Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,548,713 C→T 5.3% S204S (AGC→AGT)  yfeW → penicillin binding protein PBP4B; weak DD‑carboxypeptidase activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,548,7130CT 5.3% 234.9 / 2.3 76S204S (AGC→AGT) yfeWpenicillin binding protein PBP4B; weak DD‑carboxypeptidase activity
Reads supporting (aligned to +/- strand):  ref base C (32/39);  new base T (2/2);  total (35/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.76e-01

GCATCACAGCGGCGGTTTCCCGGCAGATCCGCAATACCCGAATAAAGCGGTCGCGGGCGCGTTATATTCCCAGGATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCTATGTTGAAGAGTCGATTTATCGCCCGCTCGGCCTGACACATACGGTATTTAACCCGCTGCTGAAAGGCTTTAAACCG  >  NC_000913/2548566‑2548854
                                                                                                                                                   |                                                                                                                                             
aacgtacaGCGGCGGTTTCCCGGCAGATCCGCAATACCCGAATAAAGCGGTCGCGGGCGCGTTATATTCCCAGGATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCg                                                                                                                                              <  7:77523/144‑1 (MQ=255)
  aTCACAGCGGCGGTTTCCCGGCAGATCCGCAATACCCGAATAAAGCGGTCGCGGGCGCGTTATATTCCCAGGATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGAt                                                                                                                                            >  7:287642/1‑149 (MQ=255)
   tCACAGCGGCGGTTTCCCGGCAGATCCGCAATACCCGAATAAAGCGGTCGCGGGCGCGTTATATTCCCAGGATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATg                                                                                                                                           >  5:18864/1‑149 (MQ=255)
   tCACAGCGGCGGTTTCCCGGCAGATCCGCAATACCCGAATAAAGCGGTCGCGGGCGCGTTATATTCCCAGGATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATg                                                                                                                                           <  4:45173/149‑1 (MQ=255)
   tCACAGCGGCGGTGTCCCGGCAGATCCGCAATACCCGAATAAAGCGGTCGCGGGCGCGGTATATTCCCAGGATAAAGCCCAGACGCTCGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGaac                                                                                                                                           >  7:284062/1‑147 (MQ=255)
    cacaGCGGCGGTTTCCCGGCAGATCCGCAATACCCGAATAAAGCGGTCGCGGGCGCGTTATATTCCCAGGATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGt                                                                                                                                          >  1:288791/1‑149 (MQ=255)
     acaGCGGCGGTTTCCCGGCAGATCCGCAATACCCGAATAAAGCGGTCGCGGGCGCGTTATATTCCCAGGATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGt                                                                                                                                          <  2:209473/148‑1 (MQ=255)
     acaGCGGCGGTTTCCCGGCAGATCCGCAATACCCGAATAAAGCGGTCGCGGGCGCGTTATATTCCCAGGATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATATACAGCGATGTc                                                                                                                                         >  8:388400/1‑149 (MQ=255)
     acaGCGGCGGTTTCCCGGCAGATACGCAATACCCGAATAAAGCGGTCTCGGGCGCGTTATATTCCCAGGATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTc                                                                                                                                         >  8:117357/1‑149 (MQ=255)
        gcggcgGTTTCCCGGCAGATCCGCAATACCCGAATAAAGCGGTCGCGGGCGCGTTATATTCCCAGGATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGAt                                                                                                                                      <  7:237821/149‑1 (MQ=255)
                   ccGGCAGATCCGCAATACCCGAATAAAGCGGTCGCGGGCTCGTTATATTCCCAGGATAACGGCCAGCCGCTGGAGATGATCAAGCGCACGCCGCGGGAATATCAGCCCTGCAGCAAACATATCTACAGCGATGTCGAGTATATgctgct                                                                                                                           >  3:226658/1‑149 (MQ=255)
                   ccGGCAGATCCGCAATACCCGAATAAAGCGGTCGCGGGCGCGTTATATTCCCAGGATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATgctgct                                                                                                                           >  2:47259/1‑149 (MQ=255)
                   ccGGCAGATCCGCAATACCCGAATAAAGCGGTCGCGGGCGCGTTATATTCCCAGGATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATgctgct                                                                                                                           >  1:197359/1‑149 (MQ=255)
                   ccGGCAGATCCGCAATACCCGAATAAAGCGGTCGCGGGCGCGTTATATTCCCAGGATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATgctgct                                                                                                                           >  6:339651/1‑149 (MQ=255)
                        aGATCCGCAATACCCGAATAAAGCGGTCGCGGGCGCGTTATATTCCCAGGATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGAt                                                                                                                      >  8:26474/1‑149 (MQ=255)
                        aGATCCGCAATACCCGAATAAAGCGGTCGCGGGCGCGTTATATTCCCAGGATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGAt                                                                                                                      <  7:33890/149‑1 (MQ=255)
                             cGCAATACCCGAATAAAGCGGTCGCGGGCGCGTTATATTCCCAGGATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTAtc                                                                                                                 <  1:47259/149‑1 (MQ=255)
                               cAATACCCGAATAAAGCGGTCGCGGGCGCGTTATATTCCCAGGATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTAtcgt                                                                                                               <  3:68121/149‑1 (MQ=255)
                               cAATACCCGAATAAAGCGGTCGCGGGCGCGTTATATTCCCAGGATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTAtcgt                                                                                                               <  3:63204/149‑1 (MQ=255)
                                aaTACCCGAATAAAGCGGTCGCGGGCGCGTTATATTCCCAGGATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTAtcgtc                                                                                                              <  4:284197/149‑1 (MQ=255)
                                     ccGAATAAAGCGGTCGCGGGCGCGTTATATTCCCAGGATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTc                                                                                                         >  4:327903/1‑149 (MQ=255)
                                     ccGAATAAAGCGGTCGCGGGCGCGTTATATTCCCAGGATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATACGCTGCTTGGAGTTATCGTCGAGTc                                                                                                         >  7:391960/1‑149 (MQ=255)
                                            aaGCGGTCGCGGGCGCGTTATATTCCCAGGATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCATCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTAc                                                                                                   <  2:197359/148‑1 (MQ=255)
                                                ggTCGCGGGCGCGTTATATTCCCAGGATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGt                                                                                                      <  6:255646/141‑1 (MQ=255)
                                                ggTCGCGGGCGCGTTATATTCCCAGGATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGt                                                                                                      >  5:255646/1‑141 (MQ=255)
                                                  tCGCGGGCGCGTTATATTCCCAGGATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAg                                                                                                           >  3:152261/1‑134 (MQ=255)
                                                  tCGCGGGCGCGTTATATTCCCAGGATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAg                                                                                                           <  4:152261/134‑1 (MQ=255)
                                                      gggCGCGTTATATTCCCAGGATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGc                                                                                        <  2:153632/149‑1 (MQ=255)
                                                        gcgcGTTATATTCCCAGGATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCtt                                                                                      <  5:28594/149‑1 (MQ=255)
                                                           cgTTATATTCCCAGGATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGAc                                                                                   <  7:328278/149‑1 (MQ=255)
                                                            gTTATATTCCCAGGATAAAGGCCAGCCGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGAGTATATGCTGCTTGGATGTATCGTCGAGTCGGTTACCGGTCAGCCGGGTGAcc                                                                                  >  8:350673/1‑149 (MQ=255)
                                                            gTTATATTCCCAGGATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGAcc                                                                                  >  3:105878/1‑149 (MQ=255)
                                                                    cccAGCATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGGCGGTTACCGGTCAGCCGCTTGACCGCTATGt                                                                           <  7:117357/148‑1 (MQ=255)
                                                                     ccAGGATAAAGGCCAGACGCTGGAGATGATCAAGCACACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGTGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCtt                                                                                      >  5:326920/1‑136 (MQ=255)
                                                                     ccAGGATAAAGGCCAGACGCTGGAGATGATCAAGCACACGCAGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGTGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCtt                                                                                      <  6:326920/136‑1 (MQ=255)
                                                                      cAGGATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGATTATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCTATGTTGa                                                                        <  1:123568/149‑1 (MQ=255)
                                                                      cAGGATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCACTCATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCTATGTTg                                                                         >  3:216257/1‑148 (MQ=255)
                                                                      cAGGATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCTATGTTg                                                                         >  3:163094/1‑148 (MQ=255)
                                                                       aGGATAAACGACAGACGCTGGAGATGATCCAGCGCACGCCGCTGCAATATCATCCGGGAATCAAACATATCTCCAGCGATGTAGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTGACCGGTCAGCCGCTTGACCGCTATGTTGaa                                                                       <  2:363383/149‑1 (MQ=255)
                                                                         gATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCTATGTTGaa                                                                       >  7:70905/1‑147 (MQ=255)
                                                                           tAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCTATGTTGAAGAGt                                                                   >  8:272885/1‑149 (MQ=255)
                                                                           acaaGGCCAGACGCTCGAGATGAGCAAGCGCACGCCGCAGGAATATCCGCCCCGCCGCACACATCTCTACAGCGATGTCGAGTCTATGCTGCTTGGCGTTATCGTCTACTCGGCTACCGGGCCGCCTCTTGACCGCTATGTTGAAGAGt                                                                   <  8:284062/147‑1 (MQ=255)
                                                                              aGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGAtt                                                                                                                                     <  4:261764/80‑1 (MQ=255)
                                                                              aGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGAtt                                                                                                                                     >  3:261764/1‑80 (MQ=255)
                                                                              aGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCTATGTTGAAGAGTCGa                                                                <  6:349298/149‑1 (MQ=255)
                                                                              aGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCTATGTTGAAGAGTCGa                                                                <  6:367021/149‑1 (MQ=255)
                                                                                gCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCTATGTTGAAGAGTCGAtt                                                              <  8:164994/149‑1 (MQ=255)
                                                                                  cAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCTATGTTGAAGAGTCGATTTa                                                            >  8:388152/1‑149 (MQ=255)
                                                                                  cAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGAGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCTATGTTGAAGAGTCGATTTa                                                            >  1:161014/1‑149 (MQ=255)
                                                                                        cTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCTATGTTGAAGAGTCGATTTATCGccc                                                      <  7:272885/149‑1 (MQ=255)
                                                                                         tGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCTATGTTGAAGAGTCGATTTATCGCCCg                                                     >  8:381818/1‑149 (MQ=255)
                                                                                           gagaTGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATAATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCAATGTTGAAGAGTCGATTTATCGCCAGCt                                                   >  8:63513/1‑149 (MQ=255)
                                                                                                    aaGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCTATGTTGAAGAGTCGATTTATCGCCCGCTCGGCCTGa                                           >  7:190642/1‑148 (MQ=255)
                                                                                                    aaGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCTATGTTGAAGAGTCGATTTATCGCCCGCTCGGCCTGa                                           <  5:339651/148‑1 (MQ=255)
                                                                                                    aaGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCTATGTTGAAGAGTCGATTTATCGCCCGCTCGGCCTGa                                           <  3:349197/148‑1 (MQ=255)
                                                                                                      gcgcACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGCTACCGGTCAGCCGCTTGACCGCTATGTTGAAGAGTCGATTTAGCGCCCGCTCGGCCTGacac                                        <  2:288791/149‑1 (MQ=255)
                                                                                                        gcACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCTATGTTGAAGAGTCGATTTATCGCCCGCTCGGCCTGACACAt                                      <  7:158409/149‑1 (MQ=255)
                                                                                                          aCGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCTATGTTGAAGAGTCGATTTATCGCCCGCTCGGCCTGACACATAc                                    <  8:70905/149‑1 (MQ=255)
                                                                                                           cgccgcTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCTATGTTGAAGAGTCGATTTATCGCCCGCTCGGCCTGACACATACg                                   <  5:331865/149‑1 (MQ=255)
                                                                                                           cgccgcTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCTATGTTGAAGAGTCGATTTATCGCCCGCTCGGCCTGACACATACg                                   <  3:327903/149‑1 (MQ=255)
                                                                                                                  ggAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCTATGTTGAAGAGTCGATTTATCGCCCGCTCGGCCTGACACATACGGTAttt                             >  3:222258/1‑148 (MQ=255)
                                                                                                                   gAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATAGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCTATGTTGAAGAGTCGATTTATCGCCCGCTCGGCCTGACACATACGGTATTTaa                           <  3:370062/149‑1 (MQ=255)
                                                                                                                    aaTATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCTATGTTGAAGAGTCGATTTATCGCCCGCTCGGCCTGACACATACGGTATTTAAc                          >  5:323066/1‑149 (MQ=255)
                                                                                                                            cccGGCAGCAAACATATCTACAGCGATGTCGATGATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCTATGTTGAAGAGTCGATTTATCGCCCGCTCGGCCTGACACATACGGTATTTAACCCgctgct                  <  8:174086/149‑1 (MQ=255)
                                                                                                                             ccGGCAGCAAACATATCTACAGTGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCTATGTTGAAGAGTCGATTTATCGCCCGCTCGGCCTGa                                           <  6:270218/123‑1 (MQ=255)
                                                                                                                             ccGGCAGCAAACATATCTACAGTGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCTAGGTTGAAGAGTCGATTTATCGCCCGCTCGGCCTGa                                           >  5:270218/1‑123 (MQ=255)
                                                                                                                                  agcaAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCTATGTTGAAGAGTCGATTTATCGCCCGCTCGGCCTGACACATACGGTATTTAACCCGCTGCTGAAAgg            <  7:26474/149‑1 (MQ=255)
                                                                                                                                    caAACATAGCTACAGCGATGTAGATTATATGCTGCTTGGATTTATCGTCGAGGCGGTTACGGGTCAGCCGCTTGAGCGCTATGTTGAAGAGTCGATTTATCGCCCGCTCGGCCTGACACATACGGTATTTAAGCCGCTGCTGAAAGGCt          <  5:241100/149‑1 (MQ=255)
                                                                                                                                     aaaCATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCTATGTTGAAGAGTCGATTTATCGCCCGCTCGGCCTGACACATACGGTATTTAACCCGCTGCTGAAAGGCtt         >  3:229936/1‑149 (MQ=255)
                                                                                                                                     aaaCATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTAAGCCGCTTGACCGCTATGTTGAAGAGGCGATTTATCGCCCGATCGGCCTGAAACATACGGTATTTAACCCGCTGCTGAAATGCt          <  4:226658/148‑1 (MQ=255)
                                                                                                                                       aCATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCTATGTTGAAGAGTCGATTTATCGCCCGCTCGGCCTGACACATACGGTATTTAACCCGCTGCTGAAAGGCttt        >  7:84573/1‑148 (MQ=255)
                                                                                                                                         atatCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCTATGTTGAAGAGTCGATTTATCGCCCGCTCGGCCTGACACATACGGTATTTAACCCGCTGCTGAAAGGCTTTaaa     <  4:229936/149‑1 (MQ=255)
                                                                                                                                            tCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCTATGTTGAAGAGTCGATTTATCGCCCGCTCGGCCTGACACATACGGTATTTAACCCGCTGCTGAAAGGCTTTAAACCg  >  7:114375/1‑149 (MQ=255)
                                                                                                                                             cTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCTATGTTGAAGAGTCGATTTATCGCCCGCTCGGCCTGACACATACGGTATTTAACCCGCTGCTGAAAGGCTTTAAACCg  >  2:302307/1‑148 (MQ=255)
                                                                                                                                                cAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCTATGTTGAAGAGTCGATTTATCGCCCGCTCGGCCTGACACATACGGTATTTAACCCGCTGCTGAAAGGCTTTAAACCg  <  8:287642/145‑1 (MQ=255)
                                                                                                                                                cAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCTATGTTGAAGAGTCGATTTATCGCCCGCTCGGCCTGACACATACGGTATTTAACCCGCTGCTGAAAGGCTTTAAACCg  <  7:381818/145‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
GCATCACAGCGGCGGTTTCCCGGCAGATCCGCAATACCCGAATAAAGCGGTCGCGGGCGCGTTATATTCCCAGGATAAAGGCCAGACGCTGGAGATGATCAAGCGCACGCCGCTGGAATATCAGCCCGGCAGCAAACATATCTACAGCGATGTCGATTATATGCTGCTTGGATTTATCGTCGAGTCGGTTACCGGTCAGCCGCTTGACCGCTATGTTGAAGAGTCGATTTATCGCCCGCTCGGCCTGACACATACGGTATTTAACCCGCTGCTGAAAGGCTTTAAACCG  >  NC_000913/2548566‑2548854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑