Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,675,495 G→A 7.3% G89G (GGC→GGT)  yphC ← putative Zn‑dependent NAD(P)‑binding oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,675,4950GA 7.3% 255.6 / 6.5 82G89G (GGC→GGT) yphCputative Zn‑dependent NAD(P)‑binding oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (31/45);  new base A (3/3);  total (34/48)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCCGCCAGCAAGTATTCGGCATGACCGCCGTCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGATATAGTGGACATCGCTTCCGC  >  NC_000913/2675351‑2675643
                                                                                                                                                |                                                                                                                                                    
ttcCGCCAGCAAGTATTCGGCATGACCGCCGTCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCct                                                                                                                                                  >  7:360832/1‑149 (MQ=255)
  ccGCCAGCAAGTATTCGGCATGACCGCCGGCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCctc                                                                                                                                                 <  5:224576/148‑1 (MQ=255)
   cGCCAGCAAGTATTCGGCATGACCGCCGTCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCctct                                                                                                                                                <  8:360832/148‑1 (MQ=255)
   cGCCAGCAAGTATTCGGCATGACCGCCGTCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCtt                                                                                                                                               <  6:43536/149‑1 (MQ=255)
    gCCAGCAAGTATTCGGCATGACCGCCGTCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCttt                                                                                                                                              <  2:122041/149‑1 (MQ=255)
    gCCAGCAAGTATTCGGCATGACCGCCGTCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCtt                                                                                                                                               >  2:381136/1‑148 (MQ=255)
     ccAGCAAGTATTCGGCATGACCGCCGTCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCttt                                                                                                                                              >  1:140463/1‑148 (MQ=255)
      cAGGAAGTTTTCGGCATGTCCGCGGTCACGCTGCCAGACGTAAGCCGCTTTTCCTTCGCCAGTACATGTAATTGGAAAACCGCGTCCGGAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACTCGGTCGCCCTCTTTaa                                                                                                                                            <  6:309604/149‑1 (MQ=255)
       aGCGAGTATTCGGCATGACCGCCGTCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTaa                                                                                                                                            >  1:360200/1‑148 (MQ=255)
         cAAGTATTCGGCATGACCGCCGTCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAAt                                                                                                                                         <  3:145419/149‑1 (MQ=255)
         cAAGTATTCGGCATGACCGCCGTCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAAt                                                                                                                                         >  4:150771/1‑149 (MQ=255)
             tATTCGGCATGACCGCCGTCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGCCAGTCCAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCAAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATgtcg                                                                                                                                     <  1:92410/149‑1 (MQ=255)
              aTTCGGCATGACCGCCGTCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACCGCCAGAAATGTGATACCCCAGCACGCGGTCGCGCTCGTTAACATggcgg                                                                                                                                    >  1:157407/1‑149 (MQ=255)
                   gCATGACCGCCGTCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGcc                                                                                                                               >  1:65261/1‑149 (MQ=255)
                     aTGACCGCCGTCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCtt                                                                                                                             <  5:243287/149‑1 (MQ=255)
                     aTGACCGCCGTCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCtt                                                                                                                             >  1:95754/1‑149 (MQ=255)
                      tGACCGCCGTCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTg                                                                                                                            >  1:87956/1‑149 (MQ=255)
                        aCCGCCGTCACGCTGCCAGCCGTAAGCCTCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCGAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGc                                                                                                                           <  6:163009/148‑1 (MQ=255)
                        aCCGCCGTCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGc                                                                                                                           <  8:274755/148‑1 (MQ=255)
                        aCCGCCGTCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGc                                                                                                                           <  3:182510/148‑1 (MQ=255)
                            ccgTCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCAt                                                                                                                      >  2:260073/1‑149 (MQ=255)
                            ccgTCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATACGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCAt                                                                                                                      <  2:188149/149‑1 (MQ=255)
                               tCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGc                                                                                                                   <  1:151062/149‑1 (MQ=255)
                               tCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGc                                                                                                                   <  8:248588/149‑1 (MQ=255)
                               tCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGc                                                                                                                   >  7:248588/1‑149 (MQ=255)
                                     tGCCAGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCcacatt                                                                                                             >  2:352797/1‑147 (MQ=255)
                                       ccAGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAAt                                                                                                             <  1:193779/147‑1 (MQ=255)
                                       ccAGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTAGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAAt                                                                                                             <  4:319811/147‑1 (MQ=255)
                                              tAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGACGCCCTCTTTAAAATGGCGGCAGCCTTGCCCAAACGCCACAATCTGCCCGCa                                                                                                    >  4:13038/1‑149 (MQ=255)
                                                    gCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCca                                                                                                                 <  2:368013/130‑1 (MQ=255)
                                                    gCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCca                                                                                                                 >  1:368013/1‑130 (MQ=255)
                                                     cTTTTCCTTCGCCAGTACAAGAAATAGGGAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCa                                                                                             >  1:128163/1‑149 (MQ=255)
                                                     cTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCa                                                                                             >  5:197969/1‑149 (MQ=255)
                                                        ttCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCAt                                                                                            <  3:150771/147‑1 (MQ=255)
                                                          ccTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGAcc                                                                                        >  4:196697/1‑149 (MQ=255)
                                                           cTTCGCCAGTACAAGAAATAGGAAAACCGCGACTGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGAcc                                                                                        <  4:252664/148‑1 (MQ=255)
                                                           cTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGAcc                                                                                        <  2:95754/148‑1 (MQ=255)
                                                           cTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCg                                                                                       >  2:148174/1‑149 (MQ=255)
                                                              cGCCAGTACAAGAAATAGGGAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGtt                                                                                     <  2:128163/148‑1 (MQ=255)
                                                              cGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGtt                                                                                     <  2:140463/148‑1 (MQ=255)
                                                              cGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGt                                                                                      <  7:240764/147‑1 (MQ=255)
                                                               gCCAGTACAAGAAATAGGAAAACCGCGCCGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCACCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGa                                                                                   >  2:322131/1‑149 (MQ=255)
                                                               gCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCTGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCACCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGa                                                                                   >  8:382352/1‑149 (MQ=255)
                                                               gCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCTCAGCCAGAAATGTGATACACCAGCACGCGGGCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTg                                                                                    <  6:133659/148‑1 (MQ=255)
                                                               gCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCCCGCGGTCACCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTg                                                                                    >  8:387675/1‑148 (MQ=255)
                                                                ccAGTACAAGAAATAGGAAAACCGCGACGGCAGTACGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGa                                                                                   >  2:328850/1‑148 (MQ=255)
                                                                 cAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACCCCAGCACGCGGTCGCCCGCTTTAAAATGGCGGCGGCCTGGCCCCATCGCCACAAGCGGCCCGCCCGGTTCATGACCGtt                                                                                     >  5:123126/1‑145 (MQ=255)
                                                                    tACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAg                                                                              >  1:367789/1‑149 (MQ=255)
                                                                    tACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAg                                                                              >  4:78761/1‑149 (MQ=255)
                                                                      cAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGc                                                                             <  6:371583/148‑1 (MQ=255)
                                                                         gAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTg                                                                         <  1:260073/149‑1 (MQ=255)
                                                                         gAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTg                                                                         >  4:65041/1‑149 (MQ=255)
                                                                           aaTAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGt                                                                       <  2:87956/149‑1 (MQ=255)
                                                                             tAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATATGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGtat                                                                     <  4:218924/149‑1 (MQ=255)
                                                                                          aCGGCCGGTCGGGCACAAACCACAGCCAGAACTGTGCGACACCACCACGCGGTCGCCCTCTTTAAAATGGCGGCATCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCgg                                                        <  5:240525/149‑1 (MQ=255)
                                                                                          aCGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCgg                                                        <  2:192778/149‑1 (MQ=255)
                                                                                            ggCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGt                                                      >  4:223400/1‑149 (MQ=255)
                                                                                                    gggCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGccgccg                                               <  6:197969/148‑1 (MQ=255)
                                                                                                       cAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCtgt                                           <  2:395657/149‑1 (MQ=255)
                                                                                                           aaCCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCa                                       <  3:196697/149‑1 (MQ=255)
                                                                                                             ccACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACg                                     >  1:348869/1‑149 (MQ=255)
                                                                                                                caGCCAGAAATGTGATACACCAGCCCGCGGTCACCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGgtg                                  <  7:387675/149‑1 (MQ=255)
                                                                                                                caGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGgtg                                  <  2:348869/149‑1 (MQ=255)
                                                                                                                caGCCAGAAATGTGATACACCAGCACGCGGTCACCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGgtg                                  <  7:382352/149‑1 (MQ=255)
                                                                                                                caGCCAGAAATGTGATACACCAGCACGCGGTCACCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGGGCCGCCGCTGTGGCACGgtg                                  <  1:322131/149‑1 (MQ=255)
                                                                                                                 aGCCAGAAATTTGATACACCAGCACGCGGTAGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGgtgt                                 <  2:98840/149‑1 (MQ=255)
                                                                                                                  gCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGtt                                <  1:328850/149‑1 (MQ=255)
                                                                                                                  gCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTCAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGtt                                <  7:26215/149‑1 (MQ=255)
                                                                                                                  gCCAGAAATGTGATACACCAGCACGCGGGCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTATGGCACGGTGtt                                <  8:274386/149‑1 (MQ=255)
                                                                                                                     aGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTtgat                             >  5:318318/1‑149 (MQ=255)
                                                                                                                         aTGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTagatga                           >  6:173538/1‑147 (MQ=255)
                                                                                                                         aTGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATgata                         <  2:367789/149‑1 (MQ=255)
                                                                                                                           gtgATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTgg                                                                        >  6:57677/1‑100 (MQ=255)
                                                                                                                           gtgATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTgg                                                                        <  5:57677/100‑1 (MQ=255)
                                                                                                                            tgATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATgatagat                      <  3:78761/149‑1 (MQ=255)
                                                                                                                            tgATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATgataga                       >  5:324511/1‑148 (MQ=255)
                                                                                                                                 cacCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGATATAg                  <  1:352797/148‑1 (MQ=255)
                                                                                                                                  acCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGATATAGTg                <  3:320222/149‑1 (MQ=255)
                                                                                                                                     aGCACGCGGTAGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGATATAGTGGa              >  2:49326/1‑148 (MQ=255)
                                                                                                                                       cACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGATATAGTGGACAt           >  4:260088/1‑149 (MQ=255)
                                                                                                                                            ggTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGATATAGTGGACATCGCtt      <  3:223400/149‑1 (MQ=255)
                                                                                                                                             gTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTTGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGATATAGTGGACATCGCTTc     <  1:381136/149‑1 (MQ=255)
                                                                                                                                                gCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGATATAGTGGACATCGCTTCCGc  <  6:318318/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
TTCCGCCAGCAAGTATTCGGCATGACCGCCGTCACGCTGCCAGCCGTAAGCCGCTTTTCCTTCGCCAGTACAAGAAATAGGAAAACCGCGACGGCAGTTCGGGCAAAAACCACAGCCAGAAATGTGATACACCAGCACGCGGTCGCCCTCTTTAAAATGGCGGCAGCCTTGCCCCATCGCCACAATCTGCCCGCACGGTTCATGACCGTTGATAAAGCCCTGGTATAACGGTTTATCGGGTGCCGCCGCTGTGGCACGGTGTTGATGATAGATATAGTGGACATCGCTTCCGC  >  NC_000913/2675351‑2675643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑