Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,807,100	C→T	6.6%	P244L (CCA→CTA)	pfkB →	6‑phosphofructokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,807,100	0	C	T	6.6%	236.5 / 4.8	76	P244L (CCA→CTA)	pfkB	6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base C (32/39);  new base T (2/3);  total (34/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.10e-01

AATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAA  >  NC_000913/1806955‑1807243                                                                                                                                                  |                                                                                                                                                aaTCGCGAACTCACCCCGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGGCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCcaccac                                                                                                                                               <  3:142418/148‑1 (MQ=255) aaTCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCaccacc                                                                                                                                              >  5:237023/1‑149 (MQ=255) aaTCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAACGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGGTGATAGTGAAAACTGTATTCAGGTGGTGCCaccacc                                                                                                                                              <  1:323891/149‑1 (MQ=255)   tCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCgg                                                                                                                                            <  6:97836/149‑1 (MQ=255)      cgAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGTCCAAACCGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGCTGCCACCACCGGTg                                                                                                                                          >  4:296480/1‑148 (MQ=255)         aCTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGaaaa                                                                                                                                      <  6:128120/149‑1 (MQ=255)           tCACCCCGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGc                                                                                                                                    >  4:196370/1‑149 (MQ=255)           tCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGc                                                                                                                                    >  7:175413/1‑149 (MQ=255)               ccAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCaga                                                                                                                                <  6:236041/149‑1 (MQ=255)               ccAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCaga                                                                                                                                <  2:266271/149‑1 (MQ=255)                      gACGATGTCCGCAAAGCCGCGCAGGAAAGCGTTACTAGCGGCAAGGCCAAACGGGTTCGCGTTTCACAGGGTCCACAAGGAGCGCTGGGGGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGt                                                                                                                         <  7:239514/149‑1 (MQ=255)                      gACGATGACAGCAACGCCGCGCAGGCAATCGTTAATAGCTGCAAGGCCAATCGGGTTGTCGTTTCCCAGTGTCCACAACGAGCGCTGGGTGTTGCTAGGGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGGACCgg                                                                                                                         <  7:239513/149‑2 (MQ=255)                       aCGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGtt                                                                                                                        <  5:257683/149‑1 (MQ=255)                               cGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTgg                                                                                                                >  8:29815/1‑149 (MQ=255)                               cGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTg                                                                                                                 <  8:57000/148‑1 (MQ=255)                                  aaaGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGa                                                                                                             >  4:267681/1‑149 (MQ=255)                                     gCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAg                                                                                                          <  8:138699/149‑1 (MQ=255)                                        gcgcAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCAt                                                                                                       <  6:237023/149‑1 (MQ=255)                                         cgcAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATg                                                                                                      <  8:327517/149‑1 (MQ=255)                                            aGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTc                                                                                                   >  4:72607/1‑149 (MQ=255)                                              gAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCgg                                                                                                 >  5:253188/1‑149 (MQ=255)                                              gAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGGGAAAACTGTATTCAGGTGTTGCCACCACCGGTGAAAAGCAAGAGTACCGTTGGCGCTGGTGACCGCTTGGTCgg                                                                                                 >  7:255782/1‑149 (MQ=255)                                                  tCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAACCTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGGCGgcgc                                                                                              >  4:5712/1‑148 (MQ=255)                                                  tCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCaga                                                                                                                                >  3:181048/1‑114 (MQ=255)                                                  tCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCaga                                                                                                                                <  4:181048/114‑1 (MQ=255)                                                    gTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGa                                                                                            <  3:72607/148‑1 (MQ=255)                                                    gTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGAGCTGGTGACAGCATGGTCGGCGCGAt                                                                                           >  2:272948/1‑149 (MQ=255)                                                       aaTAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGa                                                                                         <  3:61305/148‑1 (MQ=255)                                                       aaTAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGGGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGa                                                                                         <  1:269499/148‑1 (MQ=255)                                                        aTAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACTACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGaca                                                                                       <  3:250882/149‑1 (MQ=255)                                                        aTAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGGGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACTACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGaca                                                                                       <  1:46010/149‑1 (MQ=255)                                                        aTAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGaca                                                                                       >  4:163263/1‑149 (MQ=255)                                                        aTAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGaca                                                                                       <  8:175413/149‑1 (MQ=255)                                                           gCGGCAATCCCTAACGCGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATATTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACAGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTg                                                                                    <  6:7167/149‑1 (MQ=255)                                                              gCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGaaa                                                                                 <  1:295540/149‑1 (MQ=255)                                                              gCAAGGCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCCCCGGTGATAAGCCAGAGTACCGTTGGCGCTGGTGACGGCATGGTCGGCGCGCTGACACTGaaa                                                                                 >  3:257820/1‑149 (MQ=255)                                                                 aGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTg                                                                                                                 >  8:223533/1‑114 (MQ=255)                                                                 aGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTg                                                                                                                 <  7:223533/114‑1 (MQ=255)                                                                  ggCCAAACGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGGGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTgg                                                                             <  1:23003/149‑1 (MQ=255)                                                                         cGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGGGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGaaaa                                                                      <  8:273902/149‑1 (MQ=255)                                                                         cGGGTTGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGaaaa                                                                      >  1:178272/1‑149 (MQ=255)                                                                             ttGTCGTTTCCCAGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGgc                                                                                                <  8:46956/119‑1 (MQ=255)                                                                                tCGTTTCCCAGGGTCCACAAGGAGAGCTTGGAGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCctc                                                                >  8:77079/1‑148 (MQ=255)                                                                                       ccagggTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGaa                                                         <  3:296480/145‑1 (MQ=255)                                                                                       ccagggTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAag                                                        <  8:262756/146‑1 (MQ=255)                                                                                       ccTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTg                                                           >  8:5355/1‑146 (MQ=255)                                                                                       ccTGGGTCCACAAGGAGCGCGGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGaa                                                         <  3:196370/148‑1 (MQ=255)                                                                                         tGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGaa                                                         >  7:186345/1‑146 (MQ=255)                                                                                         agggTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGaa                                                         >  7:305860/2‑146 (MQ=255)                                                                                           ggTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGAt                                                    >  4:252663/1‑149 (MQ=255)                                                                                           ggTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGAt                                                    >  8:11462/1‑149 (MQ=255)                                                                                                  aaGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGt                                             >  5:9333/1‑149 (MQ=255)                                                                                                      aGCGCTGGGTGTTGATAGTGAAAACTTTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTg                                         <  2:90075/149‑1 (MQ=255)                                                                                                      aGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTg                                         <  3:167129/149‑1 (MQ=255)                                                                                                       gcgcTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACTACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATTCCTCTCTTGAAGAGATGGTTCGTTTTgg                                        >  3:266072/1‑149 (MQ=255)                                                                                                       gcgcTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTgg                                        >  7:171394/1‑149 (MQ=255)                                                                                                          cTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGt                                     <  8:171394/149‑1 (MQ=255)                                                                                                             ggTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAACAGCCAGAGTACCGTTGGCGCTGGTGACAGCAAGGTCGGCGCGATGACCCTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGc                                  >  6:294146/1‑149 (MQ=255)                                                                                                             ggTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTg                                         >  5:334687/1‑142 (MQ=255)                                                                                                             ggTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTg                                         <  6:334687/142‑1 (MQ=255)                                                                                                             ggTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTa                                    <  7:29815/147‑1 (MQ=255)                                                                                                              gtgtTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCt                                 >  1:14483/1‑149 (MQ=255)                                                                                                                   gATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCgg                             <  1:272948/148‑1 (MQ=255)                                                                                                                   gATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGGCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCgg                             <  8:255782/148‑1 (MQ=255)                                                                                                                    aTAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCggg                            >  2:184916/1‑148 (MQ=255)                                                                                                                     tAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCggg                            >  6:230015/1‑147 (MQ=255)                                                                                                                        tGAAAACTGTATTCAGGTGGTGCCACTACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCTTGGTCGGCGCGATGACACTGAAACTCGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTg                       <  8:228528/149‑1 (MQ=255)                                                                                                                        tGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTg                       <  3:267681/149‑1 (MQ=255)                                                                                                                        tGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTg                       >  1:81837/1‑149 (MQ=255)                                                                                                                        tGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTg                       <  2:14483/149‑1 (MQ=255)                                                                                                                           aaaCTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAg                    <  2:178272/149‑1 (MQ=255)                                                                                                                             aCTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGGGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGcc                  <  8:166956/149‑1 (MQ=255)                                                                                                                              cTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGATATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCca                 >  3:271338/1‑149 (MQ=255)                                                                                                                                      aGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAAt         >  3:208317/1‑149 (MQ=255)                                                                                                                                      aGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAAt         <  7:5355/149‑1 (MQ=255)                                                                                                                                        gtggtgCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAAt         >  4:155044/1‑147 (MQ=255)                                                                                                                                          ggtgCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAgg     <  6:78190/149‑1 (MQ=255)                                                                                                                                           gtgCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAgg     <  7:77079/148‑1 (MQ=255)                                                                                                                                             gCCACTACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGaa  >  5:268688/1‑149 (MQ=255)                                                                                                                                              ccaccaCCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGaa  <  3:252663/148‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                AATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAA  >  NC_000913/1806955‑1807243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑