Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,324,626 T→C 5.4% V173A (GTC→GCC)  atoA → acetyl‑CoA:acetoacetyl‑CoA transferase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,324,6260TC 5.4% 224.1 / 2.9 74V173A (GTC→GCC) atoAacetyl‑CoA:acetoacetyl‑CoA transferase, beta subunit
Reads supporting (aligned to +/- strand):  ref base T (37/33);  new base C (2/2);  total (39/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.98e-01

CGATGGATCTGGTGACCGGGTCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGATCTGAATACGCAACGGGGTGATTTATGATTGGTCGCATATC  >  NC_000913/2324482‑2324772
                                                                                                                                                |                                                                                                                                                  
cgATGGATCTGGTGACCGGGTCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCttt                                                                                                                                                >  7:309403/1‑149 (MQ=255)
      aTCTGGTGACCGGGTCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGttt                                                                                                                                           >  1:333799/1‑148 (MQ=255)
      aTCTGGTGACCGGGTCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGttt                                                                                                                                           >  2:350102/1‑148 (MQ=255)
           gTGACCGGGTCGCGCAAAGTGATCATCGCCATTGAAAAATGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGACCTGGCTGTCTTTCGTTTTAt                                                                                                                                        <  6:275863/146‑1 (MQ=255)
           gTGACCGGGTCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGtttt                                                                                                                                          <  2:367366/144‑1 (MQ=255)
           gTGACCGGGTCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGa                                                                                                                                     >  8:369030/1‑149 (MQ=255)
            tGACCGGGTCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGAc                                                                                                                                    <  5:74553/149‑1 (MQ=255)
              cccGGGTCGCGCAATGGGATCATCGGCATGGAACTTTGCGCCAAAGAGGCTTCAGCAAAAATATTGCGCCCCGGGCCCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTCTACTGGCTGTCTTTCGTTTTATTGACgg                                                                                                                                  <  8:176108/148‑1 (MQ=255)
              aCCTGGGCGCGCCAAGTGAGCCTCGCCATGGAACATTGCGCCAAAGAGGGTGCAGCAAAAAGTTTGCGCCGCTGCACCATGCAACTCACTTCGCAACATGAGTTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACgg                                                                                                                                  <  8:181483/149‑1 (MQ=255)
                       cgcAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAAtgtg                                                                                                                         <  4:27246/149‑1 (MQ=255)
                        gcAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTgg                                                                                                                        <  7:80372/149‑1 (MQ=255)
                        gcAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGATCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGCCTTTCGTTTTATTGACGGCAAAAtgtg                                                                                                                         >  6:93970/1‑148 (MQ=255)
                        gcAAAGTGATCATCGCCATGGAACATTGAGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCAACTCACTGCGCAACATGAGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTgg                                                                                                                        >  7:345293/1‑149 (MQ=255)
                         cAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATTCCACTCACTGCGCAACACGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGc                                                                                                                       >  3:256440/1‑149 (MQ=255)
                                atcatcGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCg                                                                                                                 >  7:2554/1‑148 (MQ=255)
                                   atcGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGaaa                                                                                                              <  8:59072/148‑1 (MQ=255)
                                   atcGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAAt                                                                                                             >  5:75449/1‑149 (MQ=255)
                                   atcGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAAt                                                                                                             >  4:179432/1‑149 (MQ=255)
                                          tGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGAc                                                                                                      >  8:176393/1‑149 (MQ=255)
                                            gAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACCGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACgg                                                                                                    <  1:374596/149‑1 (MQ=255)
                                            gAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACCGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACgg                                                                                                    >  2:374596/1‑149 (MQ=255)
                                               cATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGgtg                                                                                                 <  4:330513/149‑1 (MQ=255)
                                                    cgcCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGAt                                                                                             <  7:176393/148‑1 (MQ=255)
                                                    cgcCAAAGATGGGTCAGCAAAAATTTTGCGCCACTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGAt                                                                                             <  2:147512/148‑1 (MQ=255)
                                                       cAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAg                                                                                         >  7:215198/1‑149 (MQ=255)
                                                       cAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAg                                                                                         >  1:183634/1‑149 (MQ=255)
                                                        aaaGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGGTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGc                                                                                        >  8:164332/1‑149 (MQ=255)
                                                           gATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGAtt                                                                                            <  7:41806/142‑1 (MQ=255)
                                                           gATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGAtt                                                                                            >  8:41806/1‑142 (MQ=255)
                                                             tGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACcg                                                                                   >  6:105770/1‑149 (MQ=255)
                                                               gTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGGGTGATTTAGCCACcgt                                                                                  <  5:50942/148‑1 (MQ=255)
                                                                    gCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCgtgcgtgc                                                                            >  2:65844/1‑149 (MQ=255)
                                                                       aaaaTTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAATTGTGGCTCAGGGAAATTGCCGACGGGTGTGATTTAGCCACcgtgattggca                                                                          >  1:314745/1‑141 (MQ=255)
                                                                       aaaaTTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCaa                                                                         <  3:179432/149‑1 (MQ=255)
                                                                       aaaaTTTTGCGCCGCTGCACCATGCCACTCACTGAGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGTTTTAGCCTCCGTGCGTGCCa                                                                          >  8:149225/1‑148 (MQ=255)
                                                                           ttttGCGCCGCTGCACCCTGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCaaaa                                                                       >  7:40416/1‑147 (MQ=255)
                                                                           ttttGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACa                                                                     <  1:369061/149‑1 (MQ=255)
                                                                                cgccgcTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGc                                                                <  8:40416/149‑1 (MQ=255)
                                                                                 gccgcTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCt                                                               >  1:188997/1‑149 (MQ=255)
                                                                                  ccgcTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGCCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTc                                                              <  5:93970/149‑1 (MQ=255)
                                                                                   cgcTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCg                                                             >  7:260823/1‑149 (MQ=255)
                                                                                    gcTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGCCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACcgt                                                                                  >  2:264875/1‑127 (MQ=255)
                                                                                    gcTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGCCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACcgt                                                                                  <  1:264875/127‑1 (MQ=255)
                                                                                     cTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGt                                                           <  2:183634/149‑1 (MQ=255)
                                                                                          cgaTGCCACTGACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCTGTTTGaa                                                      <  1:57855/147‑1 (MQ=255)
                                                                                             tGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTc                                                   >  7:169684/1‑149 (MQ=255)
                                                                                                cactcactGCGCAACATGCGGTGCATACGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTcgcc                                                >  4:269749/1‑149 (MQ=255)
                                                                                                  ctcactGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTcgccgc                                              <  6:75449/149‑1 (MQ=255)
                                                                                                     actGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGa                                           <  7:369030/149‑1 (MQ=255)
                                                                                                     actGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGa                                           >  1:187971/1‑149 (MQ=255)
                                                                                                      ctGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGGGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGAt                                          <  8:309403/149‑1 (MQ=255)
                                                                                                        gcgcAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGATCt                                        >  8:71218/1‑149 (MQ=255)
                                                                                                           cAACATGCGGTGCATATGCTGGGTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAAAGTGGCTCACCGAAATGGCCGACGGGTGTGAGTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGATCTGaa                                     <  2:301203/149‑1 (MQ=255)
                                                                                                             aCATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGC‑AAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCTGTTTGAAGTCGCCGCCGATCTGAATAc                                  <  1:400744/149‑1 (MQ=255)
                                                                                                              cATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGATCTGAATAc                                  <  8:2554/149‑1 (MQ=255)
                                                                                                                    gTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGATCTGAATACGCAACg                            >  8:48980/1‑149 (MQ=255)
                                                                                                                    gTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGATCTGAATACGCAACg                            >  8:43023/1‑149 (MQ=255)
                                                                                                                       cATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGATCTGAATACGCAACgggg                         <  2:188997/149‑1 (MQ=255)
                                                                                                                        atacGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGATCTGAATACGCAACgggg                         <  3:269749/148‑1 (MQ=255)
                                                                                                                         tatGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGATCTGAATACGCAACGGGGt                        >  3:369990/1‑148 (MQ=255)
                                                                                                                           tGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGATCTGAATACGCAACGGGGTGAt                     >  8:90358/1‑149 (MQ=255)
                                                                                                                              tGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGATCTGAATACGCAACggg                          >  2:368743/1‑141 (MQ=255)
                                                                                                                              tGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGATCTGAATACGCAACggg                          <  1:368743/141‑1 (MQ=255)
                                                                                                                              tGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGATCTGAATACGCAACGGGGTGATTTa                  >  8:286963/1‑149 (MQ=255)
                                                                                                                              tGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGATCTGAATACGCAACGGGGTGATTTa                  >  4:185067/1‑149 (MQ=255)
                                                                                                                               ggTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGATCTGAATACGCAACGGGGTGATTTAt                 >  6:269634/1‑149 (MQ=255)
                                                                                                                                 ttACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGATCTGAATACGCAACGGGGTGATTTATGa               <  8:338289/149‑1 (MQ=255)
                                                                                                                                    ctccaCTGGCTGTCGCTCGTTTTATTGCCGGCAAAACGAGGCTCACCCAACTTGCCGACCGGTGCCATCTAGCCACCGTGCGTGCCAAAACCGAAGCGCGCCTTGCAGTCGCCGCCGATCTGAACACGCAACGCGGTGATATATGATTg            <  2:333799/145‑1 (MQ=255)
                                                                                                                                    cTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGATCTGAATACGCAACGGGGTGATTTATGATTg            >  4:274096/1‑149 (MQ=255)
                                                                                                                                       aaCTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGATCTGAATACGCCACGGGGTGATTTATGATTGGTc         >  2:228275/1‑149 (MQ=255)
                                                                                                                                       aaCTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGATCTGAATACGCAACGGGGTGATTTATGATTGGTc         <  7:90358/149‑1 (MQ=255)
                                                                                                                                       aaCTGGCGGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGGGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGATCTGAATACGCAACGGGGGGATTTATGATTGGTc         <  2:85973/149‑1 (MQ=255)
                                                                                                                                          tGGCTGTCTTTCGATTTATTGACGGCAAAATGTGGCTCCCCGAAATTGCCGACGGGTCTTATTTAGCCACCGTCCGTCCCAAAACAGAAGCTCGGTTTTAAGTCGCCGCCGATCTGAAAACGCAACGGTGTGATTTATGATTGTTCGc       >  4:220835/1‑148 (MQ=255)
                                                                                                                                              tGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAAATGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGATCTGAATACGCAACGGGGTGATTTATGATTGGTCGCATATc  <  7:149225/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CGATGGATCTGGTGACCGGGTCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGATCTGAATACGCAACGGGGTGATTTATGATTGGTCGCATATC  >  NC_000913/2324482‑2324772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑