| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,153,472 | C→A | 13.7% | intergenic (‑374/+224) | ppc ← / ← argE | phosphoenolpyruvate carboxylase/acetylornithine deacetylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,153,472 | 0 | C | A | 13.7% | 142.5 / 2.9 | 59 | intergenic (‑374/+224) | ppc/argE | phosphoenolpyruvate carboxylase/acetylornithine deacetylase |
| Reads supporting (aligned to +/- strand): ref base C (23/26); new base A (3/6); total (27/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCA > NC_000913/4153327‑4153617 | ccccAAGTAACACCAAAGGTGTAGGTCCGATAACATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGAGACGCGAGCGCCATATACGGACTACAGTTGCGCATCGTTTGAGTCCCAgtcggt > 1:8815/1‑149 (MQ=17) cccAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTgtcggtc < 4:202825/149‑1 (MQ=255) ccAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTgtcggtcg < 4:269782/149‑1 (MQ=255) ccAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTgtcggtcg < 7:66746/149‑1 (MQ=255) aaGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGa < 8:289493/149‑1 (MQ=255) aaCACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCGCTGTCGGTCGGAt > 3:122068/1‑146 (MQ=255) ccAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATgc < 5:350319/149‑1 (MQ=255) cAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATgcg > 7:211561/1‑149 (MQ=255) cAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAAAAGTGACGGATGAGGAGAGAGCTCCTTATACGTCATACACTTGGGAATCGTTTGAGTCACTGTCGCTCTGATAAGATgag > 3:225034/1‑147 (MQ=14) tAGGTCGGATAAGATGCGCAAGTATGGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATc < 2:11668/149‑1 (MQ=38) tAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATc < 3:169872/149‑1 (MQ=38) aggtatgATAAGATGCGCAAGTATGGCATCCGACATTATTGTTGCTCTTGAGTTTGTCAACAGTTTCTGATTGGGCTCGATCTCCTTATCCGTCATACAGTTGGGAATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGTATc < 4:252838/142‑1 (MQ=2) ggATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATcc < 2:67151/149‑1 (MQ=37) gcattaGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGTATGCGGCTCGAGCGCCTTATCCTGACTACAGTTGGGCATCGTTTGATTCACTGTAGGTCGGATAAGATGCGCAAGTATCGCATc < 4:278411/143‑1 (MQ=11) aaGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACa < 8:211561/149‑1 (MQ=37) gATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGa < 6:274834/125‑1 (MQ=35) gATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGa > 5:274834/1‑125 (MQ=35) cgcAAGAATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTg < 5:87888/149‑1 (MQ=37) gcAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGc < 5:170227/149‑1 (MQ=37) aaGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTgtcggtc < 6:182262/110‑1 (MQ=35) aaGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTgtcggtc > 5:182262/1‑110 (MQ=35) gTATAGTATCAGACATTATTGATGCTCTGGAGTTTGGCAACAGTGTCGGATGCGGCTAGATCGCCTTATCCGGCATAAAGTTGGGAATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCa < 2:138025/149‑1 (MQ=11) tCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTg > 8:235628/1‑149 (MQ=37) cATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACattatt < 1:260849/137‑1 (MQ=37) cATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGACGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACattatt > 2:260849/1‑137 (MQ=37) tCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGtt < 6:131219/149‑1 (MQ=37) cGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCTGATTCGGCTCGAGCGCCTTATCCGTCCTAAAGTTGGGCATCTTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTg < 6:149053/149‑1 (MQ=11) cGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGa > 6:258559/1‑126 (MQ=38) cGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGa < 5:258559/126‑1 (MQ=38) gACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTgg < 7:27180/149‑1 (MQ=37) agtGGTGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCTTCCTACAGTTGGGCATCTTTTGAGTCACTGTCGGTCGGATAATATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGAt < 2:8815/147‑1 (MQ=14) aCTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGAt < 3:339081/149‑1 (MQ=39) tGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGa > 1:197559/1‑109 (MQ=40) tGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGa < 2:197559/109‑1 (MQ=40) ggAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTg > 6:174957/1‑131 (MQ=17) gAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATgc < 7:293191/147‑1 (MQ=39) gAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGGCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATgc > 8:293191/1‑147 (MQ=255) tttGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATgc < 4:298517/144‑1 (MQ=39) tttGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATgc > 3:298517/1‑144 (MQ=39) aaCAGTGCCTGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCAGAGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAgcgc < 6:12804/149‑1 (MQ=255) aaCAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCAc > 1:236600/1‑112 (MQ=17) ggcgCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTg < 5:55696/99‑1 (MQ=255) ggcgCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTg > 6:55696/1‑99 (MQ=255) ggcgCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCt < 3:302943/136‑1 (MQ=40) ggcgCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCt > 4:302943/1‑136 (MQ=40) gcgAGCGCCTTATCCGGCCTACAGTTGGGCATCGTGTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGtt < 7:235628/149‑1 (MQ=255) gAGCGCCTTATCCGGCCTACAGTTGGGAATCGTTTTAGTCAATGTCGGTAGGATAAGATGCGCAAGTATCGCATAAGACATTATTGAGGCACTGGAGTTTGGCAACAGTGCCGGATGAGGCGCGAGCGCCTTATCCGGCCTACGGtttg > 8:8548/1‑147 (MQ=14) gcgcCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCg > 8:149980/1‑86 (MQ=18) ccTTATCCGGCCTACAGTTTGGCATCGTGTGAGTAACTGTATTTCGGATAAGATGCGCAAGGATCGCATCCGACATTATTGCGGAACTTGAGTTTGGCAACAGGGCCGGATGCGGCGCGAGAGCCTTATCCGGCCTACGGTTgggggtc > 2:392540/1‑145 (MQ=11) aTCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGc > 8:224471/1‑78 (MQ=255) aTCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGc < 7:224471/78‑1 (MQ=21) cGGCCTAGAGTTGGTCATCTTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAg < 4:122068/149‑1 (MQ=255) ggCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGAGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTTTGAATCGtataat > 2:315389/1‑143 (MQ=11) acTACAGGTGGGCATCTTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCa < 4:242818/148‑1 (MQ=255) ggCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGttt > 1:158728/1‑75 (MQ=39) aTCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGTGCATCGTTTGAGTCACTGTAGGTCGGa > 2:57527/1‑148 (MQ=255) gAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGc > 7:265995/1‑37 (MQ=255) gAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGc < 8:265995/37‑1 (MQ=255) gAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCg > 6:326379/1‑102 (MQ=17) catcctcTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTTGAGTTTGGCTACAGTGCCTGATGCGGCTCGTGCGCCTTATGCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATg < 6:247623/142‑1 (MQ=11) gTCACTGTTGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATgcg > 2:58905/1‑148 (MQ=38) aCTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGgcgc > 7:366726/1‑83 (MQ=18) aCTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGgcgc > 7:361254/1‑83 (MQ=18) aCTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGgcgc < 8:366726/83‑1 (MQ=18) tGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAgca > 8:1988/1‑149 (MQ=38) gtcggtcgGATAAGATGCGCATGTATCGCATCCGACATTATTGCGGCACTGGAGttt < 6:282036/57‑1 (MQ=255) | CCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCA > NC_000913/4153327‑4153617 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |