Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,261,481 G→C 100% A132G (GCT→GGT)  prs ← phosphoribosylpyrophosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,261,4810GC100.0% 248.4 / ‑6.7 70A132G (GCT→GGT) prsphosphoribosylpyrophosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (31/38);  minor base A (1/0);  total (32/38)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGATAACAGCGGTGATA  >  NC_000913/1261337‑1261624
                                                                                                                                                |                                                                                                                                               
aTAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTg                                                                                                                                             <  6:319277/149‑1 (MQ=255)
 tAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGc                                                                                                                                            <  7:361337/149‑1 (MQ=255)
   gcgcGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGc                                                                                                                                            <  2:53641/147‑1 (MQ=255)
   gcgcGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGc                                                                                                                                            >  1:53641/1‑147 (MQ=255)
     gcgGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGAt                                                                                                                                        <  2:134300/149‑1 (MQ=255)
          cacgcacAACGCCGCCGATGGCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACt                                                                                                                                   <  7:368629/149‑1 (MQ=255)
              cacaACTCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTAAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCa                                                                                                                               <  5:228029/149‑1 (MQ=255)
               acaACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAGCCCTGAATCTGTTCACCGTGCAGATCCACTGTCAg                                                                                                                              >  3:180789/1‑149 (MQ=255)
               acaACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAg                                                                                                                              <  1:32724/149‑1 (MQ=255)
                  aCGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCTAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGcac                                                                                                                           <  6:194/149‑1 (MQ=255)
                        ccgATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTc                                                                                                                     <  8:159386/149‑1 (MQ=255)
                           aTGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCaa                                                                                                                   >  2:44484/1‑148 (MQ=255)
                                     aaaCCACAATTGGGTTATGCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCATCCGGAACGTCGAAGAAACCCTGAATCTGTGCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTg                                                                                                        <  2:150233/149‑1 (MQ=255)
                                       aCCACAATTGGGTTATCAAGATTCAGCTGCAGCATGTCTTACAGCAGTATCTGGCTACCAAATACGTTATCAACCGGAACGTCGAAGCAACCATGAATCTGTTCAAAGTGCAGATCCACTGTCAGAACACGGTCAACACCGCCGCTg                                                                                                        >  8:27725/1‑147 (MQ=255)
                                        ccACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTg                                                                                                        >  6:204194/1‑146 (MQ=255)
                                         cacaATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGga                                                                                                      >  1:14945/1‑147 (MQ=255)
                                         cacaATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGga                                                                                                      >  7:76566/1‑147 (MQ=255)
                                            aaTTGGGTTATCCAGATTCAGCTGCAGAATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTCTCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGa                                                                                                 <  7:288954/149‑1 (MQ=255)
                                             aTTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGaa                                                                                                <  2:33247/149‑1 (MQ=255)
                                                 ggTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCt                                                                                            <  4:148836/149‑1 (MQ=255)
                                                  gTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTg                                                                                           >  3:304397/1‑149 (MQ=255)
                                                   ttATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTg                                                                                           >  1:212248/1‑148 (MQ=255)
                                                   ttATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGc                                                                                          <  6:224764/149‑1 (MQ=255)
                                                       ccAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAAc                                                                                       <  4:180789/148‑1 (MQ=255)
                                                               aGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCg                                                                               >  8:196928/1‑148 (MQ=255)
                                                                gCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCg                                                                               >  1:118036/1‑147 (MQ=255)
                                                                gCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAAGCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCa                                                                             <  8:90704/149‑1 (MQ=255)
                                                                     agcaTGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGAt                                                                        <  5:279613/149‑1 (MQ=255)
                                                                       caTGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTg                                                                      <  1:44484/149‑1 (MQ=255)
                                                                       caTGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTg                                                                      >  3:254636/1‑149 (MQ=255)
                                                                        aTGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTgg                                                                     <  2:14945/149‑1 (MQ=255)
                                                                         tGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCt                                                                                            >  2:28895/1‑125 (MQ=255)
                                                                         tGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCt                                                                                            <  1:28895/125‑1 (MQ=255)
                                                                           tCTTCCAGCAGGATCGGGCTACCAAATACGTTATCACCCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCGGGAGAGGAAGGCTGCAACCACTTTCGCAGTGATTGGTa                                                                   <  2:380908/148‑1 (MQ=255)
                                                                           tCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTa                                                                   <  5:294743/148‑1 (MQ=255)
                                                                             ttCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTacac                                                                >  8:152602/1‑149 (MQ=255)
                                                                               ccAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTacac                                                                <  4:254636/147‑1 (MQ=255)
                                                                                 agcagGATCGGGCTCCCAAATACGATATCCACCGGAACGTCGACGAAACCCTGCATCTGTTCACCGTCCAGATCCACTGTCACCACACGGTCAACACCGACGCTGGAGAGGACGTCTGCCACCACTTTCGCAGTGATTCGTACACGAGc                                                            <  2:330517/149‑1 (MQ=255)
                                                                                 agcagGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGc                                                            <  7:71373/149‑1 (MQ=255)
                                                                                                  aaaTACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGt                                           >  4:338839/1‑149 (MQ=255)
                                                                                                  aaaTACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGt                                           <  7:196928/149‑1 (MQ=255)
                                                                                                    aTACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGACAACACCGACGCTGGAGAGGAAGTCTCCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTcc                                         >  5:30143/1‑149 (MQ=255)
                                                                                                      aCGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGCCGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTg                                       >  2:375642/1‑149 (MQ=255)
                                                                                                        gTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGACAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGgc                                     >  8:72910/1‑149 (MQ=255)
                                                                                                         ttATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGgcg                                    >  6:170279/1‑149 (MQ=255)
                                                                                                         ttATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGCCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGgcg                                    >  1:289819/1‑149 (MQ=255)
                                                                                                          tATCAACCGGAACGTCGAAGAACCCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGgcgc                                   >  5:73353/1‑149 (MQ=255)
                                                                                                            tCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGGCCTGgcgcg                                  >  2:326101/1‑148 (MQ=255)
                                                                                                              aaCCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCa                                <  7:72910/148‑1 (MQ=255)
                                                                                                              aaCCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGGCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCAt                               <  3:293861/149‑1 (MQ=255)
                                                                                                                ccGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGgcgc                                   <  8:210602/143‑1 (MQ=255)
                                                                                                                ccGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGgcgc                                   >  7:210602/1‑143 (MQ=255)
                                                                                                                ccGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAg                             <  3:351548/149‑1 (MQ=255)
                                                                                                                       gTCGAAGAAACCCTGAATCTGTTCACCGTTCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAg                       >  5:149989/1‑148 (MQ=255)
                                                                                                                       gTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAg                       >  6:130045/1‑148 (MQ=255)
                                                                                                                          gaagaaACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATa                              >  4:44498/1‑138 (MQ=255)
                                                                                                                          gaagaaACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATa                              <  3:44498/138‑1 (MQ=255)
                                                                                                                          gaagaaACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAgg                   <  5:170279/149‑1 (MQ=255)
                                                                                                                             gaaACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGGCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGa                <  2:118036/149‑1 (MQ=255)
                                                                                                                                  ccTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGAt                                                                        <  6:38314/88‑1 (MQ=255)
                                                                                                                                  ccTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGAt                                                                        >  5:38314/1‑88 (MQ=255)
                                                                                                                                  ccTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGATAACa           <  2:212248/149‑1 (MQ=255)
                                                                                                                                   cTGAAGCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCTACACCGACGCTGGAGAGGAAGTCTGCAACCACTTGCGCCGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGATAACAg          <  6:149989/149‑1 (MQ=255)
                                                                                                                                      aaTCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGATAACAGCgg       >  2:252311/1‑149 (MQ=255)
                                                                                                                                      aaTCTGTGCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGATAACAGCgg       <  3:51162/149‑1 (MQ=255)
                                                                                                                                       aTCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAg                    <  2:194364/135‑1 (MQ=255)
                                                                                                                                       aTCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAg                    >  1:194364/1‑135 (MQ=255)
                                                                                                                                       aTCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGATAACAGCGGt      >  2:195225/1‑149 (MQ=255)
                                                                                                                                        tCTGTTCACCGTGCAGCTCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCAAAAGTAGGGGATAACAGCGGt      >  3:271221/1‑148 (MQ=255)
                                                                                                                                           gTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGATAACAGCGGTGATa  >  5:267301/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                               
ATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGATAACAGCGGTGATA  >  NC_000913/1261337‑1261624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: