BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,807,043	T→A	L225Q (CTG→CAG)	pfkB →	6‑phosphofructokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,807,043	0	T	A	100.0%	376.9 / NA	105	L225Q (CTG→CAG)	pfkB	6‑phosphofructokinase II
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (52/53); total (52/53)

Only 100 of 105 total aligned reads displayed.

GCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTC  >  NC_000913/1806898‑1807183

gCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAggg                                                                                                                                           >  5:257624/1‑149 (MQ=255)

gCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAggg                                                                                                                                           >  2:325063/1‑149 (MQ=255)

gCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAggg                                                                                                                                           <  1:348072/149‑1 (MQ=255)

 cAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGt                                                                                                                                          <  8:357662/149‑1 (MQ=255)

 cAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGt                                                                                                                                          <  4:316636/149‑1 (MQ=255)

  aaTTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTc                                                                                                                                         <  6:294807/149‑1 (MQ=255)

   aTTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGGCGTTTCCCAGGGTcc                                                                                                                                        >  6:302878/1‑149 (MQ=255)

    ttGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCca                                                                                                                                       >  4:314705/1‑149 (MQ=255)

      ggTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCcac                                                                                                                                      >  5:79779/1‑148 (MQ=255)

       gTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACaa                                                                                                                                    <  3:314705/149‑1 (MQ=255)

           cATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAg                                                                                                                                >  2:434969/1‑149 (MQ=255)

            aTCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGTCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAgc                                                                                                                               >  8:216805/1‑149 (MQ=255)

            aTCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAgc                                                                                                                               >  1:50869/1‑149 (MQ=255)

            aTCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGAACATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAgc                                                                                                                               >  2:211757/1‑149 (MQ=255)

             tCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAgcg                                                                                                                              >  5:77257/1‑149 (MQ=255)

                 gTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTgg                                                                                                                          <  7:74495/149‑1 (MQ=255)

                      gtacgCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCTAACGCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGGCCACAAGGAGCGCTGGgtgtg                                                                                                                     <  5:287124/145‑2 (MQ=255)

                         aGCCTAACCAAAAAGAACTCAGTCCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTAAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGa                                                                                                                   <  2:238368/148‑1 (MQ=255)

                          gCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATa                                                                                                                 <  6:257624/149‑1 (MQ=255)

                            cTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGt                                                                                                               >  8:78452/1‑149 (MQ=255)

                            cTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGt                                                                                                               >  2:280902/1‑149 (MQ=255)

                             tAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGt                                                                                                               >  1:46423/1‑148 (MQ=255)

                                ccAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGaa                                                                                                            >  3:121638/1‑148 (MQ=255)

                                 cAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCCCCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCTTTAATAGCGCCAAGGCCACACTGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGCGTGTTGATAGTGaaa                                                                                                           >  4:252182/1‑148 (MQ=255)

                                 cAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGaaaa                                                                                                          >  3:53505/1‑149 (MQ=255)

                                  aaaaaGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAAc                                                                                                         >  6:54011/1‑149 (MQ=255)

                                      aGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTa                                                                                                     <  1:325063/149‑1 (MQ=255)

                                        aaCTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTAtt                                                                                                   <  5:54011/149‑1 (MQ=255)

                                        aaCTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTAtt                                                                                                   <  1:183744/149‑1 (MQ=255)

                                             aGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAggt                                                                                              <  2:150075/149‑1 (MQ=255)

                                             aGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAggt                                                                                              <  5:136435/149‑1 (MQ=255)

                                              gTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAAGCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAggtg                                                                                             <  8:434830/149‑1 (MQ=255)

                                                 tgcTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAggtggt                                                                                           <  8:383844/147‑1 (MQ=255)

                                                  gcTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGc                                                                                         >  1:244168/1‑149 (MQ=255)

                                                    tGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAgcgc                                                                                                                             <  1:353663/111‑1 (MQ=255)

                                                    tGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAgcgc                                                                                                                             >  2:353663/1‑111 (MQ=255)

                                                         aaTCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCaccacc                                                                                  >  1:5665/1‑149 (MQ=255)

                                                             gcgAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGt                                                                               >  3:191901/1‑148 (MQ=255)

                                                             gcgAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGt                                                                               <  1:280902/148‑1 (MQ=255)

                                                              cgAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTg                                                                              >  1:55666/1‑148 (MQ=255)

                                                               gAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGa                                                                             >  5:40051/1‑148 (MQ=255)

                                                               gAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACGGTATTCAGGTGGTGCCACCACCGGTGa                                                                             >  5:45918/1‑148 (MQ=255)

                                                                 aCTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGaaaa                                                                          <  1:434969/149‑1 (MQ=255)

                                                                 aCTAACCCAGCCGGACGATGTCCGCAAAGCCGAGCAGGAAATCGATAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTTTGTGTTTATAGTGAAAACTGTATTCAGGTGGTGCCAACACCGGTGaaaa                                                                          <  3:252182/149‑1 (MQ=255)

                                                                     aCCCAGTCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGcc                                                                       <  7:216805/148‑1 (MQ=255)

                                                                     aCCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCa                                                                      <  6:45738/149‑1 (MQ=255)

                                                                       ccAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGGTGTCGTTTCCCAGGGTCCACAAGGCGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCaga                                                                    <  6:79779/149‑1 (MQ=255)

                                                                         aGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGaaaa                                                                                                          >  8:168516/1‑109 (MQ=255)

                                                                         aGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCagag                                                                   >  3:112791/1‑148 (MQ=255)

                                                                             ggACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCg                                                              <  2:50869/149‑1 (MQ=255)

                                                                              gACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGt                                                             <  5:302878/149‑1 (MQ=255)

                                                                                   tGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGtt                                                            >  4:336339/1‑145 (MQ=255)

                                                                                   tGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGtt                                                            <  3:336339/145‑1 (MQ=255)

                                                                                    gTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGa                                                                                                                                 >  5:287811/1‑75 (MQ=255)

                                                                                    gTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGa                                                                                                                                 <  6:287811/75‑1 (MQ=255)

                                                                                     tCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCt                                                      >  5:425639/1‑149 (MQ=255)

                                                                                     tCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCt                                                      >  5:67655/1‑149 (MQ=255)

                                                                                       cGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAATGAGCGCTGGTTGTTGATAGTGAAAAc                                                                                                         <  2:118201/96‑1 (MQ=255)

                                                                                       cGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTgg                                                    >  3:318569/1‑149 (MQ=255)

                                                                                       cGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTg                                                     <  7:78452/148‑1 (MQ=255)

                                                                                       cGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTg                                                     <  4:191901/148‑1 (MQ=255)

                                                                                        gCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCGAAACGGGTTGTCGGTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTTATAGTAAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGt                                                   <  8:188131/149‑1 (MQ=255)

                                                                                        gCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGt                                                   <  7:82625/149‑1 (MQ=255)

                                                                                        gCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGt                                                   <  6:77257/149‑1 (MQ=255)

                                                                                          taaGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGa                                                 >  5:289552/2‑149 (MQ=255)

                                                                                          aaaGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGa                                                 >  5:84897/1‑149 (MQ=255)

                                                                                            aGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTg                                                           <  4:257800/137‑1 (MQ=255)

                                                                                            aGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTg                                                           >  3:257800/1‑137 (MQ=255)

                                                                                            aGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACa                                               <  6:425639/149‑1 (MQ=255)

                                                                                              ccGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCag                                                                     <  4:173897/125‑1 (MQ=255)

                                                                                              ccGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCag                                                                     >  3:173897/1‑125 (MQ=255)

                                                                                                gcgcAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCAt                                           <  3:110356/149‑1 (MQ=255)

                                                                                                 cgcAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATg                                          <  1:211757/149‑1 (MQ=255)

                                                                                                      gAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCgg                                     >  6:145827/1‑149 (MQ=255)

                                                                                                        aaTCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAggtggt                                                                                           <  1:175135/93‑1 (MQ=255)

                                                                                                        aaTCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAggtggt                                                                                           >  2:175135/1‑93 (MQ=255)

                                                                                                            gTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGTTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGAt                               <  4:53505/149‑1 (MQ=255)

                                                                                                            gTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTg                                                           <  3:296983/121‑1 (MQ=255)

                                                                                                            gTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTg                                                           >  4:296983/1‑121 (MQ=255)

                                                                                                            gTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGAt                               >  8:387667/1‑149 (MQ=255)

                                                                                                                aTAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGaca                           >  1:215805/1‑149 (MQ=255)

                                                                                                                   gCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACt                         >  3:204457/1‑148 (MQ=255)

                                                                                                                     ggCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGa                       >  4:220092/1‑148 (MQ=255)

                                                                                                                      gCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACa                                               >  6:149014/1‑123 (MQ=255)

                                                                                                                      gCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGaaa                     <  4:353309/149‑1 (MQ=255)

                                                                                                                        aaGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACt                   <  2:5665/149‑1 (MQ=255)

                                                                                                                          ggCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTgg                 <  4:204457/149‑1 (MQ=255)

                                                                                                                            ccAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGacac                          >  7:238310/1‑138 (MQ=255)

                                                                                                                            ccAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGacac                          <  8:238310/138‑1 (MQ=255)

                                                                                                                            ccAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTgg                 >  3:4973/1‑147 (MQ=255)

                                                                                                                            ccAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCa               <  2:55666/149‑1 (MQ=255)

                                                                                                                              aaaCGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGc                                             >  3:99638/1‑117 (MQ=255)

                                                                                                                              aaaCGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGc                                             <  4:99638/117‑1 (MQ=255)

                                                                                                                              aaaCGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAg              >  2:339686/1‑148 (MQ=255)

                                                                                                                              aaaCGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAg              >  6:272177/1‑148 (MQ=255)

                                                                                                                              aaaCGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACAACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGCCACTGAAACTGGCAg              >  3:9990/1‑148 (MQ=255)

                                                                                                                                  gggTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAAt         >  4:219966/1‑149 (MQ=255)

                                                                                                                                    gTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGc       <  7:387667/149‑1 (MQ=255)

                                                                                                                                    gTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGc       <  2:154191/149‑1 (MQ=255)

                                                                                                                                         cGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCctctc  <  6:289552/149‑1 (MQ=255)

GCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTC  >  NC_000913/1806898‑1807183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`