Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 911,690 A→G 6.0% V154A (GTT→GCT)  hcr ← HCP oxidoreductase, NADH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913911,6900AG 6.0% 205.7 / 2.1 66V154A (GTT→GCT) hcrHCP oxidoreductase, NADH‑dependent
Reads supporting (aligned to +/- strand):  ref base A (26/36);  new base G (2/2);  total (28/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGGCCGCAGGTCATCACGGTACGTGAAGCTAAGTCAGGTACACCTGCCAGCAGTTCGCGAGTGAGACGACCAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACGCATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCG  >  NC_000913/911542‑911833
                                                                                                                                                    |                                                                                                                                               
cGGGCCGCAGGTGATCACGGTACGTGAAGCTAACTCAGGTACACCTGCCAGCAGTTCGCGAGTGAGACGACCAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATaa                                                                                                                                                 <  1:351213/149‑1 (MQ=255)
 gggCCGCAGGTCATCACGGTACGTGAAGCTAAGTCAGGTACACCTGCCAGCAGTTCGCGAGTGAGACGACCAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAAc                                                                                                                                                <  8:278967/149‑1 (MQ=255)
 gggCCGCAGGTCATCACGGTACGTGAAGCTAAGTCAGGTACACCTGCCAGCAGTTCGCGAGTGAGACGACCAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATAGGCGAAAATAAc                                                                                                                                                <  2:48002/149‑1 (MQ=255)
     cGCAGGTCATCACGGTACGTGAAGCTAAGTCAGGTACACCTGCCAGCAGTTCGCGAGTGAGACGACCAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACG‑CACTCATCGGCGAAAATAACATCCt                                                                                                                                           <  6:61886/149‑1 (MQ=255)
      gCAGGTCATCACGGTACGTGAAGCTAAGTCAGGTACACCTGCCAGCAGTTCGCGAGTGAGACGACCAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCt                                                                                                                                           <  6:11154/149‑1 (MQ=255)
             atcaCGGTACGTGAAGCTAAGTCAGGTACACCTGCCAGCAGTTCGCGAGTGAGACGACCAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAGCATCCTgcggcg                                                                                                                                     >  1:195486/1‑148 (MQ=255)
             atcaCGGTACGTGAAGCTAAGTCAGGTACACCTGCCAGCAGTTCGCGAGTGAGACGACCAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAGCATCCTgcggcg                                                                                                                                     >  1:201315/1‑148 (MQ=255)
              tcaCGGTACGTGAAGCTAAGTCAGGTACACCTGCCAGCAGTTCGCGAGTGAGACGACCAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTa                                                                                                                                   <  8:25257/149‑1 (MQ=255)
                  ggTACGTGAAGCTAAGTCAGGTACACCTGCCAGCAGTTCGCGAGTGAGACGACCAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCa                                                                                                                               <  1:219320/149‑1 (MQ=255)
                        tGAAGCTAAGTCAGGTACACCTGCCAGCAGTTCGCGAGTGAGACGACCAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTg                                                                                                                          >  6:8465/1‑148 (MQ=255)
                        tGAAGCTAAGTCAGGTACACCTGCCAGCAGTTCGCGAGTGAGACGACCAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGt                                                                                                                         <  5:8465/149‑1 (MQ=255)
                        tGAAGCTAAGTCAGGTACACCTGCCAGCAGTTCGCGAGTGAGACGACCAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGt                                                                                                                         <  3:162507/149‑1 (MQ=255)
                         gAAGCTAAGTCAGGTACACCTGCCAGCAGTTCGCGAGTGAGACGACCAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTa                                                                                                                        <  1:1852/149‑1 (MQ=255)
                         gAAGCTAAGTCAGGTACACCTGCCAGCAGTTCGCGAGTGAGACGACCAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTa                                                                                                                        <  3:54257/149‑1 (MQ=255)
                           aGCTAAGTCAGGTACACCTGCCAGCAGTTCGCGAGTGAGACGACCAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGa                                                                                                                      <  8:366835/149‑1 (MQ=255)
                            gCTAAGTCAGGTACACCTGCCAGCAGTTCGCGAGTGAGACGACCAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGAt                                                                                                                     <  7:296541/149‑1 (MQ=255)
                             cTAAGTCAGGTACACCTGCCAGCAGTTCGCGAGTGAGACGACCAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGAt                                                                                                                     >  3:172621/1‑148 (MQ=255)
                                aGTCAGGTACACCTGCCAGCAGTTCGCGAGTGAGACGACCAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCCCCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCAcc                                                                                                                 <  4:160391/149‑1 (MQ=255)
                                  tCAGGTACACCTGCCAGCAGTTCGCGAGTGAGACGACCAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCg                                                                                                               >  7:314060/1‑149 (MQ=255)
                                      gTACACCTGCCAGCAGTTCGCGAGTGAGACGACCAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGcaca                                                                                                           >  5:299876/1‑149 (MQ=255)
                                                  gcagTTCGCGAGTGAGACGACCAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGa                                                                                               <  7:82107/149‑1 (MQ=255)
                                                     gTTCGCGAGTGAGACGACCAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACgg                                                                                            <  6:270628/149‑1 (MQ=255)
                                                           gAGTGAGACGACCAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGttcttc                                                                                      >  5:303068/1‑149 (MQ=255)
                                                           gAGTGAGACGACCAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGttcttc                                                                                      <  2:50229/149‑1 (MQ=255)
                                                           gAGTGAGACGACCAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGttcttc                                                                                      <  3:151116/149‑1 (MQ=255)
                                                             gTGAGACGACCAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCg                                                                                     >  8:187241/1‑148 (MQ=255)
                                                             gTGAGACGACCAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGc                                                                                    <  4:172621/149‑1 (MQ=255)
                                                               gagaCGACCAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAGCATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCaa                                                                                  <  2:201315/149‑1 (MQ=255)
                                                                agaCGACCAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAGCATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCaa                                                                                  <  2:195486/148‑1 (MQ=255)
                                                                  acgacCAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCGTCTCATCGGCGAAAATAACATCCTGCGGCGTACGCTCGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGcc                                                                               <  2:347271/149‑1 (MQ=255)
                                                                   cgacCAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCa                                                                              <  3:188857/149‑1 (MQ=255)
                                                                    gacCAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAg                                                                             >  1:35926/1‑149 (MQ=255)
                                                                     acCAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGc                                                                            <  7:187241/149‑1 (MQ=255)
                                                                       cAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAg                                                                                                                       <  3:199945/104‑1 (MQ=255)
                                                                       cAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAg                                                                                                                       >  4:199945/1‑104 (MQ=255)
                                                                       cAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGa                                                                          <  6:299876/149‑1 (MQ=255)
                                                                       cAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGa                                                                          >  6:82515/1‑149 (MQ=255)
                                                                        aGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGAc                                                                         >  4:40579/1‑149 (MQ=255)
                                                                        aGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGAc                                                                         >  7:289833/1‑149 (MQ=255)
                                                                                aGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTTCCGGTTAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGAGCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACGCATCGAc                                                                 <  8:144742/149‑1 (MQ=255)
                                                                                 gCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACGCATCGACa                                                                >  3:45093/1‑149 (MQ=255)
                                                                                    ttCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCCCTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATAACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCTCCGCATCGACATaa                                                             >  2:290118/1‑149 (MQ=255)
                                                                                    ttCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACGCATCGACATaa                                                             >  1:375742/1‑149 (MQ=255)
                                                                                       ggTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACGCATCGACATAATCg                                                          <  5:154510/149‑1 (MQ=255)
                                                                                         tAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACGTCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACGCATCGACATAATCGGc                                                        >  6:157038/1‑149 (MQ=255)
                                                                                            cGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACGCATCGACATAATCGGCGt                                                      >  4:12898/1‑148 (MQ=255)
                                                                                            cGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACGCATCGACATAATCGGCGt                                                      >  6:52178/1‑148 (MQ=255)
                                                                                             gTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACGCATCGACATAATCGGc                                                        <  1:359757/145‑1 (MQ=255)
                                                                                             gTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACGCATCGACATAATCGGc                                                        >  2:359757/1‑145 (MQ=255)
                                                                                              ttATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACGCATCGACATAATCGGCGTGAc                                                   >  8:286165/1‑149 (MQ=255)
                                                                                               tATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACGCATCGACATAATCGGCGTGAcg                                                  >  5:306844/1‑149 (MQ=255)
                                                                                                           ccaGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCTCCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACGCATCGACATAATCGGCGTGAcgccgc                                              >  1:324225/1‑141 (MQ=255)
                                                                                                           ccaGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACGCATCGACATAATCGGCGTGAcgccgc                                              <  2:324225/141‑1 (MQ=255)
                                                                                                                gTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACGCATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAg                                 <  7:217119/149‑1 (MQ=255)
                                                                                                                        aTAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACGCATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAgg                          >  2:108695/1‑148 (MQ=255)
                                                                                                                               cGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACGCATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTAt                   <  7:286165/148‑1 (MQ=255)
                                                                                                                               cGCCACTAATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACGCATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTAt                   <  5:80583/148‑1 (MQ=255)
                                                                                                                                gCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGTGACGCATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGc                                >  6:38074/1‑134 (MQ=255)
                                                                                                                                       aTCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACGCATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGc            <  7:293537/147‑1 (MQ=255)
                                                                                                                                       aTCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACGCATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCtt          >  5:360972/1‑149 (MQ=255)
                                                                                                                                        tCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACGCATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCttt         <  2:141764/149‑1 (MQ=255)
                                                                                                                                          ggCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACGCATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTa        <  4:45093/148‑1 (MQ=255)
                                                                                                                                          ggCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACGCATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTa        <  3:12898/148‑1 (MQ=255)
                                                                                                                                          ggCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACGCATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTAt       <  2:375742/149‑1 (MQ=255)
                                                                                                                                           gCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACGCATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTAtc      >  1:55914/1‑149 (MQ=255)
                                                                                                                                              aaaaTAACATCCTGCGGAGTACGCACGTTGTAGAAAACCCGCCCATCGGCCTGTGGCCCGTACTTCGCAAGCCACCGACGCATCGACATAATCGGCGTGCCGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTAtcgtc   >  6:166705/1‑149 (MQ=255)
                                                                                                                                               aaaTAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGATCTTCGCAAGCCAGCGACGCATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTAtcgtcg  <  5:82515/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
CGGGCCGCAGGTCATCACGGTACGTGAAGCTAAGTCAGGTACACCTGCCAGCAGTTCGCGAGTGAGACGACCAGCGATAAAGCCTTCGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACGCATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCG  >  NC_000913/911542‑911833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑