BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,317,667	T→G	E737A (GAA→GCA)	rcsC ←	hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,317,667	0	T	G	100.0%	369.8 / NA	103	E737A (GAA→GCA)	rcsC	hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (55/48); total (55/48)

Only 100 of 104 total aligned reads displayed.

CCACACTGTGTACCCACTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTTCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCGGGTACTGAGCGCCGTACAACG  >  NC_000913/2317521‑2317811

  actcTGTGTACCCACTCCCCTGGCGCTTTCTCCAGCTGAATACCAATCTGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTGAGTGATCAACTCATCTTCGGTAGTCGGTTCCTGCCCTGCGTa                                                                                                                                              <  1:276901/146‑1 (MQ=255)

  acacTGTGTACCCACTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTa                                                                                                                                              >  2:204868/1‑149 (MQ=255)

    acTGTGTACCCACTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTAtg                                                                                                                                            >  8:288493/1‑149 (MQ=255)

            cccACTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAacg                                                                                                                                    >  4:90730/1‑149 (MQ=255)

             ccACTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAacga                                                                                                                                   >  8:186220/1‑149 (MQ=255)

             ccACTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAacga                                                                                                                                   >  2:217918/1‑149 (MQ=255)

             ccACTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAacga                                                                                                                                   <  8:369940/149‑1 (MQ=255)

              cACTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAacgac                                                                                                                                  <  3:131117/149‑1 (MQ=255)

               aCTCCCCTGGCGCTTTCTCCAGCGTAATACCAATATGGCGACGACAGCAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCACGTAATTGATCAACACATCTTCTGGAGACGGTTCCTGCAATGCGTAAGTTTTAacgac                                                                                                                                  >  5:497354/1‑148 (MQ=255)

                   cccTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCATCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTAtgtt                                                                                                                                          >  8:220660/1‑136 (MQ=255)

                   cccTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCATCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTAtgtt                                                                                                                                          <  7:220660/136‑1 (MQ=255)

                   cccTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACATCGTCAGTGATCAACACATCTGCGGGAGTCGGTTCATGCCCTGCGGATGTTGTCACGACGATGc                                                                                                                             >  3:398131/1‑149 (MQ=255)

                     cTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCcg                                                                                                                           >  3:225748/1‑149 (MQ=255)

                       ggCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCcgct                                                                                                                         >  7:33914/1‑149 (MQ=255)

                                tcCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGCTCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCa                                                                                                                 >  7:273216/1‑148 (MQ=255)

                                tcCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCa                                                                                                                 >  1:127463/1‑148 (MQ=255)

                                tcCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCa                                                                                                                 >  3:304660/1‑148 (MQ=255)

                                tcCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCa                                                                                                                 >  5:494531/1‑148 (MQ=255)

                                tcCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCa                                                                                                                 >  5:536299/1‑148 (MQ=255)

                                 ccAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCaaa                                                                                                               >  4:151483/1‑149 (MQ=255)

                                   aGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACt                                                                                                             >  7:378757/1‑149 (MQ=255)

                                          tACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTcc                                                                                                      <  3:151483/149‑1 (MQ=255)

                                            ccAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCa                                                                                                     <  8:303480/148‑1 (MQ=255)

                                            ccAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAg                                                                                                    <  7:566686/149‑1 (MQ=255)

                                            ccAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAg                                                                                                    <  6:245605/149‑1 (MQ=255)

                                             cAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAg                                                                                                    >  2:182174/1‑148 (MQ=255)

                                             cAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAg                                                                                                    >  4:244891/1‑148 (MQ=255)

                                               atatGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTAtgtt                                                                                                                                          >  2:68234/1‑108 (MQ=255)

                                               atatGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTAtgtt                                                                                                                                          <  1:68234/108‑1 (MQ=255)

                                               atatGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGaa                                                                                                 <  8:378757/149‑1 (MQ=255)

                                                  tGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACt                                                                                               <  4:304660/148‑1 (MQ=255)

                                                     cgacgaCAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACa                                                                                           <  1:28535/149‑1 (MQ=255)

                                                        cgacAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACagag                                                                                        <  1:8974/149‑1 (MQ=255)

                                                         gacAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGc                                                                                       >  1:222498/1‑149 (MQ=255)

                                                           cAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGa                                                                                     <  2:127463/149‑1 (MQ=255)

                                                           cAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGa                                                                                     >  4:332786/1‑149 (MQ=255)

                                                            aGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGAc                                                                                    <  1:206391/149‑1 (MQ=255)

                                                            aGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGAc                                                                                    >  4:124829/1‑149 (MQ=255)

                                                            aGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGAc                                                                                    <  8:33914/149‑1 (MQ=255)

                                                              aaGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACg                                                                                   >  7:344443/1‑148 (MQ=255)

                                                                 gTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCAtt                                                                               <  3:244891/149‑1 (MQ=255)

                                                                 gTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATATTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCAtt                                                                               >  6:358540/1‑149 (MQ=255)

                                                                   cACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGc                                                                             >  2:494594/1‑149 (MQ=255)

                                                                    aCTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCg                                                                            <  1:270171/149‑1 (MQ=255)

                                                                    aCTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCg                                                                            <  6:494531/149‑1 (MQ=255)

                                                                    aCTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCGGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCg                                                                            >  3:450629/1‑149 (MQ=255)

                                                                        ccGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAg                                                                                                    <  1:246915/121‑1 (MQ=255)

                                                                        ccGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAg                                                                                                    >  2:246915/1‑121 (MQ=255)

                                                                        ccGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGaa                                                                                                 >  2:21780/1‑124 (MQ=255)

                                                                        ccGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGaa                                                                                                 <  1:21780/124‑1 (MQ=255)

                                                                        ccGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGa                                                                          <  1:483297/147‑1 (MQ=255)

                                                                              tgccctgccATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCgcca                                                                    <  3:124829/147‑1 (MQ=255)

                                                                                ccctgccATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCgccagcc                                                                 <  1:204868/148‑1 (MQ=255)

                                                                                 cctgccATTTTTTACTCACTACCTCGTCAGTGATCAACACATGTTCGGGAGTCGGTTCCTGCCGTGCGTATGTTGTGACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGCCAGAGCGCCGCATTGCGGACCGccagccag                                                               >  2:219067/1‑149 (MQ=255)

                                                                                  ctgccATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGccagccag                                                               >  1:558163/1‑148 (MQ=255)

                                                                                   tgccATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACa                                                                                           >  5:166343/1‑119 (MQ=255)

                                                                                   tgccATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACa                                                                                           <  6:166343/119‑1 (MQ=255)

                                                                                   tgccATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGcccgccag                                                               >  6:344672/1‑147 (MQ=255)

                                                                                   tgccATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGccagccag                                                               >  6:217139/1‑147 (MQ=255)

                                                                                    gccATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTAtgttgt                                                                                                                                        <  6:235726/73‑1 (MQ=255)

                                                                                    gccATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTAtgttgt                                                                                                                                        >  5:235726/1‑73 (MQ=255)

                                                                                         tttttACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTACCGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGACCTGACAGAGCGCCGCATTGCGGCCCCCCAGCCAGCAGCGtt                                                        >  1:476462/1‑148 (MQ=255)

                                                                                          ttttACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGttt                                                       <  3:90730/148‑1 (MQ=255)

                                                                                            ttACTCACTACCTCGTCAGTGATCACCACATCTTCGGGAGTCGGTTCCTGCCCAGCGTATCTAGTAACGACGATGCCGCTGCGCTGCAAACTGGGTTCCAGGACCTGACAGAGCGACGCATTGCGGACCGCCCGCCAGCCGCGTATAc                                                     >  4:381484/1‑148 (MQ=255)

                                                                                                tCACTACCTCGTCAGTGATCAACACATCTTCGCGAGGCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGCCGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACt                                                 >  5:442275/1‑148 (MQ=255)

                                                                                                  aCTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCaa                                              >  5:500/1‑149 (MQ=255)

                                                                                                   cTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACt                                                                                               <  5:6748/99‑1 (MQ=255)

                                                                                                   cTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACt                                                                                               >  6:14404/1‑99 (MQ=255)

                                                                                                   cTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACt                                                                                               >  6:6748/1‑99 (MQ=255)

                                                                                                   cTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACt                                                                                               <  5:14404/99‑1 (MQ=255)

                                                                                                    tACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGaa                                                                                                 <  6:25621/96‑1 (MQ=255)

                                                                                                    tACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGaa                                                                                                 >  5:25621/1‑96 (MQ=255)

                                                                                                          gTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTc                                        <  5:217139/147‑1 (MQ=255)

                                                                                                          gTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTc                                        <  1:217918/147‑1 (MQ=255)

                                                                                                               tGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCt                                 <  1:182174/149‑1 (MQ=255)

                                                                                                                 aTCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCtt                                <  8:344443/148‑1 (MQ=255)

                                                                                                                  tCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCtttt                              <  7:115602/149‑1 (MQ=255)

                                                                                                                  tCAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCtttt                              <  4:225748/149‑1 (MQ=255)

                                                                                                                   cAACACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCttttt                             >  5:53894/1‑149 (MQ=255)

                                                                                                                    aaCACATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCATTCCACGCCttttt                             >  8:528400/1‑148 (MQ=255)

                                                                                                                     acacATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCAc                                                  <  7:456494/126‑1 (MQ=255)

                                                                                                                     acacATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCAc                                                  >  8:456494/1‑126 (MQ=255)

                                                                                                                     acacATCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTc                           <  5:358540/149‑1 (MQ=255)

                                                                                                                      cacaTCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCCCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCt                          >  5:271015/1‑149 (MQ=255)

                                                                                                                        caTCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGc                        <  7:288493/149‑1 (MQ=255)

                                                                                                                        caTCTTCGGGAGTAGTGTCCTTCCCTGATGATGTTGTAACGACGATGCCGATTCGCTGCAAACTGGTTTCCAGGACCTGACAGAGCTACGCATTGCTGACCGCCAGCCAGAAGCGTTTACCACTCAAACCTTCCACGCCTTTTTTCTGc                        <  4:398131/149‑1 (MQ=255)

                                                                                                                         aTCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCg                       >  7:357420/1‑149 (MQ=255)

                                                                                                                         aTCTTCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCg                       >  2:509263/1‑149 (MQ=255)

                                                                                                                            ttCGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCggg                     >  7:387086/1‑148 (MQ=255)

                                                                                                                              cGGGAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCgg                      <  3:441899/145‑1 (MQ=255)

                                                                                                                                 gAGTCGGTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCGGGTACt                 >  1:225840/1‑147 (MQ=255)

                                                                                                                                      ggTTCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCGGGTACTGAGCGcc          <  6:53894/149‑1 (MQ=255)

                                                                                                                                        ttCCTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCGGGTACTGAGCGCCGt        <  3:332786/149‑1 (MQ=255)

                                                                                                                                          cctgccctgcGTATGTTGGAACGACGATGCCGCTGCGCTGCAACCTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACGACTCACCCCTTCCACGCCTTTTTTCTGCGGGTACTGAGCGCCGTa       <  3:381484/148‑1 (MQ=255)

                                                                                                                                           cTGCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCGGGTACTGAGCGCCGTAc      >  8:431234/1‑148 (MQ=255)

                                                                                                                                             gCCCTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCGGGTACTGAGCGCCGTACAAc   >  5:220930/1‑149 (MQ=255)

                                                                                                                                              cccTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCGGGTACTGAGCGCCGTACAAc   <  1:509263/148‑1 (MQ=255)

                                                                                                                                              cccTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCGGGTACTGAGCGCCGTACAACg  <  8:357420/149‑1 (MQ=255)

                                                                                                                                               ccTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTcc                                       <  2:501431/111‑1 (MQ=255)

                                                                                                                                               ccTGCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTcc                                       >  1:501431/1‑111 (MQ=255)

CCACACTGTGTACCCACTCCCCTGGCGCTTTCTCCAGCGGAATACCAATATGGCGACGACAGAAGGTCACTACCGCTCTGCCCTGCCATTTTTTACTCACTACCTCGTCAGTGATCAACACATCTTCGGGAGTCGGTTCCTGCCCTTCGTATGTTGTAACGACGATGCCGCTGCGCTGCAAACTGGTTTCCAGGAACTGACAGAGCGACGCATTGCGGACCGCCAGCCAGCAGCGTTTACCACTCAACCCTTCCACGCCTTTTTTCTGCGGGTACTGAGCGCCGTACAACG  >  NC_000913/2317521‑2317811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`