Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,806,700 G→A 100% A111T (GCG→ACG)  pfkB → 6‑phosphofructokinase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,806,7000GA94.1% 204.5 / 3.1 70A111T (GCG→ACG) pfkB6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base G (3/1);  new base A (32/31);  total (37/33)
Fisher's exact test for biased strand distribution p-value = 6.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTAACCCAACTGATTTCCGCTGCGCAAAAA  >  NC_000913/1806554‑1806843
                                                                                                                                                  |                                                                                                                                               
gTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGcacg                                                                                                                                               >  6:88128/1‑149 (MQ=255)
        aCCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTaaa                                                                                                                                       <  2:212534/149‑1 (MQ=255)
            gCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGaa                                                                                                                                   <  2:23633/149‑1 (MQ=255)
                    cTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGATCAGTATCGTTTTGTTATGAAAGGCACGGAATTAAATGAAGATGAGtt                                                                                                                           >  5:94404/1‑149 (MQ=255)
                    cTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGtt                                                                                                                           >  8:50596/1‑149 (MQ=255)
                    cTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGtt                                                                                                                           >  7:315314/1‑149 (MQ=255)
                       gTTTCACTGTTGGCGGATGAAAATGTCCACGTCGCTACTGTAGAAGCCAAAGTCTGGACCCGGCAGAATTTAAACGTACATGTGGAAGCTAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACTGCATTAAATGAAGATGAGTTTCg                                                                                                                        >  3:205027/1‑149 (MQ=255)
                         ttCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGc                                                                                                                       >  4:93937/1‑148 (MQ=255)
                         ttCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGc                                                                                                                       >  4:89317/1‑148 (MQ=255)
                           taCTGGTGGCGGATGAAAATGTCCCCGGCGCTCCTGTAGAAGCCAAAGACTGGACCCGGGAGAATTTACACGTACTTGTGGATGCAAGCGGTGAGCTGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCa                                                                                                                     <  1:88985/147‑1 (MQ=255)
                            aCTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAg                                                                                                                    <  1:251217/148‑1 (MQ=255)
                               gTTGGTGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGa                                                                                                                              <  7:340089/135‑1 (MQ=255)
                               gTTGGTGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGa                                                                                                                              >  8:340089/1‑135 (MQ=255)
                                ttGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCtt                                                                                                                 <  7:50596/147‑1 (MQ=255)
                                   gCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGCCTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTGTGTTATGCCAGGCCCGGCATTAAATGAAGATGAGTTGCGCCAGCTTGaa                                                                                                              >  4:274256/1‑147 (MQ=255)
                                        ttaaaaTGTCCCCGTCGGTACTGTATAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGGTAGCGGTTAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCaa                                                                                                        <  4:205027/146‑1 (MQ=255)
                                            aaTGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCt                                                                                                   <  5:88128/149‑1 (MQ=255)
                                                  cccGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGaaa                                                                                              <  1:238316/148‑1 (MQ=255)
                                                  cccGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAAt                                                                                             >  2:346978/1‑149 (MQ=255)
                                                  cccGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAAt                                                                                             >  4:167747/1‑149 (MQ=255)
                                                  cccGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAAt                                                                                             <  3:93937/149‑1 (MQ=255)
                                                  cccGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAAt                                                                                             <  3:89317/149‑1 (MQ=255)
                                                   ccGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAAtt                                                                                            <  8:1637/149‑1 (MQ=255)
                                                      tCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGaaa                                                                                              <  4:211916/144‑1 (MQ=255)
                                                      tCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGaaa                                                                                              >  3:211916/1‑144 (MQ=255)
                                                          tACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCg                                                                                     >  1:80687/1‑149 (MQ=255)
                                                            cTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCgg                                                                                    <  6:69481/148‑1 (MQ=255)
                                                               tAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGc                                                                                 >  3:220818/1‑148 (MQ=255)
                                                               tAGAAGCCAAAGACTGGACCCGGAAGAATTTACACGTACATGTGGAAGCAAGCGGGGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCgggg                                                                                  <  3:286658/147‑1 (MQ=255)
                                                                 gAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGaa                                                                                         >  1:298485/1‑138 (MQ=255)
                                                                 gAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGaa                                                                                         <  2:298485/138‑1 (MQ=255)
                                                                      cAAAGACTGGACCCGGCAGAATTTACACGTACTTGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTgg                                                                         <  7:189778/149‑1 (MQ=255)
                                                                      cAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTgg                                                                         >  3:142116/1‑149 (MQ=255)
                                                                      cAAAGACAGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTCAGCAGTAACGTTTTGTTATGCCAGGCACGGCAATAAATGAAGATGAATTTCGCCAGCTTGAACAGCAAGTTCTGGAAATTTAATCCGATGCCATCCTgg                                                                         >  8:189778/1‑149 (MQ=255)
                                                                       aaaGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGt                                                                        >  7:197579/1‑149 (MQ=255)
                                                                       aaaGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGt                                                                        >  1:280214/1‑149 (MQ=255)
                                                                        aaGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGt                                                                        >  6:271901/1‑148 (MQ=255)
                                                                        aaGACTGGAAACGGCAGAATTTACACGTACATGTGGAATAAAGCGGGGAGCAGTATCGTTTTGTTATTCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGCGCAAGTTCGGGAAATTGATTCCGGGGCCCTCCTGGt                                                                        >  2:61123/1‑148 (MQ=255)
                                                                             tGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAg                                                                  >  1:272726/1‑149 (MQ=255)
                                                                                 cccGGCAGAATTTACACGTACATGTGGCAGCGAGCGGTGCGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATCAGCGGa                                                              <  6:101774/149‑1 (MQ=255)
                                                                                  ccGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCgg                                                               >  1:217470/1‑147 (MQ=255)
                                                                                    ggCAGAATTTACACGTACATGTGGAAGCAAGAGGTGAGCAGTATAGTTTTGTTATGCCAGGCAAGGCATTAAATGAAGATGAGTTTCGCCAGCTCGAAGAGAAAGTTCTGGAAATTGAATCCGGGGACATCCTGGTCATAAGAGGAAGc                                                           >  1:91877/1‑149 (MQ=255)
                                                                                     gCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAg                                                            <  4:119278/147‑1 (MQ=255)
                                                                                     gCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAg                                                            >  3:119278/1‑147 (MQ=255)
                                                                                      cAGAATTTACAAGTACATGTGGAAGCAAGCGGTGATCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATCAGTTTCGCCAGCTTGAAGAGCAAGTTCGGGAAATTGAATCCGTGGCCATCCTGGTCATAAGCGGAAGc                                                           <  4:24681/147‑1 (MQ=255)
                                                                                              acacGTACATGTGGAAGCAACCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCAGCCTGGTCATAAGCGGAAGCCTGCCGCCa                                                  <  6:26551/148‑1 (MQ=255)
                                                                                                acGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGgt                                               >  3:167848/1‑149 (MQ=255)
                                                                                                      atttgGAAGCAAGCCGTGAGCAGTCTCGTTTCGTTACGCCAGCCCCGGCATTAAATGAAGATGAGTAGCGCCAGCTTGAAGAGCAAGTTCTGGAAATTCAATCCGGTGCCATCCTGGTCATAAGCGCAAGCCTGCCGCCAGGTGTGaac                                         <  8:318993/146‑2 (MQ=255)
                                                                                                      aTGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGCTGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGGCATAAGCGGAAGCCTGCCGCCAGGTGTGAAg                                         <  3:274256/149‑1 (MQ=255)
                                                                                                       tgtgGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGc                                        <  4:142116/149‑1 (MQ=255)
                                                                                                        gtgGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAg                                                                  >  4:33039/1‑122 (MQ=255)
                                                                                                        gtgGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAg                                                                  <  3:33039/122‑1 (MQ=255)
                                                                                                            aagcaagcGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAAACCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGa                                    >  4:279764/1‑148 (MQ=255)
                                                                                                               caagcGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGaaaaa                                <  5:62565/149‑1 (MQ=255)
                                                                                                                   cGGTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTaa                            <  8:315314/149‑1 (MQ=255)
                                                                                                                    ggTGATCAGTATCGTTTTGTTATGCCAGGCAAGGCATTAAATGAAGATGAGGTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCATGTGTGAAGCTGGAAAAATTAAc                           <  1:61123/149‑1 (MQ=255)
                                                                                                                    ggTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTAAc                           >  3:224050/1‑149 (MQ=255)
                                                                                                                     gTGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTAAcc                          >  5:24413/1‑149 (MQ=255)
                                                                                                                      tGAGCAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTAAcc                          >  1:189997/1‑148 (MQ=255)
                                                                                                                          cAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTAACCCAACt                     <  1:346978/149‑1 (MQ=255)
                                                                                                                          cAGTATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTAACCCAACt                     <  3:167747/149‑1 (MQ=255)
                                                                                                                             tATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTc                                                                                                    <  6:364264/67‑1 (MQ=255)
                                                                                                                             tATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTc                                                                                                    >  5:364264/1‑67 (MQ=255)
                                                                                                                             tATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTg                                                        >  7:100649/1‑111 (MQ=255)
                                                                                                                             tATCGTTTTGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTg                                                        <  8:100649/111‑1 (MQ=255)
                                                                                                                                 gTTTTGTTATGCCAGGCCCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTAACCCAACTGATTTcc              >  4:305134/1‑149 (MQ=255)
                                                                                                                                  ttttGTTATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTAACCCAACTGATTTCCg             >  2:182368/1‑149 (MQ=255)
                                                                                                                                       ttATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTaa                            >  7:383028/1‑129 (MQ=255)
                                                                                                                                       ttATGCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTaa                            <  8:383028/129‑1 (MQ=255)
                                                                                                                                           gCCAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTAACCCAACTGATTTCCGCTGCGCaa     >  6:94630/1‑148 (MQ=255)
                                                                                                                                             cAGGCACGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTAACCCAACTGATTTCCGCTGCGCaaaaa  >  5:6769/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGTTATGCCAGGCGCGGCATTAAATGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGGGCCATCCTGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTAACCCAACTGATTTCCGCTGCGCAAAAA  >  NC_000913/1806554‑1806843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑