Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,567,467 G→T 5.5% intergenic (‑327/+37) dosC ← / ← yddW diguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator/liprotein, glycosyl hydrolase homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,567,4670GT 5.5% 347.4 / 5.1 100intergenic (‑327/+37)dosC/yddWdiguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator/liprotein, glycosyl hydrolase homolog
Reads supporting (aligned to +/- strand):  ref base G (46/48);  new base T (2/4);  total (48/52)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

TTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGT  >  NC_000913/1567322‑1567613
                                                                                                                                                 |                                                                                                                                                  
ttttCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTa                                                                                                                                                  >  2:395190/1‑148 (MQ=255)
 tttCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATAAATTTTTATTAGGGATTTATGGCTGTTTAACTTAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTTAGAGGGTAACAAGCCGGTGGGTa                                                                                                                                                  >  5:43216/1‑147 (MQ=255)
 tttCGGTGACAGCGAATATCGTATGGGTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTa                                                                                                                                                  >  5:37860/1‑147 (MQ=255)
         aCAGCGACTATCGTATGGGTTTTCATATTCCTACATTTTTATTAGGGCTTTCTGGCTGTTTAACTACGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGc                                                                                                                                        <  8:240949/149‑1 (MQ=255)
           aGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGCTTTACCCACCGGCtt                                                                                                                                      <  5:389572/149‑1 (MQ=255)
              gAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATATATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGtt                                                                                                                                   <  2:205196/149‑1 (MQ=255)
                   tCGTCTGGTTTTTCATATTCATACATTTTTATTAGGGAGTTATGGCTGGTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTCACAAGCCGGTGGGTAAAGCACCGGCTTGTTACaaa                                                                                                                              <  8:230809/149‑1 (MQ=255)
                   tCGTATGGTTTTTCATATTCCAACATTTTTAATAGGGAATTATGGCTGCTTAACTACGTGTGCTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACaaa                                                                                                                              <  6:43216/149‑1 (MQ=255)
                   tCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGTGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACaaa                                                                                                                              <  6:77117/149‑1 (MQ=255)
                   tCGTATGGTCTTTCCTATTCATACAATTTTATTAGGGAGTTATGGCTGTTTAACTAAGTGCGCTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACaaa                                                                                                                              <  6:37860/149‑1 (MQ=255)
                      tATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGt                                                                                                                            >  4:119191/1‑148 (MQ=255)
                          gTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGaa                                                                                                                       <  7:28039/149‑1 (MQ=255)
                          gTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGaa                                                                                                                       <  1:119953/149‑1 (MQ=255)
                           tttttCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCAc                                                                                                                                            <  6:358586/127‑1 (MQ=255)
                           tttttCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCAc                                                                                                                                            >  5:358586/1‑127 (MQ=255)
                             tttCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATgg                                                                                                                    >  1:172946/1‑149 (MQ=255)
                                cctcttcataCATTTTTATTAGGGATTTCTGGCTCTTTAACTAAGTGTGGCTAATTTGACTTAACTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAACGCACCGGCTTGTTACAAAGTAAGAATGGGa                                                                                                                  <  2:364097/144‑1 (MQ=255)
                                catattcataCATTTTTATTAGTGATTTATGGCTGTTTAACTAAGTGTGTTTAATTTGACTTAAGTAAGCATGATTATTAGTTGTATATTTTAAGAGTGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGa                                                                                                                  <  5:392203/148‑1 (MQ=255)
                                catattcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATgg                                                                                                                    >  6:142696/1‑146 (MQ=255)
                                catattcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATgg                                                                                                                    <  5:142696/146‑1 (MQ=255)
                                catattcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGa                                                                                                                  <  2:172946/148‑1 (MQ=255)
                                  tattcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGtt                                                                                                               >  8:89925/1‑149 (MQ=255)
                                  tattcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGtt                                                                                                               <  8:78018/149‑1 (MQ=255)
                                  tattcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGtt                                                                                                               <  3:119191/149‑1 (MQ=255)
                                   attcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGCTTTACCCACCGGCtt                                                                                                                                      >  5:296346/1‑125 (MQ=255)
                                   attcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGCTTTACCCACCGGCtt                                                                                                                                      <  6:296346/125‑1 (MQ=255)
                                    ttcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGttt                                                                                                              >  6:386588/1‑148 (MQ=255)
                                      cataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTaa                                                                                                            <  7:89925/148‑1 (MQ=255)
                                       ttaCATTTTTATTAGGGATTTATGGCTTTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGTGTATCAAGCCGGTGGGTAAAGCACCGGCTTGt                                                                                                                                    <  8:229384/122‑1 (MQ=255)
                                       ataCATTTTTATTAGGGATTTATTGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAAc                                                                                                           <  3:37490/148‑1 (MQ=255)
                                       ataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACt                                                                                                          >  8:316882/1‑149 (MQ=255)
                                       ataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCGTGTTACAAAGTAAGAATGGGAGTTTAACt                                                                                                          >  1:78984/1‑149 (MQ=255)
                                           aTTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGccc                                                                                                      >  2:169817/1‑149 (MQ=255)
                                           aTTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGccc                                                                                                      >  3:17455/1‑149 (MQ=255)
                                            tttttATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTg                                                                                                                                     <  6:303522/117‑1 (MQ=255)
                                            tttttATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTg                                                                                                                                     >  5:303522/1‑117 (MQ=255)
                                                  ttaGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACATGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGAc                                                                                               >  2:91294/1‑149 (MQ=255)
                                                  ttaGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATggg                                                                                                                   >  3:354576/1‑129 (MQ=255)
                                                  ttaGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATggg                                                                                                                   <  4:354576/129‑1 (MQ=255)
                                                  ttaGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGAc                                                                                               >  8:85332/1‑149 (MQ=255)
                                                     gggATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACttt                                                                                            >  6:169389/1‑149 (MQ=255)
                                                     gggATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACttt                                                                                            >  1:266217/1‑149 (MQ=255)
                                                      ggATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTg                                                                                           >  7:201275/1‑149 (MQ=255)
                                                      ggATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTg                                                                                           >  3:18351/1‑149 (MQ=255)
                                                        aTTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCa                                                                                         <  8:201127/149‑1 (MQ=255)
                                                             tGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATa                                                                                     <  7:85332/148‑1 (MQ=255)
                                                             tGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAg                                                                                    <  4:17455/149‑1 (MQ=255)
                                                                     tAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGTTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTAAAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGc                                                                                          <  4:206650/135‑1 (MQ=255)
                                                                     tAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGc                                                                                          >  3:206650/1‑135 (MQ=255)
                                                                      aaCTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCt                                                                           >  8:10173/1‑149 (MQ=255)
                                                                       aCTTAGTGTTGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGTATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCtt                                                                          <  2:78984/149‑1 (MQ=255)
                                                                       aCTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCtt                                                                          >  7:2409/1‑149 (MQ=255)
                                                                       aCTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCtt                                                                          >  2:298741/1‑149 (MQ=255)
                                                                       aCTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCtt                                                                          >  2:145941/1‑149 (MQ=255)
                                                                        cTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCttg                                                                         >  8:134288/1‑149 (MQ=255)
                                                                         tAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTATCTGCCCCAGCGACTTTGCAGATAGCTGACCGCttgt                                                                        >  8:77844/1‑149 (MQ=255)
                                                                         tAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCttgt                                                                        >  3:54611/1‑149 (MQ=255)
                                                                         tAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCttgt                                                                        >  7:342718/1‑149 (MQ=255)
                                                                           aGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCttgtt                                                                       <  7:316882/148‑1 (MQ=255)
                                                                           aGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCttgtt                                                                       <  1:395190/148‑1 (MQ=255)
                                                                           aGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCttgtt                                                                       <  2:369656/148‑1 (MQ=255)
                                                                           aGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCttgtt                                                                       <  4:18351/148‑1 (MQ=255)
                                                                           aGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCttgtt                                                                       <  5:169389/148‑1 (MQ=255)
                                                                            gtgtGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGa                                                                     <  7:84536/149‑1 (MQ=255)
                                                                                 gTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTg                                                                >  2:72854/1‑149 (MQ=255)
                                                                                 gTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTATAAGAGGGTAACAAGCCGGTGGGTAAAGCAACGGATTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTCACCGCTTGTTGAGTCTg                                                                >  3:350294/1‑149 (MQ=255)
                                                                                  ttAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGc                                                               >  7:122793/1‑149 (MQ=255)
                                                                                    aaTTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCgg                                                             >  8:142499/1‑149 (MQ=255)
                                                                                      tttGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGc                                                                                          <  3:369677/118‑1 (MQ=255)
                                                                                      tttGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGc                                                                                          >  4:369677/1‑118 (MQ=255)
                                                                                          aCTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTAtt                                                       <  5:348841/149‑1 (MQ=255)
                                                                                           cTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTAtt                                                       >  3:269747/1‑148 (MQ=255)
                                                                                           cTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTc                                                      <  7:134288/149‑1 (MQ=255)
                                                                                              aagtaagCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCa                                                     <  1:150111/147‑1 (MQ=255)
                                                                                                 taagCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAg                                                >  6:85780/1‑149 (MQ=255)
                                                                                                   agCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAg                                                >  3:367054/1‑147 (MQ=255)
                                                                                                   agCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAg                                                <  4:367054/147‑1 (MQ=255)
                                                                                                         attattAGTGTGATAGTTTAAGAGGTTAAAAAGCGGGTTGGTAAAGCAAAGGGTTGTTACAAAGTAATTATGTGAGTTTTACTGCCCCTGCGACTTTGCAGATAGCTGACCGCTTGTTGTGTCTGCGGTTTATTCAGATTGTCCTCACg                                        <  4:350294/149‑1 (MQ=255)
                                                                                                         attattAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCgg                                                             <  3:287022/128‑1 (MQ=255)
                                                                                                         attattAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCgg                                                             >  4:287022/1‑128 (MQ=255)
                                                                                                             ttaGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGaa                                     <  7:10173/148‑1 (MQ=255)
                                                                                                                gTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCCCCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAAc                                    >  6:89955/1‑146 (MQ=255)
                                                                                                                gTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAAc                                    >  8:72668/1‑146 (MQ=255)
                                                                                                                   ggATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGAt                               <  8:122793/148‑1 (MQ=255)
                                                                                                                   ggATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGAt                               <  5:386588/148‑1 (MQ=255)
                                                                                                                        gTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGc                          <  2:266217/148‑1 (MQ=255)
                                                                                                                         tttAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCg                        >  4:335623/1‑149 (MQ=255)
                                                                                                                          ttAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGc                       >  6:219266/1‑149 (MQ=255)
                                                                                                                             agagGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTaa                    >  2:256807/1‑149 (MQ=255)
                                                                                                                             agagGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTaa                    <  1:169817/149‑1 (MQ=255)
                                                                                                                                ttgTAACAAGCCGGTGCTTTACCCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGAt                               >  4:290100/3‑135 (MQ=38)
                                                                                                                                ttgTAACAAGCCGGTGCTTTACCCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGAt                               <  3:290100/133‑1 (MQ=38)
                                                                                                                                 ggTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCt                                                                                  >  3:100806/1‑83 (MQ=255)
                                                                                                                                 ggTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCt                                                                                  <  4:100806/83‑1 (MQ=255)
                                                                                                                                   tAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTATCTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCgg              <  7:77844/149‑1 (MQ=255)
                                                                                                                                   tAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCgg              <  5:219266/149‑1 (MQ=255)
                                                                                                                                     aCAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCggg             <  7:72668/148‑1 (MQ=255)
                                                                                                                                      cAAGCCGGGGGGTAACGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTACCTGCCCCAGCGACTTTGCCGAGAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGca           <  1:145941/149‑1 (MQ=255)
                                                                                                                                         gCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAg        >  5:20135/1‑149 (MQ=255)
                                                                                                                                               gggTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGt  >  4:258840/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGT  >  NC_000913/1567322‑1567613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: