Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 756,423 C→T 24.3% Q173* (CAG→TAG)  sdhA → succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913756,4230CT24.3% 142.6 / 46.3 78Q173* (CAG→TAG) sdhAsuccinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base C (25/34);  new base T (9/10);  total (34/44)
Fisher's exact test for biased strand distribution p-value = 7.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.45e-01

AAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACG  >  NC_000913/756276‑756570
                                                                                                                                                     |                                                                                                                                                   
gcggtcagtcgaaaaacttcggcggcACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCa                                                                                                                                                    <  2:392340‑M2/123‑1 (MQ=255)
 aaaaCTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAg                                                                                                                                                   >  6:146222/1‑149 (MQ=255)
   aaCTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGa                                                                                                                                                 <  7:36199/149‑1 (MQ=255)
     cTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTTTCTCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAACCCAGGa                                                                                                                                                 >  4:14610/1‑149 (MQ=255)
      agtcgaaaaacttcggcggcACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCAAACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATgg                                                                                                                                              >  4:330623‑M2/21‑149 (MQ=255)
          gaaaaacttcggcggcACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGC‑‑TTTATCAGCAGAACCTGAAAAACCACCCCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGcgcg                                                                                                                                          >  1:120138‑M2/17‑149 (MQ=255)
            aaaacttcggcggcACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCggt                                                                                                                                        <  2:120138‑M2/135‑1 (MQ=255)
             gcgAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCggtg                                                                                                                                       <  4:357269/149‑1 (MQ=255)
                  cAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCGCACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTggg                                                                                                                                  <  1:11504/149‑1 (MQ=255)
                  cAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACTAGGATGGCGCGGtggtgg                                                                                                                                   >  5:307034/1‑148 (MQ=255)
                  cAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACTAGGATGGCGCGGtggtgg                                                                                                                                   <  6:307034/148‑1 (MQ=255)
                  cAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCggtggt                                                                                                                                     >  3:81455/1‑146 (MQ=255)
                  cAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCggtggt                                                                                                                                     <  4:81455/146‑1 (MQ=255)
                   aGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTTTCTCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGGGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGtggtgg                                                                                                                                   <  8:354022/149‑1 (MQ=255)
                    ggcggcACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAGAACCAGGATGGCGCGGTGGTGGGtt                                                                                                                                >  8:268866‑M2/7‑149 (MQ=255)
                      cggcACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTg                                                                                                                               >  2:278602‑M2/5‑148 (MQ=255)
                       ggcACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACTAGGATGGCGCGGTGGTGGGTTGTa                                                                                                                             >  5:152744‑M2/4‑149 (MQ=255)
                       ggcACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTa                                                                                                                             <  1:56747‑M2/146‑1 (MQ=255)
                       ggcACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTa                                                                                                                             <  5:246268‑M2/146‑1 (MQ=255)
                          acgcacTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCg                                                                                                                          >  3:309276/1‑149 (MQ=255)
                                tGCGGCAGCAGCTGACCGTACCGGTCACGCAATGTTGCAAACTC‑‑TTTATCAGCAGAACCTGAAAAACCACACCAACATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATCGCGCGGGGGTGGGTTGTAACGAACtgt                                                                                                                    >  6:278951/1‑149 (MQ=255)
                                tGCGGCAGCAGCTGACCGTACCGGTAACGAACTGTTTCTCACTC‑‑TTTATCAGCAGAACCTTAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCTTTTGTGGGTTGTACCGCACtgt                                                                                                                    <  5:278951/149‑1 (MQ=255)
                                  cGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACtgt                                                                                                                    <  6:54443/147‑1 (MQ=255)
                                     cagcagCTGACCGTACCGGTCACGCACTGTTGCACACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACTAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATc                                                                                                               <  6:257142/149‑1 (MQ=255)
                                     cagcagCTGACCGTACCGGTCACGCACTGTTGCACACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACTAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATc                                                                                                               <  6:152744/149‑1 (MQ=255)
                                              aCCGTACCGGTCACGCACTGTTGCACACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCg                                                                                                        <  5:301694/147‑1 (MQ=255)
                                                 gTACCGGTCACGCACTGTTGCACACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGaa                                                                                                   <  4:309276/149‑1 (MQ=255)
                                                  tACCGGTCACGCACTGTTGCACACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACTAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGt                                                                                                      <  3:184717/145‑1 (MQ=255)
                                                  tACCGGTCACGCACTGTTGCACACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACTAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGt                                                                                                      >  4:184717/1‑145 (MQ=255)
                                                    ccGGTCACGCACTGTTGCACACGC‑‑TTTATCAGCAGACCCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTg                                                                                                >  2:226493/1‑149 (MQ=255)
                                                     cGGTCACGCACTGTTGCACACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTgg                                                                                               >  5:246050/1‑149 (MQ=255)
                                                      ggTCACGCACTGTTGCACACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGt                                                                                              <  8:314224/149‑1 (MQ=255)
                                                       gTCACGCACTGTTGCACACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACTAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGtt                                                                                             <  8:297832/149‑1 (MQ=255)
                                                       gTCACGCACTGTTGCACACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCAc                                                                                                                       >  7:251455/1‑123 (MQ=255)
                                                       gTCACGCACTGTTGCACACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCAc                                                                                                                       <  8:251455/123‑1 (MQ=255)
                                                       gTCACGCACTGTTGCACACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGtt                                                                                             >  7:260632/1‑149 (MQ=255)
                                                       gTCACGCACTGTTGCACACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGGGAAAAACTAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAAc                                                                                                          <  4:285606/136‑1 (MQ=255)
                                                       gTCACGCACTGTTGCACACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGCGAAAAACTAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAAc                                                                                                          >  3:285606/1‑136 (MQ=255)
                                                        tCAGGCACTGTTGCACACGC‑‑TTTATCAGGAGAACCTGAAAAACCACCCCACCATTTTCTCCGAGTGGTATTCGCTGGATCTGGTGAAAAACCAGGTTGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGt                                                                                              <  1:251973/147‑1 (MQ=255)
                                                           cGCACTGTTGCACACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACTAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTAtt                                                                                         >  4:258046/1‑149 (MQ=255)
                                                              aCTGTTGCACACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCa                                                                                      <  6:174576/149‑1 (MQ=255)
                                                                tGTTGCACACGC‑‑TTTATCGGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGt                                                                                                      <  8:226424/131‑1 (MQ=255)
                                                                tGTTGCACACGC‑‑TTTATCGGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGt                                                                                                      >  7:226424/1‑131 (MQ=255)
                                                                     cacaCGC‑‑TTTATCAGCAGAACCTGAAAATCCACACCTCCTTTTTGTCCGAGTGGTATGCGCTGGATCTGGTGAATAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTg                                                                                                <  2:283910/132‑1 (MQ=255)
                                                                     cacaCGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAg                                                                                                  >  1:400164/1‑130 (MQ=255)
                                                                     cacaCGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAg                                                                                                  <  2:400164/130‑1 (MQ=255)
                                                                      acacGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCcgc                                                                              >  6:394376/1‑149 (MQ=255)
                                                                        acGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACTAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCgcgc                                                                            >  2:116623/1‑149 (MQ=255)
                                                                          gC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGcc                                                                                 <  4:272021/142‑1 (MQ=255)
                                                                          gC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGcc                                                                                 >  3:272021/1‑142 (MQ=255)
                                                                          gC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTa                                                                          >  7:35094/1‑149 (MQ=255)
                                                                              tttATCAGCAGAACCTGAAAAACCACACCACCATTTTCTGCGAGTGGTATGCGCTAGATCTGGTGAAAAACCAGGATGGCTCGGTGGTGGGTTGTACCGAAGTGTGCATGGAAACCGTTGAAGTGGTTTTTTTAAATGCCCGCGCTAcc                                                                        >  1:359707/1‑149 (MQ=255)
                                                                                  tCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTg                                                                     <  5:394376/148‑1 (MQ=255)
                                                                                     gcagAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACTAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCg                                                                                                        <  2:365506/110‑1 (MQ=255)
                                                                                     gcagAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACTAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCg                                                                                                        >  1:365506/1‑110 (MQ=255)
                                                                                      cagAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGt                                                                                                      <  2:216813/111‑1 (MQ=255)
                                                                                      cagAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGt                                                                                                      >  1:216813/1‑111 (MQ=255)
                                                                                            cTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGTGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTgg                                                          <  3:330623/149‑1 (MQ=255)
                                                                                            cTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTgg                                                          >  2:154869/1‑149 (MQ=255)
                                                                                            cTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTgg                                                          >  5:223149/1‑149 (MQ=255)
                                                                                             tGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGc                                                         >  1:362366/1‑149 (MQ=255)
                                                                                               aaaaaCCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGc                                                         <  8:330968/147‑1 (MQ=255)
                                                                                                 aaaCCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAg                                                     <  2:362366/149‑1 (MQ=255)
                                                                                                       caccacCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGc                                               <  8:35094/149‑1 (MQ=255)
                                                                                                           accaTTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACTAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTAt                                           <  2:38087/149‑1 (MQ=255)
                                                                                                            ccaTTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACTAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTAtt                                          <  3:258046/149‑1 (MQ=255)
                                                                                                             caTTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACTAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTAtt                                          >  7:301256/1‑148 (MQ=255)
                                                                                                                 ttCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATc                                      <  1:154869/148‑1 (MQ=255)
                                                                                                                  tctcCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCa                                     <  6:223149/148‑1 (MQ=255)
                                                                                                                      cGAGTGGTATGCGCTGGATCTGGTGAAAAACTAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGc                                                                >  1:235271/1‑117 (MQ=255)
                                                                                                                      cGAGTGGTATGCGCTGGATCTGGTGAAAAACTAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGc                                                                <  2:235271/117‑1 (MQ=255)
                                                                                                                      cGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGTCTGGCGGAGCAGGGCGTATTTATCAGTcc                                 <  1:226493/148‑1 (MQ=255)
                                                                                                                                      gATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGa                                                                                                    >  6:62163/1‑65 (MQ=255)
                                                                                                                                      gATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGa                                                                                                    <  5:62163/65‑1 (MQ=255)
                                                                                                                                            gTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAac            <  5:146222/147‑1 (MQ=255)
                                                                                                                                            gTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCACGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGAGTATTTATCAGTCCACCACCAACGCCCACATTAacac          >  6:361675/1‑149 (MQ=255)
                                                                                                                                             ggAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACAcc         <  1:229865/148‑1 (MQ=255)
                                                                                                                                                   aCCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGAc   <  8:260632/149‑1 (MQ=255)
                                                                                                                                                    ccAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACg  <  7:268866/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
AAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGC‑‑TTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCACTGTGCATCGAAACCGGTGAAGTGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACG  >  NC_000913/756276‑756570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: