Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,864,997 C→T 5.3% E214K (GAA→AAA)  yeaE ← aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,864,9970CT 5.3% 245.7 / 2.2 75E214K (GAA→AAA) yeaEaldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base C (34/37);  new base T (2/2);  total (36/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.36e-01

CTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCACCGGCATCTGCTGTTGCTGGCACCAGGGGAGTAGATCGTACTCAATTCCTCGTGAACCGAGATGGTAAAG  >  NC_000913/1864850‑1865138
                                                                                                                                                   |                                                                                                                                             
gtctagaaAGTTGGACCTCAAGCACTGCGGCATTTTGTTGGACATGCGCAATCGTGGCGGCTTTTCGAATCGCGATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATTTTGTGAGCATGTGCAATTTCg                                                                                                                                              <  4:187213/145‑1 (MQ=255)
  gaagaaAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCg                                                                                                                                              >  3:187213/1‑147 (MQ=255)
  gaagaaAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGtt                                                                                                                                            >  3:323484/1‑149 (MQ=255)
  gaagaaAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCAGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCAAGCCAAAAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGtt                                                                                                                                            >  6:25536/1‑149 (MQ=255)
   aagaaAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGtt                                                                                                                                            <  7:323803/148‑1 (MQ=255)
   aagaaAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGtt                                                                                                                                            <  6:124184/148‑1 (MQ=255)
   aagaaAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGGGCAATTTCGtt                                                                                                                                            >  7:360748/1‑148 (MQ=255)
          ttCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACcg                                                                                                                                    >  8:219968/1‑149 (MQ=255)
              aCCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGtt                                                                                                                                >  1:166130/1‑149 (MQ=255)
                cTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGtttt                                                                                                                              >  1:216958/1‑149 (MQ=255)
                cTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGtttt                                                                                                                              <  3:311910/149‑1 (MQ=255)
                 tCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGtt                                                                                                                                            >  6:30077/1‑134 (MQ=255)
                 tCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGtt                                                                                                                                            <  5:30077/134‑1 (MQ=255)
                   aatcacaGCAGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGtttttt                                                                                                                            <  3:20402/145‑1 (MQ=255)
                     gCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAAc                                                                                                                         <  5:179039/149‑1 (MQ=255)
                      cacaGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCTATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACa                                                                                                                        <  8:9495/149‑1 (MQ=255)
                      cacaGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGTGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACa                                                                                                                        <  8:310454/149‑1 (MQ=255)
                      cacaGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACa                                                                                                                        <  6:283299/149‑1 (MQ=255)
                       acaGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAg                                                                                                                       <  6:228014/149‑1 (MQ=255)
                        caGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGt                                                                                                                      >  7:265018/1‑149 (MQ=255)
                           ccGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCa                                                                                                                   >  5:142731/1‑149 (MQ=255)
                           ccGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACACACGCCAACAATACTTGTGCCGCTCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCa                                                                                                                   <  1:302769/149‑1 (MQ=255)
                                     ttgGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAAcc                                                                                                         >  7:378845/1‑149 (MQ=255)
                                     ttgGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAAcc                                                                                                         >  8:170438/1‑149 (MQ=255)
                                          cATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCg                                                                                                    <  2:216958/149‑1 (MQ=255)
                                              ggCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCt                                                                                                >  5:79521/1‑149 (MQ=255)
                                              ggCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCt                                                                                                <  5:106302/149‑1 (MQ=255)
                                               gCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTg                                                                                               <  1:47164/149‑1 (MQ=255)
                                                 aaTCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTggg                                                                                             <  2:166130/149‑1 (MQ=255)
                                                     gTGGCCGCTGTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTaa                                                                                         <  2:283927/149‑1 (MQ=255)
                                                          cGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGAc                                                                                    >  4:45237/1‑149 (MQ=255)
                                                           gCTTTTGGAATAGACATCAAACCCTGATGAATGATCTGCAACGCCATCATTACTTGTGCCTCGCTGATTTTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCTCCCGGCCTGGGCTAACGGACt                                                                                   <  8:360748/149‑1 (MQ=255)
                                                             ttttGGAATCGCCATCACCCCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCCTTGCGCAACCGCCCGGCCTGGGCTAACGGGCTg                                                                                  >  6:264488/1‑148 (MQ=255)
                                                             ttttGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTg                                                                                  >  1:72552/1‑148 (MQ=255)
                                                               ttGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGt                                                                                 <  8:265018/147‑1 (MQ=255)
                                                                tGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTaa                                                                               <  4:137226/148‑1 (MQ=255)
                                                                       gCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCa                                                                        <  7:219968/148‑1 (MQ=255)
                                                                        ccATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCAcc                                                                      >  4:305709/1‑149 (MQ=255)
                                                                         cATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCACCg                                                                     <  3:305709/149‑1 (MQ=255)
                                                                           tCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACt                                                                                   <  3:202418/133‑1 (MQ=255)
                                                                           tCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACt                                                                                   >  4:202418/1‑133 (MQ=255)
                                                                           tCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCACCgg                                                                    >  4:49085/1‑148 (MQ=255)
                                                                           tCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCACCgg                                                                    >  8:361016/1‑148 (MQ=255)
                                                                            cacaCCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCACCCGCCCGGCCTGGGCTAACGGACTGTACGCCATCCCCGGCa                                                                  >  5:316187/1‑149 (MQ=255)
                                                                            cacaCCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCACCGGCa                                                                  >  1:353114/1‑149 (MQ=255)
                                                                               acCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCACCGGCATct                                                               >  2:133533/1‑149 (MQ=255)
                                                                                 ccTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCACCGGCATcggc                                                             >  5:245617/1‑149 (MQ=255)
                                                                                    gatgaCTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCACCGGCATCTGCTGt                                                          >  1:326541/1‑149 (MQ=255)
                                                                                         cTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAAAATCGTTGACTACCGCGTTTTTTAACAGTCCATAGCGCAACCGACCGGCCTGTGCTAACGGACTGTAAGCCATCACCGGCATCTGCTGTTGCtc                                                     >  2:295265/1‑148 (MQ=255)
                                                                                          tGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCACCGGCATCTGCTGTTGCTgg                                                    >  6:30890/1‑149 (MQ=255)
                                                                                            aTCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCACCGGCATCTGCTGTTGCTgg                                                    <  2:353114/147‑1 (MQ=255)
                                                                                               aCCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCTTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCACCGGCATCTGCTGTTGCTGGCACCa                                               <  2:72552/149‑1 (MQ=255)
                                                                                                 ccACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACTGACTGTAAGCCATCACCGGCATCTGCTGTTGCTGGCACCAgg                                             <  1:295265/149‑1 (MQ=255)
                                                                                                 ccACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCACCGGCATCTGCTGTTGCTGGCACCAgg                                             >  2:321664/1‑149 (MQ=255)
                                                                                                    cGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCACCGGCATCTGCTGTTGCTGGCACCAGGGGa                                          <  8:378845/149‑1 (MQ=255)
                                                                                                           aaTACTTGCGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCCTTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCACCGGCATCTGCTGTTGCTGGCACCAGGGGAGTAGATc                                   <  2:356335/149‑1 (MQ=255)
                                                                                                               cTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCACCGGCATCTGCTGTTGCTGGCACCAGGGGAGTAGATCGTa                                <  4:323484/148‑1 (MQ=255)
                                                                                                               cTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCACCGGCATCTGCTGTTGCTGGCACCAGGGGAGTAGATCGTAc                               <  3:45237/149‑1 (MQ=255)
                                                                                                                            gATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCACCGGCATCTGCTGTTGCTGGCACCAGGGGAGTAGATCGTACTCAATTCCTCGt                   >  3:172519/1‑148 (MQ=255)
                                                                                                                                ttGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCACCGGCATCTGCTGTTGCTGGCACCAggg                                            <  7:16570/119‑1 (MQ=255)
                                                                                                                                ttGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCACCGGCATCTGCTGTTGCTGGCACCAggg                                            >  8:16570/1‑119 (MQ=255)
                                                                                                                                  gttaGCATGTGAAATTTCGTTTACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCACCGGCATCTGCTGTTGCGGGCACCAGGGGAGTAGATCGTACTCAATTCCTCGTGAACCg             <  6:245617/145‑1 (MQ=255)
                                                                                                                                  gtgAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCACCGGCATCTGCTGTTGCTGGCACCAGGGGAGTAGATCGTACTCAATTCCTCGTGAACCg             <  1:321664/148‑1 (MQ=255)
                                                                                                                                   tgAGCATGTGCAATTTTGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCAt                                                                          >  8:109232/1‑86 (MQ=255)
                                                                                                                                   tgAGCATGTGCAATTTTGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCAt                                                                          >  8:114634/1‑86 (MQ=255)
                                                                                                                                   tgAGCATGTGCAATTTTGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCAt                                                                          <  7:109232/86‑1 (MQ=255)
                                                                                                                                   tgAGCATGTGCAATTTTGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCAt                                                                          <  7:114634/86‑1 (MQ=255)
                                                                                                                                   tgAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCACCGGCATCTGCTGTTGCTGGCACCAGGGGAGTAGATCGTACt                              <  6:239565/130‑1 (MQ=255)
                                                                                                                                   tgAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCACCGGCATCTGCTGTTGCTGGCACCAGGGGAGTAGATCGTACt                              >  5:239565/1‑130 (MQ=255)
                                                                                                                                     aGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCACCGGCATCTGCTGTTGCTGGCACCAGGGGAGTAGATCGTACTCAATTCCTCGTGAACCGAGAt         <  7:170438/149‑1 (MQ=255)
                                                                                                                                         tgtgCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCACCGGCATCTGCTGTTGCTGGCACCAGGGGAGTAGATCGTa                                <  6:218273/122‑1 (MQ=255)
                                                                                                                                         tgtgCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCACCGGCATCTGCTGTTGCTGGCACCAGGGGAGTAGATCGTa                                >  5:218273/1‑122 (MQ=255)
                                                                                                                                          gtgCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCACCGGCATCTGCTGTTGCTGGCACCAGGGGAGTAGATCGTACTCAATTCCTCGTGAACCgaga          >  4:213977/1‑143 (MQ=255)
                                                                                                                                          gtgCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCACCGGCATCTGCTGTTGCTGGCACCAGGGGAGTAGATCGTACTCAATTCCTCGTGAACCgaga          <  3:213977/143‑1 (MQ=255)
                                                                                                                                             cAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCACCGGCATCTGCTGTTGCTGGCACCAGGGGAGTAGATCGTACTCAATTCCTCGTGAACCGAGATGGTAAAg  >  6:256582/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
CTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCCGGCCTGGGCTAACGGACTGTAAGCCATCACCGGCATCTGCTGTTGCTGGCACCAGGGGAGTAGATCGTACTCAATTCCTCGTGAACCGAGATGGTAAAG  >  NC_000913/1864850‑1865138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑