Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,662,350 C→T P599S (CCC→TCC)  ynfF → S‑ and N‑oxide reductase, A subunit, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,662,3500CT98.8% 285.4 / NA 83P599S (CCC→TCC) ynfFS‑ and N‑oxide reductase, A subunit, periplasmic
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (45/37);  minor base G (0/1);  total (45/38)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAACGCAAACCCATCTACTGGATGCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTC  >  NC_000913/1662205‑1662489
                                                                                                                                                 |                                                                                                                                           
tGAACGCAAACCCATCTACTGGATGCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGtcc                                                                                                                                           <  2:431235/148‑1 (MQ=255)
  aaCGCAAACCCATCTACTGGATGCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGAc                                                                                                                                        <  8:87870/149‑1 (MQ=255)
           ccATCTACTGGATGCTGAGTGAAGTCGCCAAACGCTTAGGACCTGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCATCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGATCGTAACCCTGAGATGTCCGTCTACgaggag                                                                                                                               <  2:103681/149‑1 (MQ=255)
             aTCTACTGGATGCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGAt                                                                                                                             >  7:392046/1‑149 (MQ=255)
                     gATGCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGa                                                                                                                     >  5:113469/1‑149 (MQ=255)
                     gATGCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGa                                                                                                                     >  7:377712/1‑149 (MQ=255)
                      aTGCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGAc                                                                                                                    <  8:377712/149‑1 (MQ=255)
                         cTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCg                                                                                                                  <  2:237143/148‑1 (MQ=255)
                                aaGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCttt                                                                                                          <  3:312913/149‑1 (MQ=255)
                                 aGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCttt                                                                                                          >  1:87594/1‑148 (MQ=255)
                                       cAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGATAACGACCGGGATCTTTAAGaaaa                                                                                                   <  6:361939/149‑1 (MQ=255)
                                        aaaCGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCgg                                                                                                                 >  6:112544/1‑134 (MQ=255)
                                        aaaCGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCgg                                                                                                                 <  5:112544/134‑1 (MQ=255)
                                         aaCGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAACACGACCGGGATCTTTAAGaaaaaa                                                                                                 >  4:323442/1‑149 (MQ=255)
                                            gCTTAGGACAAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGc                                                                                              >  1:59015/1‑149 (MQ=255)
                                                aGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGaaa                                                                                                    >  2:121540/1‑139 (MQ=255)
                                                aGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGaaa                                                                                                    <  1:121540/139‑1 (MQ=255)
                                                  gACCATACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGGCCGACTACGAGGAGATGAAAACGCCCGGGATCTTTAAGAAAAAATGCCCGgaa                                                                                        <  2:347353/149‑1 (MQ=255)
                                                  gACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCgg                                                                                          >  6:311913/1‑147 (MQ=255)
                                                  gACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGgaa                                                                                        <  1:259453/149‑1 (MQ=255)
                                                     cAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGgaagaa                                                                                     >  5:273274/1‑149 (MQ=255)
                                                     cAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGgaagaa                                                                                     <  8:392046/149‑1 (MQ=255)
                                                               tCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTAc                                                                               <  5:57582/145‑1 (MQ=255)
                                                               tCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTAc                                                                               >  6:57582/1‑145 (MQ=255)
                                                                cAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGc                                                                          >  5:151745/1‑149 (MQ=255)
                                                                 aaaCCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTaa                                                                                                        <  5:47713/118‑1 (MQ=255)
                                                                 aaaCCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTaa                                                                                                        >  6:47713/1‑118 (MQ=255)
                                                                 aaaCCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCt                                                                         >  2:202329/1‑149 (MQ=255)
                                                                  aaCCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCt                                                                                                            <  2:77972/113‑1 (MQ=255)
                                                                  aaCCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCt                                                                                                            >  1:77972/1‑113 (MQ=255)
                                                                  aaCCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAacac                                                                                  <  3:230173/139‑1 (MQ=255)
                                                                  aaCCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAacac                                                                                  >  4:230173/1‑139 (MQ=255)
                                                                        tACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCcgc                                                                   <  6:113469/148‑1 (MQ=255)
                                                                          cTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCgcgc                                                                 >  6:220023/1‑148 (MQ=255)
                                                                          cTGAAGCTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTACCCCTGAGATGTCCGACTACGAGGAGAAGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCa                                                                >  6:279093/1‑149 (MQ=255)
                                                                           tGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGaaaaaa                                                                                                 >  7:44290/1‑115 (MQ=255)
                                                                            gAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCAtt                                                              >  2:53547/1‑149 (MQ=255)
                                                                            gAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCAtt                                                              >  2:412947/1‑149 (MQ=255)
                                                                             aaGGTCGCAGTCAGCATGAATGGCTCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTc                                                             <  4:48013/149‑1 (MQ=255)
                                                                              aGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCgg                                                                                          >  4:357658/1‑119 (MQ=255)
                                                                              aGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCgg                                                                                          <  3:357658/119‑1 (MQ=255)
                                                                               ggTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCg                                                           >  6:389645/1‑149 (MQ=255)
                                                                                 tCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGgaa                                                                                        >  6:377067/1‑118 (MQ=255)
                                                                                 tCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGgaa                                                                                        <  6:346951/118‑1 (MQ=255)
                                                                                 tCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGgaa                                                                                        <  5:377067/118‑1 (MQ=255)
                                                                                   gCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGaa                                                       <  3:58005/149‑1 (MQ=255)
                                                                                       tCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTa                                                                                                         <  1:278340/95‑1 (MQ=255)
                                                                                       tCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTa                                                                                                         >  2:278340/1‑95 (MQ=255)
                                                                                        cAGCATGAATGGATCTATTATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATc                                                   <  8:362261/148‑1 (MQ=255)
                                                                                           cATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCca                                                 >  5:56683/1‑147 (MQ=255)
                                                                                           cATGAATGGATAAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCcac                                                <  6:273274/148‑1 (MQ=255)
                                                                                              gAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAgg                                             >  1:360442/1‑148 (MQ=255)
                                                                                               aaTGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGc                                            >  5:25590/1‑148 (MQ=255)
                                                                                                     tCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCg                                     >  7:68806/1‑149 (MQ=255)
                                                                                                      cAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGaa                                                       >  7:151048/1‑130 (MQ=255)
                                                                                                      cAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGaa                                                       <  8:151048/130‑1 (MQ=255)
                                                                                                       aaaTATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGtt                                   <  1:202329/149‑1 (MQ=255)
                                                                                                        aaTATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCTCGCATTCCGTGAAGATCCACAGGCAAACCCGtt                                   >  2:307724/1‑148 (MQ=255)
                                                                                                         atatCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGAGCATTCCGTGCAGATCCACAGGCAACCCCGTTg                                  >  2:128435/1‑148 (MQ=255)
                                                                                                             ctcCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGa                                                     <  8:312141/125‑1 (MQ=255)
                                                                                                             ctcCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGa                                                     >  7:312141/1‑125 (MQ=255)
                                                                                                              tcCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAaca                            >  3:45063/1‑149 (MQ=255)
                                                                                                              tcCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAaca                            >  6:36770/1‑149 (MQ=255)
                                                                                                                cATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGaaaa                              <  2:87594/145‑1 (MQ=255)
                                                                                                                 aTGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCt                         >  5:297727/1‑149 (MQ=255)
                                                                                                                     gAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACCCCtt                        >  6:197999/1‑146 (MQ=255)
                                                                                                                     gAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCtt                        <  5:197999/146‑1 (MQ=255)
                                                                                                                     gAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCgg                     <  6:202762/149‑1 (MQ=255)
                                                                                                                     gAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCgg                     >  6:421542/1‑149 (MQ=255)
                                                                                                                     gAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCgg                     <  2:59015/149‑1 (MQ=255)
                                                                                                                     gAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCgg                     <  5:421542/149‑1 (MQ=255)
                                                                                                                     gAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCAGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCgg                     >  2:418996/1‑149 (MQ=255)
                                                                                                                     gAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGACCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCgg                     >  5:202762/1‑149 (MQ=255)
                                                                                                                            aaGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAAt              >  5:125207/1‑149 (MQ=255)
                                                                                                                             aGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTGCGGGGAAAATc             <  6:56683/149‑1 (MQ=255)
                                                                                                                                 aCGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGaaa         <  5:311913/149‑1 (MQ=255)
                                                                                                                                  cGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAAt        <  1:128435/149‑1 (MQ=255)
                                                                                                                                   gTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCcaca                                               >  6:282043/1‑109 (MQ=255)
                                                                                                                                   gTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCcaca                                               <  5:282043/109‑1 (MQ=255)
                                                                                                                                        ccTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTc  >  7:117908/1‑149 (MQ=255)
                                                                                                                                        ccTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTc  <  2:135497/149‑1 (MQ=255)
                                                                                                                                        ccTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTc  >  1:135497/1‑149 (MQ=255)
                                                                                                                                          tGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGa                                                     <  2:137885/96‑1 (MQ=255)
                                                                                                                                          tGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTACCGCGCATTCCGTGAAGa                                                     >  1:137885/1‑96 (MQ=255)
                                                                                                                                                 |                                                                                                                                           
TGAACGCAAACCCATCTACTGGATGCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTC  >  NC_000913/1662205‑1662489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: