Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,806,319	C→G	intergenic (‑236/‑51)	ydiY ← / → pfkB	acid‑inducible putative outer membrane protein/6‑phosphofructokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,806,319	0	C	G	100.0%	129.5 / NA	37	intergenic (‑236/‑51)	ydiY/pfkB	acid‑inducible putative outer membrane protein/6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (18/19);  total (18/19)

GGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCG  >  NC_000913/1806186‑1806463                                                                                                                                      |                                                                                                                                                 ggCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTc                                                                                                                                   >  6:59836/1‑149 (MQ=255)    gcgAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGt                                                                                                                                >  8:13422/1‑149 (MQ=255)    gcgAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGt                                                                                                                                <  5:20521/149‑1 (MQ=255)      gAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGc                                                                                                                              >  5:90870/1‑149 (MQ=255)            cTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACt                                                                                                                        <  1:36122/149‑1 (MQ=255)                 cAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAAt                                                                                                                   >  2:137345/1‑149 (MQ=255)                        ttCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAACTCAGCCta                                                                                                            <  7:13422/149‑1 (MQ=255)                              tttCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATaggag                                                                                                      <  1:137345/149‑1 (MQ=255)                               ttCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATagga                                                                                                       <  6:162149/147‑1 (MQ=255)                               ttCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAAGAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATagga                                                                                                       >  5:162149/1‑147 (MQ=255)                                tCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACttt                                                                                                                                             <  6:8242/108‑1 (MQ=255)                                tCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACttt                                                                                                                                             >  5:8242/1‑108 (MQ=255)                                tCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATaggagg                                                                                                     >  7:135998/1‑148 (MQ=255)                                      tttGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGc                                                                                                               >  5:100822/1‑132 (MQ=255)                                      tttGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGc                                                                                                               <  6:100822/132‑1 (MQ=255)                                        tGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATgg                                                                                            >  7:32730/1‑149 (MQ=255)                                                      gCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACg                                                                              >  2:106256/1‑149 (MQ=255)                                                       cGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGt                                                                             >  5:173660/1‑149 (MQ=255)                                                          aaaCAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGa                                                                          <  1:106256/149‑1 (MQ=255)                                                             cAGATTTTTATTAATATATACTTATCTGCAAAATTTTAAATAAAGCACCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGACTCGGCGCCAGACTGCAAGCAGCCTATAGGAGGAAATGATGGGACGTATCTATACGTTGacac                                                                       <  4:15331/149‑1 (MQ=255)                                                                     tatttatATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGcc                                                                <  5:59836/148‑1 (MQ=255)                                                                          atatatatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCctctc                                                           <  8:32730/148‑1 (MQ=255)                                                                          atatatatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCctctc                                                           <  6:90870/148‑1 (MQ=255)                                                                           tatatatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCctctct                                                          <  8:135998/148‑1 (MQ=255)                                                                              atatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCa                                                                                                                 <  4:125896/90‑1 (MQ=255)                                                                              atatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCa                                                                                                                 >  3:125896/1‑90 (MQ=255)                                                                                     tCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACtt                                                                     >  8:60039/1‑127 (MQ=255)                                                                                     tCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACtt                                                                     <  7:60039/127‑1 (MQ=255)                                                                                            aaTTTTAAATTAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTAc                                        >  6:92304/1‑149 (MQ=255)                                                                                               tttAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTAcccc                                     >  1:48140/1‑149 (MQ=255)                                                                                                   aataaaGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCa                                  >  5:173088/1‑148 (MQ=255)                                                                                                    ataaaGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCaaa                                <  5:4115/149‑1 (MQ=255)                                                                                                    ataaaGCTCCAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCaaa                                >  1:168865/1‑149 (MQ=255)                                                                                                            ccAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATc                          >  3:89364/1‑147 (MQ=255)                                                                                                            ccAATAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATc                          <  4:89364/147‑1 (MQ=255)                                                                                                               aTAAATCATATTGTTAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGaa                     <  5:92304/149‑1 (MQ=255)                                                                                                                            ttAATTTCTTGACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTgcgc         <  6:173660/148‑1 (MQ=255)                                                                                                                                  tcttgACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCg  >  2:67979/1‑149 (MQ=255)                                                                                                                                      |                                                                                                                                                 GGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCG  >  NC_000913/1806186‑1806463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑