Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,685,802 T→A 5.1% N263Y (AAC→TAC)  fumC ← fumarate hydratase (fumarase C),aerobic Class II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,685,8020TA 5.1% 256.2 / 3.5 79N263Y (AAC→TAC) fumCfumarate hydratase (fumarase C),aerobic Class II
Reads supporting (aligned to +/- strand):  ref base T (34/40);  new base A (2/2);  total (36/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

GGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTC  >  NC_000913/1685654‑1685943
                                                                                                                                                    |                                                                                                                                             
gggATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGtt                                                                                                                                               <  5:173452/149‑1 (MQ=255)
 ggATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTc                                                                                                                                              <  6:359357/149‑1 (MQ=255)
  gATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCg                                                                                                                                             <  3:263349/149‑1 (MQ=255)
    ttGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGgc                                                                                                                                           >  3:11734/1‑149 (MQ=255)
        gaTTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCgg                                                                                                                                       >  8:274063/1‑149 (MQ=255)
              aCCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACa                                                                                                                                  >  7:57888/1‑148 (MQ=255)
                cAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCCACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAAc                                                                                                                               >  4:129505/1‑149 (MQ=255)
                cAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAAc                                                                                                                               >  7:383712/1‑149 (MQ=255)
                  aTTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCCACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACgg                                                                                                                             <  3:129505/149‑1 (MQ=255)
                     ccGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTg                                                                                                                           <  5:324783/148‑1 (MQ=255)
                      cGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGca                                                                                                                         >  5:285485/1‑149 (MQ=255)
                      cGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGca                                                                                                                         >  1:93166/1‑149 (MQ=255)
                      cGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGca                                                                                                                         >  2:394010/1‑149 (MQ=255)
                       gCAGCGCGGGCCAGAGGCCCGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGca                                                                                                                         >  5:137533/1‑148 (MQ=255)
                       gCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGcac                                                                                                                        <  5:300218/149‑1 (MQ=255)
                             cgGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGGCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAACGGTGCACAGGTAAt                                                                                                                 <  4:288393/149‑1 (MQ=255)
                                 ccAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGAc                                                                                                              <  2:259370/149‑1 (MQ=255)
                                   agagGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTg                                                                                                            >  4:8348/1‑149 (MQ=255)
                                    gagGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGc                                                                                                           <  6:327747/149‑1 (MQ=255)
                                    gagGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGc                                                                                                           <  6:121542/149‑1 (MQ=255)
                                        ccagccagCGGACATCATTGGCGATTTTCATCAGTGCCGCCGCCAACCCTTTCAACGCGCCGTGCGGCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGt                                                                                                       <  3:202055/149‑1 (MQ=255)
                                        ccagccagCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGt                                                                                                       <  2:82255/149‑1 (MQ=255)
                                           gccagcGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCa                                                                                                    >  3:302062/1‑149 (MQ=255)
                                                  gACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTa                                                                                             <  5:111760/149‑1 (MQ=255)
                                                     atcatTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTacgc                                                                                          <  5:90394/149‑1 (MQ=255)
                                                       catTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACa                                                                                                                                  <  7:302990/107‑1 (MQ=255)
                                                       catTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACa                                                                                                                                  >  8:302990/1‑107 (MQ=255)
                                                         ttGGCGATTTTCATCAGTGCCGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGGGCACAGGTAATGACTGCCAGTTCATCTGCTacgcgccg                                                                                      >  5:304827/1‑149 (MQ=255)
                                                          tGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCa                                                                                                    <  8:283590/134‑1 (MQ=255)
                                                          tGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCa                                                                                                    >  7:283590/1‑134 (MQ=255)
                                                           ggCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTCACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTccgcgacgcg                                                                                    >  6:340680/1‑149 (MQ=255)
                                                           ggCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTacgcgacgc                                                                                     <  8:383712/148‑1 (MQ=255)
                                                            gCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTacgcgacgcg                                                                                    <  6:285485/148‑1 (MQ=255)
                                                            gCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTacgcgacgcg                                                                                    >  7:142026/1‑148 (MQ=255)
                                                             cGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCa                                                                                  <  3:275714/149‑1 (MQ=255)
                                                               aTTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATa                                                                                <  3:122509/149‑1 (MQ=255)
                                                                   tcatcaGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTcc                                                                            >  1:235869/1‑149 (MQ=255)
                                                                      tcaGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGa                                                                         <  2:93166/149‑1 (MQ=255)
                                                                        aGTTACGCAGCGAACCCTTTCTACGCGCGGTGCGCCTTAACCAGTTCATCACTGGTCGCGAGCGCTTCAATTTTGTTCGGCGCGGTAACAAACGGTGCACTGGTAATGTCTGCCAGTTCATCTGCTACTCGAAGCTCATAATCCGGATg                                                                       <  8:233134/149‑1 (MQ=255)
                                                                         gTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATgg                                                                      <  2:116397/149‑1 (MQ=255)
                                                                         gTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATgg                                                                      <  7:343435/149‑1 (MQ=255)
                                                                          tGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCa                                                                                                         >  8:25586/1‑113 (MQ=255)
                                                                          tGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCa                                                                                                         <  7:25586/113‑1 (MQ=255)
                                                                            aCGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATTGGt                                                                    <  2:155523/148‑1 (MQ=255)
                                                                            aCGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTa                                                                   <  8:142026/149‑1 (MQ=255)
                                                                              gCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTCACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTAtt                                                                 >  6:353297/1‑149 (MQ=255)
                                                                              gCAGCCAACCCTTTCAACGCGCCGTGCGCCTCAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACACACGGTGCACAGGTAATGACGGCCAGTTCATCTGCTACGCGACGCGCATACTCCGCATGCGTAtt                                                                 >  5:206181/1‑149 (MQ=255)
                                                                                aGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACATGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTAttca                                                               <  8:40696/149‑3 (MQ=255)
                                                                                   catcccTTTCAAGGCGCCGTGCGCATGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTc                                                            <  6:137533/146‑1 (MQ=255)
                                                                                       ccTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAg                                                         >  1:190841/1‑148 (MQ=255)
                                                                                        cTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTa                                                       <  1:394010/149‑1 (MQ=255)
                                                                                        cTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTa                                                       <  8:57888/149‑1 (MQ=255)
                                                                                        cTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTGCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTa                                                       <  7:274063/149‑1 (MQ=255)
                                                                                         tttCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTa                                                               >  5:9061/1‑140 (MQ=255)
                                                                                         tttCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTa                                                               <  6:9061/140‑1 (MQ=255)
                                                                                             aaCGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCt                                                                                              >  6:232095/1‑105 (MQ=255)
                                                                                             aaCGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCt                                                                                              <  5:232095/105‑1 (MQ=255)
                                                                                                gcgcCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGc                                               >  1:361640/1‑149 (MQ=255)
                                                                                                  gcCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTg                                             <  4:143823/149‑1 (MQ=255)
                                                                                                    cGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTa                                           <  2:165244/149‑1 (MQ=255)
                                                                                                      tGCGCCTGACCCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTAcc                                         >  6:295235/1‑149 (MQ=255)
                                                                                                         gcCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGc                                       >  8:55987/1‑148 (MQ=255)
                                                                                                           cTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCaa                                    <  4:11734/149‑1 (MQ=255)
                                                                                                              aaCCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAaga                                  <  2:235869/148‑1 (MQ=255)
                                                                                                               aCCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGc                                >  8:65372/1‑149 (MQ=255)
                                                                                                               aCCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGc                                <  2:361640/149‑1 (MQ=255)
                                                                                                                   gggCATCACAGGTCGCCAGCGCTTCAAATTTGTACGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTAGGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCaa                                    <  7:255747/141‑1 (MQ=255)
                                                                                                                   gggCATCACAGGTCGCCAGCGCTTCAAATTTGTACGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTAGGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCaa                                    <  7:250018/141‑1 (MQ=255)
                                                                                                                   gggCATCACAGGTCGCCAGCGCTTCAAATTTGTACGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTAGGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCaa                                    >  8:250018/1‑141 (MQ=255)
                                                                                                                   gggCATCACAGGTCGCCAGCGCTTCAAATTTGTACGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTAGGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCaa                                    >  8:255747/1‑141 (MQ=255)
                                                                                                                       aTCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTAtt                                                                 <  1:230257/108‑1 (MQ=255)
                                                                                                                       aTCACAGGTCGCCAGCGCTTCAAATTTGTTCGGAGCGGTAACAAAAGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTAtt                                                                 >  2:230257/1‑108 (MQ=255)
                                                                                                                        tCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGc                       >  5:234535/1‑149 (MQ=255)
                                                                                                                         cacaGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGc                       >  2:154502/1‑148 (MQ=255)
                                                                                                                              gTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGAATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGt                  >  8:286016/1‑148 (MQ=255)
                                                                                                                                     gcgcTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGaggcagg          <  2:190841/149‑1 (MQ=255)
                                                                                                                                       gcTTCAAATTTGTTCGGCGCGGTAACAAACGGTTCACAGGTAATGACTGGCAGTTCATCTGCTACGCGACGCGCATACTCCTGATGGGTATTTTGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCt        <  6:206181/149‑1 (MQ=255)
                                                                                                                                         ttCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCTACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGGGAGGCAGGCTGt      >  2:99359/1‑149 (MQ=255)
                                                                                                                                          tCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTa     >  3:50240/1‑149 (MQ=255)
                                                                                                                                              aTTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTc  >  2:364247/1‑148 (MQ=255)
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GGGATTGAGATTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTC  >  NC_000913/1685654‑1685943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: