Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,757,419 T→G 5.0% intergenic (+19/+225) intA → / ← yfjH CP4‑57 prophage; integrase/CP4‑57 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,757,4190TG 5.0% 275.1 / 4.3 92intergenic (+19/+225)intA/yfjHCP4‑57 prophage; integrase/CP4‑57 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (36/51);  new base G (3/2);  total (39/53)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CGCTTATAACCGCAGTGATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCTGTGGGGTGTTGCTCCTTTT  >  NC_000913/2757271‑2757566
                                                                                                                                                    |                                                                                                                                                   
cgcTTATAACCGCAGTGATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGct                                                                                                                                                     >  7:189412/1‑149 (MQ=255)
  cTTATAACCGCAGTGATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGctct                                                                                                                                                   <  3:136225/149‑1 (MQ=255)
        aCCGCAGTGATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTggg                                                                                                                                              <  1:284190/148‑1 (MQ=255)
        aCCGCAGTGATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGc                                                                                                                                             <  8:76257/149‑1 (MQ=255)
         ccGCAGTGATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCt                                                                                                                                            >  2:190144/1‑149 (MQ=255)
            cAGAGATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGAAAAATTCGTCTTGTCGGATAAAATATTTATAAGACAATCTCTCTGGTCTGa                                                                                                                                          >  1:11567/1‑148 (MQ=255)
               tGATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATAtt                                                                                                                                      >  7:57474/1‑149 (MQ=255)
               tGATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCGGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGAGAGCTGGGCTGataaa                                                                                                                                      <  5:228112/149‑3 (MQ=255)
                 aTTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGCTAGCATAGTTTTAACGCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTc                                                                                                                                    <  7:277910/149‑1 (MQ=255)
                 aTTATCTTGAGCAACGGCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATAttt                                                                                                                                     <  1:145009/148‑1 (MQ=255)
                  ttATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTc                                                                                                                                    >  8:323969/1‑148 (MQ=255)
                    aTCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGc                                                                                                                                             <  5:282996/137‑1 (MQ=255)
                    aTCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGc                                                                                                                                             >  6:282996/1‑137 (MQ=255)
                     tCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGAGAGCTGGGCTGataaatattt                                                                                                                                 <  4:59021/148‑8 (MQ=255)
                        tGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTaa                                                                                                                                 >  7:10449/1‑145 (MQ=255)
                         gAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTaaaa                                                                                                                               <  8:324002/146‑1 (MQ=255)
                         gAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGa                                                                                                                             <  3:141708/148‑1 (MQ=255)
                         gAGCAACGTCGTCCGATGAGGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTaaaa                                                                                                                               <  5:15934/146‑1 (MQ=255)
                             aaCGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCa                                                                                                                        <  4:130100/149‑1 (MQ=255)
                              aCGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGctctc                                                                                                                                                  >  1:304963/1‑122 (MQ=255)
                              aCGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGctctc                                                                                                                                                  <  2:304963/122‑1 (MQ=255)
                                   gtcCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAAt                                                                                                                  <  3:37016/149‑1 (MQ=255)
                                   gtcCGATGATGCAATGGTGCGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGATCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAATATCGCACTaaa                                                                                                                   <  8:23773/148‑1 (MQ=255)
                                    tcCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTATCATCTTTTTAACTCATTTGAGCAAAATTAGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATa                                                                                                                 <  3:278981/149‑1 (MQ=255)
                                             gCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTAcgcg                                                                                                         >  3:205890/1‑148 (MQ=255)
                                                 tggtggGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATa                                                                                                    <  3:223499/149‑1 (MQ=255)
                                                       gCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGctc                                                                                              <  8:312313/149‑1 (MQ=255)
                                                       gCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGctc                                                                                              <  5:332477/149‑1 (MQ=255)
                                                         tGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTa                                                                                            >  1:1139/1‑149 (MQ=255)
                                                         tGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTa                                                                                            >  2:109890/1‑149 (MQ=255)
                                                          gATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTAc                                                                                           >  8:220646/1‑149 (MQ=255)
                                                           aTCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACt                                                                                          >  8:323099/1‑149 (MQ=255)
                                                              tcGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTAATTTGAGCAAAAAACGTCTTGTCGGATAAAATATTTATCAGCCAAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACAAAATAGATACGAGGAATAGTGCTCTACTTTc                                                                                       >  3:172145/1‑149 (MQ=255)
                                                              gcGTAAACGCAGCAGATCGTGGTAGCATCGTTTTAACTCATTTGAGCAACCTTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACtt                                                                                         <  4:317815/146‑1 (MQ=255)
                                                                gTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTc                                                                                       <  1:283949/147‑1 (MQ=255)
                                                                gTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTc                                                                                       <  5:114285/147‑1 (MQ=255)
                                                                gTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGt                                                                                     >  5:179166/1‑149 (MQ=255)
                                                                   aaaGCAGCAGTTAGTGGTAGCATAGTTTTAACTCATTTGAGCAAAATTCGTCTTTTCGGATAAAATATTTATCAGCCCAGCTCTCTTGTCTGATATTTCTAAAAGATCGCACTAAATATCTACGCTTAATAGTGCTCTACTTTCGTTTa                                                                                  <  2:11567/149‑1 (MQ=255)
                                                                   aaaGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCATCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTa                                                                                  <  3:57924/149‑1 (MQ=255)
                                                                      gcagcaGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGcc                                                                               <  4:277493/149‑1 (MQ=255)
                                                                       cagcagATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGccc                                                                              >  3:205191/1‑149 (MQ=255)
                                                                          cagATAGTGGTAGCATCGTTTTAACTCAATTGAGCAAAATTCGTCTCGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCttt                                                                           <  4:213373/149‑1 (MQ=255)
                                                                            gATAGTGGTAGCATCTTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCttt                                                                           >  5:92030/1‑147 (MQ=255)
                                                                            gATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCa                                                                         <  7:243193/149‑1 (MQ=255)
                                                                            gATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCa                                                                         <  8:243637/149‑1 (MQ=255)
                                                                                gTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTc                                                                                       >  8:73045/1‑131 (MQ=255)
                                                                                gTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTc                                                                                       <  7:73045/131‑1 (MQ=255)
                                                                                 tGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGt                                                                    <  6:179166/149‑1 (MQ=255)
                                                                                 tGGTAGCATCGTATTATCTCATTTGAGCAAAATTCGTCTTGTCGGATATAATATATATCTGCCCAGCTGTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCTGAATACTGCGCTACTTTAGTTTAGCCCTTTCAGAgg                                                                     <  4:51241/148‑1 (MQ=255)
                                                                                    tAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTaa                                                                  <  7:323099/148‑1 (MQ=255)
                                                                                    tAGCATCGTTTTAACTCATTTGAGAAAAATTAGTCTTGTCGGATAAAATCTATATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTACATTGCTACGCGGATTAGTGCTCTAATTGCGTTTAGCGCTTTCAGAGGTAAg                                                                 <  8:262429/149‑1 (MQ=255)
                                                                                       cATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGctc                                                                                              >  5:61355/1‑117 (MQ=255)
                                                                                       cATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGctc                                                                                              <  6:61355/117‑1 (MQ=255)
                                                                                       cATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACa                                                              >  4:202940/1‑149 (MQ=255)
                                                                                        aTCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCa                                                                                                                        >  6:10425/1‑90 (MQ=255)
                                                                                        aTCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCa                                                                                                                        <  5:10425/90‑1 (MQ=255)
                                                                                          cGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGTTAAGACAt                                                             >  4:153503/1‑147 (MQ=255)
                                                                                           gTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTa                                                           <  7:248587/148‑1 (MQ=255)
                                                                                           gTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTa                                                           <  4:205890/148‑1 (MQ=255)
                                                                                           gTTTTAACTCATTTGAGCAAAATTAGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCAGTAAATAGATACGCGGAATATTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAg                                                          <  1:27736/149‑1 (MQ=255)
                                                                                                aaCTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCaa                                                     >  5:295776/1‑149 (MQ=255)
                                                                                                   tCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAAtt                                                   >  2:235343/1‑148 (MQ=255)
                                                                                                   tCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATAAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAAtt                                                   >  8:230791/1‑148 (MQ=255)
                                                                                                     aTTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCATTTCAGAGGTAAGACATTAGGGCAAttt                                                  <  1:190144/147‑1 (MQ=255)
                                                                                                        tGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACt                                              <  7:220646/148‑1 (MQ=255)
                                                                                                            cAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTAt                                          >  3:297230/1‑148 (MQ=255)
                                                                                                             aaaaTTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGa                                        >  2:125402/1‑149 (MQ=255)
                                                                                                              caaTTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTCGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGAc                                       >  7:241060/2‑149 (MQ=255)
                                                                                                                 ttCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTgc                                    >  6:43448/1‑149 (MQ=255)
                                                                                                                 ttCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTgc                                    <  1:140702/149‑1 (MQ=255)
                                                                                                                       ttGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGt                               >  7:73014/1‑148 (MQ=255)
                                                                                                                         gTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCAt                            >  3:136636/1‑149 (MQ=255)
                                                                                                                          tCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATc                           <  8:57474/149‑1 (MQ=255)
                                                                                                                          tCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATc                           <  1:210385/149‑1 (MQ=255)
                                                                                                                          tCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATc                           >  8:76276/1‑149 (MQ=255)
                                                                                                                          tCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATc                           >  4:78908/1‑149 (MQ=255)
                                                                                                                          tCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATc                           <  1:109890/149‑1 (MQ=255)
                                                                                                                            ggATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGa                         <  7:323969/149‑1 (MQ=255)
                                                                                                                            ggATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGa                         <  3:130448/149‑1 (MQ=255)
                                                                                                                            ggATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGa                         <  4:172095/149‑1 (MQ=255)
                                                                                                                             gATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAg                        >  3:304851/1‑149 (MQ=255)
                                                                                                                                 ttagaaatATCAGCCCAGAGAGCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCtg                    >  7:169956/8‑149 (MQ=255)
                                                                                                                                 aaaTATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCt                     <  5:43448/148‑1 (MQ=255)
                                                                                                                                  aaTATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCtgt                   <  3:202940/149‑1 (MQ=255)
                                                                                                                                   atatTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCtgtg                  <  7:76276/149‑1 (MQ=255)
                                                                                                                                   atatTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCtgtg                  <  2:1139/149‑1 (MQ=255)
                                                                                                                                   atatTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCtgtg                  <  1:235343/149‑1 (MQ=255)
                                                                                                                                       atATCAGCCCAGAGAGCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCTGTGGGgt              >  5:280463/2‑149 (MQ=255)
                                                                                                                                        tATCAGCCCAGCTCTCTTGGAGGATATTTCTAAAAGATAGCTCTAAATAGCTAAGCGGAATAGTGCGCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCTCAGTCATCGAGTCTGTGGGgt              <  4:172145/148‑1 (MQ=255)
                                                                                                                                        tATCAGCCCAGAGAGCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGCTTCTGTGGGgtg             >  5:308216/1‑149 (MQ=255)
                                                                                                                                         aTCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCTGTGGGgtgt            >  7:87586/1‑149 (MQ=255)
                                                                                                                                             gCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCTGTGGGGTGTTGCt        >  6:275966/1‑149 (MQ=255)
                                                                                                                                              cccAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCTGTGGGGTGTTGCTc       >  7:59468/1‑149 (MQ=255)
                                                                                                                                               ccAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCTGTGGGGTGTTGCTcc      >  6:241969/1‑149 (MQ=255)
                                                                                                                                                 aGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCTGTGGGGTGTTGCTCCtt    <  8:189412/149‑1 (MQ=255)
                                                                                                                                                 aGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCTGTGGGGTGTTGCTCCtt    <  6:295776/149‑1 (MQ=255)
                                                                                                                                                   ctctctGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCTGTGGGGTGTTGCTCCtttt  <  1:125402/149‑1 (MQ=255)
                                                                                                                                                   ctctctGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCTGTGGGGTGTTGCTCCtttt  <  7:230791/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGCTTATAACCGCAGTGATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCTGTGGGGTGTTGCTCCTTTT  >  NC_000913/2757271‑2757566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑