Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 517,383 A→T 6.9% intergenic (+21/+42) fetB → / ← ybbN iron export ABC transporter permease; peroxide resistance protein/DnaK co‑chaperone, thioredoxin‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913517,3830AT 6.9% 273.2 / 8.6 87intergenic (+21/+42)fetB/ybbNiron export ABC transporter permease; peroxide resistance protein/DnaK co‑chaperone, thioredoxin‑like protein
Reads supporting (aligned to +/- strand):  ref base A (41/40);  new base T (3/3);  total (44/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

ATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGTTTTACGCGTCTGACCGTCTG  >  NC_000913/517237‑517531
                                                                                                                                                  |                                                                                                                                                    
aTTAGGGGGACCTTTATGCTGCTCGCACCCGCCAGCTTGGCGCCCATTATTGCCTGCTATTTACCCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAgcatc                                                                                                                                                    <  5:327481/149‑1 (MQ=255)
 ttATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAgcatcg                                                                                                                                                   <  5:92080/149‑1 (MQ=255)
    tGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAgcatcgcat                                                                                                                                                >  3:126799/1‑149 (MQ=255)
      gTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACTCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAgcatcgcatc                                                                                                                                               <  5:241741/148‑1 (MQ=255)
      gTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATcc                                                                                                                                              >  5:84712/1‑149 (MQ=255)
      gTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATcc                                                                                                                                              >  7:38835/1‑149 (MQ=255)
       tGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGGGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCg                                                                                                                                             <  8:154588/149‑1 (MQ=255)
          ccTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCg                                                                                                                                          <  5:164263/149‑1 (MQ=255)
                tgctgcTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTgg                                                                                                                                    <  6:239821/149‑1 (MQ=255)
                   tgctCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGtt                                                                                                                                  >  7:223902/1‑148 (MQ=255)
                   tgctCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGtt                                                                                                                                  >  3:151973/1‑148 (MQ=255)
                   tgctCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGATGCTGCGCATCCGGcatc                                                                                                                                        <  2:157264/142‑4 (MQ=255)
                   tgctCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGATGCTGCGCATCCGGcatc                                                                                                                                        >  1:157264/1‑139 (MQ=255)
                      tctcAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCAtg                                                                                                                              >  5:260011/1‑149 (MQ=255)
                       ctcAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAgcatc                                                                                                                                                    <  4:155370/126‑1 (MQ=255)
                       ctcAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAgcatc                                                                                                                                                    >  3:155370/1‑126 (MQ=255)
                        tcAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCAtg                                                                                                                              <  8:38835/147‑1 (MQ=255)
                          aaCCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCAtgt                                                                                                                             <  8:321430/146‑1 (MQ=255)
                          aaCCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCAt                                                                                                                               <  1:376668/144‑1 (MQ=255)
                          aaCCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCAt                                                                                                                               >  2:376668/1‑144 (MQ=255)
                          aaCCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGcc                                                                                                                          <  1:245313/149‑1 (MQ=255)
                            ccGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGa                                                                                                                        >  8:406975/1‑149 (MQ=255)
                             cGCCTGCTTGTCGAACATTATTGCCTGCTCTTTAACCTCTCGTACGTGTTATAATTCGCCCCACCAGTTGGTGGTGACGCAATTGAAGAAGAACTGATGCGATGCCGGATGCGCATCATCGCATCCGGCGTTGTGGTTCATGTGCCGAt                                                                                                                       <  2:168668/149‑1 (MQ=255)
                             cGCCAGCTTGTCGCCCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCCCCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATTTGCCGAt                                                                                                                       >  3:337129/1‑149 (MQ=255)
                             cGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGAt                                                                                                                       <  8:296258/149‑1 (MQ=255)
                                cAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCaa                                                                                                                    >  4:43945/1‑149 (MQ=255)
                                       tCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAata                                                                                                             >  2:414932/1‑149 (MQ=255)
                                        cgtccATTATTTCCTTCTATTTAACCTATCGTTAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAatat                                                                                                            <  4:136371/146‑1 (MQ=255)
                                         gACCATAATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACACAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAatat                                                                                                            <  6:377668/148‑1 (MQ=255)
                                           ccATTATTGCCTGCTATTTACCCTCTCGGCAGTTGTATACTTCGCGACACCAGGTGGTGGGGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCaa                                                                                                         <  4:337129/149‑1 (MQ=255)
                                           ccATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCaa                                                                                                         <  6:84712/149‑1 (MQ=255)
                                            cATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCaa                                                                                                         >  4:312708/1‑148 (MQ=255)
                                             attattGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATa                                                                                                       <  4:151973/149‑1 (MQ=255)
                                                   gCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATacaaca                                                                                                  <  7:406975/148‑1 (MQ=255)
                                                    ccTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAAt                                                                                                >  3:147835/1‑149 (MQ=255)
                                                       gCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCaa                                                                                                         >  8:30815/1‑137 (MQ=255)
                                                       gCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCaa                                                                                                         <  7:30815/137‑1 (MQ=255)
                                                        cTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCa                                                                                             >  4:301937/1‑148 (MQ=255)
                                                          aTTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATa                                                                                           >  1:413668/1‑148 (MQ=255)
                                                               aCCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTg                                                                                     <  1:414932/149‑1 (MQ=255)
                                                               aCCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTg                                                                                     >  6:275792/1‑149 (MQ=255)
                                                                ccTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTgg                                                                                    >  6:313055/1‑149 (MQ=255)
                                                                 cTATCGTAAGTTTTATAATTAGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATTCCGGATGCGCAGCATCGCATCCGGCGTGGTGGTTCATGTGCCGAGCAACGGAATATCAATACAACAATGCATACCGCTggc                                                                                   >  6:289074/1‑149 (MQ=255)
                                                                   aTCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCa                                                                                                          <  5:358664/124‑1 (MQ=255)
                                                                   aTCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCa                                                                                                          >  6:358664/1‑124 (MQ=255)
                                                                    tCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTggcgg                                                                                 <  3:301937/148‑1 (MQ=255)
                                                                    tCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTggcg                                                                                  <  7:122913/147‑1 (MQ=255)
                                                                      gTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCTTCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGa                                                                              >  5:96289/1‑149 (MQ=255)
                                                                      gTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGa                                                                              >  3:69343/1‑149 (MQ=255)
                                                                      gTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGa                                                                              <  4:170448/149‑1 (MQ=255)
                                                                      gTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGa                                                                              <  2:321998/149‑1 (MQ=255)
                                                                      gTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGa                                                                              <  5:127768/149‑1 (MQ=255)
                                                                       tAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGa                                                                              >  1:39319/1‑148 (MQ=255)
                                                                       tAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGa                                                                              >  4:57663/1‑148 (MQ=255)
                                                                       tAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGa                                                                              >  1:391561/1‑148 (MQ=255)
                                                                         aGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCTTCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATa                                                                            <  6:96289/148‑1 (MQ=255)
                                                                            tttATAATTCGCGCCACCAGTTGGTGGAGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGAGGATCATGTGCCGATCAACGGAATATCAATAATAAAATGCATACAGCTGGCCGCGAAACTTc                                                                        >  4:316510/1‑149 (MQ=255)
                                                                             ttATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAat                                                                                                              <  2:104636/110‑1 (MQ=255)
                                                                             ttATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAat                                                                                                              >  1:104636/1‑110 (MQ=255)
                                                                             ttATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTc                                                                        <  1:309670/148‑1 (MQ=255)
                                                                                 aaTTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGc                                                                   >  4:116451/1‑149 (MQ=255)
                                                                                      gcgcCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGt                                                               <  2:391561/148‑1 (MQ=255)
                                                                                      gcgcCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTg                                                              >  3:221781/1‑149 (MQ=255)
                                                                                      gcgcCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGGGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGt                                                               <  1:268035/148‑1 (MQ=255)
                                                                                       cgcCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGc                                                             <  5:313055/149‑1 (MQ=255)
                                                                                          caccaGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATc                                                          <  3:116451/149‑1 (MQ=255)
                                                                                               gTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGGTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCg                                                     <  3:224693/149‑1 (MQ=255)
                                                                                                ttGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGt                                                    <  3:57663/149‑1 (MQ=255)
                                                                                                    tggtgACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTAccc                                                <  8:223902/149‑1 (MQ=255)
                                                                                                      gtgACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCcag                                              >  5:371187/1‑149 (MQ=255)
                                                                                                           gCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCcagcgcag                                         >  5:81014/1‑149 (MQ=255)
                                                                                                             aaTTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCAc                                                        >  5:282325/1‑132 (MQ=255)
                                                                                                             aaTTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCAc                                                        <  6:282325/132‑1 (MQ=255)
                                                                                                             aaTTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCcagcgcagc                                        >  4:257345/1‑148 (MQ=255)
                                                                                                              aTTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGa                                      <  4:126799/149‑1 (MQ=255)
                                                                                                               ttGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAg                                     >  5:154747/1‑149 (MQ=255)
                                                                                                                tGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAg                                     >  4:304832/1‑148 (MQ=255)
                                                                                                                  aagaagaaATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGAt                                  <  5:275792/149‑1 (MQ=255)
                                                                                                                   agaagaaATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGAtc                                 >  8:143194/1‑149 (MQ=255)
                                                                                                                   agaagaaATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGAtc                                 >  4:366043/1‑149 (MQ=255)
                                                                                                                     aagaaATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGAtctc                               >  3:140536/1‑149 (MQ=255)
                                                                                                                     aagaaATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGAtctc                               <  3:366043/149‑1 (MQ=255)
                                                                                                                            gatgcgatgcCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAAc                        >  1:214975/1‑149 (MQ=255)
                                                                                                                                cgatgcCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGttt                    <  5:31670/149‑1 (MQ=255)
                                                                                                                                cgatgcCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGtt                     <  3:43945/148‑1 (MQ=255)
                                                                                                                                cgatgcCGGATGCGCAGCATCGCATCCGGCGTTGTGGTACATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCTGTGCATCa                                                         >  5:385724/1‑112 (MQ=255)
                                                                                                                                 gatgcCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGCCGCCAGTGCATCACCCGTCCCCAGCGCAGCGAGGATCTCCTGGAACGttt                    >  2:342410/1‑148 (MQ=255)
                                                                                                                                  atgcCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGTTTTa                  >  7:165614/1‑149 (MQ=255)
                                                                                                                                    ggcGGATGCGATGCTGCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATCCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCcagcgcagcg                                       <  2:299541/124‑1 (MQ=255)
                                                                                                                                     ccGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGTTTTAcgc               <  2:413668/149‑1 (MQ=255)
                                                                                                                                     ccGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAAGACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGTTTTAcgc               >  5:202540/1‑149 (MQ=255)
                                                                                                                                      cGGATGCGATGCTGCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGTTTTAcgcg              >  4:7564/1‑149 (MQ=255)
                                                                                                                                            cgcAGCATCGCATCCTGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATAATACAATGCATACAGCTGGCGGGGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGTTTTACGCGTCTGa         <  3:316510/148‑1 (MQ=255)
                                                                                                                                             gcagcaTCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGTTTTACGCGTCTGAcc       >  5:390587/1‑149 (MQ=255)
                                                                                                                                                gcatcgcatcCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGTTTTACGCGTCTGACCGTc    <  4:147835/149‑1 (MQ=255)
                                                                                                                                                  atcgcagcCGGCGATGTGGTTCATGTGCCGATCAACGGAATCTCAATCCAACAATCCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCCCAGCGAGGATCTCCTGGAACGGTTTACGCGTCTGACCGTCTg  <  6:81014/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
ATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTAAGTTTTATAATTCGCGCCACCAGTTGGTGGTGACGCAATTGAAGAAGAAATGATGCGATGCCGGATGCGCAGCATCGCATCCGGCGTTGTGGTTCATGTGCCGATCAACGGAATATCAATACAACAATGCATACAGCTGGCGGCGATACTTCGACGCCAGTGCATCACCCGTACCCAGCGCAGCGAGGATCTCCTGGAACGTTTTACGCGTCTGACCGTCTG  >  NC_000913/517237‑517531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: