Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 176,046 G→T 5.3% G314C (GGC→TGC)  clcA → H(+)/Cl(‑) exchange transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913176,0460GT 5.3% 246.8 / 3.0 76G314C (GGC→TGC) clcAH(+)/Cl(‑) exchange transporter
Reads supporting (aligned to +/- strand):  ref base G (40/32);  new base T (2/2);  total (42/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01

CCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGCACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGTTTATCTTCGTCGCGCGGGTCATTACCACCTTACTCTGCTTCTCTTCCGGCGCGCCGGGCGGTATTTTTGCCCCGATGCTGGCGCT  >  NC_000913/175904‑176194
                                                                                                                                              |                                                                                                                                                    
cccTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGCACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGgcggcg                                                                                                                                                >  5:307007/1‑149 (MQ=255)
cccTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGCACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGgcggcg                                                                                                                                                <  3:159575/149‑1 (MQ=255)
 ccTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGCACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGcggcgg                                                                                                                                               >  2:99495/1‑149 (MQ=255)
  cTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGCACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGGGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGt                                                                                                                                              <  6:310779/149‑1 (MQ=255)
     tttttAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGCACGGCGGCAATATTACCAACTGGGTGCTCATGGGCGGTGCGATTGGCGGGCTGTGTGGAGTGCGGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGtg                                                                                                                                             <  3:29165/147‑2 (MQ=255)
       tttAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGCACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTaa                                                                                                                                         >  6:195315/1‑149 (MQ=255)
        ttAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGCACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCTGCGGTTTTAAc                                                                                                                                        >  7:241991/1‑149 (MQ=255)
          aataaATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGCACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCt                                                                                                                                      >  2:16022/1‑149 (MQ=255)
            taaATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGCACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGa                                                                                                                                    <  4:99024/149‑1 (MQ=255)
            taaATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGCACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGa                                                                                                                                    >  8:217651/1‑149 (MQ=255)
            taaATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGCACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGa                                                                                                                                    <  1:99495/149‑1 (MQ=255)
               aTGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGCACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTc                                                                                                                                 >  6:209878/1‑149 (MQ=255)
                      cTGGGGATGCAGGATTTGCTGCACCGTGTGCACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCg                                                                                                                           <  6:242111/148‑1 (MQ=255)
                      cTGGGGATGCAGGATTTGCTGCACCGTGTGCACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGc                                                                                                                          <  3:74684/149‑1 (MQ=255)
                        ggggATGCAGGATTTGCTGCACCGTGTGCACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTAt                                                                                                                             <  1:114180/144‑1 (MQ=255)
                        ggggATGCAGGATTTGCTGCACCGTGTGCACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTAt                                                                                                                             >  2:114180/1‑144 (MQ=255)
                        ggggATGCAGGATTTGCTGCACCGTGTGCACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTa                                                                                                                        <  2:183132/149‑1 (MQ=255)
                             tGCAGGATTTGCTGCACCGTGTGCACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGAGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACcgcg                                                                                                                   >  7:89387/1‑149 (MQ=255)
                               cagtctttGCTGCACCGGGTGCACGGCGGCAATATTACCAACTGGGGGCTAATGGGCGGTGCGATTGGCGGGCTGTGTGGATTGCTGGGGGTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCgg                                                                                                                  <  5:23462/143‑1 (MQ=255)
                               cAGGATTTGCTGCACCGTGTGCACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCggg                                                                                                                 <  1:16022/149‑1 (MQ=255)
                                aGGATTTGCTGCACCGTGTGCACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCggg                                                                                                                 >  1:5186/1‑148 (MQ=255)
                                 ggATTTGCTGCACCGTGTGCACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCgggg                                                                                                                >  6:228416/1‑148 (MQ=255)
                                    tttGCTGCACCGTGTGCACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAAtt                                                                                                            >  7:340190/1‑149 (MQ=255)
                                    tttGCTGCACCGTGTGCACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAAtt                                                                                                            >  3:93585/1‑149 (MQ=255)
                                    tttGCTGCACCGTGTGCACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGAGGCACCAGCAACGTCGGGCGGCGGTTTTAACATGATTACTATCGCTACCGCGGGGAAtt                                                                                                            >  2:282390/1‑149 (MQ=255)
                                       gctgcACCGTGTGCACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAAttt                                                                                                           >  7:139920/1‑147 (MQ=255)
                                        ctgcACCGTGTGCACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCa                                                                                                         <  7:138017/148‑1 (MQ=255)
                                         tgcACCGTGTGCACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAg                                                                                                        >  7:168565/1‑148 (MQ=255)
                                           cagcGTGTGCACTGCTGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCATCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGTGAATTTCAGCa                                                                                                      <  1:282390/145‑1 (MQ=255)
                                           cACCGTGTGCACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCa                                                                                                      <  8:168565/148‑1 (MQ=255)
                                           cACCGTGTGCACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCa                                                                                                      <  5:228416/148‑1 (MQ=255)
                                            aCCGTGTGCACGGAGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATg                                                                                                    <  7:254976/149‑1 (MQ=255)
                                                    cACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATgg                                                                                                   <  1:128748/142‑1 (MQ=255)
                                                    cACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATgg                                                                                                   >  2:128748/1‑142 (MQ=255)
                                                    cACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCt                                                                                            >  1:103777/1‑149 (MQ=255)
                                                         cggcAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGGCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGgtgt                                                                                       <  3:161137/149‑1 (MQ=255)
                                                            cAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGTTTa                                                                                    >  5:199587/1‑149 (MQ=255)
                                                               tatTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTTCCGCGGGGAATTTCAGCATGGGAATGCTGGTGTTTATCt                                                                                 >  3:51888/1‑149 (MQ=255)
                                                                     cAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGTTTATCTtcgtcg                                                                           >  7:180063/1‑149 (MQ=255)
                                                                       aaTGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGTGGAATTTCAGCATGGGAATGCTGGTGTTTATCTTCGTcgcg                                                                         >  8:139067/1‑149 (MQ=255)
                                                                       aaTGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGttt                                                                                     <  4:147568/137‑1 (MQ=255)
                                                                       aaTGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGttt                                                                                     >  3:147568/1‑137 (MQ=255)
                                                                           ggTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGTTTATCTTCGTCGCGCgg                                                                      <  2:34437/148‑1 (MQ=255)
                                                                            gTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAAc                                                                                                                                        >  5:15449/1‑81 (MQ=255)
                                                                            gTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAAc                                                                                                                                        <  6:15449/81‑1 (MQ=255)
                                                                               cTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGTTTATCTTCGTCGCGCGGGTCAt                                                                 <  7:9589/149‑1 (MQ=255)
                                                                                tAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGTTTATCTTCGTCGCGCGGGTCAt                                                                 >  1:30870/1‑148 (MQ=255)
                                                                                tAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGTTTATCTTCGTCGCGCGGGTCAt                                                                 >  3:305176/1‑148 (MQ=255)
                                                                                  aTGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGTTTATCTTCGTCGCGCGGGTCAtt                                                                <  5:247403/147‑1 (MQ=255)
                                                                                      gCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGTGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGTTTATCTTCGTCGCGCGGGTCATTacca                                                            <  4:66434/147‑1 (MQ=255)
                                                                                      gCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGTGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGTTTATCTTCGTCGCGCGGGTCATTacca                                                            <  4:70894/147‑1 (MQ=255)
                                                                                      gCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGTGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGTTTATCTTCGTCGCGCGGGTCATTacca                                                            >  3:70894/1‑147 (MQ=255)
                                                                                      gCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGTGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGTTTATCTTCGTCGCGCGGGTCATTacca                                                            >  3:66434/1‑147 (MQ=255)
                                                                                             gATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGTTTATCTTCGTCGCGCGGGTCATTACCACCTTActct                                                   >  6:344290/1‑149 (MQ=255)
                                                                                              aTTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGTTTATCTTCGTCGCGCGGGGCATTACGACCTTACTCTg                                                  >  8:302024/1‑149 (MQ=255)
                                                                                                  gCGGTCTGTGGGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGGGTTTATCTTCGTCGCGCGGGTCATTACCACCTTACTCTGCTtc                                              <  5:195315/149‑1 (MQ=255)
                                                                                                         gtgtgGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTg                                                                                                                                     >  4:138837/1‑55 (MQ=255)
                                                                                                         gtgtgGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTg                                                                                                                                     <  3:138837/55‑1 (MQ=255)
                                                                                                          tgtgGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGTTTATCTTCGTCGCGCGGGTCATTACCACCTTACTCTGCTTCTCTTc                                         <  2:242647/146‑1 (MQ=255)
                                                                                                           gtgGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGTTTATCTTCGTCGCGCGGGTCAGTACCACCTTACTCTGCTTCTCTTCCGgc                                     >  7:212412/1‑149 (MQ=255)
                                                                                                                  gCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGAGGGTTTCACCTGATTCCTATCGCTACCGCGGGGAATTTCATCATGGGAATGCTGGTGTTTATCTTCGTCGCGCGGGTCATTACCACCTTACTCTGCTTCTCTTCCGGCGCGCCg                               <  2:338322/148‑1 (MQ=255)
                                                                                                                    tGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGTTTATCTTCGTCGCGCGGGTCATTACCACCTTACTCTGCTTCTCTTCCg                                       <  6:248391/138‑1 (MQ=255)
                                                                                                                    tGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGTTTATCTTCGTCGCGCGGGTCATTACCACCTTACTCTGCTTCTCTTCCg                                       >  5:248391/1‑138 (MQ=255)
                                                                                                                    tGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGTTTATCTTCGACGCGCGGGTCATTACCACCTTACTCTGCTTCTCTTCCGGCGCGCCGGGc                            >  2:297829/1‑149 (MQ=255)
                                                                                                                      gggTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGTTTATCTTCGTCGCGCGGGTCATTACCACCTTACTCTGCTTCTCTTCCGGCGCGCCGGGCgg                          >  6:136215/1‑149 (MQ=255)
                                                                                                                             tgGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGTTTATCTTCGTCGCGCGGGTCATTACCACCTTACTCTGCTTCTCTTCCGGCGCGCCGGGCGGTAttttt                   <  6:199587/149‑1 (MQ=255)
                                                                                                                               gCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGTTTATCTTCGTCGCGCGGGTCATTACCACCTTACTCTGCTTCTCTTCCGGCGCGCCGGGCGGTATTTTTGc                 >  6:27318/1‑149 (MQ=255)
                                                                                                                               gCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGTTTATCTTCGTCGCGCGGGTCATTACCACCTTACTCTGCTTCTCTTCCGGCGCGCCGGGCGGTATTTTTGc                 >  6:119604/1‑149 (MQ=255)
                                                                                                                                cACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGTTTATCTTCGTCGCGCGGGTCATTACCACCTTACTCTGCTTCTCTTCCGGCGCGCCGGGCGGTATTTTTGcc                >  5:113689/1‑149 (MQ=255)
                                                                                                                                 aCCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGTTTATCTTCGTCGCGCGGGTCATTACCACCTTACTCTGCTTCTCTTCCGGCGCGCCGGGCGGTATTTTTGccc               >  7:231262/1‑149 (MQ=255)
                                                                                                                                   cAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGTTTATCTTCGTCGCGCGGGTCATTACCACCTTACTCTGCTTCTCTTCCGGCGCGCCGGGCGGTATTTTTGcccc              >  5:36785/1‑148 (MQ=255)
                                                                                                                                   cAGCAACGTCGGGCGGCGGTTTTAACCTGATTCATATCGCTACCGCGGGGAATTTCAGCAAGGGAATGCTGGTGTTTATCTTCGTCGCGCGGGTCATTACCACCTTACTCTGCTTCTCTTCCGGCGCGCCGGGCGGTATTTTTGcccc              >  6:185653/1‑148 (MQ=255)
                                                                                                                                     gCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGTTTATCTTCGTCGCGCGGGTCATTACCACCTTACTCTGCTTCTCTTCCGGCGCGCCGGGCGGTATTTTTGCCCCGAt           >  3:124136/1‑149 (MQ=255)
                                                                                                                                        aCGTCGGGCGGCGGTTGTAACCTGATTCCTATCGCTAACGCGGGGAATTTCAGCATTGGAATGCTGGTGTTTATCTTCGTCGCGCGGGTCATTACCACCTTCCTCTGCTTCTCTTCCGGCGCGCCGGGCGGTATTTTTGCCCCGATGCt        <  8:241991/149‑1 (MQ=255)
                                                                                                                                         cGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGTTTATCTTCGTCGCGCGGGTCATTACCACCTTACTCTGCTTCTCTTCCGGCGCGCCGGGCGGTATTTTTGCCCCGATGCTg       <  5:344290/149‑1 (MQ=255)
                                                                                                                                          gTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGTTTATCTTCGTCGCGCGGGTCATTACCACCTTACTCTGCTTCTCTTCCGGCGCGCCGGGCGGTATTTTTGCCCCGATGCTgg      <  7:217651/149‑1 (MQ=255)
                                                                                                                                             gggCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGTTTATCTTCGTCGCGCGGGTCATTACCACCTTACTCTGCTTCTCTTCCGGCGCGCCGGGCGGTATTTTTGCCCCGATGCTGgcgc   >  4:184142/1‑149 (MQ=255)
                                                                                                                                              ggcggcGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGTTTATCTTCGTCGCGCGGGTCATTACCACCTTACTCTGCTTCTCTTCCGGCGCGCCGGGCGGTATTTTTGCCCCGATGCTGGCGCt  <  7:139067/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
CCCTATTTTTAATAAATGGGTGCTGGGGATGCAGGATTTGCTGCACCGTGTGCACGGCGGCAATATTACCAAATGGGTGCTAATGGGCGGTGCGATTGGCGGTCTGTGTGGATTGCTGGGGTTTGTGGCACCAGCAACGTCGGGCGGCGGTTTTAACCTGATTCCTATCGCTACCGCGGGGAATTTCAGCATGGGAATGCTGGTGTTTATCTTCGTCGCGCGGGTCATTACCACCTTACTCTGCTTCTCTTCCGGCGCGCCGGGCGGTATTTTTGCCCCGATGCTGGCGCT  >  NC_000913/175904‑176194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑