Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,305,087 C→T 5.2% intergenic (+694/+21) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,305,0870CT 5.2% 313.1 / 4.8 95intergenic (+694/+21)eco/mqoecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base C (46/44);  new base T (2/3);  total (48/47)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01

CGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGC  >  NC_000913/2304940‑2305235
                                                                                                                                                   |                                                                                                                                                    
cgcgTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCa                                                                                                                                                     <  1:226146/149‑1 (MQ=255)
         tCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATGGTATGCCGGAt                                                                                                                                             <  8:251550/148‑1 (MQ=255)
           aGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGAt                                                                                                                                             <  6:212067/146‑1 (MQ=255)
           aGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGAt                                                                                                                                             >  5:212067/1‑146 (MQ=255)
             gCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGc                                                                                                                                        <  1:253097/149‑1 (MQ=255)
             gCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGc                                                                                                                                        <  4:81812/149‑1 (MQ=255)
             gCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGc                                                                                                                                        >  2:253097/1‑149 (MQ=255)
                tACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATGGTATGCCGGAtgtg                                                                                                                                          <  4:213577/144‑1 (MQ=255)
                tACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATGGTATGCCGGAtgtg                                                                                                                                          >  3:213577/1‑144 (MQ=255)
                   acCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCa                                                                                                                                  >  6:189505/1‑149 (MQ=255)
                   acCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCa                                                                                                                                  >  4:141643/1‑149 (MQ=255)
                   acCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCa                                                                                                                                  >  4:136268/1‑149 (MQ=255)
                     cGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTACCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCAt                                                                                                                                 >  7:338387/1‑148 (MQ=255)
                               tGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCaa                                                                                                                       <  8:256187/148‑1 (MQ=255)
                               tGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAat                                                                                                                      <  8:257022/149‑1 (MQ=255)
                                gCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCCTATCATTACAACGCaaaa                                                                                                                     >  8:75282/1‑147 (MQ=255)
                                   ccACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATGGTATGCCGGATGTGGCGTAtgc                                                                                                                                  <  1:77020/133‑3 (MQ=255)
                                   ccACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATGGTATGCCGGATGTGGCGTAtgc                                                                                                                                  >  2:77020/1‑131 (MQ=255)
                                   ccACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACACCGCAATATcc                                                                                                                  >  1:237479/1‑149 (MQ=255)
                                     aCCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGc                                                                                                                <  2:214947/149‑1 (MQ=255)
                                         tagatgTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCAct                                                                                                            >  7:264976/6‑149 (MQ=255)
                                         cGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCAct                                                                                                            >  4:100326/1‑149 (MQ=255)
                                              gTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCActtctt                                                                                                        <  4:290313/148‑1 (MQ=255)
                                              gTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCttt                                                                                                       >  1:386316/1‑149 (MQ=255)
                                               tggtaTACGGCTGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCtttt                                                                                                      <  7:304994/145‑1 (MQ=255)
                                                tGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCttttt                                                                                                     >  1:237260/1‑149 (MQ=255)
                                                  gATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGa                                                                                                   <  2:386316/149‑1 (MQ=255)
                                                     aaGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCa                                                                                                              <  2:128661/135‑1 (MQ=255)
                                                     aaGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCa                                                                                                              >  1:128661/1‑135 (MQ=255)
                                                     aaGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACg                                                                                                >  8:148476/1‑149 (MQ=255)
                                                        gCGGTTACGCCACATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGt                                                                                             >  3:18728/1‑149 (MQ=255)
                                                           gTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGc                                                                                                                                                  <  3:262401/93‑1 (MQ=255)
                                                           gTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGc                                                                                                                                                  >  4:262401/1‑93 (MQ=255)
                                                           gTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGc                                                                                          <  3:141643/149‑1 (MQ=255)
                                                           gTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGc                                                                                          <  3:136268/149‑1 (MQ=255)
                                                            ttACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCg                                                                                         >  2:143720/1‑149 (MQ=255)
                                                                gccgcATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTa                                                                                                                                     >  5:185356/1‑101 (MQ=255)
                                                                gccgcATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTa                                                                                                                                     <  6:185356/101‑1 (MQ=255)
                                                                   gcATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCaa                                                                                  >  5:270165/1‑149 (MQ=255)
                                                                        cAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCTATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCg                                                                             <  1:159933/149‑1 (MQ=255)
                                                                         aaCATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCgg                                                                            >  5:56217/1‑149 (MQ=255)
                                                                         aaCATCTAACGCCCGAGCCGGTTGCCTGATGAGACGCTTGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCgg                                                                            >  5:80837/1‑149 (MQ=255)
                                                                           cATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGtt                                                                          <  3:100326/149‑1 (MQ=255)
                                                                            aTCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGttt                                                                         <  1:322555/149‑1 (MQ=255)
                                                                            aTCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGAAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGttt                                                                         >  6:364334/1‑149 (MQ=255)
                                                                               tAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCgg                                                                      >  3:361741/1‑149 (MQ=255)
                                                                               tAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCgg                                                                      >  3:356028/1‑149 (MQ=255)
                                                                                aaCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGc                                                                     >  8:267357/1‑149 (MQ=255)
                                                                                 aCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACt                                                                                >  3:325618/1‑137 (MQ=255)
                                                                                 aCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACt                                                                                <  4:325618/137‑1 (MQ=255)
                                                                                   gCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTAc                                                                                                                              >  4:202064/1‑89 (MQ=255)
                                                                                   gCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCAGACCATGCCGGATGTGGCGTATCATTAc                                                                                                                              <  3:202064/89‑1 (MQ=255)
                                                                                     ccGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGTGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGttt                                                                         <  2:172624/140‑1 (MQ=255)
                                                                                     ccGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGTGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGttt                                                                         >  1:172624/1‑140 (MQ=255)
                                                                                     ccGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTAc                                                                 >  6:110774/1‑148 (MQ=255)
                                                                                            ggTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATctgct                                                         <  2:237260/149‑1 (MQ=255)
                                                                                             gTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATctgctg                                                        <  8:387238/149‑1 (MQ=255)
                                                                                              ttGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCtgctgc                                                       <  7:331225/149‑1 (MQ=255)
                                                                                                 ccTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGctgcctg                                                    >  8:5038/1‑149 (MQ=255)
                                                                                                   tGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCg                                                  >  2:219992/1‑149 (MQ=255)
                                                                                                   tGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCg                                                  >  3:81649/1‑149 (MQ=255)
                                                                                                         gACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTa                                                               >  4:90758/1‑130 (MQ=255)
                                                                                                         gACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTa                                                               <  3:90758/130‑1 (MQ=255)
                                                                                                         gACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATctg                                                           <  3:158636/134‑1 (MQ=255)
                                                                                                         gACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATctg                                                           >  4:158636/1‑134 (MQ=255)
                                                                                                         gACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGctgcc                                                      <  6:386792/139‑1 (MQ=255)
                                                                                                         gACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGctgcc                                                      >  5:386792/1‑139 (MQ=255)
                                                                                                           cGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCg                                          <  1:380663/149‑1 (MQ=255)
                                                                                                                gcgcGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAg                                       <  4:81649/147‑1 (MQ=255)
                                                                                                                gcgcGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAg                                       <  5:189505/147‑1 (MQ=255)
                                                                                                                  gcgTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTc                                    >  8:290509/1‑148 (MQ=255)
                                                                                                                     tCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCAGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAAcc                                >  6:205308/1‑149 (MQ=255)
                                                                                                                        tATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAAccc                               >  3:212614/1‑147 (MQ=255)
                                                                                                                         aTCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCAGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAAccc                               <  5:205308/146‑1 (MQ=255)
                                                                                                                          tCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAg                            <  7:148476/148‑1 (MQ=255)
                                                                                                                            aGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCa                          <  4:356028/148‑1 (MQ=255)
                                                                                                                            aGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCa                          <  4:361741/148‑1 (MQ=255)
                                                                                                                             gCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCaa                                                                                  >  5:142147/1‑91 (MQ=255)
                                                                                                                             gCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCaa                                                                                  <  6:142147/91‑1 (MQ=255)
                                                                                                                             gCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAAc                                 >  8:68843/1‑140 (MQ=255)
                                                                                                                             gCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAAc                                 <  7:68843/140‑1 (MQ=255)
                                                                                                                                 aCGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGc                    >  1:341045/1‑149 (MQ=255)
                                                                                                                                   gCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTg                  <  6:270165/149‑1 (MQ=255)
                                                                                                                                   gCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTg                  <  8:159/149‑1 (MQ=255)
                                                                                                                                   cccACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTg                  <  2:341045/148‑1 (MQ=255)
                                                                                                                                     cacaTCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGgt                <  4:18728/149‑1 (MQ=255)
                                                                                                                                     cacaTCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGgt                >  6:187493/1‑149 (MQ=255)
                                                                                                                                        aTCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTa             >  2:159739/1‑149 (MQ=255)
                                                                                                                                        aTCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTa             <  1:129032/149‑1 (MQ=255)
                                                                                                                                           cGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCTTAGTTCAACCCCAGCACTTCGCTGGTGTACTg          <  7:267357/149‑1 (MQ=255)
                                                                                                                                            ggCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTa                                                                  <  2:56956/92‑1 (MQ=255)
                                                                                                                                            ggCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTa                                                                  >  1:56956/1‑92 (MQ=255)
                                                                                                                                             gCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGc         >  4:179328/1‑148 (MQ=255)
                                                                                                                                              cATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTGCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCaa       <  2:237479/149‑1 (MQ=255)
                                                                                                                                                 aCCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTc    >  7:55330/1‑149 (MQ=255)
                                                                                                                                                   cATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTcgc  >  4:333150/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTCGC  >  NC_000913/2304940‑2305235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: