Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,429,069 T→A 10.7% G7G (GGT→GGA stfR → Rac prophage; putative tail fiber protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,429,0690TA10.7% 243.5 / 19.0 94G7G (GGT→GGAstfRRac prophage; putative tail fiber protein
Reads supporting (aligned to +/- strand):  ref base T (35/48);  new base A (7/3);  total (42/52)
Fisher's exact test for biased strand distribution p-value = 1.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.41e-01

AGGCCCCGGCAGTGGCGACTGGCGCACTGACGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGT  >  NC_000913/1428921‑1429216
                                                                                                                                                    |                                                                                                                                                   
aGGCCCCGGCAGTGGCGACTGGCGCACTGACGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGgt                                                                                                                                                     <  6:119718/149‑1 (MQ=255)
aGGCCCCGGCAGTGGCGACTGGCGCACTGACGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGgt                                                                                                                                                     >  1:103822/1‑149 (MQ=255)
    cccGGCAGTGGCGACTGGCGCACTGACGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTAc                                                                                                                                                 >  8:196057/1‑149 (MQ=255)
    cccGGCAGTGGCGACTGGCGCACTGACGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCAGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTAc                                                                                                                                                 >  6:68009/1‑149 (MQ=255)
         cAGTGGCGACTGGCGCACTGACGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGaaa                                                                                                                                            <  3:226898/149‑1 (MQ=255)
          aGTGGCGACTGGAGCAATGTCGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGAAAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTCGGGTGAATATTGCAGTAAAGATTTCAGGTGTACTGa                                                                                                                                              >  6:78800/1‑146 (MQ=255)
            tGGCGACTGGCGCACTGACGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGAc                                                                                                                                         <  6:6208/149‑1 (MQ=255)
              gCGACTGGCGTACTGACGGATTCATCGTTGGGGTCGGTTATAAATTCTGATTAGCCAGGTAACACAGTGTTATGACACCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGAAGTAAAGATTTCAGGAGTCCTGAAAGACgg                                                                                                                                       >  3:172296/1‑149 (MQ=255)
              gCGACTGGCGCACTGACGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACgg                                                                                                                                       <  2:103822/149‑1 (MQ=255)
              gCGACTGGCGCACTGACGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACgg                                                                                                                                       <  1:169533/149‑1 (MQ=255)
              gCGACTGGCGCACTGACGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACgg                                                                                                                                       <  7:377049/149‑1 (MQ=255)
              gCGACTGGCGCACTGACGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACgg                                                                                                                                       <  5:68009/149‑1 (MQ=255)
                   tGGCGCACTGACGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAg                                                                                                                                  >  1:141633/1‑149 (MQ=255)
                       gcACTGACGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGaa                                                                                                                               >  3:50403/1‑148 (MQ=255)
                        taCTGACGGATTCATCGTTGGGGTCGGTTATAAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGaaaa                                                                                                                             >  2:259696/2‑149 (MQ=255)
                        cACTGACGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGaaaa                                                                                                                             <  2:141633/149‑1 (MQ=255)
                         aCTTACGGTTTCAGCGTGGGTGGCGGTTATAAGTTCTGATTAGCCTGGTAACACAGGGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAAc                                                                                                                            <  6:163513/149‑1 (MQ=255)
                           tGACGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACCGGAAAACCg                                                                                                                          >  4:360390/1‑149 (MQ=255)
                           tGACGGATTCATCGTTGGGGTCGGTTATAAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCg                                                                                                                          >  4:242169/1‑149 (MQ=255)
                             aCGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCTGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCg                                                                                                                          <  7:273271/147‑1 (MQ=255)
                             aCGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCgg                                                                                                                         <  2:85675/148‑1 (MQ=255)
                             aCGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGt                                                                                                                        <  7:122862/149‑1 (MQ=255)
                             aCGGTTTCATCGTGGGTGCCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCgg                                                                                                                         <  5:81581/148‑1 (MQ=255)
                             aCGGATTCATCGTTTGGGTCGGTTATAAATTCTGATTAGGCAGGTAACAGATTTTTATGACAGCCCGCCGGAACCGGTGGGATTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCg                                                                                                                          <  4:172296/147‑1 (MQ=255)
                              cGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTa                                                                                                                       <  7:196057/149‑1 (MQ=255)
                                   tCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGa                                                                                                                   <  1:313342/148‑1 (MQ=255)
                                   tCATCGGGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTCACCCAGTGTTATGACAGCCCGCCGGTGCAGGCGGGCGTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGGGTACTGAAAGACGGCACAGGAAACCCGGTACAGa                                                                                                                   <  1:307926/148‑1 (MQ=255)
                                    cATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAAc                                                                                                                 >  3:234807/1‑149 (MQ=255)
                                     aTCGTTGGAGTCGGTTAAAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGTGGTGAATAAGGCAGTAAAGATTTCAGGTGTACTCAAAGACGGCACAGGAACACCGGTACAGAACt                                                                                                                >  8:242040/1‑149 (MQ=255)
                                     aTCGTGGGTGTCGGTTATAAGTTCTGATTAGACAGGTAACACAGTGTTATGACATCCCGACGGTTAAGGCTTGCTTTTTTGTGGGGTGAATATTGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACt                                                                                                                <  5:78800/149‑1 (MQ=255)
                                      tCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTg                                                                                                               >  4:378080/1‑149 (MQ=255)
                                      tCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTAAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGCCGGCACAGGAAAACCGGTACAGAACTg                                                                                                               >  6:42562/1‑149 (MQ=255)
                                        gtgtgtgtCGGTTTTAAGTTCTGTTTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTTCTGAAAGACGGCACAGGATAACCGGTACAGAACTGca                                                                                                             <  8:298640/149‑1 (MQ=255)
                                        gTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGca                                                                                                             <  6:152386/149‑1 (MQ=255)
                                        gTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGc                                                                                                              <  3:199314/148‑1 (MQ=255)
                                         tGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGca                                                                                                             >  8:272359/1‑148 (MQ=255)
                                               tCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATc                                                                                                        >  2:196759/1‑147 (MQ=255)
                                               tCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATc                                                                                                        >  7:413364/1‑147 (MQ=255)
                                               tCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATc                                                                                                        >  3:155731/1‑147 (MQ=255)
                                                      taAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGaaa                                                                                               <  6:136533/149‑1 (MQ=255)
                                                       aaattCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAg                                                                                              >  6:42843/4‑149 (MQ=255)
                                                       aaGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGa                                                                                                 <  2:47272/146‑1 (MQ=255)
                                                       aaGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGa                                                                                                 >  1:47272/1‑146 (MQ=255)
                                                       aaGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCAGGTTCAGGCGGGCTTTTTTGTGGGGTGAAAATGGCAGTAAAGATTTAAGGTGTACTGAAAGACGGAACAGCAAAACAGGTACAGAACTGCACAATCAAGCTCaaac                                                                                              >  2:277857/1‑148 (MQ=255)
                                                        aGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGTGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGc                                                                                             <  5:346267/149‑1 (MQ=255)
                                                        aGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGc                                                                                             <  7:272359/149‑1 (MQ=255)
                                                          ttCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCa                                                                                            >  2:357244/1‑148 (MQ=255)
                                                              gATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACAGGTACAGAACTGCACAATCCAGCAGAAAGCAAAACg                                                                                       >  1:36532/1‑149 (MQ=255)
                                                               aTTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACg                                                                                       >  6:132791/1‑148 (MQ=255)
                                                               aTTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACg                                                                                       <  2:65613/148‑1 (MQ=255)
                                                                   gCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTaa                                                                                    >  1:176766/1‑147 (MQ=255)
                                                                      aGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGca                                                                               >  7:342172/1‑149 (MQ=255)
                                                                        gTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGcac                                                                              >  3:17006/1‑148 (MQ=255)
                                                                          aaCACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGgt                                                                                                                                                     >  1:227918/1‑75 (MQ=255)
                                                                          aaCACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGgt                                                                                                                                                     <  2:227918/75‑1 (MQ=255)
                                                                            cacaGAGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGAACCACgg                                                                         >  3:21314/1‑149 (MQ=255)
                                                                                 tgtTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACggtggtg                                                                    <  1:259696/149‑1 (MQ=255)
                                                                                   ttttGACAGCCCGGCGGTTGAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACggtggtggt                                                                  <  4:21314/146‑1 (MQ=255)
                                                                                   ttATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACggtggtgg                                                                   <  4:155731/148‑1 (MQ=255)
                                                                                   ttATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACggtggtgg                                                                   <  6:131867/148‑1 (MQ=255)
                                                                                   ttATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACggtggtgg                                                                   <  1:277857/148‑1 (MQ=255)
                                                                                      tGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGaa                                                               >  4:108215/1‑149 (MQ=255)
                                                                                       gACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAac                                                              >  5:289743/1‑149 (MQ=255)
                                                                                        aCAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAac                                                              <  8:413364/148‑1 (MQ=255)
                                                                                         cAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAacac                                                            >  8:103525/1‑149 (MQ=255)
                                                                                           gCCCGCCGGAACCGGTGGGCTTTTTTGTGTGGTGAATATGGCAGTAAAGATTTCAGGAGTCATGAAAGACGGCACAGTAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTTGTGAACACgg                                                          >  6:57356/1‑148 (MQ=255)
                                                                                                   gTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATc                                                                                                        <  8:198470/95‑1 (MQ=255)
                                                                                                   gTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATc                                                                                                        >  7:198470/1‑95 (MQ=255)
                                                                                                               ttttttGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGAt                                      <  4:64991/149‑1 (MQ=255)
                                                                                                                 ttttGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATg                                     >  7:324970/1‑148 (MQ=255)
                                                                                                                     gtgGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGcc                                >  5:139984/1‑149 (MQ=255)
                                                                                                                      tgGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGcc                                <  3:322371/148‑1 (MQ=255)
                                                                                                                      tgGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCg                               <  2:176766/149‑1 (MQ=255)
                                                                                                                        gggTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTAAAGAACTGAACAATCCAGCTGAAAGCAAAAAGTAACAGCACCACGGTGGTGGTGACCACGCTGCCCTCAGAAAATCCGGATGAAGCCggg                             >  5:207159/1‑149 (MQ=255)
                                                                                                                         ggTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGc                            <  5:114439/149‑1 (MQ=255)
                                                                                                                           tGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGt                          <  5:132791/149‑1 (MQ=255)
                                                                                                                             aaTATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGtt                         <  3:378080/148‑1 (MQ=255)
                                                                                                                              atatGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTAc                       <  3:242169/149‑1 (MQ=255)
                                                                                                                                 tGGCAGTAAAGATTTCAGGTGTTCTGAAAGACGTCACAGGAAAACCGGTACTGAACTGGACAATCCAGCTGAAACGAAAACGTAACAGCACCACGGTGGTTGTGAACACGCTGTCCTCAGAAATTCCGGATGAAGCCGGGCGTTACAg                     <  3:287775/148‑1 (MQ=255)
                                                                                                                                   gCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCa                   <  1:357244/148‑1 (MQ=255)
                                                                                                                                    cAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATg                 <  4:50403/149‑1 (MQ=255)
                                                                                                                                     aGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAAc                                                                                   >  6:253893/1‑82 (MQ=255)
                                                                                                                                     aGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAAc                                                                                   <  5:253893/82‑1 (MQ=255)
                                                                                                                                     aGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCAAAATCAAGCTGAAAGCAAAACGTAACAGCACCAAGGTGGTGGTGAAAAAGCTGGCCTCATAAAATCCTTATGAAGACGGTCGTTACAGCATg                 >  1:403860/1‑148 (MQ=255)
                                                                                                                                      gTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGa               >  4:131771/1‑149 (MQ=255)
                                                                                                                                      gTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGa               >  4:211108/1‑149 (MQ=255)
                                                                                                                                       tAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGAc              >  2:77119/1‑149 (MQ=255)
                                                                                                                                         aaGTTTTCAGGTGTACTGAAAGACGTCACAGGAAAACCGGTACATAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGATCACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGt            <  6:207159/149‑1 (MQ=255)
                                                                                                                                          aGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTTCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTTGCCTCAGAAAATCCGGATTAAGCCGGGCGTTACAGCATGGACGtt           <  7:242040/149‑1 (MQ=255)
                                                                                                                                          aGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCgg                              <  1:309154/130‑1 (MQ=255)
                                                                                                                                          aGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCgg                              >  2:309154/1‑130 (MQ=255)
                                                                                                                                          aGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGtt           <  6:139984/149‑1 (MQ=255)
                                                                                                                                          aGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGtt           <  7:36967/149‑1 (MQ=255)
                                                                                                                                          aGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGtt           <  1:196759/149‑1 (MQ=255)
                                                                                                                                          aGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGtt           <  3:211108/149‑1 (MQ=255)
                                                                                                                                               tCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCTTTACAGCATGGACGTTGAGt       <  5:42562/148‑1 (MQ=255)
                                                                                                                                               tCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACAGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTa      >  7:245071/1‑149 (MQ=255)
                                                                                                                                                 aGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACg    <  5:328771/149‑1 (MQ=255)
                                                                                                                                                    tgtACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGt  >  3:311530/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AGGCCCCGGCAGTGGCGACTGGCGCACTGACGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGT  >  NC_000913/1428921‑1429216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: