Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,599,416 A→G 5.2% L9P (CTT→CCT)  opgB ← OPG periplasmic biosynthetic phosphoglycerol transferases I (membrane‑bound) and II (soluble)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,599,4160AG 5.2% 376.0 / 7.0 115L9P (CTT→CCT) opgBOPG periplasmic biosynthetic phosphoglycerol transferases I (membrane‑bound) and II (soluble)
Reads supporting (aligned to +/- strand):  ref base A (55/54);  new base G (3/3);  total (58/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01

Only 100 of 120 total aligned reads displayed.

TCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGTAATGGCGTCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAAT  >  NC_000913/4599269‑4599563
                                                                                                                                                   |                                                                                                                                                   
tcgtTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAg                                                                                                                                                    >  8:67115/1‑149 (MQ=255)
  gtTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAggg                                                                                                                                                  <  5:279235/149‑1 (MQ=255)
   ttAATACCATCGCCAGTAAAATAGTCGCTTGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGc                                                                                                                                                 <  6:327635/149‑1 (MQ=255)
     aaTACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCg                                                                                                                                                >  2:293358/1‑148 (MQ=255)
        acgaTCGCGTGTAAAATAGTCGCTGGCAAACAGGGTGATTTTTAAAAGGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGTTGTTTCGTCCCCCTTTCCATGCGTAAATCAGCACAGAGGCGAGAATAAGGGCGaaa                                                                                                                                             <  4:8618/145‑1 (MQ=255)
          cATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGaaa                                                                                                                                             >  1:215069/1‑146 (MQ=255)
           aTCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAagaga                                                                                                                                         <  8:133159/149‑1 (MQ=255)
            tCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGATAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAaga                                                                                                                                           >  7:158002/1‑146 (MQ=255)
            tCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCCCCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAagagag                                                                                                                                        >  8:218050/1‑149 (MQ=255)
            tCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAagagag                                                                                                                                        <  2:373237/149‑1 (MQ=255)
                cAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCAAAGCGTAAATCAGCAAAGAGGCGAGAAAAAGGGCGAAAGAGagaag                                                                                                                                     >  5:53241/1‑148 (MQ=255)
                 aGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGtt                                                                                                                                   <  7:221160/149‑1 (MQ=255)
                     aaaTAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTg                                                                                                                                <  2:144786/148‑1 (MQ=255)
                           tCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACgg                                                                                                                         >  1:375083/1‑149 (MQ=255)
                                 gCAAACAGGGTGATATTTAAAACGAAAAATAGCCCCAGCACCGTTAACGTGGAGGCAAACCACAAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATc                                                                                                                   <  2:306825/149‑1 (MQ=255)
                                   aaaCAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCt                                                                                                                 >  7:125323/1‑149 (MQ=255)
                                   aaaCAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCt                                                                                                                 <  5:325330/149‑1 (MQ=255)
                                       aGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCAc                                                                                                              >  6:45860/1‑148 (MQ=255)
                                           tGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTGAATCAGCACAGAGGCGAGAAAAGGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAAc                                                                                                         >  5:354466/1‑149 (MQ=255)
                                           tGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTGAATCAGCACAGAGGCGAGAAAAGGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAAc                                                                                                         >  7:186769/1‑149 (MQ=255)
                                           tGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAAc                                                                                                         >  4:246844/1‑149 (MQ=255)
                                             atatTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCg                                                                                                       <  2:272282/149‑1 (MQ=255)
                                               atTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAAc                                                                                                                           <  7:135999/127‑1 (MQ=255)
                                               atTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAAc                                                                                                                           >  8:135999/1‑127 (MQ=255)
                                               atTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCggg                                                                                                     >  8:128401/1‑149 (MQ=255)
                                                tttAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGTCGAAAGAGAGTAGTTCTGACAACggg                                                                                                                        >  6:213810/1‑129 (MQ=255)
                                                tttAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACggg                                                                                                                        <  5:213810/129‑1 (MQ=255)
                                                   aaaaCGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGt                                                                                                 >  3:61196/1‑149 (MQ=255)
                                                    aaaCGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCt                                                                                                                 <  6:284225/132‑1 (MQ=255)
                                                    aaaCGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCt                                                                                                                 >  5:284225/1‑132 (MQ=255)
                                                     aaCGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTg                                                                                                                                >  6:167549/1‑116 (MQ=255)
                                                     aaCGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGtt                                                                                                >  8:332730/1‑148 (MQ=255)
                                                        gACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGc                                                                                            <  7:332730/149‑1 (MQ=255)
                                                        gACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGc                                                                                            <  8:1506/149‑1 (MQ=255)
                                                           aaaTAGCCCCTCCACCGTTTAGGTGGCTGCAAACCACCAGGTGTTTCGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGTTCCTCACTAAACCGGGCCGTTTAGCCaa                                                                                         <  6:53241/149‑1 (MQ=255)
                                                           aaaTAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACACCGGGCGATCCTCACTAAACCGGGCCGTTTAGCCaa                                                                                         >  5:301954/1‑149 (MQ=255)
                                                             aTAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACg                                                                                       <  7:128401/149‑1 (MQ=255)
                                                                gCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGtt                                                                                     >  1:3396/1‑148 (MQ=255)
                                                                  cccAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCCCAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGCCAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTaa                                                                                  >  3:333553/1‑149 (MQ=255)
                                                                    cAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGc                                                                                >  1:93101/1‑149 (MQ=255)
                                                                         ccGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAGGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCttttt                                                                           <  8:126980/149‑1 (MQ=255)
                                                                          cGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTg                                                                          <  3:200231/149‑1 (MQ=255)
                                                                            ttAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGc                                                                                            <  8:211988/129‑1 (MQ=255)
                                                                               aCGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTGAATCAGCACAGAGGCGAGAAAAGGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAg                                                                     <  8:186769/149‑1 (MQ=255)
                                                                               aCGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTGAATCAGCACAGAGGCGAGAAAAGGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAg                                                                     <  6:354466/149‑1 (MQ=255)
                                                                                cGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGc                                                                    <  7:319375/149‑1 (MQ=255)
                                                                                 gTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGca                                                                   >  7:267180/1‑149 (MQ=255)
                                                                                     cTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAAt                                                               <  3:246844/149‑1 (MQ=255)
                                                                                     cTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAAt                                                               <  7:67115/149‑1 (MQ=255)
                                                                                      tGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAACTCAGCACAGAGGGGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGCTCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATg                                                              >  2:227101/1‑149 (MQ=255)
                                                                                      tGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCTCAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATg                                                              >  8:112822/1‑149 (MQ=255)
                                                                                      tGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATg                                                              >  4:251312/1‑149 (MQ=255)
                                                                                      tGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATg                                                              >  1:155685/1‑149 (MQ=255)
                                                                                      tGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATg                                                              >  4:257184/1‑149 (MQ=255)
                                                                                        cAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGt                                                             <  8:267180/148‑1 (MQ=255)
                                                                                        cAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGt                                                             >  5:369925/1‑148 (MQ=255)
                                                                                         aaaCCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTa                                                                                   >  4:13784/1‑125 (MQ=255)
                                                                                         aaaCCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTa                                                                                   <  3:13784/125‑1 (MQ=255)
                                                                                         aaaCCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCttttt                                                                           >  7:253533/1‑133 (MQ=255)
                                                                                         aaaCCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCttttt                                                                           <  8:253533/133‑1 (MQ=255)
                                                                                         aaaCCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGTaa                                                           >  4:332263/1‑149 (MQ=255)
                                                                                         aaaCCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGTaa                                                           >  5:292917/1‑149 (MQ=255)
                                                                                           accaccAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGTAATg                                                         <  4:61196/149‑1 (MQ=255)
                                                                                           accaccAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGTAATg                                                         >  5:264665/1‑149 (MQ=255)
                                                                                           accaccAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGTAATg                                                         <  2:155685/149‑1 (MQ=255)
                                                                                            ccaccaGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGTAATgg                                                        >  5:61402/1‑149 (MQ=255)
                                                                                             caccaGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGTAATGGc                                                       <  8:32625/149‑1 (MQ=255)
                                                                                              accaGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGTAATGGCg                                                      >  5:274245/1‑149 (MQ=255)
                                                                                                caGGTGTTACGTCACTCTTTCCTTGCGTAAATCAGCAGAGAGGCGAGAAAAAGGGGGAATGTGAGTAGTTCTGACAAAGGGCGGTCCTCACTAAACCGGGCCGTTTAGCCAAGGTTTAAGCTTTTTGTGAAGCACAATGTAATGGAGta                                                    <  2:375083/149‑2 (MQ=255)
                                                                                                   gtgtTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTga                                                                         <  2:383756/125‑1 (MQ=255)
                                                                                                   gtgtTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTga                                                                         >  1:383756/1‑125 (MQ=255)
                                                                                                   gtgtTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGTAATGGCGTCGc                                                  >  7:37682/1‑148 (MQ=255)
                                                                                                     gtTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCttttt                                                                           <  1:167203/121‑1 (MQ=255)
                                                                                                     gtTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCttttt                                                                           >  2:167203/1‑121 (MQ=255)
                                                                                                            cccGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGTAATGGCGTCGCCATATtgctg                                        <  2:93101/149‑1 (MQ=255)
                                                                                                              cGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGAt                                                                                                                    >  1:210630/1‑71 (MQ=255)
                                                                                                              cGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGAt                                                                                                                    <  2:210630/71‑1 (MQ=255)
                                                                                                               gCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGTAATGGCGTCGCCATATTGCTGCaa                                     >  3:83869/1‑149 (MQ=255)
                                                                                                                   tCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAg                                                                                 <  8:336166/101‑1 (MQ=255)
                                                                                                                   tCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGTAATGGCGTCGCCATATTGCTGCAATTc                                  <  5:38319/148‑1 (MQ=255)
                                                                                                                     cATGCGTAAATCAGCACAGATGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGTAATGGCGTCGCCATATTGCTGCAATTCTa                                <  4:1352/148‑1 (MQ=255)
                                                                                                                     cATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGTAATGGCGTCGCCATATTGCTGCAATTCTa                                <  6:301954/148‑1 (MQ=255)
                                                                                                                     cATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGTAATGGCGTCGCCATATTGCTGCAATTCTa                                <  6:61402/148‑1 (MQ=255)
                                                                                                                          gTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGTAATGGCGTCGCCATATTGCTGCAATTCTa                                >  5:341239/1‑143 (MQ=255)
                                                                                                                          gTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGTAATGGCGTCGCCATATTGCTGCAATTCTa                                <  6:341239/143‑1 (MQ=255)
                                                                                                                          gTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGTAATGGCGTCGCCATATTGCTGCAATTCTAGTGTc                           >  3:56766/1‑148 (MQ=255)
                                                                                                                                  gCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGTAATGGCGTCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATg                  <  1:293358/149‑1 (MQ=255)
                                                                                                                                  gCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGTAATGGCGTCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATg                  >  2:181089/1‑149 (MQ=255)
                                                                                                                                   cacaGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGTAATGGCGTCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGc                 >  7:149037/1‑149 (MQ=255)
                                                                                                                                           cGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGTAATGGCGTCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGAtgttgt         <  3:332263/149‑1 (MQ=255)
                                                                                                                                            gagaAAAAGGGCGAAAGAGAGTAGTTCTGCCAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGTAATGGCGTCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGATgttgtt        <  4:333553/149‑1 (MQ=255)
                                                                                                                                            gagaAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGTAATGGCGTCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGAtgttgt         >  6:83949/1‑148 (MQ=255)
                                                                                                                                            gagaAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGTAATGGCGTCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGAtgttgt         >  7:278960/1‑148 (MQ=255)
                                                                                                                                             agaAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGTAATGGCGTCGCCATATtgct                                         <  2:57388/115‑1 (MQ=255)
                                                                                                                                             agaAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGTAATGGCGTCGCCATATtgct                                         >  1:57388/1‑115 (MQ=255)
                                                                                                                                              gaAAAAGGGCGAAAGAGAGTAGTACTGACAACGGGCGATCCTCACCAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGTAAAGGCGTCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGATGTTGTTAg      >  6:269871/1‑149 (MQ=255)
                                                                                                                                                 aaaGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGTAATGGCGTCGCCATATTGCTGCAATTCTAgt                              >  7:372652/1‑122 (MQ=255)
                                                                                                                                                 aaaGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGTAATGGCGTCGCCATATTGCTGCAATTCTAgt                              <  8:372652/122‑1 (MQ=255)
                                                                                                                                                 aaaGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGTAATGGCGTCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGATGTTGTTAGGaa   >  2:2959/1‑149 (MQ=255)
                                                                                                                                                   aGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCCCTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGTAATGGCGTCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAAt  >  5:398893/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCCAACGTTTAAGCTTTTTGAGAAGCACAATGTAATGGCGTCGCCATATTGCTGCAATTCTAGTGTCAAAAAAATGCGATGTTGTTAGGAAT  >  NC_000913/4599269‑4599563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: