Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 895,013 | C→T | 6.7% | P8L (CCG→CTG) | potG → | putrescine ABC transporter ATPase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 895,013 | 0 | C | T | 6.7% | 183.9 / 3.1 | 60 | P8L (CCG→CTG) | potG | putrescine ABC transporter ATPase |
Reads supporting (aligned to +/- strand): ref base C (28/27); new base T (2/2); total (31/29) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-01 |
GTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGC > NC_000913/894869‑895161 | gTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAg < 4:48107/149‑1 (MQ=255) gTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAg < 5:160504/149‑1 (MQ=255) tGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAgg < 6:365796/149‑1 (MQ=255) gACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGc < 8:43376/148‑1 (MQ=255) aCCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCTGCGGGc > 5:263954/1‑147 (MQ=255) aCCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCTGCGGGc > 5:263956/1‑147 (MQ=255) aaGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAAc > 1:297753/1‑149 (MQ=255) gTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCTGCGGGCGagggata < 6:263954/149‑7 (MQ=255) gTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCTGCGGGCGagggata < 6:263956/149‑7 (MQ=255) tGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATCCTGCGCTAGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCACGCCAGCATGCGAAAACCCg > 6:361112/1‑149 (MQ=255) aaGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTa < 8:261228/149‑1 (MQ=255) gAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCt < 2:297753/149‑1 (MQ=255) aataatCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGAc < 5:312311/149‑1 (MQ=255) gTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTAt > 3:286339/1‑146 (MQ=255) gTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTa < 2:175231/145‑1 (MQ=255) gTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTa > 1:175231/1‑145 (MQ=255) aGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAg < 6:362784/144‑1 (MQ=255) aGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAg > 5:362784/1‑144 (MQ=255) gATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGATAACCCGTAAGGCGCTGACGCCGCTATTAGAAATc < 1:222766/148‑1 (MQ=255) tGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGc < 3:89056/149‑1 (MQ=255) cGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAAc < 1:24144/149‑1 (MQ=255) gggCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGAc < 8:226378/149‑1 (MQ=255) gcgcACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGAcc > 8:80906/1‑148 (MQ=255) gcACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCaaa < 7:363089/149‑1 (MQ=255) ccgccgGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGAt > 5:179440/1‑149 (MQ=255) cgccgGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATg < 1:201214/149‑1 (MQ=255) ccgGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCACATCCTACGATGGt > 1:46227/1‑149 (MQ=255) ccgGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCCACGATGGt < 7:80906/149‑1 (MQ=255) cATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCTGTGga < 8:209511/149‑1 (MQ=255) cATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGga > 8:156463/1‑149 (MQ=255) aTTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACACGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGgat < 5:361112/149‑1 (MQ=255) ttATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGgat > 4:100258/1‑148 (MQ=255) tGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGgatgat < 5:311729/148‑1 (MQ=255) gTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGga < 4:2172/142‑1 (MQ=255) gTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGga > 3:2172/1‑142 (MQ=255) gTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGt > 2:70127/1‑148 (MQ=255) tGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGt > 7:203820/1‑147 (MQ=255) cgcTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGc > 3:32074/1‑149 (MQ=255) gcTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGcc < 1:70127/149‑1 (MQ=255) ttGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACACTATCCCTAGCCCTCAGGCGAAAACCCGTAAGGCGCTGAAGCCCCTATTAGAAATCCGCAACCTGAACAAATCCTACGAAGGTCAACATGATGTGGATGATCTCAGCCt > 3:109117/1‑148 (MQ=255) acaTTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGa < 3:162357/99‑1 (MQ=255) acaTTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGa > 4:162357/1‑99 (MQ=255) aTTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGCCCATCTa > 6:312603/1‑149 (MQ=255) aTTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTa > 1:169490/1‑149 (MQ=255) ttCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTa > 7:312946/1‑148 (MQ=255) cGGAGAGCAGCCGTGAATGGCGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTg > 2:268523/1‑148 (MQ=255) gagaGCAGCCGTGAATGGCGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGaaa < 1:268523/149‑1 (MQ=255) agagCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAAt < 7:156463/149‑1 (MQ=255) cagcCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTc > 2:127845/1‑149 (MQ=255) gAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGAAGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCgctgc > 2:373078/1‑149 (MQ=255) tGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTgg < 6:179440/149‑1 (MQ=255) tGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTgg > 7:69044/1‑149 (MQ=255) tGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTgg > 4:317887/1‑149 (MQ=255) cTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCCGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCa > 5:220417/1‑149 (MQ=255) cTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCa > 5:368995/1‑149 (MQ=255) tCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATcc > 8:44204/1‑149 (MQ=255) ccTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCgg < 8:312946/149‑1 (MQ=255) cTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTg < 3:10057/120‑1 (MQ=255) cTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTg > 4:10057/1‑120 (MQ=255) tCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCt > 5:14400/1‑149 (MQ=255) cGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGc < 8:203820/149‑1 (MQ=255) | GTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGTGGATGATGTCAGCCTGACCATCTACAAAGGTGAAATCTTCGCGCTGCTGGGCGCATCCGGCTGTGGC > NC_000913/894869‑895161 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |