Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,555,157 A→G 5.4% I171I (ATT→ATC maeA ← malate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,555,1570AG 5.4% 231.9 / 2.9 75I171I (ATT→ATCmaeAmalate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme
Reads supporting (aligned to +/- strand):  ref base A (31/39);  new base G (2/2);  total (34/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

GTCAGTGATACGCGGATTACGCCAGCCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACTTTAATATTATGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGT  >  NC_000913/1555010‑1555304
                                                                                                                                                   |                                                                                                                                                   
gtcAGTGATACGCGGATTACGCCAGCCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGaa                                                                                                                                                    <  6:321868/149‑1 (MQ=255)
 tcAGTGATACGCGGATTACGCCAGCCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAAt                                                                                                                                                   <  3:325166/149‑1 (MQ=255)
 tcAGTGATACGCGGATTACGCCAGCCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAAt                                                                                                                                                   <  2:283444/149‑1 (MQ=255)
   atgttatcgcgGTTTAAGCCATCCCATAAACACCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGc                                                                                                                                                 <  3:178998/142‑1 (MQ=255)
    gTGATACGCGGATTCCGCCAGCCCATATCCAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCTCCACAGGTAGGGTATACGCCGGGCTGATGCGGCCACAGGCGGTATAGAGCGACAGTTTACCGCTCGGAATGcc                                                                                                                                                <  6:68732/149‑1 (MQ=255)
    gTGATACGCGGATTACGCCAGCCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGcc                                                                                                                                                >  3:5179/1‑149 (MQ=255)
          cgcgGATTAAGCCAGCCCATATACATCGGATCGTTAAGGAGCTGTTTGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATccc                                                                                                                                          <  4:239584/149‑1 (MQ=255)
              gATTACGCCAGCCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGCTCGGAATGCCCATCacgcc                                                                                                                                       >  8:146895/1‑148 (MQ=255)
              gATTACGCCAGCCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCccgcc                                                                                                                                       >  8:278361/1‑148 (MQ=255)
                ttACGCCAGCCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGAt                                                                                                                                    <  7:369408/149‑1 (MQ=255)
                     ccAGCCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCt                                                                                                                               <  1:97546/149‑1 (MQ=255)
                      cAGCCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTg                                                                                                                              >  1:87208/1‑149 (MQ=255)
                        gCCCATATACAGCGGTTCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTg                                                                                                                              <  8:259046/147‑1 (MQ=255)
                        gCCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTg                                                                                                                              >  7:259046/1‑147 (MQ=255)
                           cATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGGATGCCCATCCCGCCGATGCCCTGGTCAc                                                                                                                         >  5:370994/1‑149 (MQ=255)
                           cATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGGATGCCCATCCCGCCGATGCCCTGGTCAc                                                                                                                         >  3:307155/1‑149 (MQ=255)
                           cATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCAc                                                                                                                         >  7:347755/1‑149 (MQ=255)
                            agataCAGCGGAGCGTTAAGCAGCGGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCGGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCAcc                                                                                                                        <  6:191362/147‑1 (MQ=255)
                                 cAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGcc                                                                                                                   >  2:316813/1‑149 (MQ=255)
                                  aGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGccc                                                                                                                  >  8:275325/1‑149 (MQ=255)
                                   gCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGcccc                                                                                                                 <  4:84450/149‑1 (MQ=255)
                                      gATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAg                                                                                                               >  2:28980/1‑148 (MQ=255)
                                                 agcTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCAcc                                                                                                   >  4:345583/1‑149 (MQ=255)
                                                      ttggttgTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGGATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCgtca                                                                                               <  6:370994/148‑1 (MQ=255)
                                                      ttggttgTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGGATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCgtca                                                                                               <  4:307155/148‑1 (MQ=255)
                                                      ttggttgTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCGTCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCgtcag                                                                                              >  2:41867/1‑149 (MQ=255)
                                                      ttggttgTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCgtca                                                                                               <  1:116848/148‑1 (MQ=255)
                                                      ttggttgTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCgtca                                                                                               <  4:49643/148‑1 (MQ=255)
                                                         gttgttCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCgtcagaca                                                                                           >  7:395507/1‑149 (MQ=255)
                                                              tcgttcCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTcaccac                                                                                       >  8:249593/1‑148 (MQ=255)
                                                                gttcCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACaa                                                                                     <  7:249593/148‑1 (MQ=255)
                                                                    cgccATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACt                                                                                >  7:229692/4‑149 (MQ=255)
                                                                    cGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACt                                                                                >  7:121535/1‑149 (MQ=255)
                                                                    cGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACt                                                                                <  4:5179/149‑1 (MQ=255)
                                                                    cGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACt                                                                                >  2:334057/1‑149 (MQ=255)
                                                                     gACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACtt                                                                               <  7:278361/149‑1 (MQ=255)
                                                                        aTCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACTTTaa                                                                            <  1:146727/149‑1 (MQ=255)
                                                                          ccAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACGGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACTTTAata                                                                          >  2:49762/1‑149 (MQ=255)
                                                                             gCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACTTTAAtatta                                                                       <  3:368712/149‑1 (MQ=255)
                                                                              caccaaCTGAAGGGTATACGCCGGGCTGATGCCGACACAGGCGGTATATATCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAAGGCGTTCACCGTCAGTCACCACAATCACTTTAAtattat                                                                      <  6:241563/149‑1 (MQ=255)
                                                                                 caccGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACTTTAATATTATGGt                                                                   <  3:345583/149‑1 (MQ=255)
                                                                                 caccGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACTTTAATATTATGGt                                                                   >  2:96000/1‑149 (MQ=255)
                                                                                            tataCGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCgtca                                                                                               >  4:236688/1‑110 (MQ=255)
                                                                                            tataCGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCgtca                                                                                               <  3:236688/110‑1 (MQ=255)
                                                                                                cGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACTTTAATATTATGGTTCGGCACGTTTTGc                                                     <  1:96000/148‑1 (MQ=255)
                                                                                                 gCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGCTCGGCATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACTTTAATATTATGGTTCGGCACGTTTTg                                                      >  7:351963/1‑146 (MQ=255)
                                                                                                 gCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACTTTAATATTATGGTTCGGCACGTTTTg                                                      >  7:95146/1‑146 (MQ=255)
                                                                                                    gggCTGATGCCGGCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACTTTAATATTATGGTTCGGCACGTTTTGCAGAat                                                <  7:146895/149‑1 (MQ=255)
                                                                                                    gggCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCgtcagt                                                                                             >  4:69399/1‑104 (MQ=255)
                                                                                                    gggCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCgtcagt                                                                                             <  3:69399/104‑1 (MQ=255)
                                                                                                        tGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACTTTAAtatt                                                                        <  4:23182/121‑1 (MQ=255)
                                                                                                        tGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACTTTAAtatt                                                                        >  3:23182/1‑121 (MQ=255)
                                                                                                          aTGCCGCCACAGGCGGTATAGAGCGACCGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACTTTAATATTATGGTTCGGCACGTTTTGCAGAATATCGTc                                          <  1:49762/149‑1 (MQ=255)
                                                                                                          aTGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACTTTAATATTATGGTTCGGCACGTTTTGCAGAATATCGTc                                          <  1:316813/149‑1 (MQ=255)
                                                                                                          aTGCCGCCACAGGCGGTATAGAGCGACAGGTGACCGATCGGAATGCCCATCCCGCCGAGGCCCTGGTCACCACGCCCCAGAATGCGGTCACCGTCAGTCACCACACTCCCTTTAATATTATGGTTCGGCACGTTTTGCAGAATATCGTc                                          <  3:304868/149‑1 (MQ=255)
                                                                                                              cgccACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGCCACCAAGCCCCAGAATGCGTTc                                                                                                      >  3:22051/1‑85 (MQ=255)
                                                                                                              cgccACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGCCACCAAGCCCCAGAATGCGTTc                                                                                                      >  3:22054/1‑85 (MQ=255)
                                                                                                              cgccACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGCCACCAAGCCCCAGAATGCGTTc                                                                                                      <  4:22051/85‑1 (MQ=255)
                                                                                                              cgccACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGCCACCAAGCCCCAGAATGCGTTc                                                                                                      <  4:22054/85‑1 (MQ=255)
                                                                                                               gccACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACTTTAATATTATGGTTCGGCACGTTTTGCAGAATATCGTCAatat                                     >  1:60559/1‑149 (MQ=255)
                                                                                                                ccACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACTTTAATATTATGGTTCGGCACGTTTTGCAGAATATCGTCCATAtt                                    <  6:137630/149‑1 (MQ=255)
                                                                                                                ccACAGGCGGTATAGAGCGACAGTGTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACTTTAATATTATGGTTCGGCACGTTTTGCAGAATATCGTCCATAtt                                    <  1:334057/149‑1 (MQ=255)
                                                                                                                 cacaGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACTTTAATATTATGGTTCGGCACGTTTTGCAGAATATCGTCCATATtg                                   >  6:223954/1‑149 (MQ=255)
                                                                                                                  acaGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACTTTAATATTATGGTTCGGCACGTTTTGCAGAATATCTTAGATATtgt                                  >  2:124061/1‑149 (MQ=255)
                                                                                                                   caGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACTTTAATATTATGGTTCGGCACGTTTTGCAGAATATCGTCCata                                      >  2:362534/1‑144 (MQ=255)
                                                                                                                   caGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACTTTAATATTATGGTTCGGCACGTTTTGCAGAATATCGTCCata                                      <  1:362534/144‑1 (MQ=255)
                                                                                                                   caGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACTTTAATATTATGGTTCGGCACGTTTTGCAGAATATCGTCCATATtgtg                                 <  8:121535/149‑1 (MQ=255)
                                                                                                                            taGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACTTTAATATTATGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTg                        <  8:347755/149‑1 (MQ=255)
                                                                                                                            taGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACTTTAATATTATGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTg                        >  8:249858/1‑149 (MQ=255)
                                                                                                                              gagCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACTTTAATATTATGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTgg                       >  7:60836/1‑148 (MQ=255)
                                                                                                                                  gACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACTTTAATATTATGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAaga                  <  7:249858/149‑1 (MQ=255)
                                                                                                                                   aCAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACTTTAATATTATCGTGCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAagag                 <  1:197038/149‑1 (MQ=255)
                                                                                                                                      gTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACTTTAATATTATGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATa              <  2:87208/149‑1 (MQ=255)
                                                                                                                                              aTCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACTTTAATATTATGGTTCGGCACGTTTTGCAGAATATCGt                                           <  1:66752/112‑1 (MQ=255)
                                                                                                                                              aTCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACTTTAATATTATGGTTCGGCACGTTTTGCAGAATATCGt                                           >  2:66752/1‑112 (MQ=255)
                                                                                                                                                  gAAGGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACTATAATATTATGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGt  <  6:368566/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GTCAGTGATACGCGGATTACGCCAGCCCATATACAGCGGATCGTTAAGCAGCTGTTGGTTGTTCGTTCCGACATCCAGCACCACCGGAAGGGTATACGCCGGGCTGATGCCGCCACAGGCGGTATAGAGCGACAGTTTACCGATCGGAATGCCCATCCCGCCGATGCCCTGGTCACCAAGCCCCAGAATGCGTTCACCGTCAGTCACCACAATCACTTTAATATTATGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGT  >  NC_000913/1555010‑1555304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: