Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,272,763 C→T 5.3% R370H (CGT→CAT)  uvrA ← ATPase and DNA damage recognition protein of nucleotide excision repair excinuclease UvrABC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,272,7630CT 5.3% 244.3 / 2.4 75R370H (CGT→CAT) uvrAATPase and DNA damage recognition protein of nucleotide excision repair excinuclease UvrABC
Reads supporting (aligned to +/- strand):  ref base C (36/35);  new base T (2/2);  total (38/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01

GCGCGCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTACAACACCACTTTATGCACGTTCGCGCTCAGGCTGCCCCACGGCGCTTCGACGTCGAACTTATAGTGATCTGCCAGCGATTTCA  >  NC_000913/4272615‑4272910
                                                                                                                                                    |                                                                                                                                                   
gcgcgcTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGCACGGATgacgac                                                                                                                                                     >  1:358242/1‑149 (MQ=255)
gcgcgcTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATgacgac                                                                                                                                                     <  5:307464/149‑1 (MQ=255)
    gcTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACTGATGACGACGAAt                                                                                                                                                 <  7:128430/149‑1 (MQ=255)
        cccGACGCAGACGCGTCCCTTCGCAGCTGGCGCAAGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATgg                                                                                                                                               <  5:332696/147‑1 (MQ=255)
         ccGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAg                                                                                                                                             <  1:299481/148‑1 (MQ=255)
         ccGACGCAGACGCGTCCCTTCGCAGCTGGAGCACGGACGATTCCTGATAAACTTGGCTAATTCTTCGCGTACCGCGCGGGATTCCGTCTCTTTATAGCGGCTCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAg                                                                                                                                             <  4:35941/148‑1 (MQ=255)
          cGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAgg                                                                                                                                            >  6:151905/1‑148 (MQ=255)
           gACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGATGAATGGAg                                                                                                                                             <  5:64046/146‑1 (MQ=255)
           gACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGATGAATGGAg                                                                                                                                             >  4:331367/1‑146 (MQ=255)
           gACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGATGAATGGAg                                                                                                                                             <  3:331367/146‑1 (MQ=255)
           gACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGATGAATGGAg                                                                                                                                             >  6:64046/1‑146 (MQ=255)
              gCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCg                                                                                                                                       >  8:93777/1‑149 (MQ=255)
              gCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCg                                                                                                                                       >  3:93791/1‑149 (MQ=255)
                aGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGc                                                                                                                                      >  4:7199/1‑148 (MQ=255)
                 gACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTCTGCAGCACGCCTTCGCAGGGATGACGCCGAATGGAGGTTTCGCCa                                                                                                                                    >  4:195780/1‑149 (MQ=255)
                 gACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCa                                                                                                                                    <  8:343107/149‑1 (MQ=255)
                  aCGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCAc                                                                                                                                   <  8:110991/149‑1 (MQ=255)
                      gTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTGTTTATAGCGGCGCTCCAGATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATc                                                                                                                               >  5:13110/1‑149 (MQ=255)
                      gTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATc                                                                                                                               >  1:261808/1‑149 (MQ=255)
                       tCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCg                                                                                                                              >  2:297517/1‑149 (MQ=255)
                          cTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGtt                                                                                                                            >  2:245078/1‑148 (MQ=255)
                           ttCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCa                                                                                                                          >  4:218994/1‑149 (MQ=255)
                             cggaGCTGGCGCAGGGACGTTTACTGATAAACTTGGCTAATTCTTCTAGTAGCGAGCTGGATTCCGTCTCTTTATTGCGGCGGTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGTTCGTTCAt                                                                                                                         <  6:252011/145‑1 (MQ=255)
                             cGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCAt                                                                                                                         <  2:3378/148‑1 (MQ=255)
                                  cTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTAttt                                                                                                                   <  5:24254/149‑1 (MQ=255)
                                       gcACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAAtt                                                                                                              <  2:230126/149‑1 (MQ=255)
                                          cGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCaa                                                                                                           <  5:184014/149‑1 (MQ=255)
                                           ggACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAAt                                                                                                          >  2:196510/1‑149 (MQ=255)
                                           ggACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAAt                                                                                                          <  4:93791/149‑1 (MQ=255)
                                             aCGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGt                                                                                                        >  2:95956/1‑149 (MQ=255)
                                                 ttACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACTGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTc                                                                                                    <  3:229689/149‑1 (MQ=255)
                                                   aCTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCtt                                                                                                  >  4:48581/1‑149 (MQ=255)
                                                    cTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCttt                                                                                                 <  5:334224/149‑1 (MQ=255)
                                                    cTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCttt                                                                                                 <  5:276687/149‑1 (MQ=255)
                                                    cTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCttt                                                                                                 <  5:308042/149‑1 (MQ=255)
                                                    cTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCttt                                                                                                 <  2:261808/149‑1 (MQ=255)
                                                    cTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCtt                                                                                                  <  3:218994/148‑1 (MQ=255)
                                                      gATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTAttt                                                                                                                   >  3:325075/1‑129 (MQ=255)
                                                      gATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTAttt                                                                                                                   <  4:325075/129‑1 (MQ=255)
                                                      gATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGCTATCGCCACGATCGTTCATGTATTTGAATTCAATGATTTCTTTGc                                                                                               >  6:277832/1‑149 (MQ=255)
                                                       aTAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGcc                                                                                              <  2:256724/149‑1 (MQ=255)
                                                       aTAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGGTCATGTATTTGAATTCAATGTTTTCTTTGc                                                                                               >  4:16547/1‑148 (MQ=255)
                                                                 cTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTc                                                                                                    <  4:244778/133‑1 (MQ=255)
                                                                 cTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTc                                                                                                    >  3:244778/1‑133 (MQ=255)
                                                                  tAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGCCGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTac                                                                                    >  7:98712/1‑148 (MQ=255)
                                                                    aTTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTacaa                                                                                  <  3:7199/148‑1 (MQ=255)
                                                                     ttcttcGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTacaaca                                                                                <  3:261248/149‑1 (MQ=255)
                                                                        ttcGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTACAAcacca                                                                             >  2:211863/1‑149 (MQ=255)
                                                                             gTACCGCGATGCATACCGTCTATTTATAGCGGCGATCCATATTATTCAGCACGACATCCAACGCATGACGACGAATGGAGGTAACGCCACGATCGTTAAAGTATTTGAATTCAATGTTATCTTTGCCAGAACCGTACATCACAACTTTa                                                                        >  4:109670/1‑149 (MQ=255)
                                                                              tACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTACAACACCACTTTAt                                                                       <  2:153571/149‑1 (MQ=255)
                                                                                ccGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTACAACACCACTTTATg                                                                      <  1:297517/148‑1 (MQ=255)
                                                                                ccGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTACAACACCACTTTATg                                                                      <  1:245078/148‑1 (MQ=255)
                                                                                 cgcgCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTACAACACCACTTTATGCa                                                                    <  3:162874/149‑1 (MQ=255)
                                                                                  gcgcTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTACAACACCACTTTATGCAc                                                                   <  5:81721/149‑1 (MQ=255)
                                                                                    gcTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACTGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTACAACACCACTTTATGCACGt                                                                 <  1:198431/149‑1 (MQ=255)
                                                                                       ggATGCAGTCTCTTTTTAGGGGCGGTCCTTATTATGCTGGAGGCGGTGGTTGGGATGACGACGAATGGAGGTAACGCCACGATCGTTCATGTATTTGAATTCAATCTTTTCTTTGCCAGAACCGTACAACACCACTTTATGCACGTTcg                                                              <  6:107654/149‑1 (MQ=255)
                                                                                              gTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTACAACACCACTTTATGCACGTTCGCGCTCAg                                                       >  5:31849/1‑149 (MQ=255)
                                                                                                          cggcgCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCAATCTCGTTCATGTATTTCAATTAAATGTATAATTTGCCAGAACAGTACAACACCACTTTATGCACGTTCCCCCTCAGGCTCCCCCACgg                                           >  5:83399/1‑149 (MQ=255)
                                                                                                               cTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTACAACACCACTTTATGCACGTTCGCGCTCAGGCTGCCCCCCGGCGCtt                                      >  5:61409/1‑149 (MQ=255)
                                                                                                                tCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTACAACACCACTTTATGCACGTTCGCGCTCAGGCTGCCCCACGGCGCTTc                                     >  8:71521/1‑149 (MQ=255)
                                                                                                                 ccATATTATGCAGCACGCCTTCGAACGGATGCCGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTACAACACCACTTTATGCACGTTCGCGCTCAGGCTGCCCCACGGCGCTTCg                                    >  8:77961/1‑149 (MQ=255)
                                                                                                                 ccATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTACAACACCACTTTATGCACGTTCGCGCTCAGGCTGCCCCACGGCGCTTc                                     >  8:55120/1‑148 (MQ=255)
                                                                                                                  cATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTACAACACCACTTTATGCACGTTCGCGCTCAGGCTGCCCCACGGCGCTTc                                     <  7:165723/147‑1 (MQ=255)
                                                                                                                  cATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTACAACACCACTTTATGCACGTTCGCGCTCAGGCTGCCCCACGGCGCTTc                                     <  3:48581/147‑1 (MQ=255)
                                                                                                                   atatTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTACAACACCACTTTATGCACGTTCGCGCTCAGGCTGCCCCACGGCGCTTCGAc                                  >  5:216782/1‑149 (MQ=255)
                                                                                                                    tattatGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTACAACACCACTTTATGCACGTTCGCGCTCAGGCTGCCCCACGGCGCTTCGACg                                 >  2:90032/1‑149 (MQ=255)
                                                                                                                       tatGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTACAACACCACTTTATGCACGTTCGCGCTCAGGCTGCCCCACGGCGCTTCGACGTc                               >  6:148108/1‑148 (MQ=255)
                                                                                                                          gcagcaCGCCTTCGAACGGATGACGACGAATCGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCTTACAACACCACTTTATGCACGTTCGCGCTCAGTCTGCCCCACGGAGATTCGACGTCGAAc                           >  3:311453/1‑149 (MQ=255)
                                                                                                                           cagcaCGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTACAACACCACTTTATGCACGTTCGCGCTCAGGCTGCCCCACGGCGCTTCGACGTCGAAc                           <  1:90032/148‑1 (MQ=255)
                                                                                                                               aCGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTACAACACCACTTTATGCACGTTCGCGCTCAGGCTGCCCCACGGCGCTTCGACGTCGAACTtata                      <  7:93777/149‑1 (MQ=255)
                                                                                                                                 gCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTACAACACCACTTTATGCACGTTCGCGCTCAGGCTGCCCCACGGCGCTTCGACGTCGAACTTATAGt                    >  7:14530/1‑149 (MQ=255)
                                                                                                                                    ttCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTACAACACCACTTTATGCACGTTCGCGCTCAGGCTGCCTCACGGCGCTTCGACGTCGAACTTATAGTGAt                 >  5:150086/1‑149 (MQ=255)
                                                                                                                                    ttCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTACAACACCACTTTATGCACGTTCGCGCTCAGGCTGCCCCACGGCGCTTCGACGTCGAACTTATAGTGAt                 >  7:98458/1‑149 (MQ=255)
                                                                                                                                    ttCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTACAACACCACTTTATGCACGTTCGCGCTCAGGCTGCCCCACGGCGCTTCGACGTCGAACTTATAGTGAt                 >  1:194825/1‑149 (MQ=255)
                                                                                                                                      cGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTACAACACCACTTTATGCACGTTCGCGCTCAGGCTGCCCCACGGCGCTTCGACGTCGAACTTATAGTGAt                 <  1:95956/147‑1 (MQ=255)
                                                                                                                                       gAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTACAACACCACTTTATGCACGTTCGCGCTCAGGCTGCCCCACGGCGCTTCGACGGCGAACTTATAGTGAGCTg              >  2:267658/1‑149 (MQ=255)
                                                                                                                                            gATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTACAACACCACTTTATGCACGTTCGCGCTCAGGCTGCCCCACGGCGCTTCGACGTCGAACTTATAGTGATCTGCCAGc         >  6:187250/1‑149 (MQ=255)
                                                                                                                                                  gacgaATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTACAACACCACTTTATGCACGTTCGCGCTCAGGCTGCCCCACGGCGCTTCGACGTCGAACTTATAGTGATCTGCCAGCGAttt    >  7:137771/1‑148 (MQ=255)
                                                                                                                                                  gacgaATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTACAACACCACTTTATGCACGTTCGCGCTCAGGCTGCCCCACGGCGCTTCGACGTCGAACTTATAGTGATCTGCCAGCGAttt    >  5:174180/1‑148 (MQ=255)
                                                                                                                                                  gacgaATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTACAACACCACTTTATGCACGTTCGCGCTCAGGCTGCCCCACGGCGCTTCGACGTCGAACTTATAGTGATCTGCCAGCGATTTc   <  8:98458/149‑1 (MQ=255)
                                                                                                                                                  gacgaATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTACAACACCACTTTATGCACGTTCGCGCTCAGGCTGCCCAACGGCGCTTCGACGTCGAACTTATAGTGATCTGCCAGCGAttt    >  3:279076/1‑148 (MQ=255)
                                                                                                                                                   acgaATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTACAACACCACTTTATGCACGTTCGCGCTCAGGCTGCCCCACGGCGCTTCGACGTCGAACTTATAGTGATCTGCCAGCGATTTCa  <  6:13110/149‑1 (MQ=255)
                                                                                                                                                   acgaATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTACAACACCACTTTATGCACGTTCGCGCTCAGGCTGCCCCACGGCGCTTCGACGTCGAACTTATAGTGATCTGCCAGCGATTTCa  <  5:148108/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCGCGCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTCGAACGGATGACGACGAATGGAGGTATCGCCACGATCGTTCATGTATTTGAATTCAATGTTTTCTTTGCCAGAACCGTACAACACCACTTTATGCACGTTCGCGCTCAGGCTGCCCCACGGCGCTTCGACGTCGAACTTATAGTGATCTGCCAGCGATTTCA  >  NC_000913/4272615‑4272910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑