Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,700,680 C→T 5.5% A442A (GCC→GCT malX → maltose and glucose‑specific PTS enzyme IIB component and IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,700,6800CT 5.5% 233.9 / 2.2 73A442A (GCC→GCTmalXmaltose and glucose‑specific PTS enzyme IIB component and IIC component
Reads supporting (aligned to +/- strand):  ref base C (34/34);  new base T (2/2);  total (37/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.04e-01

TGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGG  >  NC_000913/1700532‑1700828
                                                                                                                                                    |                                                                                                                                                    
tGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGcc                                                                                                                                                      >  1:263505/1‑149 (MQ=255)
tGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGcc                                                                                                                                                      >  5:115942/1‑149 (MQ=255)
  tCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGcc                                                                                                                                                      <  8:169507/147‑1 (MQ=255)
    aaCCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACTTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTg                                                                                                                                                  <  6:303809/149‑1 (MQ=255)
      ccAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTgcg                                                                                                                                                >  6:262968/1‑149 (MQ=255)
                cATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAAt                                                                                                                                      <  3:137718/149‑1 (MQ=255)
                    gTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAgg                                                                                                                                  <  6:76612/149‑1 (MQ=255)
                          gtggtgGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACaa                                                                                                                            >  2:145030/1‑149 (MQ=255)
                           tggtggCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAAc                                                                                                                           <  7:324027/149‑1 (MQ=255)
                                 cggcAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCt                                                                                                                     <  4:272545/149‑1 (MQ=255)
                                  ggcAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTg                                                                                                                    >  3:61781/1‑149 (MQ=255)
                                         tGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCt                                                                                                             >  7:6656/1‑149 (MQ=255)
                                         tGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCt                                                                                                             >  1:30605/1‑149 (MQ=255)
                                            tttGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCg                                                                                                           >  8:28964/1‑148 (MQ=255)
                                              tGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAg                                                                                                        >  3:154184/1‑149 (MQ=255)
                                               gTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAg                                                                                                        >  4:8072/1‑148 (MQ=255)
                                                tCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCa                                                                                                      >  2:123533/1‑149 (MQ=255)
                                                  gTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCAtt                                                                                                    <  4:154184/149‑1 (MQ=255)
                                                   tttACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTa                                                                                                   <  5:262968/149‑1 (MQ=255)
                                                       ctacGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCg                                                                                               <  1:145030/149‑1 (MQ=255)
                                                          cGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCTGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTg                                                                                            >  4:147060/1‑149 (MQ=255)
                                                            tCATCTTCCGTTTAGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTAGCCGCTGCGCCGGGTAAATCAGGTTACAACGTACCTGCAATCCTCCAAGCATTAGGCGGTGcc                                                                                          >  5:228424/1‑149 (MQ=255)
                                                              aTCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCAGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGa                                                                                        >  3:151939/1‑149 (MQ=255)
                                                                cTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGAc                                                                                       >  8:135917/1‑148 (MQ=255)
                                                                cTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGAc                                                                                       >  8:140722/1‑148 (MQ=255)
                                                                 ttCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAGGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACaa                                                                                     >  8:244251/1‑149 (MQ=255)
                                                                 ttCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACaa                                                                                     >  5:206342/1‑149 (MQ=255)
                                                                 ttCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACaa                                                                                     >  2:258332/1‑149 (MQ=255)
                                                                  tCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACaa                                                                                     >  8:95974/1‑148 (MQ=255)
                                                                   ccGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACaa                                                                                     <  4:503/147‑1 (MQ=255)
                                                                     gTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATAtt                                                                                 >  8:204583/1‑149 (MQ=255)
                                                                     gTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATAtt                                                                                 >  4:200480/1‑149 (MQ=255)
                                                                     gTTTCGCTATCACCCGCTTCAATCGGACAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTTCAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATAtt                                                                                 <  2:170368/149‑1 (MQ=255)
                                                                         cGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTc                                                                              <  4:61781/148‑1 (MQ=255)
                                                                          gCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAg                                                                            <  7:204583/149‑1 (MQ=255)
                                                                          gCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGGGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCa                                                                             <  3:283769/148‑1 (MQ=255)
                                                                            tATCACCCGCTTCAATCTGAAACCCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGcc                                                                          >  6:160151/1‑149 (MQ=255)
                                                                              tCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGcc                                                                          <  6:115942/147‑1 (MQ=255)
                                                                              tCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTc                                                                        >  5:72588/1‑149 (MQ=255)
                                                                                 cccGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGa                                                                      >  4:241451/1‑148 (MQ=255)
                                                                                   cGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATaa                                                                   <  6:252460/149‑1 (MQ=255)
                                                                                   cGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATaa                                                                   <  1:46180/149‑1 (MQ=255)
                                                                                     cTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCTGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACt                                                                 <  3:147060/149‑1 (MQ=255)
                                                                                      ttCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCg                                                                                         >  2:74255/1‑124 (MQ=255)
                                                                                      ttCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCg                                                                                         <  1:74255/124‑1 (MQ=255)
                                                                                        cAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGc                                                               <  7:28964/148‑1 (MQ=255)
                                                                                          aTCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCAtt                                                            <  2:263505/149‑1 (MQ=255)
                                                                                           tCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCTGGTGCGCCGGGTAAATCAGGTTACAACGt                                                                                                                         <  2:85551/87‑1 (MQ=255)
                                                                                           tCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCTGGTGCGCCGGGTAAATCAGGTTACAACGt                                                                                                                         >  1:85551/1‑87 (MQ=255)
                                                                                           tCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGTTGCGCCGGGTAAATCAGGATACAACGTTCCTGCACTCCTAGAAGCATTAGGCGGTGCCGCCAAAATTGTCAGCCTCGATAACTGCAATa                                                           >  5:185236/1‑149 (MQ=255)
                                                                                           tCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTa                                                           >  5:58079/1‑149 (MQ=255)
                                                                                              gAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTAccc                                                        >  3:194230/1‑149 (MQ=255)
                                                                                               aaaaCCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGATCCTGCAATCCTCGACGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTAc                                                          <  3:303192/146‑1 (MQ=255)
                                                                                                 aaCCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTc                                                     >  4:251624/1‑149 (MQ=255)
                                                                                                      cGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGt                                                <  1:258332/149‑1 (MQ=255)
                                                                                                        gggCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGttt                                              <  4:151939/149‑1 (MQ=255)
                                                                                                           cgcgATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTc                                           <  3:8072/149‑1 (MQ=255)
                                                                                                            gcgATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCt                                          <  3:19720/149‑1 (MQ=255)
                                                                                                            gcgATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCt                                          >  2:21253/1‑149 (MQ=255)
                                                                                                             cgATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCtg                                         >  2:340165/1‑149 (MQ=255)
                                                                                                                 aGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCAGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGa                                      >  6:192238/1‑148 (MQ=255)
                                                                                                                    gAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATCAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGa                                      >  6:77019/1‑145 (MQ=255)
                                                                                                                      aGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGata                                <  2:30605/149‑1 (MQ=255)
                                                                                                                       gTTGCCAGCTCCATCGAACAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGGTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGatat                               <  2:159855/149‑1 (MQ=255)
                                                                                                                            cAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGc                          <  3:200480/149‑1 (MQ=255)
                                                                                                                             aGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGc                                                               >  3:233168/1‑111 (MQ=255)
                                                                                                                             aGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGc                                                               <  4:233168/111‑1 (MQ=255)
                                                                                                                              gCTCAATCGAAAACGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCtt                        <  5:322147/149‑1 (MQ=255)
                                                                                                                              gCTCAATCGAAAAAGCCGTTGCCGGTTCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTGTAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCtt                        <  6:228424/149‑1 (MQ=255)
                                                                                                                                   aTCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTaa                   <  1:123533/149‑1 (MQ=255)
                                                                                                                                     cGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAAtg                 >  2:46688/1‑149 (MQ=255)
                                                                                                                                       aaaaaGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAAtgtg               >  2:224710/1‑149 (MQ=255)
                                                                                                                                        aaaaGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGc              <  5:160151/149‑1 (MQ=255)
                                                                                                                                         aaaGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGc                          <  7:56662/136‑1 (MQ=255)
                                                                                                                                         aaaGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGc                          >  8:56662/1‑136 (MQ=255)
                                                                                                                                              cGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGGCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCAc        <  2:55211/149‑1 (MQ=255)
                                                                                                                                                   ccGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGaa    <  7:135917/148‑1 (MQ=255)
                                                                                                                                                   ccGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGaa    <  7:95974/148‑1 (MQ=255)
                                                                                                                                                   ccGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAg   <  4:302624/149‑1 (MQ=255)
                                                                                                                                                   ccGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAg   <  8:6656/149‑1 (MQ=255)
                                                                                                                                                   ccGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTCAATGTGCAGGCACTGaa    <  7:140722/148‑1 (MQ=255)
                                                                                                                                                    cGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCAATAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCAATACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAgg  >  2:174050/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTAATGTGCAGGCACTGAAGG  >  NC_000913/1700532‑1700828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: