Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,603,934 C→T 7.0% R306C (CGC→TGC)  lsrC → autoinducer 2 import system permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,603,9340CT 7.0% 172.7 / 3.3 58R306C (CGC→TGC) lsrCautoinducer 2 import system permease protein
Reads supporting (aligned to +/- strand):  ref base C (28/25);  new base T (2/2);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.39e-01

GTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAACGGGAGGCCGCATAATGCGTATTCGCTACGGTTGGGAACTGGCTCTTGCC  >  NC_000913/1603788‑1604082
                                                                                                                                                  |                                                                                                                                                    
gtggtTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGc                                                                                                                                                    >  8:307627/1‑149 (MQ=255)
       cGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGtt                                                                                                                                             <  8:132299/149‑1 (MQ=255)
       cGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGtt                                                                                                                                             <  3:165665/149‑1 (MQ=255)
       cGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGt                                                                                                                                              <  5:269127/148‑1 (MQ=255)
         gTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTtgt                                                                                                                                           <  5:23977/149‑1 (MQ=255)
            cGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGATGCCTGCGTTGTgcg                                                                                                                                        >  5:199209/1‑149 (MQ=255)
            cGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGATGCCTGCGTTGTgcg                                                                                                                                        >  7:104202/1‑149 (MQ=255)
              aTCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGtt                                                                                                                                             <  4:271153/142‑1 (MQ=255)
              aTCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGtt                                                                                                                                             >  3:271153/1‑142 (MQ=255)
              aTCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTATGATGGACGCATGCGTTGTGCGCt                                                                                                                                      >  7:188676/1‑149 (MQ=255)
                 aTTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTgg                                                                                                                                    >  1:264050/1‑148 (MQ=255)
                   tGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGGGCGGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGGGCTGGGGTTTGATGGACGCCTGCGTTGTGCGCt                                                                                                                                      <  1:373702/144‑1 (MQ=255)
                           tACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTa                                                                                                                         >  8:223281/1‑149 (MQ=255)
                             cTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTAcg                                                                                                                       <  7:307627/149‑1 (MQ=255)
                                ggCGCCTGGTTCCTGACGCAGATCGCTAGCGTACTGGTGCGGTTGCGCCTTCCGGCATGGGGGAAAGAGTTTCTCGCTGGGCTGGCGCTGCTGGCGGTGCTGGTGTTTGATGGCCGCCTGCGTTGTGCGCTGGAACGTAATCTAcgg                                                                                                                      <  8:160866/147‑1 (MQ=255)
                                 gcgcATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGGTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGGGCTGGTGGGTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTAcggcgg                                                                                                                   <  2:317806/149‑1 (MQ=255)
                                      tGGTTCCTGACGCAGATAGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCaaa                                                                                                               >  4:265125/1‑148 (MQ=255)
                                            cTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGc                                                                                                        <  7:111534/149‑1 (MQ=255)
                                             tGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTATGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTCCGGCGGCAAAAATATGcc                                                                                                       >  4:321048/1‑149 (MQ=255)
                                                 gCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGc                                                                                                        >  3:248597/1‑144 (MQ=255)
                                                        gATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGAc                                                                                            <  2:264050/149‑1 (MQ=255)
                                                              gTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGc                                                                                                                                                    >  7:176618/1‑87 (MQ=255)
                                                              gTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGc                                                                                                                                                    <  8:176618/87‑1 (MQ=255)
                                                              gTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCAcc                                                                                      >  2:312613/1‑149 (MQ=255)
                                                                aCTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGc                                                                                    >  2:320865/1‑149 (MQ=255)
                                                                  tGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTgcgc                                                                                                                                       <  5:233119/96‑1 (MQ=255)
                                                                  tGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCa                                                                                  >  2:146212/1‑149 (MQ=255)
                                                                  tGGTGCTGTTGCGCATTACGGCATGGTGGAATGATTTTAACGCGGGTATGGTTCTGCTGGCGGTGCTGGTGATTGATGGACGCATGCGTAGTgcgc                                                                                                                                       >  6:233119/1‑96 (MQ=255)
                                                                     tGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATcc                                                                               >  4:370412/1‑149 (MQ=255)
                                                                          ttGCGCATTCCCGCATGGTGCAATGATTTTATCCCGGGTCTGGTTCTGCTGCCGGTGCTGGTGATTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTa                                                                           >  2:264431/1‑148 (MQ=255)
                                                                            gcgcATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTaaa                                                                         <  7:223281/148‑1 (MQ=255)
                                                                            gcgcATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAAc                                                                        >  3:18754/1‑149 (MQ=255)
                                                                              gcATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAAccc                                                                      >  7:226297/1‑149 (MQ=255)
                                                                                    cGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTAcgg                                                                                                                      >  8:225230/1‑95 (MQ=255)
                                                                                    cGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTAcgg                                                                                                                      <  7:225230/95‑1 (MQ=255)
                                                                                              gAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTaaa                                                       >  2:375510/1‑148 (MQ=255)
                                                                                                   aTTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCTCTTTATGAAGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAACgg                                                 <  3:265125/149‑1 (MQ=255)
                                                                                                      ttATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAACGGGa                                               <  1:312613/148‑1 (MQ=255)
                                                                                                        aTCGCGGGGCTGGTTCTGCTGGCGGTGCTGGTCTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAACGGGAgg                                             <  6:154236/148‑1 (MQ=255)
                                                                                                             gggTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGc                                                                                                    <  2:173754/88‑1 (MQ=255)
                                                                                                                tCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAACGGGAGGCCGCATAAt                                    >  3:87269/1‑149 (MQ=255)
                                                                                                                  gtggtCTGCTGGCGGTTCTGGGTGTTGATGGACCCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAACGGGAGGCCGCATAATGc                                  <  4:219934/145‑1 (MQ=255)
                                                                                                                     ttCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGTCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAACGGGAGGCCGCATAATGCGTa                               <  4:345303/149‑1 (MQ=255)
                                                                                                                        ggctgGCGGGGCTGGTGTTTGATGGAGGGCTGCGTTGTGCGCTGGAACGTAATCTAGGGCTGCAAATATATGCCCGCGTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCTGGTAAAAAACGGGAGGCCTCATAATGCGTAtt                             <  8:188676/147‑1 (MQ=255)
                                                                                                                         gctgGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAACGGGAGGCCGCATAATGCGTATTCg                           >  2:226234/1‑149 (MQ=255)
                                                                                                                             gCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAACGGGAGGCCGCATAATGCGTATTCGATAc                       >  3:310726/1‑149 (MQ=255)
                                                                                                                                 tGCTGGTGTTTGATGGATGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAACGGGAGGCCGCATAATGCGTATTCGCTACGGtt                   <  8:104202/149‑1 (MQ=255)
                                                                                                                                 tGCTGGTGTTTGATGGATGCCTGCGTTGTGCGCTGGAACGGAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAACGGGAGGCCGCATAATGCGTATTCGCTACGGtt                   <  6:199209/149‑1 (MQ=255)
                                                                                                                                 tGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAACGGGAGGCCGCATAATGc                                  <  6:381144/134‑1 (MQ=255)
                                                                                                                                 tGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAACGGGAGGCCGCATAATGc                                  >  5:381144/1‑134 (MQ=255)
                                                                                                                                         tttGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAACGGGAGGCCGCATAATGCGTATTCGCTACGGTTGGGAACt            <  1:226234/148‑1 (MQ=255)
                                                                                                                                         tttGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAACGGGAGGCCGCATAATGCGTATTCGCTACGGTTGGGAACTg           >  8:184919/1‑149 (MQ=255)
                                                                                                                                            gATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAACGGGAGGCCGCATAATGCGTATTCGCTACGGTTGGGAACTGGct        >  2:67341/1‑149 (MQ=255)
                                                                                                                                            gATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAACGGGAGGCCGCATAATGCGTATTCGCTACGGTTGGGAACTGGct        >  7:145782/1‑149 (MQ=255)
                                                                                                                                              tGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAACGGGAGGCCGCATAATGCGTATTCGCTACGGTTggg                >  6:220471/1‑139 (MQ=255)
                                                                                                                                              tGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAACGGGAGGCCGCATAATGCGTATTCGCTACGGTTggg                <  5:220471/139‑1 (MQ=255)
                                                                                                                                               ggACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAACGGGAGGCCGCATAATGCGTATTCGCTACGGTTGGGAACTGGCTCtt     >  6:259849/1‑149 (MQ=255)
                                                                                                                                                 aCGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCTCCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAACGTGAGGCCGCATAATGCGTATTCGCTACGGTTGGGAACTGGCTCTTGc   <  8:226297/149‑1 (MQ=255)
                                                                                                                                                  cGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAACGGGAGGCCGCATAATGCGTATTCGCTACGGTTGGGAACTGGCTCTTGcc  >  8:383116/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTTGCGCATTCCGGCATGGTGGAATGATTTTATCGCGGGTCTGGTTCTGCTGGCGGTGCTGGTGTTTGATGGACGCCTGCGTTGTGCGCTGGAACGTAATCTACGGCGGCAAAAATATGCCCGCTTTATGACGCCACCGCCATCCGTTAAACCCGCTTCGTCAGGTAAAAAACGGGAGGCCGCATAATGCGTATTCGCTACGGTTGGGAACTGGCTCTTGCC  >  NC_000913/1603788‑1604082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑