Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,632,046	G→T	P164T (CCG→ACG)	guaA ←	GMP synthetase (glutamine aminotransferase)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,632,046	0	G	T	100.0%	328.0 / NA	93	P164T (CCG→ACG)	guaA	GMP synthetase (glutamine aminotransferase)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (48/45);  total (48/45)

CTGGCGTCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCTTCGATACCGCGAACCAGTGCGCTGTCGTTTAC  >  NC_000913/2631905‑2632190                                                                                                                                              |                                                                                                                                                 cTGGCGTCCACAGGGCTGCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCtt                                                                                                                                           <  3:14699/149‑1 (MQ=255)    gCGTCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCttt                                                                                                                                          <  7:113510/147‑1 (MQ=255)         cacaGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGc                                                                                                                                    <  4:76125/148‑1 (MQ=255)         caaaGGGCTTCACACTGGCAGCTATCACGCACTAAACGCTCCAGCATGCGCATACCCTGGCGGGTTTGAGTCACTTCCGGGGGGAACTGTAAGCCATAGAATCGTTTTTCTTCGTTTGCCATAATGGCAAACGTGCAGCTTTCGGTGCt                                                                                                                                   <  6:418131/146‑1 (MQ=255)          acaGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTg                                                                                                                                  <  8:249922/149‑1 (MQ=255)            aGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGc                                                                                                                                >  1:310625/1‑149 (MQ=255)            aGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGc                                                                                                                                >  4:477291/1‑149 (MQ=255)                 ttCCCACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACgg                                                                                                                           >  6:26650/1‑149 (MQ=255)                 ttCACACTGGCAGATATCACGCACAAAACGCTCCAGCCTGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCTGTGCTGGCTACgg                                                                                                                           >  2:363683/1‑149 (MQ=255)                 ttCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACgg                                                                                                                           >  7:545782/1‑149 (MQ=255)                 ttCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACgg                                                                                                                           >  4:127338/1‑149 (MQ=255)                   cacaCTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACgg                                                                                                                           <  1:267599/147‑1 (MQ=255)                      acTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCg                                                                                                                                        <  1:120131/131‑1 (MQ=255)                      acTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCg                                                                                                                                        >  2:120131/1‑131 (MQ=255)                      acTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGtgat                                                                                                                       >  3:105196/1‑148 (MQ=255)                        tGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGtgatg                                                                                                                      >  6:18373/1‑147 (MQ=255)                        tGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGaa                                                                                                                    >  6:10668/1‑149 (MQ=255)                          gCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGaa                                                                                                                    <  2:373162/147‑1 (MQ=255)                          gCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGaa                                                                                                                    <  5:313891/147‑1 (MQ=255)                          gCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAg                                                                                                                   <  2:339335/148‑1 (MQ=255)                          gCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGt                                                                                                                  <  3:477291/149‑1 (MQ=255)                            aGATATCACGCACAAAACGCTCCAGCATGCGCATACACTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTcg                                                                                                                >  4:49992/1‑149 (MQ=255)                                 tCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTcggacg                                                                                                            >  2:14913/1‑148 (MQ=255)                                   aCGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGaa                                                                                                         <  5:283639/149‑1 (MQ=255)                                        caAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCg                                                                                                    <  3:318137/149‑1 (MQ=255)                                        caAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGGCGGACGGAATAGCg                                                                                                    <  5:26650/149‑1 (MQ=255)                                         aaaaCGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCgg                                                                                                   >  7:451284/1‑149 (MQ=255)                                             cGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAAc                                                                                               <  8:545782/149‑1 (MQ=255)                                                  cAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTa                                                                                           <  1:265402/148‑1 (MQ=255)                                                   aGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATc                                                                                         <  8:504854/149‑1 (MQ=255)                                                        gcgcATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGt                                                                                    >  7:144624/1‑149 (MQ=255)                                                          gcATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTgg                                                                                  >  2:145893/1‑149 (MQ=255)                                                          gcATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTgg                                                                                  >  2:299105/1‑149 (MQ=255)                                                          gcATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTgg                                                                                  <  8:451284/149‑1 (MQ=255)                                                                ccTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGtgatg                                                                                                                      <  6:40325/107‑1 (MQ=255)                                                                ccTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGtgatg                                                                                                                      >  5:40325/1‑107 (MQ=255)                                                                ccTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGcc                                                                                      <  4:110439/139‑1 (MQ=255)                                                                ccTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGcc                                                                                      >  3:110439/1‑139 (MQ=255)                                                                 cTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATcc                                                                           <  5:209924/149‑1 (MQ=255)                                                                      gggTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCa                                                                          >  6:538735/1‑145 (MQ=255)                                                                      gggTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCa                                                                          <  5:538735/145‑1 (MQ=255)                                                                        gTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATc                                                                    <  5:10668/149‑1 (MQ=255)                                                                        gTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATc                                                                    <  5:18373/149‑1 (MQ=255)                                                                            gAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGag                                                                 >  3:336606/1‑148 (MQ=255)                                                                             aGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAAc                                                                                               <  6:393390/117‑1 (MQ=255)                                                                             aGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAAc                                                                                               >  5:393390/1‑117 (MQ=255)                                                                               tCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGagcagc                                                             >  2:41969/1‑149 (MQ=255)                                                                                 aCTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCgg                                                           >  3:288349/1‑149 (MQ=255)                                                                                  cTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGt                                                          <  3:127338/149‑1 (MQ=255)                                                                                   ttCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGtt                                                         >  3:56414/1‑149 (MQ=255)                                                                                     ccGGGTGGAACTGTACTCCATAGAAGCGTTTTTCTTAGTTAGCCATAATGGCAAACGTTCAGCTTTCGGTGCTGGCTACTTTTATGAAGTCGTACGGAATCGCGGGAACTGTATCGCCGGTGCTCATCCAGACATCGACAAGCCGttt                                                        >  5:388864/1‑148 (MQ=255)                                                                                      cGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACgg                                                                                                                           >  4:49562/1‑80 (MQ=255)                                                                                      cGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACgg                                                                                                                           <  3:49562/80‑1 (MQ=255)                                                                                       gggTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTAcc                                                     <  3:49992/149‑1 (MQ=255)                                                                                        ggTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCg                                                    >  8:314982/1‑149 (MQ=255)                                                                                         gTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCg                                                    >  5:432231/1‑148 (MQ=255)                                                                                          tGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTc                                                  >  7:522922/1‑149 (MQ=255)                                                                                            gAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTg                                                >  5:366127/1‑149 (MQ=255)                                                                                              aCTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGCCGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCg                                              <  3:127366/149‑1 (MQ=255)                                                                                               cTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCgg                                             >  3:481879/1‑149 (MQ=255)                                                                                                  tACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCa                                          <  6:250484/149‑1 (MQ=255)                                                                                                    cGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAgc                                        <  1:145893/149‑1 (MQ=255)                                                                                                    cGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCCCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAg                                         <  1:299105/148‑1 (MQ=255)                                                                                                     gCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAgcg                                       >  6:541454/1‑149 (MQ=255)                                                                                                       cATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCg                                                                                                    <  3:402923/86‑1 (MQ=255)                                                                                                       cATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCg                                                                                                    >  4:402923/1‑86 (MQ=255)                                                                                                       cATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGt                                                          <  3:454188/128‑1 (MQ=255)                                                                                                       cATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGt                                                          >  4:454188/1‑128 (MQ=255)                                                                                                       cATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCa                                     >  3:458488/1‑149 (MQ=255)                                                                                                       cATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCa                                     >  7:274324/1‑149 (MQ=255)                                                                                                       cATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCa                                     >  8:109260/1‑149 (MQ=255)                                                                                                       cATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCa                                     >  8:1443/1‑149 (MQ=255)                                                                                                       cATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCa                                     >  4:387237/1‑149 (MQ=255)                                                                                                        aTAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTc                                                                                                                 <  7:537020/72‑1 (MQ=255)                                                                                                        aTAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTc                                                                                                                 >  8:537020/1‑72 (MQ=255)                                                                                                            aaGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCTTc                                <  4:458488/149‑1 (MQ=255)                                                                                                                gTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGGGCATCTTCGAt                             >  1:395548/1‑148 (MQ=255)                                                                                                                gTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCTTCGAt                             <  2:395548/148‑1 (MQ=255)                                                                                                                gTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCTTCGATa                            >  4:269110/1‑149 (MQ=255)                                                                                                                   tttCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCTTCGATAcc                          <  6:366127/148‑1 (MQ=255)                                                                                                                     tcttcGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCTTCGATACcgc                        <  7:1443/148‑1 (MQ=255)                                                                                                                         cGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCTTCGATACCGCGa                      <  1:14913/146‑1 (MQ=255)                                                                                                                           ttAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCTTCGATACCGCGAACCAg                 >  6:50776/1‑149 (MQ=255)                                                                                                                           ttAGCAATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGAAGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCATCCATCGAGCAGCCGTTTACCGTCTGCGGTCCTCGCATCTTCGATACCCAGCACCAg                 >  6:22814/1‑149 (MQ=255)                                                                                                                             aGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCTTCGATACCGCGAACCAGt                <  4:481879/148‑1 (MQ=255)                                                                                                                             aGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCTTCGATACCGCGAACCAGt                <  4:336606/148‑1 (MQ=255)                                                                                                                              gCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCTTCGATACCGCGAACCAGTgc              >  7:80393/1‑149 (MQ=255)                                                                                                                               ccATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCTTCGATACCGCGAACCAGTgcg             >  5:403790/1‑149 (MQ=255)                                                                                                                                      ggCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCCGCGCATCTTAGATACCGCGAACCAGTGCGCTGTCGt      >  2:470279/1‑149 (MQ=255)                                                                                                                                        cAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCTTCGATACCGCGAACCAGTGCGCTGTCGttt    <  7:314982/149‑1 (MQ=255)                                                                                                                                         aaaCGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCTTCGATACCGCGAACCAGTGCGCTGTCGTTTa   >  5:447210/1‑149 (MQ=255)                                                                                                                                         aaaCGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCTTCGATACCGCGAACCAGTGCGCTGTCGTTTa   <  3:387237/149‑1 (MQ=255)                                                                                                                                          aaCGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCTTCGATACCGCGAACCAGTGCGCTGTCGTTTAc  <  8:245909/149‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                 CTGGCGTCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCTTCGATACCGCGAACCAGTGCGCTGTCGTTTAC  >  NC_000913/2631905‑2632190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑