Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,894,735 T→A 5.9% intergenic (‑4/‑70) yoaH ← / → pabB UPF0181 family protein/aminodeoxychorismate synthase, subunit I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,894,7350TA 5.9% 204.0 / 2.7 68intergenic (‑4/‑70)yoaH/pabBUPF0181 family protein/aminodeoxychorismate synthase, subunit I
Reads supporting (aligned to +/- strand):  ref base T (30/34);  new base A (2/2);  total (32/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01

GCCGGAGTGGTTGGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGC  >  NC_000913/1894591‑1894882
                                                                                                                                                |                                                                                                                                                   
gcCGGATTGGTTTTCAGGCAGCTCTTCTGCCACCAGCGCGAGTGCCTGGCAGCTGCTCATCCATTCTGACAGCAGTTCCTGGATCCGCGAGACAGCGTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAATCATTGTTaat                                                                                                                                                  <  8:171060/148‑2 (MQ=255)
gcCGGAGTGGTTGGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTaa                                                                                                                                                   <  4:289358/147‑1 (MQ=255)
         gTTGGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTaaa                                                                                                                                        <  7:258308/149‑1 (MQ=255)
         gTTGGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTaaa                                                                                                                                        >  4:81181/1‑149 (MQ=255)
           tGGCACGCAGCTCTGCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAAtt                                                                                                                                      <  8:257079/149‑1 (MQ=255)
            ggCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTTCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAAtt                                                                                                                                      <  7:165122/148‑1 (MQ=255)
            ggCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTACCTCCTGCTAAAttg                                                                                                                                     >  4:194918/1‑149 (MQ=255)
            ggCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAAtt                                                                                                                                      <  3:326142/148‑1 (MQ=255)
               aCGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAAttgttg                                                                                                                                  <  6:144848/149‑1 (MQ=255)
                 gCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGgc                                                                                                                                <  2:64590/149‑1 (MQ=255)
                   aGCTCTTCTGCCACCAGCGCGATTGCCTGGCGGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTGGAGAGCTTTTTTCTGCTGTTCATGTGTGAGTGAATGTATACCTGCAAAGATTGTTAACTCGTGCTAAAATGTTGgcgc                                                                                                                              <  4:280352/149‑1 (MQ=255)
                   aGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCCGCTCATCCCTTGTGCGATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGACGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGgcgc                                                                                                                              >  2:316630/1‑149 (MQ=255)
                        ttctGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAat                                                                                                                          >  6:262036/1‑148 (MQ=255)
                         tctGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAatta                                                                                                                        >  7:119873/1‑149 (MQ=255)
                          ctGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAatta                                                                                                                        <  7:52898/148‑1 (MQ=255)
                           tGCCACCAGCCGGATTGCCTGGCCGCTGCTCATCCCATGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTTTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAattatt                                                                                                                      <  6:22792/149‑1 (MQ=255)
                             ccaccaGCGCGATTGCCTGGCCGCTGCTCATGCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAAt                                                                                                                                       <  7:49859/130‑1 (MQ=255)
                             ccaccaGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAAt                                                                                                                                       >  8:49859/1‑130 (MQ=255)
                                ccaTCGCGATTTCCTTGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGAAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCCTg                                                                                                                 <  5:250891/149‑1 (MQ=255)
                                ccaGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTAAACTCCTGCTAAATTGTTGgcg                                                                                                                               <  4:5575/135‑1 (MQ=255)
                                ccaGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTAAACTCCTGCTAAATTGTTGgcg                                                                                                                               >  3:5575/1‑135 (MQ=255)
                                  aGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGc                                                                                                                >  5:304970/1‑148 (MQ=255)
                                            ccTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGcaca                                                                                                     >  4:134839/1‑149 (MQ=255)
                                            ccTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATACGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCGAAATTGTTGGCGCGTATTATTTCATGCTACCCGGccca                                                                                                     >  4:95169/1‑149 (MQ=255)
                                                gCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGc                                                                                                 >  8:209926/1‑149 (MQ=255)
                                                       ctCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTACACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTaga                                                                                          <  2:242722/149‑1 (MQ=255)
                                                        tCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTagag                                                                                         >  1:241188/1‑149 (MQ=255)
                                                          aTCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGt                                                                                        >  8:297816/1‑148 (MQ=255)
                                                           tCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTTCCTCCTGCTAAATTGGTGGCGCTAATTATTTCATGCTACCCGGCCCAAAGCCAGTAGAGt                                                                                        >  2:86427/1‑147 (MQ=255)
                                                           tCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCa                                                                                      <  6:133005/149‑1 (MQ=255)
                                                             ccTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAgg                                                                                    >  2:40833/1‑149 (MQ=255)
                                                               ttGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCCGCAAACATTGTAAACTCCTGCCAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAg                                                                                     <  5:145021/146‑1 (MQ=255)
                                                               ttGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCCGCAAACATTGTAAACTCCTGCAAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAg                                                                                     >  6:145021/1‑146 (MQ=255)
                                                                 gtgCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGATGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTATCTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACt                                                                                 >  6:252826/1‑148 (MQ=255)
                                                                  tgCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACtga                                                                               <  3:81181/149‑1 (MQ=255)
                                                                    ccATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACtgat                                                                              >  1:272818/1‑148 (MQ=255)
                                                                     cATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACtgatga                                                                            <  7:209926/149‑1 (MQ=255)
                                                                     cATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGGTCATGGGTGAGGGACGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACtgatga                                                                            <  8:144356/149‑1 (MQ=255)
                                                                      aTCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGaa                                                                           <  7:340187/149‑1 (MQ=255)
                                                                       tCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACtgatg                                                                             >  2:91988/1‑146 (MQ=255)
                                                                       tCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACtgatg                                                                             >  5:291865/1‑146 (MQ=255)
                                                                       tCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAg                                                                          <  2:272818/149‑1 (MQ=255)
                                                                          gTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACg                                                                       <  1:10325/149‑1 (MQ=255)
                                                                           ttCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGt                                                                      >  6:249275/1‑149 (MQ=255)
                                                                                   tCCGCTCTACAGCTTTTTGCTGCTGTTAATGGGTGAGTGAAGGTAAACCTGCAAACATAGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTcc                                                               <  1:159446/148‑1 (MQ=255)
                                                                                      gCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCt                                                           >  3:84747/1‑149 (MQ=255)
                                                                                      gCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCt                                                           >  6:100366/1‑149 (MQ=255)
                                                                                             aGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGAtta                                                    >  8:331459/1‑149 (MQ=255)
                                                                                              gCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACACGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGAttac                                                   >  3:319816/1‑149 (MQ=255)
                                                                                               cTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTACTTATTTCATGCTACCCGGCACATAGCCAGTAGAGGCAGGACTGATGAAGACGTTTTCTCCCGCTGTGAttact                                                  <  3:275535/149‑1 (MQ=255)
                                                                                                  tttGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGAttacttta                                               >  8:139084/1‑149 (MQ=255)
                                                                                                   ttGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCa                                                                                               >  2:59710/1‑100 (MQ=255)
                                                                                                   ttGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCa                                                                                               <  1:59710/100‑1 (MQ=255)
                                                                                                         ctgTTCATGGGTGAGTGAAGGTAAAGCTGCAAAGATTGTTAACTCCTGCTATATTGTTGGCGCTAATTATTTCATGCTACACGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGc                                        <  3:256948/149‑1 (MQ=255)
                                                                                                         ctgTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGc                                        <  3:134839/149‑1 (MQ=255)
                                                                                                               aTGGGTGAGGGAAGGTAAACCTGCAACCATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCGACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAg                                   <  3:194918/148‑1 (MQ=255)
                                                                                                                      agtgaAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGAcgccgc                           >  3:259091/1‑149 (MQ=255)
                                                                                                                       gtgaAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGTACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGAcgccgc                           <  5:138448/148‑1 (MQ=255)
                                                                                                                           aGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAAt                      >  5:332864/1‑149 (MQ=255)
                                                                                                                                aaCCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTa                  <  3:141194/148‑1 (MQ=255)
                                                                                                                                 aCCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTAtt                <  4:193049/149‑1 (MQ=255)
                                                                                                                                   cTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGCCTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTtc              <  5:262036/149‑1 (MQ=255)
                                                                                                                                   cTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTtc              >  2:152289/1‑149 (MQ=255)
                                                                                                                                    tGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTtct             >  4:265568/1‑149 (MQ=255)
                                                                                                                                     gCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGCAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTtctc            >  2:127003/1‑149 (MQ=255)
                                                                                                                                      cAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTtct             <  5:100366/147‑1 (MQ=255)
                                                                                                                                              gTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTaa    <  7:331459/148‑1 (MQ=255)
                                                                                                                                              gTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTaa    <  1:258425/148‑1 (MQ=255)
                                                                                                                                               ttAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGc  <  1:86427/149‑1 (MQ=255)
                                                                                                                                               ttAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGc  <  4:259091/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GCCGGAGTGGTTGGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGC  >  NC_000913/1894591‑1894882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑