                                        >  2:66813/1‑149 (MQ=255)
              aaTGCGGCATAGTCGTTTGCGCCCCCTCACCCTATCCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                                                                                   <  1:41825/149‑1 (MQ=255)
              aaTGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                                                                                   <  3:179119/149‑1 (MQ=255)
               aTGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAggg                                                                                                                                  <  1:18380/149‑1 (MQ=255)
                      aTAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTATCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAgg                                                                                                                           >  5:129126/1‑149 (MQ=255)
                                gcgcCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCCCTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGc                                                                                                                  <  3:303858/148‑1 (MQ=255)
                                gcgcCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGCAACGACACCGCACTCTTTTTTTATCCCTCGCCCCTCCGGGGAGAGGGCAGGGGTGCGGGGAAAAGGCCGCCCTGCt                                                                                                                 >  4:101207/1‑149 (MQ=255)
                                gcgcCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAgg                                                                                                                                               <  5:380059/119‑1 (MQ=255)
                                gcgcCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAgg                                                                                                                                               >  6:380059/1‑119 (MQ=255)
                                gcgcCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCt                                                                                                                 >  2:337759/1‑149 (MQ=255)
                                gcgcCCCCGCACCCTAACCCACTCCCACAGGGAGAGGGGACCGTTCGGCGCAGTATGTACTCCCTCACGCTGAAACGACACCCCACTCTTTTTTTATACCACGCCCCACCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCCCTGCt                                                                                                                 >  8:149922/1‑149 (MQ=255)
                                  gcCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCa                                                                                                               >  2:261056/1‑149 (MQ=255)
                                   cccccTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCAc                                                                                                              <  1:211305/149‑1 (MQ=255)
                                    ccccTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCAc                                                                                                              >  1:191137/1‑148 (MQ=255)
                                      ccTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCaa                                                                                                           >  3:339284/1‑149 (MQ=255)
                                      ccTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCaa                                                                                                           >  2:123017/1‑149 (MQ=255)
                                      ccTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCaa                                                                                                           >  3:42178/1‑149 (MQ=255)
                                           ctcTAACCCTCCCCCCCCGGGCGCGGGGACCGTGCGGCGCTGTATGTACCCCCTCCCTCTCAAACCCCCCCGCACTCTTTTTTTCCCCCCCGCCCCCCCGGGGCGAGGGCCGGGGGGAGGGGAAAAGGCCGCACTGCTCACCAATTTTc                                                                                                      <  6:96634/147‑1 (MQ=255)
                                              tAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTc                                                                                                      >  5:96634/1‑146 (MQ=255)
                                                    tctcCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt                                                                                             >  1:136357/1‑149 (MQ=255)
                                                      tcCCTGAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt                                                                                             <  1:37842/147‑1 (MQ=255)
                                                          tCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccccc                                                                                       >  6:170535/1‑149 (MQ=255)
                                                            aGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGc                                                                                     >  3:37657/1‑149 (MQ=255)
                                                                  agGGGACCGTTCGGCGCTGTATGTACACCCTCACTCTTAAACGACACCGAACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGTGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAg                                                                               <  5:163295/149‑1 (MQ=255)
                                                                   ggggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgg                                                                              >  2:12943/1‑149 (MQ=255)
                                                                    gggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggg                                                                             >  6:381163/1‑149 (MQ=255)
                                                                      gACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggggg                                                                           <  4:225934/149‑1 (MQ=255)
                                                                       aCCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggggg                                                                           >  6:304897/1‑148 (MQ=255)
                                                                       aCCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTATTTTTCTCCCTCGCCCATCCGGGGAGAGGGCCGGGGTCAGGCGAAAAGGCCGCACTGCTCACCAATATTCTGAAATCCTGCCCCCGCACTAAggggg                                                                           >  5:173571/1‑148 (MQ=255)
                                                                           tgcGGCCCGGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCGCGCCCCTCCGGGGAGAGGGCCGGGGGGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTa                                                                      <  3:131127/147‑1 (MQ=255)
                                                                              ggCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCTTAGTGCGGGGGCAGGAta                                                                    <  6:129126/148‑2 (MQ=255)
                                                                              ggCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGc                                                                   >  6:296405/1‑149 (MQ=255)
                                                                               gcgcTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGc                                                                   >  6:178961/1‑148 (MQ=255)
                                                                                  cTGTATGTACTCCCTCACTCTGAAACGACCCCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaaa                                                               <  4:42178/149‑1 (MQ=255)
                                                                                        gtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCg                                                          >  8:213250/1‑148 (MQ=255)
                                                                                         taCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTa                                                        >  2:290837/1‑149 (MQ=255)
                                                                                         taCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTa                                                        >  8:4172/1‑149 (MQ=255)
                                                                                             cccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGGATGCTAAACATCGTACtt                                                     >  8:143703/1‑148 (MQ=255)
                                                                                              ccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTa                                                   >  3:99694/1‑149 (MQ=255)
                                                                                               cTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc                                                  >  3:195841/1‑149 (MQ=255)
                                                                                                acACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTAACCAATTTTCTGCTATCATGCCCACGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc                                                  >  8:72762/2‑148 (MQ=255)
                                                                                                  aCTCTTAAACGACACCCCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGTGGGCCGGGGTGTGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGa                                                <  4:210373/148‑1 (MQ=255)
                                                                                                  aCTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGa                                                <  1:123017/148‑1 (MQ=255)
                                                                                                   ctctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAAc                                              <  4:339284/149‑1 (MQ=255)
                                                                                                    tctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAGGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAAc                                              >  1:369817/1‑148 (MQ=255)
                                                                                                    tctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCCCCAATGTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTGTACGAAc                                              >  6:73198/1‑148 (MQ=255)
                                                                                                    tctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAAc                                              >  6:241466/1‑148 (MQ=255)
                                                                                                    tctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAAc                                              >  8:35301/1‑148 (MQ=255)
                                                                                                    tctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCACTCCGGGGAGAGGGACTGGGTGAGGGGAAAAGGCCGGACTTGTGACCAATTTTCTGCTATCCTGACCACGCAGTAAGGGGGCAGTATGCTAAACATAGTTCTTTACGAAc                                              >  6:73199/1‑148 (MQ=255)
                                                                                                    tctGAAACGACACCGCACTCTTATTTTCTCACTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCCTACTTTCCGAAc                                              >  4:261511/1‑148 (MQ=255)
                                                                                                     ctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTCTGCTAAACATCGTACTTTACGAAc                                              >  2:30813/1‑146 (MQ=255)
                                                                                                      tGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAAc                                              >  6:31001/1‑146 (MQ=255)
                                                                                                       gAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAg                                           >  7:250095/1‑148 (MQ=255)
                                                                                                        aaccGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGTGTTGAGGGTAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGa                                          <  3:101207/145‑1 (MQ=255)
                                                                                                         aaCGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGaaa                                        >  4:17101/1‑149 (MQ=255)
                                                                                                                 cGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgggg                                                                            >  6:148144/1‑105 (MQ=255)
                                                                                                                 cGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgggg                                                                            <  5:148144/105‑1 (MQ=255)
                                                                                                                        tttttttCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGGAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTg                         >  6:215977/1‑149 (MQ=255)
                                                                                                                        tttttttCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTa                                                   >  7:253275/1‑123 (MQ=255)
                                                                                                                        tttttttCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATAc                           <  4:195841/147‑1 (MQ=255)
                                                                                                                        tttttttCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTg                         >  5:185329/1‑149 (MQ=255)
                                                                                                                        tttttttCGCCCTCCCCCCTCCGGGGTGTGGGCGGGGGTGAGGGTAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTa                                                   <  8:253275/123‑1 (MQ=255)
                                                                                                                         ttttttCTCCCTAGCCACTCGGTGTAGTGTGACTGGGGTTGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCGGCACTTAGGGGGCAGTATGGTATAGATCGTACTTTACGAACCAGAAATTCCGCGAAATACTg                         <  4:320290/148‑1 (MQ=255)
                                                                                                                          tttttCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTgg                        <  5:31001/148‑1 (MQ=255)
                                                                                                                          tttttCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTgg                        <  5:296405/148‑1 (MQ=255)
                                                                                                                           ttttCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGc                       <  1:261056/148‑1 (MQ=255)
                                                                                                                            cccggcccTCTCCCCGGAGGGGCGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTg                         <  5:86304/140‑1 (MQ=255)
                                                                                                                            cccggcccTCTCCCCGGAGGGGCGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTg                         >  6:86304/6‑145 (MQ=255)
                                                                                                                                tcCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGATCCAGAAATTCCGCCAAATACTGGCAAcat                  >  3:34425/1‑148 (MQ=255)
                                                                                                                                tcCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAcat                  >  5:171355/1‑148 (MQ=255)
                                                                                                                                gcccTCTCCCCGGAGGGGCGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTAAGTTACGAACCAGAAATTCCGCCAAATACTGGCAAgatc                 >  1:119327/2‑149 (MQ=255)
                                                                                                                                  ccTCGCCCCTGCGGGGAGTGGGCGGGGGGGTGGGGAAAAGGCCGCACTGCTCTCCTATTTTCTGCTATCTTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAcat                  <  4:37657/146‑1 (MQ=255)
                                                                                                                                  ccTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAcatcat               >  1:239044/1‑149 (MQ=255)
                                                                                                                                  ccTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAcatcat               >  7:239721/1‑149 (MQ=255)
                                                                                                                                  ccTCCCCCCTCGGGGGTGAGGGCCGGGGTGAGGGTAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAcat                  <  3:18394/146‑1 (MQ=255)
                                                                                                                                   cTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACatcatc              >  4:185217/1‑149 (MQ=255)
                                                                                                                                    tCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCaa                     >  6:91205/1‑141 (MQ=255)
                                                                                                                                    tCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCaa                     <  5:91205/141‑1 (MQ=255)
                                                                                                                                    tCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATcc             >  3:84685/1‑149 (MQ=255)
                                                                                                                                     cGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGAACTGCTAACCAATTTTCTGCTATCCTGCCCACGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCACAAATTCCGCCAAATACTGGCAACATCATCCg            >  5:154427/1‑149 (MQ=255)
                                                                                                                                      gCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATcc             <  6:171355/147‑1 (MQ=255)
                                                                                                                                       ccccGCGGGGGCGTGGGCCGGGGTGAGGGGACAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTc          <  7:149922/149‑1 (MQ=255)
                                                                                                                                        cccTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCt         >  8:32931/1‑149 (MQ=255)
                                                                                                                                        cccTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCt         <  5:304897/149‑1 (MQ=255)
                                                                                                                                        cccTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCt         >  5:157622/1‑149 (MQ=255)
                                                                                                                                        cacTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCt         <  3:94034/147‑1 (MQ=255)
                                                                                                                                         ccTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCtt        >  8:64389/1‑149 (MQ=255)
                                                                                                                                          cTCCGGGTAGAGGGCCGGGGTTCGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTTTCCTGCCACCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTTGCAACATCATCCGTCttt       <  3:261511/149‑1 (MQ=255)
                                                                                                                                          cTCCGGGGAGAGGGGCGGGGTGAGGGGAAAAGGCCGCACTGCTCACGAATTTTCTGCTATGCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCttt       <  6:154427/149‑1 (MQ=255)
                                                                                                                                             cGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAAc                                              >  8:55648/1‑107 (MQ=255)
                                                                                                                                                ggAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCt  >  7:308934/1‑148 (MQ=255)
                                                                                                                                                ggAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCt  >  3:59436/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCT  >  NC_000913/3780992‑3781283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: