Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,174,843 A→G 5.9% L108P (CTG→CCG)  ycfT ← inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,174,8430AG 5.9% 206.1 / 3.2 70L108P (CTG→CCG) ycfTinner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (29/35);  new base G (2/2);  total (32/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01

CACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAA  >  NC_000913/1174701‑1174981
                                                                                                                                              |                                                                                                                                          
cACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCGGCCACTGGTTCAGCGCAc                                                                                                                                      >  6:248129/1‑149 (MQ=255)
  cGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACt                                                                                                                                     >  7:173800/1‑148 (MQ=255)
         gACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACgcca                                                                                                                             <  5:109047/149‑1 (MQ=255)
            aaTTAACGCATACAGATACCACAAGCTGGTGGTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATAGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTAAGCGCACTTAACgccagcc                                                                                                                          >  8:252773/1‑149 (MQ=255)
            aaTTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACgccagc                                                                                                                           >  3:144612/1‑148 (MQ=255)
             aTTAACGCATACAGATACCACAAGCTTGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACgccagcca                                                                                                                         <  3:231604/149‑1 (MQ=255)
                 aCGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCAGTGGAGGCATTGCTTAACTCGCGCTCAGGTGCCAGCCACTGGTTCCGCGCCCTTAACGCCAGCCACTGc                                                                                                                     >  8:325942/1‑149 (MQ=255)
                     aTACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGcacca                                                                                                                 <  4:222257/149‑1 (MQ=255)
                     aTACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGcacca                                                                                                                 >  8:235056/1‑149 (MQ=255)
                      tACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGcaccac                                                                                                                >  7:56968/1‑149 (MQ=255)
                         aGATACCACAAGCTGGTGCTGGCGGTGATCATCCTGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGcc                                                                                                             >  6:117662/1‑149 (MQ=255)
                           aTACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGccc                                                                                                            >  6:248757/1‑148 (MQ=255)
                              ccACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCaaa                                                                                                        <  7:125668/149‑1 (MQ=255)
                              ccACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCaaa                                                                                                        <  5:106224/149‑1 (MQ=255)
                               cacaAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAg                                                                                                       >  7:338168/1‑149 (MQ=255)
                                acaAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAgc                                                                                                      <  3:124225/149‑1 (MQ=255)
                                     cTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAg                                                                                                 <  3:15105/149‑1 (MQ=255)
                                        gTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCAc                                                                                              >  7:133834/1‑149 (MQ=255)
                                            tGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCAcc                                                                                             >  8:275294/1‑146 (MQ=255)
                                              gCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCCGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCagaa                                                                                        <  5:248129/149‑1 (MQ=255)
                                                ggTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCagaaga                                                                                      <  7:275294/149‑1 (MQ=255)
                                                  tGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATg                                                                                    <  5:111095/149‑1 (MQ=255)
                                                    atcatcCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGtt                                                                                  >  5:149029/1‑149 (MQ=255)
                                                     tcatcCCGTGGAGGAACTCACCGGTGGAATCTGCATATGCTGCATTGGAGGCATTTCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCTGCGCACTTAACGGCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTc                                                                                 <  3:106462/149‑1 (MQ=255)
                                                      catcCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCACAGCGCCAGCCCCCAGAAGATGTTc                                                                                 >  6:87009/1‑148 (MQ=255)
                                                       atcCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCGGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTc                                                                                 >  6:196733/1‑147 (MQ=255)
                                                       atcCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCGGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTc                                                                                 >  6:192051/1‑147 (MQ=255)
                                                       atcCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTcc                                                                                <  3:48041/148‑1 (MQ=255)
                                                        tcCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTc                                                                                 >  4:137058/1‑146 (MQ=255)
                                                               cAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCGGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGt                                                                        <  5:192051/148‑1 (MQ=255)
                                                               cAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCGGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGt                                                                        <  5:196733/148‑1 (MQ=255)
                                                                aGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGttt                                                                      <  8:133834/149‑1 (MQ=255)
                                                                   aaCTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATc                                                                   <  8:173800/149‑1 (MQ=255)
                                                                            gttgAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAAACGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAAtt                                                          <  3:290516/146‑1 (MQ=255)
                                                                             tGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAAttt                                                         <  7:23592/149‑1 (MQ=255)
                                                                               gAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTcc                                                       <  6:182533/149‑1 (MQ=255)
                                                                               gAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTc                                                        <  4:119193/148‑1 (MQ=255)
                                                                               gAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTc                                                        <  5:248757/148‑1 (MQ=255)
                                                                                aaTCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTccc                                                      >  2:352752/1‑149 (MQ=255)
                                                                                 aTCGGCATAAGCGGCATTGGAGTCATTGCTTAAATCGCGCACAGGTGCCAGCCACTTGTTCAGCGCACTTAACGCCAGACACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATACGTTTATCGAGACAATTTccca                                                     >  4:257377/1‑148 (MQ=255)
                                                                                     gCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAgcgc                                                                                                    <  5:60478/98‑1 (MQ=255)
                                                                                     gCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTCCACAACGCCCCAAAgaga                                                                                                    >  6:60478/1‑95 (MQ=255)
                                                                                        tAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCCCGCCCCAAAGCGCCAGCACCCAGAAGATGATCCAGATCCGTTTAACGAGACAATTTCCCCACGGTAc                                              >  5:75650/1‑149 (MQ=255)
                                                                                            cGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTg                                          <  7:146654/149‑1 (MQ=255)
                                                                                                   ggAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGata                                    <  3:137058/148‑1 (MQ=255)
                                                                                                    gAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGAtata                                  <  5:117662/149‑1 (MQ=255)
                                                                                                       gCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAgcg                               >  8:12766/1‑149 (MQ=255)
                                                                                                          ttGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCCCCACGCCCCAAAGCGCCAGCCCCCAGAAGATGTTCCAGATCCGTTGATCGATCCAATTTCCCCACGGTACGCTGTCGATATAgcggc                             >  6:294757/1‑148 (MQ=255)
                                                                                                          ttGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAgcggc                             >  6:75688/1‑148 (MQ=255)
                                                                                                            gCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTc                                                        <  1:110502/119‑1 (MQ=255)
                                                                                                            gCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTc                                                        >  2:110502/1‑119 (MQ=255)
                                                                                                             cTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCg                                       >  5:256790/1‑135 (MQ=255)
                                                                                                             cTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCg                                       <  6:256790/135‑1 (MQ=255)
                                                                                                              ttAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGTCACTGCACCACGCCCCAACGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTCCGCTGTCGATATAGCGGCGAAt                         >  6:284899/1‑148 (MQ=255)
                                                                                                              ttAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAAt                         >  5:230340/1‑148 (MQ=255)
                                                                                                                  aTCGCGCTCAGGTGCCAGCCTCTGGTTCAGGGCACTTAAGGCCATCCACTGCACAAAGCCCCTAAGCGCGAGCACCCAGAAGATGTTCTAGATCCGTTTATAGTGACAATTTCCCCACGGTACGCTGTCGATATAGGGGCGAATCaaa                     <  6:80495/148‑1 (MQ=255)
                                                                                                                   tCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCaaa                     >  4:167582/1‑147 (MQ=255)
                                                                                                                   tCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATa                   <  5:75688/149‑1 (MQ=255)
                                                                                                                           aGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCTAATAGCCGGat            <  7:12766/148‑1 (MQ=255)
                                                                                                                            ggTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGatat          <  3:167582/149‑1 (MQ=255)
                                                                                                                            ggTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGatat          <  3:144665/149‑1 (MQ=255)
                                                                                                                             gTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGAtata         <  8:144859/149‑1 (MQ=255)
                                                                                                                              tGCCAGCCACTGGTGCAGCGCACTTAACGCCATCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATaa        <  3:252971/149‑1 (MQ=255)
                                                                                                                               gccagccaCTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGAtata         >  7:93432/1‑147 (MQ=255)
                                                                                                                                ccagccaCTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAAt                    <  1:287318/135‑1 (MQ=255)
                                                                                                                                ccagccaCTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATaaa       <  4:306416/148‑1 (MQ=255)
                                                                                                                                ccagccaCTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGACCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAg      <  1:90600/149‑1 (MQ=255)
                                                                                                                                caagcaaCTGGTTAAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCACAACGGAACGCTCTCGATATAGAGGCGAATCAAAt                    >  2:287318/3‑135 (MQ=255)
                                                                                                                                   gccaCTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCCGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGGCGATATAGCGGCGAATCAAATAGCCGGATATAAAGaa    >  2:272866/1‑148 (MQ=255)
                                                                                                                                    ccaCTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGaaaa  <  1:352752/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                          
CACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGCCAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAATTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAA  >  NC_000913/1174701‑1174981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑