Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,261,474	C→A	Q134H (CAG→CAT)	prs ←	phosphoribosylpyrophosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,261,474	0	C	A	100.0%	29.8 / NA	10	Q134H (CAG→CAT)	prs	phosphoribosylpyrophosphate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (5/5);  total (5/5)

GATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAG  >  NC_000913/1261336‑1261550                                                                                                                                           |                                                                             gATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGt                                                                    >  2:64642/1‑149 (MQ=255)  aTAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTg                                                                   >  2:31540/1‑149 (MQ=255)   tAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGc                                                                  <  1:31540/149‑1 (MQ=255)                 acaACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTg                                                                   >  3:80480/1‑134 (MQ=255)                 acaACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTg                                                                   <  4:80480/134‑1 (MQ=255)                                tCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCAGGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACAcc                                     <  8:58695/149‑1 (MQ=255)                                    gagaAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACg                                 >  4:71375/1‑149 (MQ=255)                                           cacaATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGgaga                          <  1:64642/149‑1 (MQ=255)                                                                                                                                  aCCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAg  >  5:86930/1‑86 (MQ=255)                                                                                                                                  aCCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAg  <  6:86930/86‑1 (MQ=255)                                                                                                                                           |                                                                             GATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAG  >  NC_000913/1261336‑1261550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑